USP25 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  USP25
Gene Name:  ubiquitin specific peptidase 25
Original Clone ID:
ccsbBroadEn_03088
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC075792.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00514647
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3166nts
Open reading frame: 1 to 3166

ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTT
TTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTG
AAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAG
ACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATAC
ATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGAT
GATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGGGCATTCAGGGAGACT
GGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCAGAGAAT
AAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGAT
AGAAAAAGACAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTT
AGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTAC
AAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCT
TTTATGCGTGAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTT
GATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTCAAATCAAATGACTCACAGCAG
CAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATG
AAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTAT
GGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTT
GGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCA
ATGATTGAAGGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAG
CATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAAT
CAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATAT
TTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAG
AGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTAAGCTATGGT
TCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCA
AGTAAACCTGTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGT
TCCATACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCTTCAGAA
CTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAA
CCATTTACTCAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATA
ACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAA
AATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATG
TACTCTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAA
GGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGG
ATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCT
TTTGGTGGTTATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAG
TTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACA
TTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTA
GAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAG
AAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGAGACCCAGAATAT
CTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATA
ATTACCAAGGCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCA
ATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCAGAAACC
GATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATT
ATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT
CACAACATAATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAAC
TTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTAT
CTCATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTC
ATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGACATGAT
GAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCA
GAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAG
TTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTA
GCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACAC
CTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAA
AGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTT
GCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGA

Protein sequence predicted based on nucleotide sequence

MTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAK
TPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRET
GITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWF
SAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYV
DPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFY
GRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQE
HWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIK
RLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSG
SIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHI
TEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHE
GQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQ
FLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQ
KLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSA
IKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERC
HNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFL
ICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNE
FIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIK
SFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable