DNASU Plasmid | BCL2L10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: BCL2L10

Gene Name: BCL2-like 10 (apoptosis facilitator)

Original Clone ID:

ccsbBroadEn_02291

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC093826.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514965

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 613nts

Open reading frame: 1 to 613

Reference Sequence Alignment

ATGGTTGACCAGTTGCGGGAGCGCACCACCATGGCCGACCCGCTGCGGGAGCGCACCGAG
CTGTTGCTGGCCGACTACCTGGGGTACTGCGCCCGGGAACCCGGCACCCCCGAGCCGGCG
CCATCCACGCCCGAGGCCGCCGTGCTGCGCTCCGCGGCCGCCAGGTTACGGCAGATTCAC
CGGTCCTTTTTCTCCGCCTACCTCGGCTACCCCGGGAACCGCTTCGAGCTGGTGGCGCTG
ATGGCGGATTCCGTGCTCTCCGACAGCCCCGGCCCCACCTGGGGCAGAGTGGTGACGCTC
GTGACCTTCGCAGGGACGCTGCTGGAGAGAGGGCCGCTGGTGACCGCCCGGTGGAAGAAG
TGGGGCTTCCAGCCGCGGCTAAAGGAGCAGGAGGGCGACGTCGCCCGGGACTGCCAGCGC
CTGGTGGCCTTGCTGAGCTCGCGGCTCATGGGGCAGCACCGCGCCTGGCTGCAGGCTCAG
GGCGGCTGGGATGGCTTTTGTCACTTCTTCAGGACCCCCTTTCCACTGGCTTTTTGGAGA
AAACAGCTGGTCCAGGCTTTTCTGTCATGCTTGTTAACAACAGCCTTCATTTATCTCTGG
ACACGATTATTA

Protein sequence predicted based on nucleotide sequence

MVDQLRERTTMADPLRERTELLLADYLGYCAREPGTPEPAPSTPEAAVLRSAAARLRQIH
RSFFSAYLGYPGNRFELVALMADSVLSDSPGPTWGRVVTLVTFAGTLLERGPLVTARWKK
WGFQPRLKEQEGDVARDCQRLVALLSSRLMGQHRAWLQAQGGWDGFCHFFRTPFPLAFWR
KQLVQAFLSCLLTTAFIYLWTRLL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

BCL2L10

Symbol Nomenclature :

BCL2L10

SYNONYM :

BCL-B; Boo; Diva

Designation :

anti-apoptotic protein NrH|apoptosis regulator Bcl-B|bcl-2-like protein 10|bcl2-L-10

Full Nomenclature :

BCL2-like 10 (apoptosis facilitator)

GENEID :

10017

HGNC :

993

MIM :

606910

Vega :

OTTHUMG00000131893

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00514965        ATGGTTGACCAGTTGCGGGAGCGCACCACCATGGCCGACCCGCTGCGGGAGCGCACCGAG
NM_020396.2         ATGGTTGACCAGTTGCGGGAGCGCACCACCATGGCCGACCCGCTGCGGGAGCGCACCGAG
XM_005254103.1      ATGGTTGACCAGTTGCGGGAGCGCACCACCATGGCCGACCCGCTGCGGGAGCGCACCGAG
                    ************************************************************

HsCD00514965        CTGTTGCTGGCCGACTACCTGGGGTACTGCGCCCGGGAACCCGGCACCCCCGAGCCGGCG
NM_020396.2         CTGTTGCTGGCCGACTACCTGGGGTACTGCGCCCGGGAACCCGGCACCCCCGAGCCGGCG
XM_005254103.1      CTGTTGCTGGCCGACTACCTGGGGTACTGCGCCCGGGAACCCGGCACCCCCGAGCCGGCG
                    ************************************************************

HsCD00514965        CCATCCACGCCCGAGGCCGCCGTGCTGCGCTCCGCGGCCGCCAGGTTACGGCAGATTCAC
NM_020396.2         CCATCCACGCCCGAGGCCGCCGTGCTGCGCTCCGCGGCCGCCAGGTTACGGCAGATTCAC
XM_005254103.1      CCATCCACGCCCGAGGCCGCCGTGCTGCGCTCCGCGGCCGCCAGGTTACGGCAGATTCAC
                    ************************************************************

HsCD00514965        CGGTCCTTTTTCTCCGCCTACCTCGGCTACCCCGGGAACCGCTTCGAGCTGGTGGCGCTG
NM_020396.2         CGGTCCTTTTTCTCCGCCTACCTCGGCTACCCCGGGAACCGCTTCGAGCTGGTGGCGCTG
XM_005254103.1      CGGTCCTTTTTCTCCGCCTACCTCGGCTACCCCGGGAACCGCTTCGAGCTGGTGGCGCTG
                    ************************************************************

HsCD00514965        ATGGCGGATTCCGTGCTCTCCGACAGCCCCGGCCCCACCTGGGGCAGAGTGGTGACGCTC
NM_020396.2         ATGGCGGATTCCGTGCTCTCCGACAGCCCCGGCCCCACCTGGGGCAGAGTGGTGACGCTC
XM_005254103.1      ATGGCGGATTCCGTGCTCTCCGACAGCCCCGGCCCCACCTGGGGCAGAGTGGTGACGCTC
                    ************************************************************

HsCD00514965        GTGACCTTCGCAGGGACGCTGCTGGAGAGAGGGCCGCTGGTGACCGCCCGGTGGAAGAAG
NM_020396.2         GTGACCTTCGCAGGGACGCTGCTGGAGAGAGGGCCGCTGGTGACCGCCCGGTGGAAGAAG
XM_005254103.1      GTGACCTTCGCAGGGACGCTGCTGGAGAGAGGGCCGCTGGTGACCGCCCGGTGGAAGAAG
                    ************************************************************

HsCD00514965        TGGGGCTTCCAGCCGCGGCTAAAGGAGCAGGAGGGCGACGTCGCCCGGGACTGCCAGCGC
NM_020396.2         TGGGGCTTCCAGCCGCGGCTAAAGGAGCAGGAGGGCGACGTCGCCCGGGACTGCCAGCGC
XM_005254103.1      TGGGGCTTCCAGCCGCGGCTAAAGGAGCAGGAGGGCGACGTCGCCCGGGACTGCCAGCGC
                    ************************************************************

HsCD00514965        CTGGTGGCCTTGCTGAGCTCGCGGCTCATGGGGCAGCACCGCGCCTGGCTGCAGGCTCAG
NM_020396.2         CTGGTGGCCTTGCTGAGCTCGCGGCTCATGGGGCAGCACCGCGCCTGGCTGCAGGCTCAG
XM_005254103.1      CTGGTGGCCTTGCTGAGCTCGCGGCTCATGGGGCAGCACCGCGCCTGGCTGCAGGCTCAG
                    ************************************************************

HsCD00514965        GGCGGCTGG---------------------------------------------------
NM_020396.2         GGCGGCTGG---------------------------------------------------
XM_005254103.1      GGCGGCTGGGTGAGCACGCGGCGGACACCGGGACACGGGGCGGGACGGGCAGCCGGGAAG
                    *********                                                   

HsCD00514965        -------------------GATGGCTTTTGTCACTTCTTCAGGACCCCCTTTCCACTGGC
NM_020396.2         -------------------GATGGCTTTTGTCACTTCTTCAGGACCCCCTTTCCACTGGC
XM_005254103.1      CGCCCACGAGGCTGGCACGGATGGCTTTTGTCACTTCTTCAGGACCCCCTTTCCACTGGC
                                       *****************************************

HsCD00514965        TTTTTGGAGAAAACAGCTGGTCCAGGCTTTTCTGTCATGCTTGTTAACAACAGCCTTCAT
NM_020396.2         TTTTTGGAGAAAACAGCTGGTCCAGGCTTTTCTGTCATGCTTGTTAACAACAGCCTTCAT
XM_005254103.1      TTTTTGGAGAAAACAGCTGGTCCAGGCTTTTCTGTCATGCTTGTTAACAACAGCCTTCAT
                    ************************************************************

HsCD00514965        TTATCTCTGGACACGATTATTA--------------------------------------
NM_020396.2         TTATCTCTGGACACGATTATTATGA-----------------------------------
XM_005254103.1      TTATCTCTGGACACGATTATTATGAGTTTTAAAACTTTTAACCCGCTTCTACCTGCCCAA
                    **********************                                      

HsCD00514965        ------
NM_020396.2         ------
XM_005254103.1      CTGTGA