DNASU Plasmid | PELI2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PELI2

Gene Name: pellino E3 ubiquitin protein ligase family member 2

Original Clone ID:

ccsbBroadEn_08713

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC009476.2

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515409

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1261nts

Open reading frame: 1 to 1261

Reference Sequence Alignment

ATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGAGCCAGTGAAATACGGG
GAGCTGGTGGTGCTCGGGTACAATGGTGCTTTACCCAATGGAGATAGAGGACGGAGGAAA
AGTAGATTTGCCCTCTACAAGCGGCCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCAT
GTGATATCCACGCCCCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCC
TACACTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGATACGGAT
ATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGTTGTCACAGACACGATT
TCTGGCAGCCAGAACACGGACGAAGCCCAGATCACACAGAGCACCATATCCAGGTTCGCC
TGCAGGATCGTGTGCGACAGGAATGAACCTTACACAGCACGGATATTCGCCGCCGGATTT
GACTCTTCCAAAAACATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGC
CACATGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGCTTCACC
GAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGGAGATGTGTACACCTTG
CGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGCTGGTGGAAAGTGAGACCAACGTCCTG
CAGGACGGCTCCCTCATTGACCTGTGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGG
CTTTTTCATACTCCAACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCC
CGGCCTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGGAAAGAG
GTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCACGTGCACGGGTACCAC
AACTGGGGCCATCGGAGTGACACGGAGGCCAACGAGAGGGAGTGTCCCATGTGCAGGACT
GTGGGCCCCTATGTGCCTCTCTGGCTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGA
CCGCCAACTCATGCTTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATAC
TGGTCTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCTTTCTGT
GCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTTCCAAGGACCAATTGAC

Protein sequence predicted based on nucleotide sequence

MFSPGQEEHCAPNKEPVKYGELVVLGYNGALPNGDRGRRKSRFALYKRPKANGVKPSTVH
VISTPQASKAISCKGQHSISYTLSRNQTVVVEYTHDKDTDMFQVGRSTESPIDFVVTDTI
SGSQNTDEAQITQSTISRFACRIVCDRNEPYTARIFAAGFDSSKNIFLGEKAAKWKNPDG
HMDGLTTNGVLVMHPRGGFTEESQPGVWREISVCGDVYTLRETRSAQQRGKLVESETNVL
QDGSLIDLCGATLLWRTADGLFHTPTQKHIEALRQEINAARPQCPVGLNTLAFPSINRKE
VVEEKQPWAYLSCGHVHGYHNWGHRSDTEANERECPMCRTVGPYVPLWLGCEAGFYVDAG
PPTHAFTPCGHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPID

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

1 error

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PELI2

Symbol Nomenclature :

PELI2

Designation :

E3 ubiquitin-protein ligase pellino homolog 2|pellino homolog 2|pellino-2|protein pellino homolog 2

Full Nomenclature :

pellino E3 ubiquitin protein ligase family member 2

GENEID :

57161

HGNC :

8828

MIM :

614798

Vega :

OTTHUMG00000152336

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515409      ATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGAGCCAGTGAAATACGGG
NM_021255.2       ATGTTTTCCCCTGGCCAGGAGGAACACTGCGCCCCCAATAAGGAGCCAGTGAAATACGGG
                  ************************************************************

HsCD00515409      GAGCTGGTGGTGCTCGGGTACAATGGTGCTTTACCCAATGGAGATAGAGGACGGAGGAAA
NM_021255.2       GAGCTGGTGGTGCTCGGGTACAATGGTGCTTTACCCAATGGAGATAGAGGACGGAGGAAA
                  ************************************************************

HsCD00515409      AGTAGATTTGCCCTCTACAAGCGGCCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCAT
NM_021255.2       AGTAGATTTGCCCTCTACAAGCGGCCCAAGGCAAATGGTGTCAAACCCAGCACCGTCCAT
                  ************************************************************

HsCD00515409      GTGATATCCACGCCCCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCC
NM_021255.2       GTGATATCCACGCCCCAGGCATCCAAGGCTATCAGCTGCAAAGGTCAACACAGTATATCC
                  ************************************************************

HsCD00515409      TACACTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGATACGGAT
NM_021255.2       TACACTTTGTCAAGGAATCAGACTGTGGTGGTGGAGTACACACATGATAAGGATACGGAT
                  ************************************************************

HsCD00515409      ATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGTTGTCACAGACACGATT
NM_021255.2       ATGTTTCAGGTGGGCAGATCAACAGAAAGCCCTATCGACTTCGTTGTCACAGACACGATT
                  ************************************************************

HsCD00515409      TCTGGCAGCCAGAACACGGACGAAGCCCAGATCACACAGAGCACCATATCCAGGTTCGCC
NM_021255.2       TCTGGCAGCCAGAACACGGACGAAGCCCAGATCACACAGAGCACCATATCCAGGTTCGCC
                  ************************************************************

HsCD00515409      TGCAGGATCGTGTGCGACAGGAATGAACCTTACACAGCACGGATATTCGCCGCCGGATTT
NM_021255.2       TGCAGGATCGTGTGCGACAGGAATGAACCTTACACAGCACGGATATTCGCCGCCGGATTT
                  ************************************************************

HsCD00515409      GACTCTTCCAAAAACATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGC
NM_021255.2       GACTCTTCCAAAAACATATTTCTTGGAGAAAAGGCAGCAAAGTGGAAAAACCCCGACGGC
                  ************************************************************

HsCD00515409      CACATGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGCTTCACC
NM_021255.2       CACATGGATGGGCTCACTACTAATGGCGTCCTGGTGATGCATCCACGAGGGGGCTTCACC
                  ************************************************************

HsCD00515409      GAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGGAGATGTGTACACCTTG
NM_021255.2       GAGGAGTCCCAGCCCGGGGTCTGGCGCGAGATCTCTGTCTGTGGAGATGTGTACACCTTG
                  ************************************************************

HsCD00515409      CGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGCTGGTGGAAAGTGAGACCAACGTCCTG
NM_021255.2       CGAGAAACCAGGTCGGCCCAGCAACGAGGAAAGCTGGTGGAAAGTGAGACCAACGTCCTG
                  ************************************************************

HsCD00515409      CAGGACGGCTCCCTCATTGACCTGTGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGG
NM_021255.2       CAGGACGGCTCCCTCATTGACCTGTGTGGGGCCACTCTCCTCTGGAGAACAGCAGATGGG
                  ************************************************************

HsCD00515409      CTTTTTCATACTCCAACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCC
NM_021255.2       CTTTTTCATACTCCAACTCAGAAGCACATAGAAGCCCTCCGGCAGGAGATTAACGCCGCC
                  ************************************************************

HsCD00515409      CGGCCTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGGAAAGAG
NM_021255.2       CGGCCTCAGTGTCCTGTGGGGCTCAACACCCTGGCCTTCCCCAGCATCAACAGGAAAGAG
                  ************************************************************

HsCD00515409      GTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCACGTGCACGGGTACCAC
NM_021255.2       GTGGTGGAGGAGAAGCAGCCCTGGGCATATCTCAGTTGTGGCCACGTGCACGGGTACCAC
                  ************************************************************

HsCD00515409      AACTGGGGCCATCGGAGTGACACGGAGGCCAACGAGAGGGAGTGTCCCATGTGCAGGACT
NM_021255.2       AACTGGGGCCATCGGAGTGACACGGAGGCCAACGAGAGGGAGTGTCCCATGTGCAGGACT
                  ************************************************************

HsCD00515409      GTGGGCCCCTATGTGCCTCTCTGGCTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGA
NM_021255.2       GTGGGCCCCTATGTGCCTCTCTGGCTTGGCTGTGAGGCAGGATTTTATGTAGACGCAGGA
                  ************************************************************

HsCD00515409      CCGCCAACTCATGCTTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATAC
NM_021255.2       CCGCCAACTCATGCTTTCACTCCCTGTGGACACGTGTGCTCGGAGAAGTCTGCAAAATAC
                  ************************************************************

HsCD00515409      TGGTCTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCTTTCTGT
NM_021255.2       TGGTCTCAGATCCCGTTGCCTCATGGAACTCATGCATTTCACGCTGCTTGCCCTTTCTGT
                  ************************************************************

HsCD00515409      GCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTTCCAAGGACCAATTGAC
NM_021255.2       GCTACACAGCTGGTTGGGGAGCAAAACTGCATCAAATTAATTTTCCAAGGTCCAATTGAC
                  **************************************************:*********

HsCD00515409      ---
NM_021255.2       TGA

Pathways

Reactome :

Activated TLR4 signalling

Reactome :

Cytokine Signaling in Immune system

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Interleukin-1 signaling

Reactome :

IRAK1 recruits IKK complex

Reactome :

IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation

Reactome :

MyD88 cascade initiated on plasma membrane

Reactome :

MyD88 dependent cascade initiated on endosome

Reactome :

MyD88:Mal cascade initiated on plasma membrane

Reactome :

Signaling by Interleukins

Reactome :

Toll Like Receptor 10 (TLR10) Cascade

Reactome :

Toll Like Receptor 2 (TLR2) Cascade

Reactome :

Toll Like Receptor 4 (TLR4) Cascade

Reactome :

Toll Like Receptor 5 (TLR5) Cascade

Reactome :

Toll Like Receptor 7/8 (TLR7/8) Cascade

Reactome :

Toll Like Receptor 9 (TLR9) Cascade

Reactome :

Toll Like Receptor TLR1:TLR2 Cascade

Reactome :

Toll Like Receptor TLR6:TLR2 Cascade

Reactome :

Toll-Like Receptors Cascades

Reactome :

TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation

WikiPathway :

Regulation of toll-like receptor signaling pathway

Protein

HPRD :

17835

Clone

Cloning Information :

669361

Original Clone ID :

ccsbBroadEn_08713

Organism

Species Specific ID:

57161

Genome

Chromosome :

Map Location :

14q21

Ensembl :

ENSG00000139946

Reagents

Labome :

PELI2-antibody