Original Clone ID:
ccsbBroadEn_00784
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC006499.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00515464
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACC
ATCCAGCTGATCCAGAACCATTTTGTGGACGAATACGACCCCACTATAGAGGATTCCTAC
CGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGACATCCTGGATACCGCCGGC
CAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGT
GTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAGATC
AAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGTGACCTG
GCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCC
TACATCGAGACCTCGGCCAAGACCCGGCAGGGCAGCCGCTCTGGCTCTAGCTCCAGCTCC
GGGACCCTCTGGGACCCCCCGGGACCCATG
Protein sequence predicted based on nucleotide sequence
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDL AARTVESRQAQDLARSYGIPYIETSAKTRQGSRSGSSSSSGTLWDPPGPM
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
Gene
Gene Symbol :
HRAS
Symbol Nomenclature :
HRAS
SYNONYM :
C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; K-RAS; N-RAS; RASH1
Designation :
GTP- and GDP-binding peptide B|GTPase HRas|H-Ras-1|Ha-Ras1 proto-oncoprotein|Ras family small GTP binding protein H-Ras|c-has/bas p21 protein|c-ras-Ki-2 activated oncogene|p19 H-RasIDX protein|p21ras|transformation gene: oncogene HAMSV|transforming protein p21|v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Full Nomenclature :
Harvey rat sarcoma viral oncogene homolog
GENEID :
HGNC :
MIM :
Vega :
Target GenBank:
None
Reference Sequence Alignment
HsCD00515464 ATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACC NM_176795.3 ATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACC ************************************************************ HsCD00515464 ATCCAGCTGATCCAGAACCATTTTGTGGACGAATACGACCCCACTATAGAGGATTCCTAC NM_176795.3 ATCCAGCTGATCCAGAACCATTTTGTGGACGAATACGACCCCACTATAGAGGATTCCTAC ************************************************************ HsCD00515464 CGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGACATCCTGGATACCGCCGGC NM_176795.3 CGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGACATCCTGGATACCGCCGGC ************************************************************ HsCD00515464 CAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGT NM_176795.3 CAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGT ************************************************************ HsCD00515464 GTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAGATC NM_176795.3 GTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAGATC ************************************************************ HsCD00515464 AAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGTGACCTG NM_176795.3 AAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGTGACCTG ************************************************************ HsCD00515464 GCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCC NM_176795.3 GCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCC ************************************************************ HsCD00515464 TACATCGAGACCTCGGCCAAGACCCGGCAGGGCAGCCGCTCTGGCTCTAGCTCCAGCTCC NM_176795.3 TACATCGAGACCTCGGCCAAGACCCGGCAGGGCAGCCGCTCTGGCTCTAGCTCCAGCTCC ************************************************************ HsCD00515464 GGGACCCTCTGGGACCCCCCGGGACCCATG--- NM_176795.3 GGGACCCTCTGGGACCCCCCGGGACCCATGTGA ******************************
Pathways
NCI :
a6b1 and a6b4 Integrin signaling
NCI :
BCR signaling pathway
NCI :
C-MYB transcription factor network
NCI :
CDC42 signaling events
NCI :
Class I PI3K signaling events
NCI :
CXCR3-mediated signaling events
NCI :
Downstream signaling in naïve CD8+ T cells
NCI :
EGF receptor (ErbB1) signaling pathway
NCI :
EGFR-dependent Endothelin signaling events
NCI :
Endothelins
NCI :
EPHB forward signaling
NCI :
EPO signaling pathway
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
GMCSF-mediated signaling events
NCI :
IGF1 pathway
NCI :
IL2-mediated signaling events
NCI :
Insulin Pathway
NCI :
Internalization of ErbB1
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Nongenotropic Androgen signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Plasma membrane estrogen receptor signaling
NCI :
Posttranslational regulation of adherens junction stability and dissassembly
NCI :
Ras signaling in the CD4+ TCR pathway
NCI :
Regulation of Ras family activation
NCI :
SHP2 signaling
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-2-mediated signaling events
NCI :
TCR signaling in naïve CD4+ T cells
NCI :
TCR signaling in naïve CD8+ T cells
NCI :
Trk receptor signaling mediated by PI3K and PLC-gamma
NCI :
Trk receptor signaling mediated by the MAPK pathway
Panther :
Angiogenesis
Panther :
B cell activation
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Integrin signalling pathway
Panther :
p53 pathway feedback loops 2
Panther :
PDGF signaling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
TGF-beta signaling pathway
Panther :
VEGF signaling pathway
Reactome :
Activation of NMDA receptor upon glutamate binding and postsynaptic events
Reactome :
Activation of RAS in B cells
Reactome :
Adaptive Immune System
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Cell surface interactions at the vascular wall
Reactome :
CREB phosphorylation through the activation of Ras
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
Downstream signaling of activated FGFR
Reactome :
EGFR Transactivation by Gastrin
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
Hemostasis
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin receptor SHC signaling
Reactome :
Interleukin-2 signaling
Reactome :
Interleukin-3, 5 and GM-CSF signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
MEK activation
Reactome :
NCAM signaling for neurite out-growth
Reactome :
Neuronal System
Reactome :
Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
p38MAPK events
Reactome :
Post NMDA receptor activation events
Reactome :
Prolonged ERK activation events
Reactome :
RAF activation
Reactome :
RAF phosphorylates MEK
Reactome :
RAF/MAP kinase cascade
Reactome :
Ras activation uopn Ca2+ infux through NMDA receptor
Reactome :
SHC-mediated cascade
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal Transduction
Reactome :
Signaling by constitutively active EGFR
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by FGFR mutants
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Tie2 Signaling
Reactome :
Transmission across Chemical Synapses
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
ErbB signaling pathway
WikiPathway :
G Protein Signaling Pathways
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK Cascade
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
Senescence and Autophagy
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF Beta Signaling Pathway
WikiPathway :
TSH signaling pathway
Protein
SMART domain :
SMART domain :
SMART domain :
HPRD :
01813
Clone
Cloning Information :
Original Clone ID :
ccsbBroadEn_00784
Organism
Species Specific ID:
Genome
Reagents
Labome :