DNASU Plasmid | CYP27C1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CYP27C1

Gene Name: cytochrome P450, family 27, subfamily C, polypeptide 1

Original Clone ID:

ccsbBroadEn_10018

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC039307.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515566

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1117nts

Open reading frame: 1 to 1117

Reference Sequence Alignment

ATGAGAAGCGTATTGAGACAAAGAATTCTGAAACCGAAAGATGTGGCCATTTATTCTGGA
GAAGTCAACCAAGTTATTGCTGACTTAATTAAAAGAATCTACCTCCTCAGGAGCCAGGCA
GAAGATGGAGAAACCGTGACCAATGTCAATGATCTTTTCTTCAAATATTCAATGGAAGGA
GTGGCCACCATCCTTTATGAGAGTCGTTTGGGCTGCCTGGAAAACAGCATCCCACAGCTG
ACTGTGGAATACATCGAGGCCCTGGAGCTCATGTTTAGCATGTTCAAGACCTCCATGTAT
GCAGGCGCCATCCCCAGATGGCTTCGCCCCTTCATCCCAAAGCCCTGGCGGGAATTCTGC
AGGTCCTGGGATGGACTCTTCAAATTCAGCCAAATTCATGTTGACAACAAGTTGAGGGAC
ATACAGTACCAAATGGACCGAGGCCGGAGGGTGAGCGGGGGACTTCTCACATACCTCTTC
CTTAGCCAGGCTCTGACGCTGCAGGAGATCTACGCCAACGTGACTGAGATGCTGCTGGCC
GGCGTCGACACGACGTCCTTCACCTTGTCTTGGACTGTGTACATCCTGGCAAGGCACCCA
GAAGTGCAGCAGACGGTGTACCGGGAGATTGTGAAGAATTTAGGGGAAAGGCATGTTCCA
ACTGCAGCTGATGTCCCCAAGGTCCCGCTGGTCAGAGCTCTCCTTAAGGAAACCCTGAGG
CTGTTTCCAGTGCTGCCAGGGAACGGCCGGGTCACCCAGGAAGACCTGGTTATTGGCGGG
TATCTGATTCCGAAAGGCACCCAGCTGGCCCTTTGCCACTATGCCACATCGTACCAGGAT
GAGAACTTCCCTCGGGCCAAGGAGTTCCGGCCTGAGCGCTGGCTGCGGAAAGGAGACTTA
GATAGAGTTGACAATTTTGGATCCATCCCCTTTGGTCATGGGGTTCGCAGCTGCATAGGG
CGGAGAATTGCAGAACTGGAGATTCACCTCGTCGTGATCCAGTTGCTTCAACATTTTGAG
ATCAAAACATCTTCTCAGACCAATGCTGTTCATGCAAAAACCCACGGGCTCCTGACGCCA
GGGGGGCCCATCCACGTGCGATTTGTTAACAGAAAG

Protein sequence predicted based on nucleotide sequence

MRSVLRQRILKPKDVAIYSGEVNQVIADLIKRIYLLRSQAEDGETVTNVNDLFFKYSMEG
VATILYESRLGCLENSIPQLTVEYIEALELMFSMFKTSMYAGAIPRWLRPFIPKPWREFC
RSWDGLFKFSQIHVDNKLRDIQYQMDRGRRVSGGLLTYLFLSQALTLQEIYANVTEMLLA
GVDTTSFTLSWTVYILARHPEVQQTVYREIVKNLGERHVPTAADVPKVPLVRALLKETLR
LFPVLPGNGRVTQEDLVIGGYLIPKGTQLALCHYATSYQDENFPRAKEFRPERWLRKGDL
DRVDNFGSIPFGHGVRSCIGRRIAELEIHLVVIQLLQHFEIKTSSQTNAVHAKTHGLLTP
GGPIHVRFVNRK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

CYP27C1

Symbol Nomenclature :

CYP27C1

Designation :

cytochrome P450 27C1|cytochrome P450, family 27, subfamily C, polypeptide 13

Full Nomenclature :

cytochrome P450, family 27, subfamily C, polypeptide 1

GENEID :

339761

HGNC :

33480

Vega :

OTTHUMG00000153400

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515566        ATGAGAAGCGTATTGAGACAAAGAATTCTGAAACCGAAAGATGTGGCCATTTATTCTGGA
NM_001001665.3      ATGAGAAGCGTATTGAGACAAAGAATTCTGAAACCGAAAGATGTGGCCATTTATTCTGGA
                    ************************************************************

HsCD00515566        GAAGTCAACCAAGTTATTGCTGACTTAATTAAAAGAATCTACCTCCTCAGGAGCCAGGCA
NM_001001665.3      GAAGTCAACCAAGTTATTGCTGACTTAATTAAAAGAATCTACCTCCTCAGGAGCCAGGCA
                    ************************************************************

HsCD00515566        GAAGATGGAGAAACCGTGACCAATGTCAATGATCTTTTCTTCAAATATTCAATGGAAGGA
NM_001001665.3      GAAGATGGAGAAACCGTGACCAATGTCAATGATCTTTTCTTCAAATATTCAATGGAAGGA
                    ************************************************************

HsCD00515566        GTGGCCACCATCCTTTATGAGAGTCGTTTGGGCTGCCTGGAAAACAGCATCCCACAGCTG
NM_001001665.3      GTGGCCACCATCCTTTATGAGAGTCGTTTGGGCTGCCTGGAAAACAGCATCCCACAGCTG
                    ************************************************************

HsCD00515566        ACTGTGGAATACATCGAGGCCCTGGAGCTCATGTTTAGCATGTTCAAGACCTCCATGTAT
NM_001001665.3      ACTGTGGAATACATCGAGGCCCTGGAGCTCATGTTTAGCATGTTCAAGACCTCCATGTAT
                    ************************************************************

HsCD00515566        GCAGGCGCCATCCCCAGATGGCTTCGCCCCTTCATCCCAAAGCCCTGGCGGGAATTCTGC
NM_001001665.3      GCAGGCGCCATCCCCAGATGGCTTCGCCCCTTCATCCCAAAGCCCTGGCGGGAATTCTGC
                    ************************************************************

HsCD00515566        AGGTCCTGGGATGGACTCTTCAAATTCAGCCAAATTCATGTTGACAACAAGTTGAGGGAC
NM_001001665.3      AGGTCCTGGGATGGACTCTTCAAATTCAGCCAAATTCATGTTGACAACAAGTTGAGGGAC
                    ************************************************************

HsCD00515566        ATACAGTACCAAATGGACCGAGGCCGGAGGGTGAGCGGGGGACTTCTCACATACCTCTTC
NM_001001665.3      ATACAGTACCAAATGGACCGAGGCCGGAGGGTGAGCGGGGGACTTCTCACATACCTCTTC
                    ************************************************************

HsCD00515566        CTTAGCCAGGCTCTGACGCTGCAGGAGATCTACGCCAACGTGACTGAGATGCTGCTGGCC
NM_001001665.3      CTTAGCCAGGCTCTGACGCTGCAGGAGATCTACGCCAACGTGACTGAGATGCTGCTGGCC
                    ************************************************************

HsCD00515566        GGCGTCGACACGACGTCCTTCACCTTGTCTTGGACTGTGTACATCCTGGCAAGGCACCCA
NM_001001665.3      GGCGTCGACACGACGTCCTTCACCTTGTCTTGGACTGTGTACCTCCTGGCAAGGCACCCA
                    ******************************************.*****************

HsCD00515566        GAAGTGCAGCAGACGGTGTACCGGGAGATTGTGAAGAATTTAGGGGAAAGGCATGTTCCA
NM_001001665.3      GAAGTGCAGCAGACGGTGTACCGGGAGATTGTGAAGAATTTAGGGGAAAGGCATGTTCCA
                    ************************************************************

HsCD00515566        ACTGCAGCTGATGTCCCCAAGGTCCCGCTGGTCAGAGCTCTCCTTAAGGAAACCCTGAGG
NM_001001665.3      ACTGCAGCTGATGTCCCCAAGGTCCCGCTGGTCAGAGCTCTCCTTAAGGAAACCCTGAGG
                    ************************************************************

HsCD00515566        CTGTTTCCAGTGCTGCCAGGGAACGGCCGGGTCACCCAGGAAGACCTGGTTATTGGCGGG
NM_001001665.3      CTGTTTCCAGTGCTGCCAGGGAACGGCCGGGTCACCCAGGAAGACCTGGTTATTGGCGGG
                    ************************************************************

HsCD00515566        TATCTGATTCCGAAAGGCACCCAGCTGGCCCTTTGCCACTATGCCACATCGTACCAGGAT
NM_001001665.3      TATCTGATTCCGAAAGGCACCCAGCTGGCCCTTTGCCACTATGCCACATCGTACCAGGAT
                    ************************************************************

HsCD00515566        GAGAACTTCCCTCGGGCCAAGGAGTTCCGGCCTGAGCGCTGGCTGCGGAAAGGAGACTTA
NM_001001665.3      GAGAACTTCCCTCGGGCCAAGGAGTTCCGGCCTGAGCGCTGGCTGCGGAAAGGAGACTTA
                    ************************************************************

HsCD00515566        GATAGAGTTGACAATTTTGGATCCATCCCCTTTGGTCATGGGGTTCGCAGCTGCATAGGG
NM_001001665.3      GATAGAGTTGACAATTTTGGATCCATCCCCTTTGGTCATGGGGTTCGCAGCTGCATAGGG
                    ************************************************************

HsCD00515566        CGGAGAATTGCAGAACTGGAGATTCACCTCGTCGTGATCCAGTTGCTTCAACATTTTGAG
NM_001001665.3      CGGAGAATTGCAGAACTGGAGATTCACCTCGTCGTGATCCAGTTGCTTCAACATTTTGAG
                    ************************************************************

HsCD00515566        ATCAAAACATCTTCTCAGACCAATGCTGTTCATGCAAAAACCCACGGGCTCCTGACGCCA
NM_001001665.3      ATCAAAACATCTTCTCAGACCAATGCTGTTCATGCAAAAACCCACGGGCTCCTGACGCCA
                    ************************************************************

HsCD00515566        GGGGGGCCCATCCACGTGCGATTTGTTAACAGAAAG---
NM_001001665.3      GGGGGGCCCATCCACGTGCGATTTGTTAACAGAAAGTAA
                    ************************************

Pathways

Panther :

Vitamin D metabolism and pathway

WikiPathway :

cytochrome P450

WikiPathway :

metapathway biotransformation

Protein

HPRD :

13360

Clone

Cloning Information :

668672

Original Clone ID :

ccsbBroadEn_10018

Organism

Species Specific ID:

339761

Genome

Chromosome :

Map Location :

2q14.3

Ensembl :

ENSG00000186684

Reagents

Labome :

CYP27C1-antibody