DNASU Plasmid | WBP4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: WBP4

Gene Name: WW domain binding protein 4

Original Clone ID:

ccsbBroadEn_02644

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC104879.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515604

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1129nts

Open reading frame: 1 to 1129

Reference Sequence Alignment

ATGGCGGACTACTGGAAGTCACAGCCAAAGAAATTCTGTGATTACTGCAAGTGCTGGATA
GCAGACAATAGGCCTAGTGTTGAATTTCATGAAAGAGGAAAGAATCATAAGGAAAATGTG
GCAAAAAGGATCAGTGAGATTAAACAGAAAAGCCTGGATAAGGCAAAGGAAGAAGAAAAG
GCATCAAAGGAGTTTGCTGCAATGGAGGCAGCTGCCCTGAAAGCATACCAAGAGGATTTG
AAAAGACTTGGCTTAGAGTCAGAAATTTTGGAGCCAAGCATAACACCAGTAACCAGCACT
ATCCCACCTACCTCGACATCAAATCAACAGAAAGAAAAGAAAGAAAAGAAGAAAAGAAAA
AAAGATCCTTCAAAGGGCAGATGGGTAGAAGGCATAACCTCTGAGGGTTACCATTACTAT
TATGATCTTATCTCAGGAGCATCTCAGTGGGAGAAACCTGAAGGATTTCAAGGAGACTTA
AAAAAGACAGCAGTGAAGACCGTTTGGGTAGAAGGTTTAAGTGAAGATGGTTTTACCTAT
TACTATAATACAGAAACAGGAGAATCCAGATGGGAGAAACCTGATGATTTCATTCCACAC
ACTAGTGATCTGCCTTCTAGTAAGGTCAATGAAAATTCACTTGGCACCCTAGATGAATCC
AAATCATCAGATTCGCATAGTGATTCTGATGGGGAACAGGAAGCAGAAGAAGGAGGGGTC
TCTACAGAGACAGAAAAGCCAAAAATAAAGTTTAAGGAAAAAAATAAAAATAGTGATGGA
GGAAGTGACCCAGAAACACAGAAAGAAAAAAGTATTCAGAAACAGAATTCATTAGGTTCA
AATGAAGAAAAATCGAAAACTCTTAAGAAATCAAACCCATATGGAGAATGGCAAGAAATT
AAACAAGAGGTTGAGTCTCATGAGGAGGTAGATTTGGAACTTCCAAGCACTGAAAATGAG
TATGTATCAACTTCAGAAGCTGATGGTGGCGGAGAACCCAAAGTGGTATTTAAAGAAAAA
ACAGTCACTTCTCTTGGAGTTATGGCAGATGGAGTGGCCCCAGTCTTCAAAAAGAGAAGA
ACTGAAAATGGAAAATCTAGAAATTTAAGGCAACGAGGTGATGATCAA

Protein sequence predicted based on nucleotide sequence

MADYWKSQPKKFCDYCKCWIADNRPSVEFHERGKNHKENVAKRISEIKQKSLDKAKEEEK
ASKEFAAMEAAALKAYQEDLKRLGLESEILEPSITPVTSTIPPTSTSNQQKEKKEKKKRK
KDPSKGRWVEGITSEGYHYYYDLISGASQWEKPEGFQGDLKKTAVKTVWVEGLSEDGFTY
YYNTETGESRWEKPDDFIPHTSDLPSSKVNENSLGTLDESKSSDSHSDSDGEQEAEEGGV
STETEKPKIKFKEKNKNSDGGSDPETQKEKSIQKQNSLGSNEEKSKTLKKSNPYGEWQEI
KQEVESHEEVDLELPSTENEYVSTSEADGGGEPKVVFKEKTVTSLGVMADGVAPVFKKRR
TENGKSRNLRQRGDDQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

WBP4

Symbol Nomenclature :

WBP4

SYNONYM :

FBP21

Designation :

Full Nomenclature :

WW domain binding protein 4

GENEID :

11193

HGNC :

12739

MIM :

604981

Vega :

OTTHUMG00000016784

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515604        ------------------------------------------------------------
NM_007187.3         ------------------------------------------------------------
XM_005266245.1      ATGGCTTTTCCAGTTCTCTCTTGCCTTGAGGCTCAAGAACAGTTTGTTTTAACTGAGAGT
                                                                                

HsCD00515604        ---------------------------------ATGGCGGACTACTGGAAGTCACAGCCA
NM_007187.3         ---------------------------------ATGGCGGACTACTGGAAGTCACAGCCA
XM_005266245.1      CTGGGAGGAACTACAGTTTTCCCCAGAGTAGTCTTGGCGGACTACTGGAAGTCACAGCCA
                                                     :**************************

HsCD00515604        AAGAAATTCTGTGATTACTGCAAGTGCTGGATAGCAGACAATAGGCCTAGTGTTGAATTT
NM_007187.3         AAGAAATTCTGTGATTACTGCAAGTGCTGGATAGCAGACAATAGGCCTAGTGTTGAATTT
XM_005266245.1      AAGAAATTCTGTGATTACTGCAAGTGCTGGATAGCAGACAATAGGCCTAGTGTTGAATTT
                    ************************************************************

HsCD00515604        CATGAAAGAGGAAAGAATCATAAGGAAAATGTGGCAAAAAGGATCAGTGAGATTAAACAG
NM_007187.3         CATGAAAGAGGAAAGAATCATAAGGAAAATGTGGCAAAAAGGATCAGTGAGATTAAACAG
XM_005266245.1      CATGAAAGAGGAAAGAATCATAAGGAAAATGTGGCAAAAAGGATCAGTGAGATTAAACAG
                    ************************************************************

HsCD00515604        AAAAGCCTGGATAAGGCAAAGGAAGAAGAAAAGGCATCAAAGGAGTTTGCTGCAATGGAG
NM_007187.3         AAAAGCCTGGATAAGGCAAAGGAAGAAGAAAAGGCATCAAAGGAGTTTGCTGCAATGGAG
XM_005266245.1      AAAAGCCTGGATAAGGCAAAGGAAGAAGAAAAGGCATCAAAGGAGTTTGCTGCAATGGAG
                    ************************************************************

HsCD00515604        GCAGCTGCCCTGAAAGCATACCAAGAGGATTTGAAAAGACTTGGCTTAGAGTCAGAAATT
NM_007187.3         GCAGCTGCCCTGAAAGCATACCAAGAGGATTTGAAAAGACTTGGCTTAGAGTCAGAAATT
XM_005266245.1      GCAGCTGCCCTGAAAGCATACCAAGAGGATTTGAAAAGACTTGGCTTAGAGTCAGAAATT
                    ************************************************************

HsCD00515604        TTGGAGCCAAGCATAACACCAGTAACCAGCACTATCCCACCTACCTCGACATCAAATCAA
NM_007187.3         TTGGAGCCAAGCATAACACCAGTAACCAGCACTATCCCACCTACCTCGACATCAAATCAA
XM_005266245.1      TTGGAGCCAAGCATAACACCAGTAACCAGCACTATCCCACCTACCTCGACATCAAATCAA
                    ************************************************************

HsCD00515604        CAGAAAGAAAAGAAAGAAAAGAAGAAAAGAAAAAAAGATCCTTCAAAGGGCAGATGGGTA
NM_007187.3         CAGAAAGAAAAGAAAGAAAAGAAGAAAAGAAAAAAAGATCCTTCAAAGGGCAGATGGGTA
XM_005266245.1      CAGAAAGAAAAGAAAGAAAAGAAGAAAAGAAAAAAAGATCCTTCAAAGGGCAGATGGGTA
                    ************************************************************

HsCD00515604        GAAGGCATAACCTCTGAGGGTTACCATTACTATTATGATCTTATCTCAGGAGCATCTCAG
NM_007187.3         GAAGGCATAACCTCTGAGGGTTACCATTACTATTATGATCTTATCTCAGGAGCATCTCAG
XM_005266245.1      GAAGGCATAACCTCTGAGGGTTACCATTACTATTATGATCTTATCTCAGGAGCATCTCAG
                    ************************************************************

HsCD00515604        TGGGAGAAACCTGAAGGATTTCAAGGAGACTTAAAAAAGACAGCAGTGAAGACCGTTTGG
NM_007187.3         TGGGAGAAACCTGAAGGATTTCAAGGAGACTTAAAAAAGACAGCAGTGAAGACCGTTTGG
XM_005266245.1      TGGGAGAAACCTGAAGGATTTCAAGGAGACTTAAAAAAGACAGCAGTGAAGACCGTTTGG
                    ************************************************************

HsCD00515604        GTAGAAGGTTTAAGTGAAGATGGTTTTACCTATTACTATAATACAGAAACAGGAGAATCC
NM_007187.3         GTAGAAGGTTTAAGTGAAGATGGTTTTACCTATTACTATAATACAGAAACAGGAGAATCC
XM_005266245.1      GTAGAAGGTTTAAGTGAAGATGGTTTTACCTATTACTATAATACAGAAACAGGAGAATCC
                    ************************************************************

HsCD00515604        AGATGGGAGAAACCTGATGATTTCATTCCACACACTAGTGATCTGCCTTCTAGTAAGGTC
NM_007187.3         AGATGGGAGAAACCTGATGATTTCATTCCACACACTAGTGATCTGCCTTCTAGTAAGGTC
XM_005266245.1      AGATGGGAGAAACCTGATGATTTCATTCCACACACTAGTGATCTGCCTTCTAGTAAGGTC
                    ************************************************************

HsCD00515604        AATGAAAATTCACTTGGCACCCTAGATGAATCCAAATCATCAGATTCGCATAGTGATTCT
NM_007187.3         AATGAAAATTCACTTGGCACCCTAGATGAATCCAAATCATCAGATTCGCATAGTGATTCT
XM_005266245.1      AATGAAAATTCACTTGGCACCCTAGATGAATCCAAATCATCAGATTCGCATAGTGATTCT
                    ************************************************************

HsCD00515604        GATGGGGAACAGGAAGCAGAAGAAGGAGGGGTCTCTACAGAGACAGAAAAGCCAAAAATA
NM_007187.3         GATGGGGAACAGGAAGCAGAAGAAGGAGGGGTCTCTACAGAGACAGAAAAGCCAAAAATA
XM_005266245.1      GATGGGGAACAGGAAGCAGAAGAAGGAGGGGTCTCTACAGAGACAGAAAAGCCAAAAATA
                    ************************************************************

HsCD00515604        AAGTTTAAGGAAAAAAATAAAAATAGTGATGGAGGAAGTGACCCAGAAACACAGAAAGAA
NM_007187.3         AAGTTTAAGGAAAAAAATAAAAATAGTGATGGAGGAAGTGACCCAGAAACACAGAAAGAA
XM_005266245.1      AAGTTTAAGGAAAAAAATAAAAATAGTGATGGAGGAAGTGACCCAGAAACACAGAAAGAA
                    ************************************************************

HsCD00515604        AAAAGTATTCAGAAACAGAATTCATTAGGTTCAAATGAAGAAAAATCGAAAACTCTTAAG
NM_007187.3         AAAAGTATTCAGAAACAGAATTCATTAGGTTCAAATGAAGAAAAATCGAAAACTCTTAAG
XM_005266245.1      AAAAGTATTCAGAAACAGAATTCATTAGGTTCAAATGAAGAAAAATCGAAAACTCTTAAG
                    ************************************************************

HsCD00515604        AAATCAAACCCATATGGAGAATGGCAAGAAATTAAACAAGAGGTTGAGTCTCATGAGGAG
NM_007187.3         AAATCAAACCCATATGGAGAATGGCAAGAAATTAAACAAGAGGTTGAGTCTCATGAGGAG
XM_005266245.1      AAATCAAACCCATATGGAGAATGGCAAGAAATTAAACAAGAGGTTGAGTCTCATGAGGAG
                    ************************************************************

HsCD00515604        GTAGATTTGGAACTTCCAAGCACTGAAAATGAGTATGTATCAACTTCAGAAGCTGATGGT
NM_007187.3         GTAGATTTGGAACTTCCAAGCACTGAAAATGAGTATGTATCAACTTCAGAAGCTGATGGT
XM_005266245.1      GTAGATTTGGAACTTCCAAGCACTGAAAATGAGTATGTATCAACTTCAGAAGCTGATGGT
                    ************************************************************

HsCD00515604        GGCGGAGAACCCAAAGTGGTATTTAAAGAAAAAACAGTCACTTCTCTTGGAGTTATGGCA
NM_007187.3         GGCGGAGAACCCAAAGTGGTATTTAAAGAAAAAACAGTCACTTCTCTTGGAGTTATGGCA
XM_005266245.1      GGCGGAGAACCCAAAGTGGTATTTAAAGAAAAAACAGTCACTTCTCTTGGAGTTATGGCA
                    ************************************************************

HsCD00515604        GATGGAGTGGCCCCAGTCTTCAAAAAGAGAAGAACTGAAAATGGAAAATCTAGAAATTTA
NM_007187.3         GATGGAGTGGCCCCAGTCTTCAAAAAGAGAAGAACTGAAAATGGAAAATCTAGAAATTTA
XM_005266245.1      GATGGAGTGGCCCCAGTCTTCAAAAAGAGAAGAACTGAAAATGGAAAATCTAGAAATTTA
                    ************************************************************

HsCD00515604        AGGCAACGAGGTGATGATCAA---
NM_007187.3         AGGCAACGAGGTGATGATCAATAG
XM_005266245.1      AGGCAACGAGGTGATGATCAATAG
                    *********************