DNASU Plasmid | KLRG2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KLRG2

Gene Name: killer cell lectin-like receptor subfamily G, member 2

Original Clone ID:

ccsbBroadEn_05505

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC104965.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515631

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1228nts

Open reading frame: 1 to 1228

Reference Sequence Alignment

ATGGAGGAGTCTTGGGAGGCTGCGCCCGGAGGCCAAGCCGGGGCAGAGCTCCCAATGGAG
CCCGTGGGAAGCCTGGTCCCCACGCTGGAGCAGCCGCAGGTGCCCGCGAAGGTGCGACAA
CCTGAAGGTCCCGAAAGCAGCCCAAGTCCGGCCGGGGCCGTGGAGAAGGCGGCGGGCGCA
GGCCTGGAGCCCTCGAGCAAGAAAAAGCCGCCTTCGCCTCGCCCCGGGTCCCCGCGCGTG
CCGCCGCTCAGCCTGGGCTACGGGGTCTGCCCCGAGCCGCCGTCACCGGGCCCTGCCTTG
GTCAAGCTGCCCCGGAATGGCGAGGCGCCCGGGGCTGAGCCTGCGCCCAGCGCCTGGGCG
CCCATGGAGCTGCAGGTAGATGTGCGCGTGAAGCCCGTGGGCGCGGCCGGTGGCAGCAGC
ACGCCATCGCCCAGGCCCTCCACGCGCTTCCTCAAGGTGCCGGTGCCCGAGTCCCCTGCC
TTCTCCCGCCACGCGGACCCGGCGCACCAGCTCCTGCTGCGCGCACCATCCCAGGGCGGC
ACGTGGGGCCGCCGCTCGCCGCTGGCTGCAGCCCGGACGGAGAGCGGCTGCGACGCAGAG
GGCCGGGCCAGCCCCGCGGAAGGAAGCGCCGGCTCCCCGGGCTCCCCCACGTGCTGCCGC
TGCAAGGAGCTGGGGCTGGAGAAGGAGGATGCGGCGCTGTTGCCCCGCGCGGGGTTGGAC
GGCGACGAGAAGCTGCCCCGGGCCGTAACGCTTACGGGGCTACCCATGTACGTGAAGTCC
CTGTACTGGGCCCTGGCGTTCATGGCTGTGCTCCTGGCAGTCTCTGGGGTTGTCATTGTG
GTCCTGGCCTCAAGAGCAGGAGCCAGATGCCAGCAGTGCCCCCCAGGCTGGGTGTTGTCC
GAGGAGCACTGTTACTACTTCTCTGCAGAAGCGCAGGCCTGGGAAGCCAGCCAGGCTTTC
TGCTCAGCCTACCACGCTACCCTCCCCCTGCTAAGCCACACCCAGGACTTCCTGGGCAGA
TACCCAGTCTCCAGGCACTCCTGGGTGGGGGCCTGGCGAGGCCCCCAGGGCTGGCACTGG
ATCGACGAGGCCCCACTCCCGCCCCAGCTACTCCCTGAGGACGGCGAGGACAATCTGGAT
ATCAACTGTGGGGCCCTGGAGGAAGGCACGCTGGTGGCTGCAAACTGCAGCACTCCAAGA
CCCTGGGTCTGTGCCAAGGGGACCCAG

Protein sequence predicted based on nucleotide sequence

MEESWEAAPGGQAGAELPMEPVGSLVPTLEQPQVPAKVRQPEGPESSPSPAGAVEKAAGA
GLEPSSKKKPPSPRPGSPRVPPLSLGYGVCPEPPSPGPALVKLPRNGEAPGAEPAPSAWA
PMELQVDVRVKPVGAAGGSSTPSPRPSTRFLKVPVPESPAFSRHADPAHQLLLRAPSQGG
TWGRRSPLAAARTESGCDAEGRASPAEGSAGSPGSPTCCRCKELGLEKEDAALLPRAGLD
GDEKLPRAVTLTGLPMYVKSLYWALAFMAVLLAVSGVVIVVLASRAGARCQQCPPGWVLS
EEHCYYFSAEAQAWEASQAFCSAYHATLPLLSHTQDFLGRYPVSRHSWVGAWRGPQGWHW
IDEAPLPPQLLPEDGEDNLDINCGALEEGTLVAANCSTPRPWVCAKGTQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KLRG2

Symbol Nomenclature :

KLRG2

SYNONYM :

CLEC15B

Designation :

C-type lectin domain family 15 member B|C-type lectin domain family 15, member B|killer cell lectin-like receptor subfamily G member 2

Full Nomenclature :

killer cell lectin-like receptor subfamily G, member 2

GENEID :

346689

HGNC :

24778

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515631      ATGGAGGAGTCTTGGGAGGCTGCGCCCGGAGGCCAAGCCGGGGCAGAGCTCCCAATGGAG
NM_198508.2       ATGGAGGAGTCTTGGGAGGCTGCGCCCGGAGGCCAAGCCGGGGCAGAGCTCCCAATGGAG
                  ************************************************************

HsCD00515631      CCCGTGGGAAGCCTGGTCCCCACGCTGGAGCAGCCGCAGGTGCCCGCGAAGGTGCGACAA
NM_198508.2       CCCGTGGGAAGCCTGGTCCCCACGCTGGAGCAGCCGCAGGTGCCCGCGAAGGTGCGACAA
                  ************************************************************

HsCD00515631      CCTGAAGGTCCCGAAAGCAGCCCAAGTCCGGCCGGGGCCGTGGAGAAGGCGGCGGGCGCA
NM_198508.2       CCTGAAGGTCCCGAAAGCAGCCCAAGTCCGGCCGGGGCCGTGGAGAAGGCGGCGGGCGCA
                  ************************************************************

HsCD00515631      GGCCTGGAGCCCTCGAGCAAGAAAAAGCCGCCTTCGCCTCGCCCCGGGTCCCCGCGCGTG
NM_198508.2       GGCCTGGAGCCCTCGAGCAAGAAAAAGCCGCCTTCGCCTCGCCCCGGGTCCCCGCGCGTG
                  ************************************************************

HsCD00515631      CCGCCGCTCAGCCTGGGCTACGGGGTCTGCCCCGAGCCGCCGTCACCGGGCCCTGCCTTG
NM_198508.2       CCGCCGCTCAGCCTGGGCTACGGGGTCTGCCCCGAGCCGCCGTCACCGGGCCCTGCCTTG
                  ************************************************************

HsCD00515631      GTCAAGCTGCCCCGGAATGGCGAGGCGCCCGGGGCTGAGCCTGCGCCCAGCGCCTGGGCG
NM_198508.2       GTCAAGCTGCCCCGGAATGGCGAGGCGCCCGGGGCTGAGCCTGCGCCCAGCGCCTGGGCG
                  ************************************************************

HsCD00515631      CCCATGGAGCTGCAGGTAGATGTGCGCGTGAAGCCCGTGGGCGCGGCCGGTGGCAGCAGC
NM_198508.2       CCCATGGAGCTGCAGGTAGATGTGCGCGTGAAGCCCGTGGGCGCGGCCGGTGGCAGCAGC
                  ************************************************************

HsCD00515631      ACGCCATCGCCCAGGCCCTCCACGCGCTTCCTCAAGGTGCCGGTGCCCGAGTCCCCTGCC
NM_198508.2       ACGCCATCGCCCAGGCCCTCCACGCGCTTCCTCAAGGTGCCGGTGCCCGAGTCCCCTGCC
                  ************************************************************

HsCD00515631      TTCTCCCGCCACGCGGACCCGGCGCACCAGCTCCTGCTGCGCGCACCATCCCAGGGCGGC
NM_198508.2       TTCTCCCGCCACGCGGACCCGGCGCACCAGCTCCTGCTGCGCGCACCATCCCAGGGCGGC
                  ************************************************************

HsCD00515631      ACGTGGGGCCGCCGCTCGCCGCTGGCTGCAGCCCGGACGGAGAGCGGCTGCGACGCAGAG
NM_198508.2       ACGTGGGGCCGCCGCTCGCCGCTGGCTGCAGCCCGGACGGAGAGCGGCTGCGACGCAGAG
                  ************************************************************

HsCD00515631      GGCCGGGCCAGCCCCGCGGAAGGAAGCGCCGGCTCCCCGGGCTCCCCCACGTGCTGCCGC
NM_198508.2       GGCCGGGCCAGCCCCGCGGAAGGAAGCGCCGGCTCCCCGGGCTCCCCCACGTGCTGCCGC
                  ************************************************************

HsCD00515631      TGCAAGGAGCTGGGGCTGGAGAAGGAGGATGCGGCGCTGTTGCCCCGCGCGGGGTTGGAC
NM_198508.2       TGCAAGGAGCTGGGGCTGGAGAAGGAGGATGCGGCGCTGTTGCCCCGCGCGGGGTTGGAC
                  ************************************************************

HsCD00515631      GGCGACGAGAAGCTGCCCCGGGCCGTAACGCTTACGGGGCTACCCATGTACGTGAAGTCC
NM_198508.2       GGCGACGAGAAGCTGCCCCGGGCCGTAACGCTTACGGGGCTACCCATGTACGTGAAGTCC
                  ************************************************************

HsCD00515631      CTGTACTGGGCCCTGGCGTTCATGGCTGTGCTCCTGGCAGTCTCTGGGGTTGTCATTGTG
NM_198508.2       CTGTACTGGGCCCTGGCGTTCATGGCTGTGCTCCTGGCAGTCTCTGGGGTTGTCATTGTG
                  ************************************************************

HsCD00515631      GTCCTGGCCTCAAGAGCAGGAGCCAGATGCCAGCAGTGCCCCCCAGGCTGGGTGTTGTCC
NM_198508.2       GTCCTGGCCTCAAGAGCAGGAGCCAGATGCCAGCAGTGCCCCCCAGGCTGGGTGTTGTCC
                  ************************************************************

HsCD00515631      GAGGAGCACTGTTACTACTTCTCTGCAGAAGCGCAGGCCTGGGAAGCCAGCCAGGCTTTC
NM_198508.2       GAGGAGCACTGTTACTACTTCTCTGCAGAAGCGCAGGCCTGGGAAGCCAGCCAGGCTTTC
                  ************************************************************

HsCD00515631      TGCTCAGCCTACCACGCTACCCTCCCCCTGCTAAGCCACACCCAGGACTTCCTGGGCAGA
NM_198508.2       TGCTCAGCCTACCACGCTACCCTCCCCCTGCTAAGCCACACCCAGGACTTCCTGGGCAGA
                  ************************************************************

HsCD00515631      TACCCAGTCTCCAGGCACTCCTGGGTGGGGGCCTGGCGAGGCCCCCAGGGCTGGCACTGG
NM_198508.2       TACCCAGTCTCCAGGCACTCCTGGGTGGGGGCCTGGCGAGGCCCCCAGGGCTGGCACTGG
                  ************************************************************

HsCD00515631      ATCGACGAGGCCCCACTCCCGCCCCAGCTACTCCCTGAGGACGGCGAGGACAATCTGGAT
NM_198508.2       ATCGACGAGGCCCCACTCCCGCCCCAGCTACTCCCTGAGGACGGCGAGGACAATCTGGAT
                  ************************************************************

HsCD00515631      ATCAACTGTGGGGCCCTGGAGGAAGGCACGCTGGTGGCTGCAAACTGCAGCACTCCAAGA
NM_198508.2       ATCAACTGTGGGGCCCTGGAGGAAGGCACGCTGGTGGCTGCAAACTGCAGCACTCCAAGA
                  ************************************************************

HsCD00515631      CCCTGGGTCTGTGCCAAGGGGACCCAG---
NM_198508.2       CCCTGGGTCTGTGCCAAGGGGACCCAGTGA
                  ***************************