DNASU Plasmid | OMD (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: OMD

Gene Name: osteomodulin

Original Clone ID:

ccsbBroadEn_01115

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC046356.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515656

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1264nts

Open reading frame: 1 to 1264

Reference Sequence Alignment

ATGGGTTTTTTAAGTCCAATATATGTTATTTTCTTCTTTTTTGGAGTCAAAGTACATTGC
CAATATGAAACTTATCAGTGGGATGAAGACTATGACCAAGAGCCAGATGATGATTACCAA
ACAGGATTCCCATTTCGTCAAAATGTAGACTACGGAGTTCCTTTTCATCAGTATACTTTA
GGCTGTGTCAGTGAATGCTTCTGTCCAACTAACTTTCCATCATCAATGTACTGTGATAAT
CGCAAACTCAAGACTATCCCAAATATTCCGATGCACATTCAGCAACTCTACCTTCAGTTC
AATGAAATTGAGGCTGTGACTGCAAATTCATTCATCAATGCAACTCATCTTAAAGAAATT
AACCTCAGCCACAACAAAATTAAATCTCAAAAGATTGATTATGGTGTGTTTGCTAAGCTT
CCAAATCTACTACAACTTCATCTAGAGCATAATAATTTAGAAGAATTTCCATTTCCTCTT
CCTAAATCTCTGGAAAGACTCCTTCTTGGTTACAATGAAATCTCCAAACTGCAGACAAAT
GCTATGGATGGGCTAGTAAACTTGACCATGCTTGATCTCTGTTATAATTATCTTCATGAT
TCTCTGCTAAAAGACAAAATCTTTGCCAAAATGGAAAAACTAATGCAGCTCAACCTCTGC
AGTAACAGATTAGAATCAATGCCTCCTGGTTTGCCTTCTTCACTTATGTATCTGTCTTTA
GAAAATAATTCAATTTCTTCTATACCCGAAAAATACTTCGACAAACTTCCAAAACTTCAT
ACTCTAAGAATGTCACACAACAAACTACAAGACATCCCATATAATATTTTTAATCTTCCC
AACATTGTAGAACTCAGTGTTGGACACAACAAATTGAAGCAAGCATTCTATATTCCAAGA
AATTTGGAACACCTATACCTACAAAATAATGAAATAGAAAAGATGAATCTTACAGTGATG
TGTCCTTCTATTGACCCACTACATTACCACCATTTAACATACATTCGTGTGGACCAAAAT
AAACTAAAAGAACCAATAAGCTCATACATCTTCTTCTGCTTCCCTCATATACACACTATT
TATTATGGTGAACAACGAAGCACTAATGGTCAAACAATACAACTAAAGACACAAGTTTTC
AGGAGATTTCCAGATGATGATGATGAAAGTGAAGATCACGATGATCCTGACAATGCTCAT
GAGAGCCCAGAACAAGAAGGAGCAGAAGGGCACTTTGACCTTCATTATTATGAAAATCAA
GAA

Protein sequence predicted based on nucleotide sequence

MGFLSPIYVIFFFFGVKVHCQYETYQWDEDYDQEPDDDYQTGFPFRQNVDYGVPFHQYTL
GCVSECFCPTNFPSSMYCDNRKLKTIPNIPMHIQQLYLQFNEIEAVTANSFINATHLKEI
NLSHNKIKSQKIDYGVFAKLPNLLQLHLEHNNLEEFPFPLPKSLERLLLGYNEISKLQTN
AMDGLVNLTMLDLCYNYLHDSLLKDKIFAKMEKLMQLNLCSNRLESMPPGLPSSLMYLSL
ENNSISSIPEKYFDKLPKLHTLRMSHNKLQDIPYNIFNLPNIVELSVGHNKLKQAFYIPR
NLEHLYLQNNEIEKMNLTVMCPSIDPLHYHHLTYIRVDQNKLKEPISSYIFFCFPHIHTI
YYGEQRSTNGQTIQLKTQVFRRFPDDDDESEDHDDPDNAHESPEQEGAEGHFDLHYYENQ
E

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

OMD

Symbol Nomenclature :

OMD

SYNONYM :

OSAD; SLRR2C

Designation :

KSPG osteomodulin|keratan sulfate proteoglycan osteomodulin|osteoadherin proteoglycan

Full Nomenclature :

osteomodulin

GENEID :

4958

Locus Tag :

UNQ190/PRO216

HGNC :

8134

Vega :

OTTHUMG00000020225

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515656      ATGGGTTTTTTAAGTCCAATATATGTTATTTTCTTCTTTTTTGGAGTCAAAGTACATTGC
NM_005014.2       ATGGGTTTTTTAAGTCCAATATATGTTATTTTCTTCTTTTTTGGAGTCAAAGTACATTGC
                  ************************************************************

HsCD00515656      CAATATGAAACTTATCAGTGGGATGAAGACTATGACCAAGAGCCAGATGATGATTACCAA
NM_005014.2       CAATATGAAACTTATCAGTGGGATGAAGACTATGACCAAGAGCCAGATGATGATTACCAA
                  ************************************************************

HsCD00515656      ACAGGATTCCCATTTCGTCAAAATGTAGACTACGGAGTTCCTTTTCATCAGTATACTTTA
NM_005014.2       ACAGGATTCCCATTTCGTCAAAATGTAGACTACGGAGTTCCTTTTCATCAGTATACTTTA
                  ************************************************************

HsCD00515656      GGCTGTGTCAGTGAATGCTTCTGTCCAACTAACTTTCCATCATCAATGTACTGTGATAAT
NM_005014.2       GGCTGTGTCAGTGAATGCTTCTGTCCAACTAACTTTCCATCATCAATGTACTGTGATAAT
                  ************************************************************

HsCD00515656      CGCAAACTCAAGACTATCCCAAATATTCCGATGCACATTCAGCAACTCTACCTTCAGTTC
NM_005014.2       CGCAAACTCAAGACTATCCCAAATATTCCGATGCACATTCAGCAACTCTACCTTCAGTTC
                  ************************************************************

HsCD00515656      AATGAAATTGAGGCTGTGACTGCAAATTCATTCATCAATGCAACTCATCTTAAAGAAATT
NM_005014.2       AATGAAATTGAGGCTGTGACTGCAAATTCATTCATCAATGCAACTCATCTTAAAGAAATT
                  ************************************************************

HsCD00515656      AACCTCAGCCACAACAAAATTAAATCTCAAAAGATTGATTATGGTGTGTTTGCTAAGCTT
NM_005014.2       AACCTCAGCCACAACAAAATTAAATCTCAAAAGATTGATTATGGTGTGTTTGCTAAGCTT
                  ************************************************************

HsCD00515656      CCAAATCTACTACAACTTCATCTAGAGCATAATAATTTAGAAGAATTTCCATTTCCTCTT
NM_005014.2       CCAAATCTACTACAACTTCATCTAGAGCATAATAATTTAGAAGAATTTCCATTTCCTCTT
                  ************************************************************

HsCD00515656      CCTAAATCTCTGGAAAGACTCCTTCTTGGTTACAATGAAATCTCCAAACTGCAGACAAAT
NM_005014.2       CCTAAATCTCTGGAAAGACTCCTTCTTGGTTACAATGAAATCTCCAAACTGCAGACAAAT
                  ************************************************************

HsCD00515656      GCTATGGATGGGCTAGTAAACTTGACCATGCTTGATCTCTGTTATAATTATCTTCATGAT
NM_005014.2       GCTATGGATGGGCTAGTAAACTTGACCATGCTTGATCTCTGTTATAATTATCTTCATGAT
                  ************************************************************

HsCD00515656      TCTCTGCTAAAAGACAAAATCTTTGCCAAAATGGAAAAACTAATGCAGCTCAACCTCTGC
NM_005014.2       TCTCTGCTAAAAGACAAAATCTTTGCCAAAATGGAAAAACTAATGCAGCTCAACCTCTGC
                  ************************************************************

HsCD00515656      AGTAACAGATTAGAATCAATGCCTCCTGGTTTGCCTTCTTCACTTATGTATCTGTCTTTA
NM_005014.2       AGTAACAGATTAGAATCAATGCCTCCTGGTTTGCCTTCTTCACTTATGTATCTGTCTTTA
                  ************************************************************

HsCD00515656      GAAAATAATTCAATTTCTTCTATACCCGAAAAATACTTCGACAAACTTCCAAAACTTCAT
NM_005014.2       GAAAATAATTCAATTTCTTCTATACCCGAAAAATACTTCGACAAACTTCCAAAACTTCAT
                  ************************************************************

HsCD00515656      ACTCTAAGAATGTCACACAACAAACTACAAGACATCCCATATAATATTTTTAATCTTCCC
NM_005014.2       ACTCTAAGAATGTCACACAACAAACTACAAGACATCCCATATAATATTTTTAATCTTCCC
                  ************************************************************

HsCD00515656      AACATTGTAGAACTCAGTGTTGGACACAACAAATTGAAGCAAGCATTCTATATTCCAAGA
NM_005014.2       AACATTGTAGAACTCAGTGTTGGACACAACAAATTGAAGCAAGCATTCTATATTCCAAGA
                  ************************************************************

HsCD00515656      AATTTGGAACACCTATACCTACAAAATAATGAAATAGAAAAGATGAATCTTACAGTGATG
NM_005014.2       AATTTGGAACACCTATACCTACAAAATAATGAAATAGAAAAGATGAATCTTACAGTGATG
                  ************************************************************

HsCD00515656      TGTCCTTCTATTGACCCACTACATTACCACCATTTAACATACATTCGTGTGGACCAAAAT
NM_005014.2       TGTCCTTCTATTGACCCACTACATTACCACCATTTAACATACATTCGTGTGGACCAAAAT
                  ************************************************************

HsCD00515656      AAACTAAAAGAACCAATAAGCTCATACATCTTCTTCTGCTTCCCTCATATACACACTATT
NM_005014.2       AAACTAAAAGAACCAATAAGCTCATACATCTTCTTCTGCTTCCCTCATATACACACTATT
                  ************************************************************

HsCD00515656      TATTATGGTGAACAACGAAGCACTAATGGTCAAACAATACAACTAAAGACACAAGTTTTC
NM_005014.2       TATTATGGTGAACAACGAAGCACTAATGGTCAAACAATACAACTAAAGACACAAGTTTTC
                  ************************************************************

HsCD00515656      AGGAGATTTCCAGATGATGATGATGAAAGTGAAGATCACGATGATCCTGACAATGCTCAT
NM_005014.2       AGGAGATTTCCAGATGATGATGATGAAAGTGAAGATCACGATGATCCTGACAATGCTCAT
                  ************************************************************

HsCD00515656      GAGAGCCCAGAACAAGAAGGAGCAGAAGGGCACTTTGACCTTCATTATTATGAAAATCAA
NM_005014.2       GAGAGCCCAGAACAAGAAGGAGCAGAAGGGCACTTTGACCTTCATTATTATGAAAATCAA
                  ************************************************************

HsCD00515656      GAA---
NM_005014.2       GAATAG
                  ***

Pathways

Reactome :

Disease

Reactome :

Glycogen storage diseases

Reactome :

Glycosaminoglycan metabolism

Reactome :

Keratan sulfate biosynthesis

Reactome :

Keratan sulfate degradation

Reactome :

Keratan sulfate/keratin metabolism

Reactome :

Metabolism

Reactome :

Metabolism of carbohydrates

Reactome :

MPS I - Hurler syndrome

Reactome :

MPS II - Hunter syndrome

Reactome :

MPS IIIA - Sanfilippo syndrome A

Reactome :

MPS IIIB - Sanfilippo syndrome B

Reactome :

MPS IIIC - Sanfilippo syndrome C

Reactome :

MPS IIID - Sanfilippo syndrome D

Reactome :

MPS IV - Morquio syndrome A

Reactome :

MPS IV - Morquio syndrome B

Reactome :

MPS IX - Natowicz syndrome

Reactome :

MPS VI - Maroteaux-Lamy syndrome

Reactome :

MPS VII - Sly syndrome

Reactome :

Mucopolysaccharidoses

Reactome :

Myoclonic epilepsy of Lafora

Protein

SMART domain :

LRR_TYP : Leucine-rich repeats, typical (most populated) subfamily

SMART domain :

LRRNT : Leucine rich repeat N-terminal domain

HPRD :

10128

Clone

Cloning Information :

669363

Original Clone ID :

ccsbBroadEn_01115

Organism

Species Specific ID:

4958

Genome

Chromosome :

Map Location :

9q22.31

Ensembl :

ENSG00000127083

Reagents

Labome :

osteoadherin-antibody