DNASU Plasmid | ENOSF1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ENOSF1

Gene Name: enolase superfamily member 1

Original Clone ID:

ccsbBroadEn_03604

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001285.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515686

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1330nts

Open reading frame: 1 to 1330

Reference Sequence Alignment

ATGGTGCGCGGCAGGATCTCCCGGCTCTCGGTCCGGGACGTGCGCTTCCCCACGTCGCTT
GGGGGCCACGGCGCGGACGCCATGCACACGGACCCTGACTACTCGGCTGCCTATGTCGTC
ATAGAAACTGATGCAGAAGATGGAATCAAGGGGTGTGGAATTACCTTCACTCTGGGAAAA
GGCACTGAAGTTGTTGTCTGTGCTGTGAATGCCCTCGCCCACCATGTGCTCAACAAGGAC
CTCAAGGACATTGTTGGTGACTTCAGAGGCTTCTATAGGCAGCTCACAAGTGATGGGCAG
CTCAGATGGATTGGTCCAGAAAAGGGCGTGGTGCACCTGGCGACAGCGGCCGTCCTAAAC
GCGGTGTGGGACTTGTGGGCCAAGCAGGAGGGAAAGCCTGTCTGGAAGTTACTTGTGGAC
ATGGATCCCAGGATGCTGGTATCCTGCATAGATTTCAGGTACATCACTGATGTCCTGACT
GAGGAGGATGCCCTAGAAATACTGCAGAAAGGTCAAATTGGTAAAAAAGAAAGAGAGAAG
CAAATGCTGGCACAAGGATACCCTGCTTACACGACATCGTGCGCCTGGCTGGGGTACTCA
GATGACACGTTGAAGCAGCTCTGTGCCCAGGCGCTGAAGGATGGCTGGACCAGGTTTAAA
GTAAAGGTGGGTGCTGATCTCCAGGATGACATGCGAAGATGCCAAATCATCCGAGACATG
ATTGGACCGGAAAAGACTTTGATGATGGATGCCAACCAGCGCTGGGATGTGCCTGAGGCG
GTGGAGTGGATGTCCAAGCTGGCCAAGTTCAAGCCATTGTGGATTGAGGAGCCAACCTCC
CCTGATGACATTCTGGGGCACGCCACCATTTCCAAGGCACTGGTCCCATTAGGAATTGGC
ATTGCCACAGGAGAACAGTGCCACAATAGAGTGATATTTAAGCAACTCCTACAGGCGAAG
GCCCTGCAGTTCCTCCAGATTGACAGTTGCAGACTGGGCAGTGTCAATGAGAACCTCTCA
GTATTGCTGATGGCCAAAAAGTTTGAAATTCCTGTTTGCCCCCATGCTGGTGGAGTTGGC
CTCTGTGAACTGGTGCAGCACCTGATTATATTTGACTACATATCAGTTTCTGCAAGCCTT
GAAAATAGGGTGTGTGAGTATGTTGACCACCTGCATGAGCATTTCAAGTATCCCGTGATG
ATCCAGCGGGCTTCCTACATGCCTCCCAAGGATCCCGGCTACTCAACAGAAATGAAGGAG
GAATCTGTAAAGAAACACCAGTATCCAGATGGTGAAGTTTGGAAGAAACTCCTTCCTGCT
CAAGAAAAT

Protein sequence predicted based on nucleotide sequence

MVRGRISRLSVRDVRFPTSLGGHGADAMHTDPDYSAAYVVIETDAEDGIKGCGITFTLGK
GTEVVVCAVNALAHHVLNKDLKDIVGDFRGFYRQLTSDGQLRWIGPEKGVVHLATAAVLN
AVWDLWAKQEGKPVWKLLVDMDPRMLVSCIDFRYITDVLTEEDALEILQKGQIGKKEREK
QMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALKDGWTRFKVKVGADLQDDMRRCQIIRDM
IGPEKTLMMDANQRWDVPEAVEWMSKLAKFKPLWIEEPTSPDDILGHATISKALVPLGIG
IATGEQCHNRVIFKQLLQAKALQFLQIDSCRLGSVNENLSVLLMAKKFEIPVCPHAGGVG
LCELVQHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASYMPPKDPGYSTEMKE
ESVKKHQYPDGEVWKKLLPAQEN

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ENOSF1

Symbol Nomenclature :

ENOSF1

SYNONYM :

HSRTSBETA; RTS; TYMSAS

Designation :

antisense RNA to thymidylate synthase|mitochondrial enolase superfamily member 1

Full Nomenclature :

enolase superfamily member 1

GENEID :

55556

HGNC :

30365

MIM :

607427

Vega :

OTTHUMG00000131470

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515686      ------------------------------------------------------------
NM_017512.5       ------------------------------------------------------------
NM_202758.3       ATGGTAAGCGCGGACGCCATGGTAAGCGCGGACGCCATGGTAAGCGCGGACGCCATGGTA
                                                                              

HsCD00515686      ---ATGGTGCGCGGCAGGATCTCCCGGCTCTCGGTCCGGGACGTGCGCTTCCCCACGTCG
NM_017512.5       ---ATGGTGCGCGGCAGGATCTCCCGGCTCTCGGTCCGGGACGTGCGCTTCCCCACGTCG
NM_202758.3       AGCGCGGACGCCATGGTAAGCGCGGACGCCATGGTAAGCGCGGACGCCATGGTAAGCGCG
                     . **:   *.  . .* * *  .   *: ***..* *. *:   *:*   .*   **

HsCD00515686      CTTGGGGGCCACGGCGCGGACGCCATGCACACGGACCCTGACTACTCGGCTGCCTATGTC
NM_017512.5       CTTGGGGGCCACGGCGCGGACGCCATGCACACGGACCCTGACTACTCGGCTGCCTATGTC
NM_202758.3       GACGCCATGGTAAGCGCGGACGCCATGCACACGGACCCTGACTACTCGGCTGCCTATGTC
                   : *  .   :..***********************************************

HsCD00515686      GTCATAGAAACTGATGCAGAAGATGGAATCAAGGGGTGTGGAATTACCTTCACTCTGGGA
NM_017512.5       GTCATAGAAACTGATGCAGAAGATGGAATCAAGGGGTGTGGAATTACCTTCACTCTGGGA
NM_202758.3       GTCATAGAAACTGATGCAGAAGATGGAATCAAGGGGTGTGGAATTACCTTCACTCTGGGA
                  ************************************************************

HsCD00515686      AAAGGCACTGAAGTTGTTGTCTGTGCTGTGAATGCCCTCGCCCACCATGTGCTCAACAAG
NM_017512.5       AAAGGCACTGAAGTTGTTGTCTGTGCTGTGAATGCCCTCGCCCACCATGTGCTCAACAAG
NM_202758.3       AAAGGCACTGAAGTTGTTGTCTGTGCTGTGAATGCCCTCGCCCACCATGTGCTCAACAAG
                  ************************************************************

HsCD00515686      GACCTCAAGGACATTGTTGGTGACTTCAGAGGCTTCTATAGGCAGCTCACAAGTGATGGG
NM_017512.5       GACCTCAAGGACATTGTTGGTGACTTCAGAGGCTTCTATAGGCAGCTCACAAGTGATGGG
NM_202758.3       GACCTCAAGGACATTGTTGGTGACTTCAGAGGCTTCTATAGGCAGCTCACAAGTGATGGG
                  ************************************************************

HsCD00515686      CAGCTCAGATGGATTGGTCCAGAAAAGGGCGTGGTGCACCTGGCGACAGCGGCCGTCCTA
NM_017512.5       CAGCTCAGATGGATTGGTCCAGAAAAGGGCGTGGTGCACCTGGCGACAGCGGCCGTCCTA
NM_202758.3       CAGCTCAGATGGATTGGTCCAGAAAAGGGCGTGGTGCACCTGGCGACAGCGGCCGTCCTA
                  ************************************************************

HsCD00515686      AACGCGGTGTGGGACTTGTGGGCCAAGCAGGAGGGAAAGCCTGTCTGGAAGTTACTTGTG
NM_017512.5       AACGCGGTGTGGGACTTGTGGGCCAAGCAGGAGGGAAAGCCTGTCTGGAAGTTACTTGTG
NM_202758.3       AACGCGGTGTGGGACTTGTGGGCCAAGCAGGAGGGAAAGCCTGTCTGGAAGTTACTTGTG
                  ************************************************************

HsCD00515686      GACATGGATCCCAGGATGCTGGTATCCTGCATAGATTTCAGGTACATCACTGATGTCCTG
NM_017512.5       GACATGGATCCCAGGATGCTGGTATCCTGCATAGATTTCAGGTACATCACTGATGTCCTG
NM_202758.3       GACATGGATCCCAGGATGCTGGTATCCTGCATAGATTTCAGGTACATCACTGATGTCCTG
                  ************************************************************

HsCD00515686      ACTGAGGAGGATGCCCTAGAAATACTGCAGAAAGGTCAAATTGGTAAAAAAGAAAGAGAG
NM_017512.5       ACTGAGGAGGATGCCCTAGAAATACTGCAGAAAGGTCAAATTGGTAAAAAAGAAAGAGAG
NM_202758.3       ACTGAGGAGGATGCCCTAGAAATACTGCAGAAAGGTCAAATTGGTAAAAAAGAAAGAGAG
                  ************************************************************

HsCD00515686      AAGCAAATGCTGGCACAAGGATACCCTGCTTACACGACATCGTGCGCCTGGCTGGGGTAC
NM_017512.5       AAGCAAATGCTGGCACAAGGATACCCTGCTTACACGACATCGTGCGCCTGGCTGGGGTAC
NM_202758.3       AAGCAAATGCTGGCACAAGGATACCCTGCTTACACGACATCGTGCGCCTGGCTGGGGTAC
                  ************************************************************

HsCD00515686      TCAGATGACACGTTGAAGCAGCTCTGTGCCCAGGCGCTGAAGGATGGCTGGACCAGGTTT
NM_017512.5       TCAGATGACACGTTGAAGCAGCTCTGTGCCCAGGCGCTGAAGGATGGCTGGACCAGGTTT
NM_202758.3       TCAGATGACACGTTGAAGCAGCTCTGTGCCCAGGCGCTGAAGGATGGCTGGACCAGGTTT
                  ************************************************************

HsCD00515686      AAAGTAAAGGTGGGTGCTGATCTCCAGGATGACATGCGAAGATGCCAAATCATCCGAGAC
NM_017512.5       AAAGTAAAGGTGGGTGCTGATCTCCAGGATGACATGCGAAGATGCCAAATCATCCGAGAC
NM_202758.3       AAAGTAAAGGTGGGTGCTGATCTCCAGGATGACATGCGAAGATGCCAAATCATCCGAGAC
                  ************************************************************

HsCD00515686      ATGATTGGACCGGAAAAGACTTTGATGATGGATGCCAACCAGCGCTGGGATGTGCCTGAG
NM_017512.5       ATGATTGGACCGGAAAAGACTTTGATGATGGATGCCAACCAGCGCTGGGATGTGCCTGAG
NM_202758.3       ATGATTGGACCGGAAAAGACTTTGATGATGGATGCCAACCAGCGCTGGGATGTGCCTGAG
                  ************************************************************

HsCD00515686      GCGGTGGAGTGGATGTCCAAGCTGGCCAAGTTCAAGCCATTGTGGATTGAGGAGCCAACC
NM_017512.5       GCGGTGGAGTGGATGTCCAAGCTGGCCAAGTTCAAGCCATTGTGGATTGAGGAGCCAACC
NM_202758.3       GCGGTGGAGTGGATGTCCAAGCTGGCCAAGTTCAAGCCATTGTGGATTGAGGAGCCAACC
                  ************************************************************

HsCD00515686      TCCCCTGATGACATTCTGGGGCACGCCACCATTTCCAAGGCACTGGTCCCATTAGGAATT
NM_017512.5       TCCCCTGATGACATTCTGGGGCACGCCACCATTTCCAAGGCACTGGTCCCATTAGGAATT
NM_202758.3       TCCCCTGATGACATTCTGGGGCACGCCACCATTTCCAAG---------------------
                  ***************************************                     

HsCD00515686      GGCATTGCCACAGGAGAACAGTGCCACAATAGAGTGATATTTAAGCAACTCCTACAGGCG
NM_017512.5       GGCATTGCCACAGGAGAACAGTGCCACAATAGAGTGATATTTAAGCAACTCCTACAGGCG
NM_202758.3       ---------------------TGCCACAATAGAGTGATATTTAAGCAACTCCTACAGGCG
                                       ***************************************

HsCD00515686      AAGGCCCTGCAGTTCCTCCAGATTGACAGTTGCAGACTGGGCAGTGTCAATGAGAACCTC
NM_017512.5       AAGGCCCTGCAGTTCCTCCAGATTGACAGTTGCAGACTGGGCAGTGTCAATGAGAACCTC
NM_202758.3       AAGGCCCTGCAGTTCCTCCAGATTGACAGTTGCAGACTGGGCAGTGTCAATGAGAACCTC
                  ************************************************************

HsCD00515686      TCAGTATTGCTGATGGCCAAAAAGTTTGAAATTCCTGTTTGCCCCCATGCTGGTGGAGTT
NM_017512.5       TCAGTATTGCTGATGGCCAAAAAGTTTGAAATTCCTGTTTGCCCCCATGCTGGTGGAGTT
NM_202758.3       TCAGTATTGCTGATGGCCAAAAAGTTTGAAATTCCTGTTTGCCCCCATGCTGGTGGAGTT
                  ************************************************************

HsCD00515686      GGCCTCTGTGAACTGGTGCAGCACCTGATTATATTTGACTACATATCAGTTTCTGCAAGC
NM_017512.5       GGCCTCTGTGAACTGGTGCAGCACCTGATTATATTTGACTACATATCAGTTTCTGCAAGC
NM_202758.3       GGCCTCTGTGAACTGGTGCAGCACCTGATTATATTTGACTACATATCAGTTTCTGCAAGC
                  ************************************************************

HsCD00515686      CTTGAAAATAGGGTGTGTGAGTATGTTGACCACCTGCATGAGCATTTCAAGTATCCCGTG
NM_017512.5       CTTGAAAATAGGGTGTGTGAGTATGTTGACCACCTGCATGAGCATTTCAAGTATCCCGTG
NM_202758.3       CTTGAAAATAGGGTGTGTGAGTATGTTGACCACCTGCATGAGCATTTCAAGTATCCCGTG
                  ************************************************************

HsCD00515686      ATGATCCAGCGGGCTTCCTACATGCCTCCCAAGGATCCCGGCTACTCAACAGAAATGAAG
NM_017512.5       ATGATCCAGCGGGCTTCCTACATGCCTCCCAAGGATCCCGGCTACTCAACAGAAATGAAG
NM_202758.3       ATGATCCAGCGGGCTTCCTACATGCCTCCCAAGGATCCCGGCTACTCAACAGAAATGAAG
                  ************************************************************

HsCD00515686      GAGGAATCTGTAAAGAAACACCAGTATCCAGATGGTGAAGTTTGGAAGAAACTCCTTCCT
NM_017512.5       GAGGAATCTGTAAAGAAACACCAGTATCCAGATGGTGAAGTTTGGAAGAAACTCCTTCCT
NM_202758.3       GAGGAATCTGTAAAGAAACACCAGTATCCAGATGGTGAAGTTTGGAAGAAACTCCTTCCT
                  ************************************************************

HsCD00515686      GCTCAAGAAAAT---
NM_017512.5       GCTCAAGAAAATTAA
NM_202758.3       GCTCAAGAAAATTAA
                  ************