DNASU Plasmid | SPRED1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SPRED1

Gene Name: sprouty-related, EVH1 domain containing 1

Original Clone ID:

ccsbBroadEn_09737

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC137480.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515688

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1333nts

Open reading frame: 1 to 1333

Reference Sequence Alignment

ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTG
GTGATGACCCGAGATGACTCAAGTGGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGC
AGCGTCACTGTCTTCAAAGTCCCTCATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATC
CGTGGAGAGCGACTCAGGGACAAAATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTC
ATTTATAATAAGGTCACTCCAACATTTCACCACTGGAAGATTGATGACAAAAAGTTTGGT
CTTACGTTTCAAAGTCCTGCTGATGCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATA
GAGGATATTTCTCAAGGATGCCCCGAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTA
CAAGCAAATGAAGAGGATTCTTCCAGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAG
ACAGTTGTTACCAGTGAGCCTTATAGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGAT
CTGAATGCCAGAAGAGTCTACATGCAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCA
GGCTTGGACATTCAGAGCAGAAGTATGGAATACGTACAGCGGCAAATATCCAAGGAATGT
GGAAGCCTAAAGTCCCAAAATAGGGTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAA
GATGAGGATGAGATTGTCAGAATAAACCCTCGAGATATCTTAATACGTCGCTATGCAGAC
TACAGACATCCTGACATGTGGAAAAATGACTTGGAAAGAGATGATGCTGATTCCAGTATT
CAGTTTTCTAAACCAGACAGTAAAAAATCAGACTATCTGTACTCTTGTGGGGATGAGACT
AAGTTAAGTTCACCCAAAGACTCTGTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATT
AAGAAGTCAAAACGAAGAAAAGAGGATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAA
AGGTTTAATCATGAAGAAAATGTCAGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAA
AGATGCATATATCAAGTTAGTTGCATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATG
TCAGACTCAGAGGGAGATTTTTCTGATCCCTGTTCGTGTGACACTAGCGACGACAAGTTC
TGCTTGCGATGGTTAGCCCTGGTAGCTTTGTCTTTCATTGTACCATGTATGTGCTGCTAC
GTCCCTTTGAGAATGTGCCATCGCTGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACAT
AAAGCTGCTGGA

Protein sequence predicted based on nucleotide sequence

MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFI
RGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAI
EDISQGCPESKNEAEGADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFED
LNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQ
DEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDET
KLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIK
RCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCY
VPLRMCHRCGEACGCCGGKHKAAG

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SPRED1

Symbol Nomenclature :

SPRED1

SYNONYM :

NFLS; hSpred1; spred-1

Designation :

sprouty-related, EVH1 domain-containing protein 1|suppressor of Ras/MAPK activation

Full Nomenclature :

sprouty-related, EVH1 domain containing 1

GENEID :

161742

HGNC :

20249

MIM :

609291

Vega :

OTTHUMG00000172377

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515688        ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAG----------------------
NM_152594.2         ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAG----------------------
XM_005254202.1      ATGAGCGAGGAGACGGCGACTTCTGACAACGAGTGTCAGGGGCATGATGATGGAATCTGC
                    ******************************** :.*..                      

HsCD00515688        --------------TTATGCACGAGTGCGAGCTGTGGTGATGACCCGAGATGACTCAAGT
NM_152594.2         --------------TTATGCACGAGTGCGAGCTGTGGTGATGACCCGAGATGACTCAAGT
XM_005254202.1      TTTTTTCATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCCGAGATGACTCAAGT
                                  **********************************************

HsCD00515688        GGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCT
NM_152594.2         GGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCT
XM_005254202.1      GGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGTCCCT
                    ************************************************************

HsCD00515688        CATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAA
NM_152594.2         CATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAA
XM_005254202.1      CATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAA
                    ************************************************************

HsCD00515688        ATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACA
NM_152594.2         ATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACA
XM_005254202.1      ATGGTGGTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACA
                    ************************************************************

HsCD00515688        TTTCACCACTGGAAGATTGATGACAAAAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGAT
NM_152594.2         TTTCACCACTGGAAGATTGATGACAAGAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGAT
XM_005254202.1      TTTCACCACTGGAAGATTGATGACAAGAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGAT
                    **************************.*********************************

HsCD00515688        GCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATAGAGGATATTTCTCAAGGATGCCCC
NM_152594.2         GCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATAGAGGATATTTCTCAAGGATGCCCC
XM_005254202.1      GCTAGGGCTTTTGATAGAGGTATCCGAAGAGCTATAGAGGATATTTCTCAAGGATGCCCC
                    ************************************************************

HsCD00515688        GAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTACAAGCAAATGAAGAGGATTCTTCC
NM_152594.2         GAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTACAAGCAAATGAAGAGGATTCTTCC
XM_005254202.1      GAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTACAAGCAAATGAAGAGGATTCTTCC
                    ************************************************************

HsCD00515688        AGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTTACCAGTGAGCCTTAT
NM_152594.2         AGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTTACCAGTGAGCCTTAT
XM_005254202.1      AGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTTACCAGTGAGCCTTAT
                    ************************************************************

HsCD00515688        AGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCCAGAAGAGTCTACATG
NM_152594.2         AGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCCAGAAGAGTCTACATG
XM_005254202.1      AGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCCAGAAGAGTCTACATG
                    ************************************************************

HsCD00515688        CAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGACATTCAGAGCAGAAGT
NM_152594.2         CAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGACATTCAGAGCAGAAGT
XM_005254202.1      CAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGACATTCAGAGCAGAAGT
                    ************************************************************

HsCD00515688        ATGGAATACGTACAGCGGCAAATATCCAAGGAATGTGGAAGCCTAAAGTCCCAAAATAGG
NM_152594.2         ATGGAATACGTACAGCGGCAAATATCCAAGGAATGTGGAAGCCTAAAGTCCCAAAATAGG
XM_005254202.1      ATGGAATACGTACAGCGGCAAATATCCAAGGAATGTGGAAGCCTAAAGTCCCAAAATAGG
                    ************************************************************

HsCD00515688        GTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTCAGAATA
NM_152594.2         GTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTCAGAATA
XM_005254202.1      GTCCCTTTGAAATCAATCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTCAGAATA
                    ************************************************************

HsCD00515688        AACCCTCGAGATATCTTAATACGTCGCTATGCAGACTACAGACATCCTGACATGTGGAAA
NM_152594.2         AACCCTCGAGATATCTTAATACGTCGCTATGCAGACTACAGACATCCTGACATGTGGAAA
XM_005254202.1      AACCCTCGAGATATCTTAATACGTCGCTATGCAGACTACAGACATCCTGACATGTGGAAA
                    ************************************************************

HsCD00515688        AATGACTTGGAAAGAGATGATGCTGATTCCAGTATTCAGTTTTCTAAACCAGACAGTAAA
NM_152594.2         AATGACTTGGAAAGAGATGATGCTGATTCCAGTATTCAGTTTTCTAAACCAGACAGTAAA
XM_005254202.1      AATGACTTGGAAAGAGATGATGCTGATTCCAGTATTCAGTTTTCTAAACCAGACAGTAAA
                    ************************************************************

HsCD00515688        AAATCAGACTATCTGTACTCTTGTGGGGATGAGACTAAGTTAAGTTCACCCAAAGACTCT
NM_152594.2         AAATCAGACTATCTGTACTCTTGTGGGGATGAGACTAAGTTAAGTTCACCCAAAGACTCT
XM_005254202.1      AAATCAGACTATCTGTACTCTTGTGGGGATGAGACTAAGTTAAGTTCACCCAAAGACTCT
                    ************************************************************

HsCD00515688        GTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATTAAGAAGTCAAAACGAAGAAAAGAG
NM_152594.2         GTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATTAAGAAGTCAAAACGAAGAAAAGAG
XM_005254202.1      GTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATTAAGAAGTCAAAACGAAGAAAAGAG
                    ************************************************************

HsCD00515688        GATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAATGTC
NM_152594.2         GATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAATGTT
XM_005254202.1      GATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAATGTT
                    *********************************************************** 

HsCD00515688        AGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAAAGATGCATATATCAAGTTAGTTGC
NM_152594.2         AGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAAAGATGCATATATCAAGTTAGTTGC
XM_005254202.1      AGGGGAAAATGTCAGGATGCTCCAGACCCTATTAAAAGATGCATATATCAAGTTAGTTGC
                    ************************************************************

HsCD00515688        ATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATGTCAGACTCAGAGGGAGATTTTTCT
NM_152594.2         ATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATGTCAGACTCAGAGGGAGATTTTTCT
XM_005254202.1      ATGCTCTGTGCAGAGAGCATGTTGTATCATTGTATGTCAGACTCAGAGGGAGATTTTTCT
                    ************************************************************

HsCD00515688        GATCCCTGTTCGTGTGACACTAGCGACGACAAGTTCTGCTTGCGATGGTTAGCCCTGGTA
NM_152594.2         GATCCCTGTTCGTGTGACACTAGCGACGACAAGTTCTGCTTGCGATGGTTAGCCCTGGTA
XM_005254202.1      GATCCCTGTTCGTGTGACACTAGCGACGACAAGTTCTGCTTGCGATGGTTAGCCCTGGTA
                    ************************************************************

HsCD00515688        GCTTTGTCTTTCATTGTACCATGTATGTGCTGCTACGTCCCTTTGAGAATGTGCCATCGC
NM_152594.2         GCTTTGTCTTTCATTGTACCATGTATGTGCTGCTACGTCCCTTTGAGAATGTGCCATCGC
XM_005254202.1      GCTTTGTCTTTCATTGTACCATGTATGTGCTGCTACGTCCCTTTGAGAATGTGCCATCGC
                    ************************************************************

HsCD00515688        TGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACATAAAGCTGCTGGA---
NM_152594.2         TGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACATAAAGCTGCTGGATGA
XM_005254202.1      TGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACATAAAGCTGCTGGATGA
                    ************************************************