DNASU Plasmid | BAG4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: BAG4

Gene Name: BCL2-associated athanogene 4

Original Clone ID:

ccsbBroadEn_07440

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC038505.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515713

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1372nts

Open reading frame: 1 to 1372

Reference Sequence Alignment

ATGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGACGGTCCGTCCTACGGCCGCTAC
TACGGGCCTGGGGGTGGAGATGTGCCGGTACACCCACCTCCACCCTTATATCCTCTTCGC
CCTGAACCTCCCCAGCCTCCCATTTCCTGGCGGGTGCGCGGGGGCGGCCCGGCGGAGACC
ACCTGGCTGGGAGAAGGCGGAGGAGGCGATGGCTACTATCCCTCGGGAGGCGCCTGGCCA
GAGCCTGGTCGAGCCGGAGGAAGCCACCAGGAGCAGCCACCATATCCTAGCTACAATTCT
AACTATTGGAATTCTACTGCGAGATCTAGGGCTCCTTACCCAAGTACATATCCTGTAAGA
CCAGAATTGCAAGGCCAGAGTTTGAATTCTTATACAAATGGAGCGTATGGTCCAACATAC
CCCCCAGGCCCTGGGGCAAATACTGCCTCATACTCAGGGGCTTATTATGCACCTGGTTAT
ACTCAGACCAGTTACTCCACAGAAGTTCCGAGTACTTACCGTTCATCTGGCAACAGCCCA
ACTCCAGTCTCTCGTTGGATCTATCCCCAGCAGGACTGTCAGACTGAAGCACCCCCTCTT
AGGGGGCAGGTTCCAGGATATCCGCCTTCACAGAACCCTGGAATGACCCTGCCCCATTAT
CCTTATGGAGATGGTAATCGTAGTGTTCCACAATCAGGACCGACTGTACGACCACAAGAA
GATGCGTGGGCTTCTCCTGGTGCTTATGGAATGGGTGGCCGTTATCCCTGGCCTTCATCA
GCGCCCTCAGCACCACCCGGCAATCTCTACATGACTGAAAGTACTTCACCATGGCCTAGC
AGTGGCTCTCCCCAGTCACCCCCTTCACCCCCAGTCCAGCAGCCCAAGGATTCTTCATAC
CCCTATAGCCAATCAGATCAAAGCATGAACCGGCACAACTTTCCTTGCAGTGTCCATCAG
TACGAATCCTCGGGGACAGTGAACAATGATGATTCAGATCTTTTGGATTCCCAAGTCCAG
TATAGTGCTGAGCCTCAGCTGTATGGTAATGCCACCAGTGACCATCCCAACAATCAAGAT
CAAAGTAGCAGTCTTCCTGAAGAATGTGTACCTTCAGATGAAAGTACTCCTCCGAGTATT
AAAAAAATCATACATGTGCTGGAGAAGGTCCAGTATCTTGAACAAGAAGTAGAAGAATTT
GTAGGAAAAAAGACAGACAAAGCATACTGGCTTCTGGAAGAAATGCTAACCAAGGAACTT
TTGGAACTGGATTCAGTTGAAACTGGGGGCCAGGACTCTGTACGGCAGGCCAGAAAAGAG
GCTGTTTGTAAGATTCAGGCCATACTGGAAAAATTAGAAAAAAAAGGATTA

Protein sequence predicted based on nucleotide sequence

MSALRRSGYGPSDGPSYGRYYGPGGGDVPVHPPPPLYPLRPEPPQPPISWRVRGGGPAET
TWLGEGGGGDGYYPSGGAWPEPGRAGGSHQEQPPYPSYNSNYWNSTARSRAPYPSTYPVR
PELQGQSLNSYTNGAYGPTYPPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSP
TPVSRWIYPQQDCQTEAPPLRGQVPGYPPSQNPGMTLPHYPYGDGNRSVPQSGPTVRPQE
DAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPSSGSPQSPPSPPVQQPKDSSY
PYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGNATSDHPNNQD
QSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKEL
LELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

BAG4

Symbol Nomenclature :

BAG4

SYNONYM :

BAG-4; SODD

Designation :

BAG family molecular chaperone regulator 4|BAG-family molecular chaperone regulator-4|bcl-2-associated athanogene 4|silencer of death domains

Full Nomenclature :

BCL2-associated athanogene 4

GENEID :

9530

HGNC :

940

MIM :

603884

Vega :

OTTHUMG00000164064

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515713      ATGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGACGGTCCGTCCTACGGCCGCTAC
NM_004874.3       ATGTCGGCCCTGAGGCGCTCGGGCTACGGCCCCAGTGACGGTCCGTCCTACGGCCGCTAC
                  ************************************************************

HsCD00515713      TACGGGCCTGGGGGTGGAGATGTGCCGGTACACCCACCTCCACCCTTATATCCTCTTCGC
NM_004874.3       TACGGGCCTGGGGGTGGAGATGTGCCGGTACACCCACCTCCACCCTTATATCCTCTTCGC
                  ************************************************************

HsCD00515713      CCTGAACCTCCCCAGCCTCCCATTTCCTGGCGGGTGCGCGGGGGCGGCCCGGCGGAGACC
NM_004874.3       CCTGAACCTCCCCAGCCTCCCATTTCCTGGCGGGTGCGCGGGGGCGGCCCGGCGGAGACC
                  ************************************************************

HsCD00515713      ACCTGGCTGGGAGAAGGCGGAGGAGGCGATGGCTACTATCCCTCGGGAGGCGCCTGGCCA
NM_004874.3       ACCTGGCTGGGAGAAGGCGGAGGAGGCGATGGCTACTATCCCTCGGGAGGCGCCTGGCCA
                  ************************************************************

HsCD00515713      GAGCCTGGTCGAGCCGGAGGAAGCCACCAGGAGCAGCCACCATATCCTAGCTACAATTCT
NM_004874.3       GAGCCTGGTCGAGCCGGAGGAAGCCACCAGGAGCAGCCACCATATCCTAGCTACAATTCT
                  ************************************************************

HsCD00515713      AACTATTGGAATTCTACTGCGAGATCTAGGGCTCCTTACCCAAGTACATATCCTGTAAGA
NM_004874.3       AACTATTGGAATTCTACTGCGAGATCTAGGGCTCCTTACCCAAGTACATATCCTGTAAGA
                  ************************************************************

HsCD00515713      CCAGAATTGCAAGGCCAGAGTTTGAATTCTTATACAAATGGAGCGTATGGTCCAACATAC
NM_004874.3       CCAGAATTGCAAGGCCAGAGTTTGAATTCTTATACAAATGGAGCGTATGGTCCAACATAC
                  ************************************************************

HsCD00515713      CCCCCAGGCCCTGGGGCAAATACTGCCTCATACTCAGGGGCTTATTATGCACCTGGTTAT
NM_004874.3       CCCCCAGGCCCTGGGGCAAATACTGCCTCATACTCAGGGGCTTATTATGCACCTGGTTAT
                  ************************************************************

HsCD00515713      ACTCAGACCAGTTACTCCACAGAAGTTCCGAGTACTTACCGTTCATCTGGCAACAGCCCA
NM_004874.3       ACTCAGACCAGTTACTCCACAGAAGTTCCAAGTACTTACCGTTCATCTGGCAACAGCCCA
                  *****************************.******************************

HsCD00515713      ACTCCAGTCTCTCGTTGGATCTATCCCCAGCAGGACTGTCAGACTGAAGCACCCCCTCTT
NM_004874.3       ACTCCAGTCTCTCGTTGGATCTATCCCCAGCAGGACTGTCAGACTGAAGCACCCCCTCTT
                  ************************************************************

HsCD00515713      AGGGGGCAGGTTCCAGGATATCCGCCTTCACAGAACCCTGGAATGACCCTGCCCCATTAT
NM_004874.3       AGGGGGCAGGTTCCAGGATATCCGCCTTCACAGAACCCTGGAATGACCCTGCCCCATTAT
                  ************************************************************

HsCD00515713      CCTTATGGAGATGGTAATCGTAGTGTTCCACAATCAGGACCGACTGTACGACCACAAGAA
NM_004874.3       CCTTATGGAGATGGTAATCGTAGTGTTCCACAATCAGGACCGACTGTACGACCACAAGAA
                  ************************************************************

HsCD00515713      GATGCGTGGGCTTCTCCTGGTGCTTATGGAATGGGTGGCCGTTATCCCTGGCCTTCATCA
NM_004874.3       GATGCGTGGGCTTCTCCTGGTGCTTATGGAATGGGTGGCCGTTATCCCTGGCCTTCATCA
                  ************************************************************

HsCD00515713      GCGCCCTCAGCACCACCCGGCAATCTCTACATGACTGAAAGTACTTCACCATGGCCTAGC
NM_004874.3       GCGCCCTCAGCACCACCCGGCAATCTCTACATGACTGAAAGTACTTCACCATGGCCTAGC
                  ************************************************************

HsCD00515713      AGTGGCTCTCCCCAGTCACCCCCTTCACCCCCAGTCCAGCAGCCCAAGGATTCTTCATAC
NM_004874.3       AGTGGCTCTCCCCAGTCACCCCCTTCACCCCCAGTCCAGCAGCCCAAGGATTCTTCATAC
                  ************************************************************

HsCD00515713      CCCTATAGCCAATCAGATCAAAGCATGAACCGGCACAACTTTCCTTGCAGTGTCCATCAG
NM_004874.3       CCCTATAGCCAATCAGATCAAAGCATGAACCGGCACAACTTTCCTTGCAGTGTCCATCAG
                  ************************************************************

HsCD00515713      TACGAATCCTCGGGGACAGTGAACAATGATGATTCAGATCTTTTGGATTCCCAAGTCCAG
NM_004874.3       TACGAATCCTCGGGGACAGTGAACAATGATGATTCAGATCTTTTGGATTCCCAAGTCCAG
                  ************************************************************

HsCD00515713      TATAGTGCTGAGCCTCAGCTGTATGGTAATGCCACCAGTGACCATCCCAACAATCAAGAT
NM_004874.3       TATAGTGCTGAGCCTCAGCTGTATGGTAATGCCACCAGTGACCATCCCAACAATCAAGAT
                  ************************************************************

HsCD00515713      CAAAGTAGCAGTCTTCCTGAAGAATGTGTACCTTCAGATGAAAGTACTCCTCCGAGTATT
NM_004874.3       CAAAGTAGCAGTCTTCCTGAAGAATGTGTACCTTCAGATGAAAGTACTCCTCCGAGTATT
                  ************************************************************

HsCD00515713      AAAAAAATCATACATGTGCTGGAGAAGGTCCAGTATCTTGAACAAGAAGTAGAAGAATTT
NM_004874.3       AAAAAAATCATACATGTGCTGGAGAAGGTCCAGTATCTTGAACAAGAAGTAGAAGAATTT
                  ************************************************************

HsCD00515713      GTAGGAAAAAAGACAGACAAAGCATACTGGCTTCTGGAAGAAATGCTAACCAAGGAACTT
NM_004874.3       GTAGGAAAAAAGACAGACAAAGCATACTGGCTTCTGGAAGAAATGCTAACCAAGGAACTT
                  ************************************************************

HsCD00515713      TTGGAACTGGATTCAGTTGAAACTGGGGGCCAGGACTCTGTACGGCAGGCCAGAAAAGAG
NM_004874.3       TTGGAACTGGATTCAGTTGAAACTGGGGGCCAGGACTCTGTACGGCAGGCCAGAAAAGAG
                  ************************************************************

HsCD00515713      GCTGTTTGTAAGATTCAGGCCATACTGGAAAAATTAGAAAAAAAAGGATTA---
NM_004874.3       GCTGTTTGTAAGATTCAGGCCATACTGGAAAAATTAGAAAAAAAAGGATTATGA
                  ***************************************************