DNASU Plasmid | NAP1L2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NAP1L2

Gene Name: nucleosome assembly protein 1-like 2

Original Clone ID:

ccsbBroadEn_06615

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC026325.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515719

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1381nts

Open reading frame: 1 to 1381

Reference Sequence Alignment

ATGGCCAAGTCAGAGAACCGCAAGGAGCTGTCAGAATCCAGTCAAGAAGAGGCTGGTAAT
CAGATAATGGTGGAAGGGCTCGGGGAACATCTGGAGCGCGGTGAAGATGCCGCTGCTGGG
CTTGGAGACGATGGGAAGTGCGGTGAAGAAGCTGCCGCTGGGCTTGGGGAAGAAGGGGAA
AACGGTGAAGATACTGCTGCTGGGTCCGGGGAAGATGGGAAAAAAGGTGGCGATACTGAT
GAGGACTCAGAGGCAGACCGTCCAAAAGGACTTATCGGTTATGTTTTAGATACAGACTTT
GTTGAAAGTCTACCTGTGAAAGTTAAGTACCGTGTGTTAGCCCTTAAAAAGCTTCAAACT
AGAGCGGCCAATTTAGAATCCAAATTCCTGAGGGAATTTCATGACATTGAAAGAAAGTTT
GCTGAAATGTACCAACCCTTACTGGAAAAAAGACGTCAGATCATCAATGCAATCTATGAA
CCTACAGAAGAGGAATGTGAATATAAATCAGACTCTGAGGACTGTGATGATGAGGAAATG
TGTCATGAAGAGATGTATGGTAATGAGGAGGGTATGGTACATGAATATGTGGATGAGGAC
GATGGTTATGAGGACTATTATTATGATTATGCTGTGGAAGAGGAGGAGGAGGAGGAGGAG
GAGGACGACATTGAGGCTACTGGAGAAGAGAATAAAGAAGAGGAGGATCCTAAGGGAATT
CCTGATTTTTGGCTAACTGTTTTAAAAAACGTTGATACACTCACTCCTTTGATTAAGAAA
TATGATGAGCCTATTCTGAAGCTCCTGACAGATATTAAAGTTAAGCTTTCAGATCCTGGC
GAGCCCCTCAGTTTCACACTAGAATTTCACTTCAAACCCAATGAATATTTCAAAAATGAG
TTGTTGACAAAGACCTATGTGCTGAAGTCAAAGCTAGCATATTATGATCCCCATCCCTAT
AGGGGAACTGCGATTGAGTATTCCACAGGCTGTGAGATAGATTGGAATGAAGGAAAGAAT
GTCACTTTGAAAACCATCAAGAAGAAACAGAAACATCGGATCTGGGGAACAATCCGAACT
GTAACTGAAGATTTTCCCAAGGATTCATTTTTCAATTTTTTCTCTCCTCATGGAATCACC
TCAAATGGAAGGGATGGAAATGATGATTTTTTACTTGGTCACAATTTACGTACTTACATA
ATTCCAAGATCAGTATTATTTTTCTCAGGTGATGCACTGGAATCTCAGCAGGAGGGGGTA
GTTAGAGAAGTTAATGATGCAATTTATGACAAAATTATTTATGATAATTGGATGGCTGCA
ATTGAGGAAGTTAAAGCTTGTTGCAAAAACCTTGAGGCATTAGTAGAAGACATTGATCGT

Protein sequence predicted based on nucleotide sequence

MAKSENRKELSESSQEEAGNQIMVEGLGEHLERGEDAAAGLGDDGKCGEEAAAGLGEEGE
NGEDTAAGSGEDGKKGGDTDEDSEADRPKGLIGYVLDTDFVESLPVKVKYRVLALKKLQT
RAANLESKFLREFHDIERKFAEMYQPLLEKRRQIINAIYEPTEEECEYKSDSEDCDDEEM
CHEEMYGNEEGMVHEYVDEDDGYEDYYYDYAVEEEEEEEEEDDIEATGEENKEEEDPKGI
PDFWLTVLKNVDTLTPLIKKYDEPILKLLTDIKVKLSDPGEPLSFTLEFHFKPNEYFKNE
LLTKTYVLKSKLAYYDPHPYRGTAIEYSTGCEIDWNEGKNVTLKTIKKKQKHRIWGTIRT
VTEDFPKDSFFNFFSPHGITSNGRDGNDDFLLGHNLRTYIIPRSVLFFSGDALESQQEGV
VREVNDAIYDKIIYDNWMAAIEEVKACCKNLEALVEDIDR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NAP1L2

Symbol Nomenclature :

NAP1L2

SYNONYM :

BPX

Designation :

brain specific gene BPX|brain-specific protein, X-linked

Full Nomenclature :

nucleosome assembly protein 1-like 2

GENEID :

4674

HGNC :

7638

MIM :

300026

Vega :

OTTHUMG00000021827

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515719      ATGGCCAAGTCAGAGAACCGCAAGGAGCTGTCAGAATCCAGTCAAGAAGAGGCTGGTAAT
NM_021963.3       ATGGCCGAGTCAGAGAACCGCAAGGAGCTGTCAGAATCCAGTCAAGAAGAGGCTGGTAAT
                  ******.*****************************************************

HsCD00515719      CAGATAATGGTGGAAGGGCTCGGGGAACATCTGGAGCGCGGTGAAGATGCCGCTGCTGGG
NM_021963.3       CAGATAATGGTGGAAGGGCTCGGGGAACATCTGGAGCGCGGTGAAGATGCCGCTGCTGGG
                  ************************************************************

HsCD00515719      CTTGGAGACGATGGGAAGTGCGGTGAAGAAGCTGCCGCTGGGCTTGGGGAAGAAGGGGAA
NM_021963.3       CTTGGAGACGATGGGAAGTGCGGTGAAGAAGCTGCCGCTGGGCTTGGGGAAGAAGGGGAA
                  ************************************************************

HsCD00515719      AACGGTGAAGATACTGCTGCTGGGTCCGGGGAAGATGGGAAAAAAGGTGGCGATACTGAT
NM_021963.3       AACGGTGAAGATACTGCTGCTGGGTCCGGGGAAGATGGGAAAAAAGGTGGCGATACTGAT
                  ************************************************************

HsCD00515719      GAGGACTCAGAGGCAGACCGTCCAAAAGGACTTATCGGTTATGTTTTAGATACAGACTTT
NM_021963.3       GAGGACTCAGAGGCAGACCGTCCAAAAGGACTTATCGGTTATGTTTTAGATACAGACTTT
                  ************************************************************

HsCD00515719      GTTGAAAGTCTACCTGTGAAAGTTAAGTACCGTGTGTTAGCCCTTAAAAAGCTTCAAACT
NM_021963.3       GTTGAAAGTCTACCTGTGAAAGTTAAGTACCGTGTGTTAGCCCTTAAAAAGCTTCAAACT
                  ************************************************************

HsCD00515719      AGAGCGGCCAATTTAGAATCCAAATTCCTGAGGGAATTTCATGACATTGAAAGAAAGTTT
NM_021963.3       AGAGCGGCCAATTTAGAATCCAAATTCCTGAGGGAATTTCATGACATTGAAAGAAAGTTT
                  ************************************************************

HsCD00515719      GCTGAAATGTACCAACCCTTACTGGAAAAAAGACGTCAGATCATCAATGCAATCTATGAA
NM_021963.3       GCTGAAATGTACCAACCCTTACTGGAAAAAAGACGTCAGATCATCAATGCAATCTATGAA
                  ************************************************************

HsCD00515719      CCTACAGAAGAGGAATGTGAATATAAATCAGACTCTGAGGACTGTGATGATGAGGAAATG
NM_021963.3       CCTACAGAAGAGGAATGTGAATATAAATCAGACTCTGAGGACTGTGATGATGAGGAAATG
                  ************************************************************

HsCD00515719      TGTCATGAAGAGATGTATGGTAATGAGGAGGGTATGGTACATGAATATGTGGATGAGGAC
NM_021963.3       TGTCATGAAGAGATGTATGGTAATGAGGAGGGTATGGTACATGAATATGTGGATGAGGAC
                  ************************************************************

HsCD00515719      GATGGTTATGAGGACTATTATTATGATTATGCTGTGGAAGAGGAGGAGGAGGAGGAGGAG
NM_021963.3       GATGGTTATGAGGACTATTATTATGATTATGCTGTGGAAGAGGAGGAGGAGGAGGAGGAG
                  ************************************************************

HsCD00515719      GAGGACGACATTGAGGCTACTGGAGAAGAGAATAAAGAAGAGGAGGATCCTAAGGGAATT
NM_021963.3       GAGGACGACATTGAGGCTACTGGAGAAGAGAATAAAGAAGAGGAGGATCCTAAGGGAATT
                  ************************************************************

HsCD00515719      CCTGATTTTTGGCTAACTGTTTTAAAAAACGTTGATACACTCACTCCTTTGATTAAGAAA
NM_021963.3       CCTGATTTTTGGCTAACTGTTTTAAAAAACGTTGATACACTCACTCCTTTGATTAAGAAA
                  ************************************************************

HsCD00515719      TATGATGAGCCTATTCTGAAGCTCCTGACAGATATTAAAGTTAAGCTTTCAGATCCTGGC
NM_021963.3       TATGATGAGCCTATTCTGAAGCTCCTGACAGATATTAAAGTTAAGCTTTCAGATCCTGGC
                  ************************************************************

HsCD00515719      GAGCCCCTCAGTTTCACACTAGAATTTCACTTCAAACCCAATGAATATTTCAAAAATGAG
NM_021963.3       GAGCCCCTCAGTTTCACACTAGAATTTCACTTCAAACCCAATGAATATTTCAAAAATGAG
                  ************************************************************

HsCD00515719      TTGTTGACAAAGACCTATGTGCTGAAGTCAAAGCTAGCATATTATGATCCCCATCCCTAT
NM_021963.3       TTGTTGACAAAGACCTATGTGCTGAAGTCAAAGCTAGCATATTATGATCCCCATCCCTAT
                  ************************************************************

HsCD00515719      AGGGGAACTGCGATTGAGTATTCCACAGGCTGTGAGATAGATTGGAATGAAGGAAAGAAT
NM_021963.3       AGGGGAACTGCGATTGAGTATTCCACAGGCTGTGAGATAGATTGGAATGAAGGAAAGAAT
                  ************************************************************

HsCD00515719      GTCACTTTGAAAACCATCAAGAAGAAACAGAAACATCGGATCTGGGGAACAATCCGAACT
NM_021963.3       GTCACTTTGAAAACCATCAAGAAGAAACAGAAACATCGGATCTGGGGAACAATCCGAACT
                  ************************************************************

HsCD00515719      GTAACTGAAGATTTTCCCAAGGATTCATTTTTCAATTTTTTCTCTCCTCATGGAATCACC
NM_021963.3       GTAACTGAAGATTTTCCCAAGGATTCATTTTTCAATTTTTTCTCTCCTCATGGAATCACC
                  ************************************************************

HsCD00515719      TCAAATGGAAGGGATGGAAATGATGATTTTTTACTTGGTCACAATTTACGTACTTACATA
NM_021963.3       TCAAATGGAAGGGATGGAAATGATGATTTTTTACTTGGTCACAATTTACGTACTTACATA
                  ************************************************************

HsCD00515719      ATTCCAAGATCAGTATTATTTTTCTCAGGTGATGCACTGGAATCTCAGCAGGAGGGGGTA
NM_021963.3       ATTCCAAGATCAGTATTATTTTTCTCAGGTGATGCACTGGAATCTCAGCAGGAGGGGGTA
                  ************************************************************

HsCD00515719      GTTAGAGAAGTTAATGATGCAATTTATGACAAAATTATTTATGATAATTGGATGGCTGCA
NM_021963.3       GTTAGAGAAGTTAATGATGCAATTTATGACAAAATTATTTATGATAATTGGATGGCTGCA
                  ************************************************************

HsCD00515719      ATTGAGGAAGTTAAAGCTTGTTGCAAAAACCTTGAGGCATTAGTAGAAGACATTGATCGT
NM_021963.3       ATTGAGGAAGTTAAAGCTTGTTGCAAAAACCTTGAGGCATTAGTAGAAGACATTGATCGT
                  ************************************************************

HsCD00515719      ---
NM_021963.3       TAG