DNASU Plasmid | TIGD3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TIGD3

Gene Name: tigger transposable element derived 3

Original Clone ID:

ccsbBroadEn_05243

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC074862.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515740

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1414nts

Open reading frame: 1 to 1414

Reference Sequence Alignment

ATGGAGCTGAGCAGCAAGAAGAAGCTTCACGCCCTGTCCCTGGCCGAGAAGATCCAGGTG
CTGGAACTCCTGGATGAGTCCAAGATGTCCCAGTCGGAGGTGGCCCGGCGCTTCCAGGTT
TCCCAGCCCCAGATCTCGCGCATCTGCAAGAATAAGGAGAAGCTGCTGGCGGACTGGTGC
AGCGGCACAGCCAACCGAGAGCGCAAGCGCAAGCGGGAGTCCAAGTACAGCGGGATCGAC
GAGGCTCTGCTCTGCTGGTACCACATTGCCCGGGCCAAGGCCTGGGACGTGACGGGGCCC
ATGCTGCTCCACAAAGCCAAGGAGCTGGCCGATATCATGGGCCAGGACTTCGTGCCCAGC
ATCGGCTGGCTGGTCCGCTGGAAACGCCGAAACAACGTCGGCTTTGGGGCCCGCCATGTT
CTTGCGCCTTCATTCCCCCCTGAGCCACCTCCCCCGGGGCTCACATCCCAGGCTCAGCTG
CCTCTTTCCCTAAAAGACTTCTCTCCAGAGGACGTGTTTGGCTGTGCTGAATTGCCCTTG
CTGTATCGGGCAGTGCCCGGCAGCTTTGGTGCATGTGATCAAGTACAGGTGCTGCTGTGT
GCCAACAGCAGGGGCACCGAGAAGCGGCGGGTACTGCTGGGTGGGCTCCAGGCTGCCCCG
AGATGCTTCTTTGGGATCCGCAGTGAGGCTCTGCCTGCCTCCTACCACCCGGACCTGGGC
ATCCCCTGGTTAGAGTGGTTGGCACAGTTTGACCGGGACATGGGACAGCAGGGCCGACAG
GTGGCTTTGCTGCTGGCTGCCCGAGTGGTGGAGGAGCTGGCAGGCCTGCCTGGGCTCTAC
CACGTGAAGCTCTTGCCTCTGGCCGCCTCTAGCACCACGCCTCCCCTGCCCAGCTCAGTG
GTCCGGGCCTTTAAGGCCCATTACCGACACCGGCTGTTGGGCAAACTGGCTGCCATCCAA
AGCGAGAGGGATGGCACCTCGCTGGCCGAGGCCGGGGCAGGCATCACCGTGCTGGACGCC
CTGCACGTGGCGTCTGCCGCCTGGGCCAAGGTGCCTCCTCAGCTCATTTTCAGCAGCTTC
ATTCAAGAAGGGCTGGCTCCCGGCAAAACGCCCCCGTCCTCGCACAAAACCTCTGAGATG
CCACCAGTCCCCGGCGGGCTGAGCCTGGAGGAGTTTTCCCGCTTTGTGGACCTGGAGGGT
GAGGAGCCAAGGTCTGGAGTATGTAAGGAGGAGATAGGCACTGAAGACGAGAAGGGGGAC
AGAGAGGGTGCCTTTGAGCCCCTGCCCACCAAAGCTGATGCCCTCCGGGCCCTGGGCACC
TTGAGGAGGTGGTTTGAATGCAACAGCACTTCTCCTGAGCTATTCGAAAAATTCTACGAC
TGTGAGGAGGAGGTGGAGCGGCTTTGCTGCCTA

Protein sequence predicted based on nucleotide sequence

MELSSKKKLHALSLAEKIQVLELLDESKMSQSEVARRFQVSQPQISRICKNKEKLLADWC
SGTANRERKRKRESKYSGIDEALLCWYHIARAKAWDVTGPMLLHKAKELADIMGQDFVPS
IGWLVRWKRRNNVGFGARHVLAPSFPPEPPPPGLTSQAQLPLSLKDFSPEDVFGCAELPL
LYRAVPGSFGACDQVQVLLCANSRGTEKRRVLLGGLQAAPRCFFGIRSEALPASYHPDLG
IPWLEWLAQFDRDMGQQGRQVALLLAARVVEELAGLPGLYHVKLLPLAASSTTPPLPSSV
VRAFKAHYRHRLLGKLAAIQSERDGTSLAEAGAGITVLDALHVASAAWAKVPPQLIFSSF
IQEGLAPGKTPPSSHKTSEMPPVPGGLSLEEFSRFVDLEGEEPRSGVCKEEIGTEDEKGD
REGAFEPLPTKADALRALGTLRRWFECNSTSPELFEKFYDCEEEVERLCCL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TIGD3

Symbol Nomenclature :

TIGD3

Designation :

tigger transposable element-derived protein 3

Full Nomenclature :

tigger transposable element derived 3

GENEID :

220359

HGNC :

18334

Vega :

OTTHUMG00000165986

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515740      ATGGAGCTGAGCAGCAAGAAGAAGCTTCACGCCCTGTCCCTGGCCGAGAAGATCCAGGTG
NM_145719.2       ATGGAGCTGAGCAGCAAGAAGAAGCTTCACGCCCTGTCCCTGGCCGAGAAGATCCAGGTG
                  ************************************************************

HsCD00515740      CTGGAACTCCTGGATGAGTCCAAGATGTCCCAGTCGGAGGTGGCCCGGCGCTTCCAGGTT
NM_145719.2       CTGGAACTCCTGGATGAGTCCAAGATGTCCCAGTCGGAGGTGGCCCGGCGCTTCCAGGTT
                  ************************************************************

HsCD00515740      TCCCAGCCCCAGATCTCGCGCATCTGCAAGAATAAGGAGAAGCTGCTGGCGGACTGGTGC
NM_145719.2       TCCCAGCCCCAGATCTCGCGCATCTGCAAGAATAAGGAGAAGCTGCTGGCGGACTGGTGC
                  ************************************************************

HsCD00515740      AGCGGCACAGCCAACCGAGAGCGCAAGCGCAAGCGGGAGTCCAAGTACAGCGGGATCGAC
NM_145719.2       AGCGGCACAGCCAACCGAGAGCGCAAGCGCAAGCGGGAGTCCAAGTACAGCGGGATCGAC
                  ************************************************************

HsCD00515740      GAGGCTCTGCTCTGCTGGTACCACATTGCCCGGGCCAAGGCCTGGGACGTGACGGGGCCC
NM_145719.2       GAGGCTCTGCTCTGCTGGTACCACATTGCCCGGGCCAAGGCCTGGGACGTGACGGGGCCC
                  ************************************************************

HsCD00515740      ATGCTGCTCCACAAAGCCAAGGAGCTGGCCGATATCATGGGCCAGGACTTCGTGCCCAGC
NM_145719.2       ATGCTGCTCCACAAAGCCAAGGAGCTGGCCGATATCATGGGCCAGGACTTCGTGCCCAGC
                  ************************************************************

HsCD00515740      ATCGGCTGGCTGGTCCGCTGGAAACGCCGAAACAACGTCGGCTTTGGGGCCCGCCATGTT
NM_145719.2       ATCGGCTGGCTGGTCCGCTGGAAACGCCGAAACAACGTCGGCTTTGGGGCCCGCCATGTT
                  ************************************************************

HsCD00515740      CTTGCGCCTTCATTCCCCCCTGAGCCACCTCCCCCGGGGCTCACATCCCAGGCTCAGCTG
NM_145719.2       CTTGCGCCTTCATTCCCCCCTGAGCCACCTCCCCCGGGGCTCACATCCCAGGCTCAGCTG
                  ************************************************************

HsCD00515740      CCTCTTTCCCTAAAAGACTTCTCTCCAGAGGACGTGTTTGGCTGTGCTGAATTGCCCTTG
NM_145719.2       CCTCTTTCCCTAAAAGACTTCTCTCCAGAGGACGTGTTTGGCTGTGCTGAATTGCCCTTG
                  ************************************************************

HsCD00515740      CTGTATCGGGCAGTGCCCGGCAGCTTTGGTGCATGTGATCAAGTACAGGTGCTGCTGTGT
NM_145719.2       CTGTATCGGGCAGTGCCCGGCAGCTTTGGTGCATGTGATCAAGTACAGGTGCTGCTGTGT
                  ************************************************************

HsCD00515740      GCCAACAGCAGGGGCACCGAGAAGCGGCGGGTACTGCTGGGTGGGCTCCAGGCTGCCCCG
NM_145719.2       GCCAACAGCAGGGGCACCGAGAAGCGGCGGGTACTGCTGGGTGGGCTCCAGGCTGCCCCG
                  ************************************************************

HsCD00515740      AGATGCTTCTTTGGGATCCGCAGTGAGGCTCTGCCTGCCTCCTACCACCCGGACCTGGGC
NM_145719.2       AGATGCTTCTTTGGGATCCGCAGTGAGGCTCTGCCTGCCTCCTACCACCCGGACCTGGGC
                  ************************************************************

HsCD00515740      ATCCCCTGGTTAGAGTGGTTGGCACAGTTTGACCGGGACATGGGACAGCAGGGCCGACAG
NM_145719.2       ATCCCCTGGTTAGAGTGGTTGGCACAGTTTGACCGGGACATGGGACAGCAGGGCCGACAG
                  ************************************************************

HsCD00515740      GTGGCTTTGCTGCTGGCTGCCCGAGTGGTGGAGGAGCTGGCAGGCCTGCCTGGGCTCTAC
NM_145719.2       GTGGCTTTGCTGCTGGCTGCCCGAGTGGTGGAGGAGCTGGCAGGCCTGCCTGGGCTCTAC
                  ************************************************************

HsCD00515740      CACGTGAAGCTCTTGCCTCTGGCCGCCTCTAGCACCACGCCTCCCCTGCCCAGCTCAGTG
NM_145719.2       CACGTGAAGCTCTTGCCTCTGGCCGCCTCTAGCACCACGCCTCCCCTGCCCAGCTCAGTG
                  ************************************************************

HsCD00515740      GTCCGGGCCTTTAAGGCCCATTACCGACACCGGCTGTTGGGCAAACTGGCTGCCATCCAA
NM_145719.2       GTCCGGGCCTTTAAGGCCCATTACCGACACCGGCTGTTGGGCAAACTGGCTGCCATCCAA
                  ************************************************************

HsCD00515740      AGCGAGAGGGATGGCACCTCGCTGGCCGAGGCCGGGGCAGGCATCACCGTGCTGGACGCC
NM_145719.2       AGCGAGAGGGATGGCACCTCGCTGGCCGAGGCCGGGGCAGGCATCACCGTGCTGGACGCC
                  ************************************************************

HsCD00515740      CTGCACGTGGCGTCTGCCGCCTGGGCCAAGGTGCCTCCTCAGCTCATTTTCAGCAGCTTC
NM_145719.2       CTGCACGTGGCGTCTGCCGCCTGGGCCAAGGTGCCTCCTCAGCTCATTTTCAGCAGCTTC
                  ************************************************************

HsCD00515740      ATTCAAGAAGGGCTGGCTCCCGGCAAAACGCCCCCGTCCTCGCACAAAACCTCTGAGATG
NM_145719.2       ATTCAAGAAGGGCTGGCTCCCGGCAAAACGCCCCCGTCCTCGCACAAAACCTCTGAGATG
                  ************************************************************

HsCD00515740      CCACCAGTCCCCGGCGGGCTGAGCCTGGAGGAGTTTTCCCGCTTTGTGGACCTGGAGGGT
NM_145719.2       CCACCAGTCCCCGGCGGGCTGAGCCTGGAGGAGTTTTCCCGCTTTGTGGACCTGGAGGGT
                  ************************************************************

HsCD00515740      GAGGAGCCAAGGTCTGGAGTATGTAAGGAGGAGATAGGCACTGAAGACGAGAAGGGGGAC
NM_145719.2       GAGGAGCCAAGGTCTGGAGTATGTAAGGAGGAGATAGGCACTGAAGACGAGAAGGGGGAC
                  ************************************************************

HsCD00515740      AGAGAGGGTGCCTTTGAGCCCCTGCCCACCAAAGCTGATGCCCTCCGGGCCCTGGGCACC
NM_145719.2       AGAGAGGGTGCCTTTGAGCCCCTGCCCACCAAAGCTGATGCCCTCCGGGCCCTGGGCACC
                  ************************************************************

HsCD00515740      TTGAGGAGGTGGTTTGAATGCAACAGCACTTCTCCTGAGCTATTCGAAAAATTCTACGAC
NM_145719.2       TTGAGGAGGTGGTTTGAATGCAACAGCACTTCTCCTGAGCTATTCGAAAAATTCTACGAC
                  ************************************************************

HsCD00515740      TGTGAGGAGGAGGTGGAGCGGCTTTGCTGCCTA---
NM_145719.2       TGTGAGGAGGAGGTGGAGCGGCTTTGCTGCCTATGA
                  *********************************