ATP6V1H (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ATP6V1H
Gene Name:  ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Original Clone ID:
ccsbBroadEn_03343
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC025275.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00515827
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1450nts
Open reading frame: 1 to 1450

ATGACCAAAATGGATATCCGAGGTGCTGTGGATGCTGCTGTCCCCACCAATATTATTGCT
GCCAAGGCTGCAGAAGTTCGTGCAAACAAAGTCAACTGGCAATCCTATCTTCAGGGACAG
ATGATTTCTGCTGAAGATTGTGAGTTTATTCAGAGGTTTGAAATGAAACGAAGCCCTGAA
GAGAAGCAAGAGATGCTTCAAACTGAAGGCAGCCAGTGTGCTAAAACATTTATAAATCTG
ATGACTCATATCTGCAAAGAACAGACCGTTCAGTATATACTAACTATGGTGGATGATATG
CTGCAGGAAAATCATCAGCGTGTTAGCATTTTCTTTGACTATGCAAGATGTAGCAAGAAC
ACTGCGTGGCCCTACTTTCTGCCAATGTTGAATCGCCAGGATCCCTTCACTGTTCATATG
GCAGCAAGAATTATTGCCAAGTTAGCAGCTTGGGGAAAAGAACTGATGGAAGGCAGTGAC
TTAAATTACTATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTGCGTGGTAGC
GGTGTTGCTGTTGAAACAGGAACAGTCTCTTCAAGTGATAGTTCGCAGTATGTGCAGTGC
GTGGCCGGGTGTTTGCAGCTGATGCTCCGGGTCAATGAGTACCGCTTTGCTTGGGTGGAA
GCAGATGGGGTAAATTGCATAATGGGAGTGTTGAGTAACAAGTGTGGCTTTCAGCTCCAG
TATCAAATGATTTTTTCAATATGGCTCCTGGCATTCAGTCCTCAAATGTGTGAACACCTG
CGGCGCTATAATATCATTCCAGTTCTGTCTGATATCCTTCAGGAGTCTGTCAAAGAGAAA
GTAACAAGAATCATTCTTGCAGCATTTCGTAACTTTTTAGAAAAATCAACTGAAAGAGAA
ACTCGCCAAGAATATGCCCTGGCTATGATTCAGTGCAAAGTTCTGAAACAGTTGGAGAAC
TTGGAACAGCAGAAGTACGATGATGAAGATATCAGCGAAGATATCAAATTTCTTTTGGAA
AAACTTGGAGAGAGTGTCCAGGACCTTAGTTCATTTGATGAATACAGTTCAGAACTTAAA
TCTGGAAGGTTGGAATGGAGTCCTGTGCACAAATCTGAGAAATTTTGGAGAGAGAATGCT
GTGAGGTTAAATGAGAAGAATTATGAACTCTTGAAAATCTTGACAAAACTTTTGGAAGTG
TCAGATGATCCCCAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATGTGCGGCAT
TATCCACGAGGCAAACGGGTCATCGAGCAGCTCGGTGGGAAGCAGCTGGTCATGAACCAC
ATGCATCATGAAGACCAGCAGGTCCGCTATAATGCTCTGCTGGCCGTGCAGAAGCTCATG
GTGCACAACTGGGAATACCTTGGCAAGCAGCTCCAGTCCGAGCAGCCCCAGACCGCTGCC
GCCCGAAGC

Protein sequence predicted based on nucleotide sequence

MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSPE
EKQEMLQTEGSQCAKTFINLMTHICKEQTVQYILTMVDDMLQENHQRVSIFFDYARCSKN
TAWPYFLPMLNRQDPFTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGS
GVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQ
YQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERE
TRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELK
SGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRH
YPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAA
ARS

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable