DNASU Plasmid | WASF3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: WASF3

Gene Name: WAS protein family, member 3

Original Clone ID:

ccsbBroadEn_11555

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC050283.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515853

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1498nts

Open reading frame: 1 to 1498

Reference Sequence Alignment

ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAA
GGGATTACCAGCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAG
CTGAGCAGTCTGAGCAAACATGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAAC
AACTTCTACATCAGAGCAAATTCTCTTCAAGACAGAATTGATCGCCTTGCTGTCAAAGTC
ACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTACAGGATATCAACATGAAAAAAGCT
TTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAACAGCATTCCTAATCCT
GTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACACCATAC
AGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTC
TGGAAAGAAAAAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAG
AGAGAGAAACACAAGCTGAACCCTAACAGAAACCAGCAAGTAAATGTGAGAAAAGTCAGA
ACAAGAAAAGAAGAGTGGGAGAGAAGGAAAATGGGCATTGAGTTTATGAGTGACGCAAAG
AAACTGGAGCAGGCAGGGAGCGCCAAAGAGGACAGAGTGCCCAGCGGGTCACATGCATCG
GACGTTACGGATTACTCTTACCCGGCTACTCCCAACCATTCTCTGCACCCCCAGCCTGTG
ACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGCCAGGCTGCGGAG
CATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAGCAG
CCGCCCCCCCCGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCT
CCAGCAGACTACGGGATGCTCCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCA
CCTCCTCCGCCACCTCCTCCTGTGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTC
CAGATGCCCATGCAGCCCCCGTTCCCTGCATCAGCCAGCTCCACGCACGCAGCTCCTCCT
CACCCACCCTCCACCGGGCTCCTGGTCACAGCCCCGCCACCCCCGGGCCCACCACCTCCC
CCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCCCCAATGCATGGCCCCCCA
GTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCTCGAAGCGAC
CTCCTCGCTGCTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAG
GAGGCCAAGCGGGAGCCAGTGGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCC
GTGGAGTACAGCGACTCTGACGACGACTCAGAGTTCGACGAGAACGACTGGTCCGAC

Protein sequence predicted based on nucleotide sequence

MPLVKRNIEPRHLCRGALPEGITSELECVTNSTLAAIIRQLSSLSKHAEDIFGELFNEAN
NFYIRANSLQDRIDRLAVKVTQLDSTVEEVSLQDINMKKAFKSSTVQDQQVVSKNSIPNP
VADIYNQSDKPPPLNILTPYRDDKKDGLKFYTDPSYFFDLWKEKMLQDTEDKRKEKRRQK
REKHKLNPNRNQQVNVRKVRTRKEEWERRKMGIEFMSDAKKLEQAGSAKEDRVPSGSHAS
DVTDYSYPATPNHSLHPQPVTPSYAAGDVPPHGPASQAAEHEYRPPSASARHMALNRPQQ
PPPPPPPQAPEGSQASAPMAPADYGMLPAQIIEYYNPSGPPPPPPPPVIPSAQTAFVSPL
QMPMQPPFPASASSTHAAPPHPPSTGLLVTAPPPPGPPPPPPGPPGPGSSLSSSPMHGPP
VAEAKRQEPAQPPISDARSDLLAAIRMGIQLKKVQEQREQEAKREPVGNDVATILSRRIA
VEYSDSDDDSEFDENDWSD

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

WASF3

Symbol Nomenclature :

WASF3

SYNONYM :

Brush-1; SCAR3; WAVE3

Designation :

WASP family Verprolin-homologous protein 3|WASP family protein member 3|protein WAVE-3|verprolin homology domain-containing protein 3|wiskott-Aldrich syndrome protein family member 3

Full Nomenclature :

WAS protein family, member 3

GENEID :

10810

Locus Tag :

RP11-496O3.2

HGNC :

12734

MIM :

605068

Vega :

OTTHUMG00000016621

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515853        ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAA
XM_005266239.1      ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAA
                    ************************************************************

HsCD00515853        GGGATTACCAGCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAG
XM_005266239.1      GGGATTACCAGCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAG
                    ************************************************************

HsCD00515853        CTGAGCAGTCTGAGCAAACATGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAAC
XM_005266239.1      CTGAGCAGTCTGAGCAAACATGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAAC
                    ************************************************************

HsCD00515853        AACTTCTACATCAGAGCAAATTCTCTTCAAGACAGAATTGATCGCCTTGCTGTCAAAGTC
XM_005266239.1      AACTTCTACATCAGAGCAAATTCTCTTCAAGACAGAATTGATCGCCTTGCTGTCAAAGTC
                    ************************************************************

HsCD00515853        ACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTACAGGATATCAACATGAAAAAAGCT
XM_005266239.1      ACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTACAGGATATCAACATGAAAAAAGCT
                    ************************************************************

HsCD00515853        TTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAACAGCATTCCTAATCCT
XM_005266239.1      TTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAACAGCATTCCTAATCCT
                    ************************************************************

HsCD00515853        GTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACACCATAC
XM_005266239.1      GTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACACCATAC
                    ************************************************************

HsCD00515853        AGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTC
XM_005266239.1      AGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTC
                    ************************************************************

HsCD00515853        TGGAAAGAAAAAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAG
XM_005266239.1      TGGAAAGAAAAAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAG
                    ************************************************************

HsCD00515853        AGAGAGAAACACAAGCTGAACCCTAACAGAAACCAGCAAGTAAATGTGAGAAAAGTCAGA
XM_005266239.1      AGAGAGAAACACAAGCTGAACCCTAACAGAAACCAGCAAGTAAATGTGAGAAAAGTCAGA
                    ************************************************************

HsCD00515853        ACAAGAAAAGAAGAGTGGGAGAGAAGGAAAATGGGCATTGAGTTTATGAGTGACGCAAAG
XM_005266239.1      ACAAGAAAAGAAGAGTGGGAGAGAAGGAAAATGGGCATTGAGTTTATGAGTGACGCAAAG
                    ************************************************************

HsCD00515853        AAACTGGAGCAGGCAGGGAGCGCCAAAGAGGACAGAGTGCCCAGCGGGTCACATGCATCG
XM_005266239.1      AAACTGGAGCAGGCAGGGAGCGCCAAAGAGGACAGAGTGCCCAGCGGGTCACATGCATCG
                    ************************************************************

HsCD00515853        GACGTTACGGATTACTCTTACCCGGCTACTCCCAACCATTCTCTGCACCCCCAGCCTGTG
XM_005266239.1      GACGTTACGGATTACTCTTACCCGGCTACTCCCAACCATTCTCTGCACCCCCAGCCTGTG
                    ************************************************************

HsCD00515853        ACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGCCAGGCTGCGGAG
XM_005266239.1      ACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGCCAGGCTGCGGAG
                    ************************************************************

HsCD00515853        CATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAGCAG
XM_005266239.1      CATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAGCAG
                    ************************************************************

HsCD00515853        CCGCCCCCCCCGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCT
XM_005266239.1      CCGCCCCCCCCGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCT
                    ************************************************************

HsCD00515853        CCAGCAGACTACGGGATGCTCCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCA
XM_005266239.1      CCAGCAGACTACGGGATGCTCCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCA
                    ************************************************************

HsCD00515853        CCTCCTCCGCCACCTCCTCCTGTGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTC
XM_005266239.1      CCTCCTCCGCCACCTCCTCCTGTGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTC
                    ************************************************************

HsCD00515853        CAGATGCCCATGCAGCCCCCGTTCCCTGCATCAGCCAGCTCCACGCACGCAGCTCCTCCT
XM_005266239.1      CAGATGCCCATGCAGCCCCCGTTCCCTGCATCAGCCAGCTCCACGCACGCAGCTCCTCCT
                    ************************************************************

HsCD00515853        CACCCACCCTCCACCGGGCTCCTGGTCACAGCCCCGCCACCCCCGGGCCCACCACCTCCC
XM_005266239.1      CACCCACCCTCCACCGGGCTCCTGGTCACAGCCCCGCCACCCCCGGGCCCACCACCTCCC
                    ************************************************************

HsCD00515853        CCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCCCCAATGCATGGCCCCCCA
XM_005266239.1      CCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCCCCAATGCATGGCCCCCCA
                    ************************************************************

HsCD00515853        GTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCTCGAAGCGAC
XM_005266239.1      GTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCTCGAAGCGAC
                    ************************************************************

HsCD00515853        CTCCTCGCTGCTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAG
XM_005266239.1      CTCCTCGCTGCTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAG
                    ************************************************************

HsCD00515853        GAGGCCAAGCGGGAGCCAGTGGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCC
XM_005266239.1      GAGGCCAAGCGGGAGCCAGTGGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCC
                    ************************************************************

HsCD00515853        GTGGAGTACAGCGACTCTGACGACGACTCAGAGTTCGACGAGAACGACTGGTCCGAC---
XM_005266239.1      GTGGAGTACAGCGACTCTGACGACGACTCAGAGTTCGACGAGAACGACTGGTCCGACTGA
                    *********************************************************