DNASU Plasmid | KDELC2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KDELC2

Gene Name: KDEL (Lys-Asp-Glu-Leu) containing 2

Original Clone ID:

ccsbBroadEn_09607

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC036526.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515866

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1522nts

Open reading frame: 1 to 1522

Reference Sequence Alignment

ATGCGCCGCCTCCCGCGGGCCCTGCTGCTGCAGCTGCGCCTCGCCCTGCTGGTGGCCGCG
GGGGCCCCGGAGGTGCTGGTCAGCGCGCCGCGGAGCCTGGTGTGGGGGCCCGGGCTGCAG
GCGGCCGTCGTCCTGCCGGTCCGCTATTTCTACCTGCAGGCGGTCAACTCGGAGGGCCAG
AACCTCACTCGCTCTCCCGCAGGTGAAACACCATTCAAAGTCGTAGTCAAATCTCTTTCA
CCTAAAGAGTTGGTCCGGATACATGTCCCTAAACCTTTGGACAGGAATGATGGAACATTT
TTGATGAGATATAGGATGTATGAAACTGTCGATGAAGGCCTGAAGATAGAGGTCCTTTAT
GGTGATGAACATGTGGCTCAGTCTCCCTATATTTTGAAAGGACCAGTGTACCATGAGTAC
TGTGAGTGTCCGGAAGATCCTCAGGCCTGGCAGAAGACTCTTTCTTGTCCAACCAAGGAA
CCACAGATTGCAAAAGATTTTGCTTCCTTTCCCAGCATCAATCTCCAGCAAATGCTAAAA
GAAGTCCCCAAAAGGTTTGGGGATGAGAGAGGTGCCATTGTTCATTACACGATTCTCAAT
AACCATGTTTACCGGAGATCTTTAGGGAAATACACAGACTTCAAGATGTTCTCTGATGAG
ATTTTGTTATCATTGACAAGAAAGGTCCTTCTCCCAGATTTAGAATTTTATGTTAATCTT
GGAGATTGGCCCTTGGAGCATCGAAAAGTCAATGGAACCCCTAGCCCCATACCTATCATT
TCATGGTGTGGCTCTCTGGATTCAAGAGATGTTGTCCTTCCAACGTATGACATCACCCAC
TCCATGCTTGAAGCCATGCGGGGTGTTACAAATGATCTCCTCTCTATTCAGGGAAATACA
GGGCCTTCCTGGATCAATAAAACAGAGAGAGCTTTCTTCAGAGGTAGAGACAGCCTAGAG
GAGAGGCTCCAGTTGGTACAGCTGTCCAAAGAAAATCCTCAGCTACTAGATGCAGGAATT
ACAGGATATTTCTTTTTCCAAGAGAAAGAAAAGGAGCTTGGAAAAGCCAAGTTGATGGGT
TTCTTTGATTTCTTTAAGTACAAGTATCAAGTAAATGTGGATGGGACCGTGGCTGCTTAC
AGATATCCATATCTCATGCTGGGCGACAGTCTGGTTTTAAAGCAGGACTCGCCATATTAT
GAACATTTCTACATGGCACTAGAACCTTGGAAGCATTATGTTCCAATTAAAAGAAATCTG
AGTGATTTATTAGAGAAAGTTAAATGGGCTAAGGAAAATGATGAAGAAGCCAAGAAGATT
GCAAAAGAAGGACAGTTGATGGCTAGGGACCTACTACAGCCACACAGGCTTTACTGCTAC
TATTACCAAGTACTGCAGAAATATGCCGAGCGCCAGTCCAGCAAACCCGAAGTACGTGAT
GGAATGGAACTTGTTCCTCAGCCAGAAGATAGCACAGCCATCTGCCAGTGCCACAGGAAA
AAGCCTTCAAGAGAAGAACTT

Protein sequence predicted based on nucleotide sequence

MRRLPRALLLQLRLALLVAAGAPEVLVSAPRSLVWGPGLQAAVVLPVRYFYLQAVNSEGQ
NLTRSPAGETPFKVVVKSLSPKELVRIHVPKPLDRNDGTFLMRYRMYETVDEGLKIEVLY
GDEHVAQSPYILKGPVYHEYCECPEDPQAWQKTLSCPTKEPQIAKDFASFPSINLQQMLK
EVPKRFGDERGAIVHYTILNNHVYRRSLGKYTDFKMFSDEILLSLTRKVLLPDLEFYVNL
GDWPLEHRKVNGTPSPIPIISWCGSLDSRDVVLPTYDITHSMLEAMRGVTNDLLSIQGNT
GPSWINKTERAFFRGRDSLEERLQLVQLSKENPQLLDAGITGYFFFQEKEKELGKAKLMG
FFDFFKYKYQVNVDGTVAAYRYPYLMLGDSLVLKQDSPYYEHFYMALEPWKHYVPIKRNL
SDLLEKVKWAKENDEEAKKIAKEGQLMARDLLQPHRLYCYYYQVLQKYAERQSSKPEVRD
GMELVPQPEDSTAICQCHRKKPSREEL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KDELC2

Symbol Nomenclature :

KDELC2

Designation :

KDEL motif-containing protein 2

Full Nomenclature :

KDEL (Lys-Asp-Glu-Leu) containing 2

GENEID :

143888

Locus Tag :

UNQ1904/PRO4350

HGNC :

28496

Vega :

OTTHUMG00000166535

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515866      ATGCGCCGCCTCCCGCGGGCCCTGCTGCTGCAGCTGCGCCTCGCCCTGCTGGTGGCCGCG
NM_153705.4       ATGCGCCGCCTCCCGCGGGCCCTGCTGCTGCAGCTGCGCCTCGCCCTGCTGGTGGCCGCG
                  ************************************************************

HsCD00515866      GGGGCCCCGGAGGTGCTGGTCAGCGCGCCGCGGAGCCTGGTGTGGGGGCCCGGGCTGCAG
NM_153705.4       GGGGCCCCGGAGGTGCTGGTCAGCGCGCCGCGGAGCCTGGTGTGGGGGCCCGGGCTGCAG
                  ************************************************************

HsCD00515866      GCGGCCGTCGTCCTGCCGGTCCGCTATTTCTACCTGCAGGCGGTCAACTCGGAGGGCCAG
NM_153705.4       GCGGCCGTCGTCCTGCCGGTCCGCTATTTCTACCTGCAGGCGGTCAACTCGGAGGGCCAG
                  ************************************************************

HsCD00515866      AACCTCACTCGCTCTCCCGCAGGTGAAACACCATTCAAAGTCGTAGTCAAATCTCTTTCA
NM_153705.4       AACCTCACTCGCTCTCCCGCAGGTGAAACACCATTCAAAGTCGTAGTCAAATCTCTTTCA
                  ************************************************************

HsCD00515866      CCTAAAGAGTTGGTCCGGATACATGTCCCTAAACCTTTGGACAGGAATGATGGAACATTT
NM_153705.4       CCTAAAGAGTTGGTCCGGATACATGTCCCTAAACCTTTGGACAGGAATGATGGAACATTT
                  ************************************************************

HsCD00515866      TTGATGAGATATAGGATGTATGAAACTGTCGATGAAGGCCTGAAGATAGAGGTCCTTTAT
NM_153705.4       TTGATGAGATATAGGATGTATGAAACTGTCGATGAAGGCCTGAAGATAGAGGTCCTTTAT
                  ************************************************************

HsCD00515866      GGTGATGAACATGTGGCTCAGTCTCCCTATATTTTGAAAGGACCAGTGTACCATGAGTAC
NM_153705.4       GGTGATGAACATGTGGCTCAGTCTCCCTATATTTTGAAAGGACCAGTGTACCATGAGTAC
                  ************************************************************

HsCD00515866      TGTGAGTGTCCGGAAGATCCTCAGGCCTGGCAGAAGACTCTTTCTTGTCCAACCAAGGAA
NM_153705.4       TGTGAGTGTCCGGAAGATCCTCAGGCCTGGCAGAAGACTCTTTCTTGTCCAACCAAGGAA
                  ************************************************************

HsCD00515866      CCACAGATTGCAAAAGATTTTGCTTCCTTTCCCAGCATCAATCTCCAGCAAATGCTAAAA
NM_153705.4       CCACAGATTGCAAAAGATTTTGCTTCCTTTCCCAGCATCAATCTCCAGCAAATGCTAAAA
                  ************************************************************

HsCD00515866      GAAGTCCCCAAAAGGTTTGGGGATGAGAGAGGTGCCATTGTTCATTACACGATTCTCAAT
NM_153705.4       GAAGTCCCCAAAAGGTTTGGGGATGAGAGAGGTGCCATTGTTCATTACACGATTCTCAAT
                  ************************************************************

HsCD00515866      AACCATGTTTACCGGAGATCTTTAGGGAAATACACAGACTTCAAGATGTTCTCTGATGAG
NM_153705.4       AACCATGTTTACCGGAGATCTTTAGGGAAATACACAGACTTCAAGATGTTCTCTGATGAG
                  ************************************************************

HsCD00515866      ATTTTGTTATCATTGACAAGAAAGGTCCTTCTCCCAGATTTAGAATTTTATGTTAATCTT
NM_153705.4       ATTTTGTTATCATTGACAAGAAAGGTCCTTCTCCCAGATTTAGAATTTTATGTTAATCTT
                  ************************************************************

HsCD00515866      GGAGATTGGCCCTTGGAGCATCGAAAAGTCAATGGAACCCCTAGCCCCATACCTATCATT
NM_153705.4       GGAGATTGGCCCTTGGAGCATCGAAAAGTCAATGGAACCCCTAGCCCCATACCTATCATT
                  ************************************************************

HsCD00515866      TCATGGTGTGGCTCTCTGGATTCAAGAGATGTTGTCCTTCCAACGTATGACATCACCCAC
NM_153705.4       TCATGGTGTGGCTCTCTGGATTCAAGAGATGTTGTCCTTCCAACGTATGACATCACCCAC
                  ************************************************************

HsCD00515866      TCCATGCTTGAAGCCATGCGGGGTGTTACAAATGATCTCCTCTCTATTCAGGGAAATACA
NM_153705.4       TCCATGCTTGAAGCCATGCGGGGTGTTACAAATGATCTCCTCTCTATTCAGGGAAATACA
                  ************************************************************

HsCD00515866      GGGCCTTCCTGGATCAATAAAACAGAGAGAGCTTTCTTCAGAGGTAGAGACAGCCTAGAG
NM_153705.4       GGGCCTTCCTGGATCAATAAAACAGAGAGAGCTTTCTTCAGAGGTAGAGACAGCCGAGAG
                  ******************************************************* ****

HsCD00515866      GAGAGGCTCCAGTTGGTACAGCTGTCCAAAGAAAATCCTCAGCTACTAGATGCAGGAATT
NM_153705.4       GAGAGGCTCCAGTTGGTACAGCTGTCCAAAGAAAATCCTCAGCTACTAGATGCAGGAATT
                  ************************************************************

HsCD00515866      ACAGGATATTTCTTTTTCCAAGAGAAAGAAAAGGAGCTTGGAAAAGCCAAGTTGATGGGT
NM_153705.4       ACAGGATATTTCTTTTTCCAAGAGAAAGAAAAGGAGCTTGGAAAAGCCAAGTTGATGGGT
                  ************************************************************

HsCD00515866      TTCTTTGATTTCTTTAAGTACAAGTATCAAGTAAATGTGGATGGGACCGTGGCTGCTTAC
NM_153705.4       TTCTTTGATTTCTTTAAGTACAAGTATCAAGTAAATGTGGATGGGACCGTGGCTGCTTAC
                  ************************************************************

HsCD00515866      AGATATCCATATCTCATGCTGGGCGACAGTCTGGTTTTAAAGCAGGACTCGCCATATTAT
NM_153705.4       AGATATCCATATCTCATGCTGGGCGACAGTCTGGTTTTAAAGCAGGACTCGCCATATTAT
                  ************************************************************

HsCD00515866      GAACATTTCTACATGGCACTAGAACCTTGGAAGCATTATGTTCCAATTAAAAGAAATCTG
NM_153705.4       GAACATTTCTACATGGCACTAGAACCTTGGAAGCATTATGTTCCAATTAAAAGAAATCTG
                  ************************************************************

HsCD00515866      AGTGATTTATTAGAGAAAGTTAAATGGGCTAAGGAAAATGATGAAGAAGCCAAGAAGATT
NM_153705.4       AGTGATTTATTAGAGAAAGTTAAATGGGCTAAGGAAAATGATGAAGAAGCCAAGAAGATT
                  ************************************************************

HsCD00515866      GCAAAAGAAGGACAGTTGATGGCTAGGGACCTACTACAGCCACACAGGCTTTACTGCTAC
NM_153705.4       GCAAAAGAAGGACAGTTGATGGCTAGGGACCTACTACAGCCACACAGGCTTTACTGCTAC
                  ************************************************************

HsCD00515866      TATTACCAAGTACTGCAGAAATATGCCGAGCGCCAGTCCAGCAAACCCGAAGTACGTGAT
NM_153705.4       TATTACCAAGTACTGCAGAAATATGCCGAGCGCCAGTCCAGCAAACCCGAAGTACGTGAT
                  ************************************************************

HsCD00515866      GGAATGGAACTTGTTCCTCAGCCAGAAGATAGCACAGCCATCTGCCAGTGCCACAGGAAA
NM_153705.4       GGAATGGAACTTGTTCCTCAGCCAGAAGATAGCACAGCCATCTGCCAGTGCCACAGGAAA
                  ************************************************************

HsCD00515866      AAGCCTTCAAGAGAAGAACTT---
NM_153705.4       AAGCCTTCAAGAGAAGAACTTTGA
                  *********************