DNASU Plasmid | RALGPS2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RALGPS2

Gene Name: Ral GEF with PH domain and SH3 binding motif 2

Original Clone ID:

ccsbBroadEn_03523

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC047391.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515961

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1750nts

Open reading frame: 1 to 1750

Reference Sequence Alignment

ATGGACCTAATGAACGGGCAGGCAAGCAGTGTCAATATTGCAGCTACTGCTTCTGAGAAA
AGTAGCAGCTCTGAATCCTTAAGTGACAAAGGCTCTGAATTGAAGAAAAGCTTTGATGCT
GTGGTATTCGATGTTCTTAAGGTTACACCAGAAGAATATGCGGGTCAGATAACATTAATG
GATGTTCCAGTATTTAAAGCTATTCAACCAGATGAGCTTTCAAGTTGTGGATGGAATAAA
AAAGAAAAATATAGTTCTGCACCAAATGCAGTTGCCTTCACAAGAAGATTCAATCATGTA
AGCTTTTGGGTTGTTAGAGAGATTCTTCATGCTCAAACATTAAAAATTAGAGCAGAAGTT
TTGAGCCACTATATTAAAACTGCTAAGAAACTGTATGAGCTGAATAACCTTCATGCACTT
ATGGCAGTGGTTTCTGGCCTACAGAGTGCCCCAATTTTCAGGTTGACTAAAACATGGGCG
TTATTAAGTCGAAAAGACAAAACTACCTTTGAAAAATTAGAATATGTAATGAGTAAAGAA
GATAACTACAAAAGACTCAGAGACTATATAAGTAGCTTAAAGATGACACCTTGCATTCCC
TATTTAGGTATCTATTTGTCAGATTTAACATACATCGATTCAGCATACCCATCAACTGGC
AGCATTCTAGAAAATGAGCAAAGATCAAATTTAATGAATAATATCCTTCGAATAATTTCT
GATTTACAGCAGTCTTGTGAATATGATATTCCCATGTTGCCTCATGTCCAAAAATATCTC
AACTCTGTTCAGTATATAGAAGAACTACAAAAATTTGTGGAAGACGATAATTACAAGCTT
TCATTAAAGATAGAACCAGGGACAAGCACCCCACGTTCTGCTGCTTCCAGAGAAGATTTA
GTAGGTCCTGAAGTAGGAGCGTCTCCACAGAGTGGACGAAAAAGTGTGGCAGCTGAAGGA
GCCTTGCTCCCACAGACACCGCCATCCCCTCGGAATCTGATTCCACATGGACATAGGAAG
TGCCATAGTTTGGGTTATAATTTCATTCATAAAATGAACACAGCAGAATTTAAGAGTGCA
ACGTTTCCAAATGCAGGACCAAGACATCTGTTAGATGATAGCGTCATGGAGCCCCATGCG
CCATCTCGAGGCCAAGCTGAAAGTTCTACTCTTTCTAGTGGAATATCAATAGGTAGCAGC
GATGGTTCTGAACTAAGTGAAGAGACCTCATGGCCTGCTTTTGAAAGGAACAGATTATAC
CATTCTCTCGGCCCGGTGACAAGAGTGGCACGAAATGGCTATCGAAGTCACATGAAGGCC
AGCAGTTCTGCAGAATCAGAAGATTTGGCAGTACATTTATATCCAGGAGCTGTTACTATT
CAAGGTGTTCTCAGGAGAAAAACTTTGTTAAAAGAAGGCAAAAAGCCTACAGTAGCATCT
TGGACAAAATATTGGGCAGCTTTGTGTGGGACACAGCTTTTTTACTATGCTGCCAAATCT
CTAAAGGCTACCGAAAGAAAACATTTCAAATCAACATCCAATAAGAACGTATCTGTGATA
GGATGGATGGTGATGATGGCTGATGACCCTGAACATCCTGATCTCTTCCTGCTGACTGAC
TCTGAGAAAGGAAATTCGTACAAGTTTCAAGCTGGCAATAGAATGAATGCAATGTTATGG
TTTAAGCATTTGAGTGCAGCCTGCCAAAGTAACAAACAACAGGTTCCTACAAACTTGATG
ACTTTTGAG

Protein sequence predicted based on nucleotide sequence

MDLMNGQASSVNIAATASEKSSSSESLSDKGSELKKSFDAVVFDVLKVTPEEYAGQITLM
DVPVFKAIQPDELSSCGWNKKEKYSSAPNAVAFTRRFNHVSFWVVREILHAQTLKIRAEV
LSHYIKTAKKLYELNNLHALMAVVSGLQSAPIFRLTKTWALLSRKDKTTFEKLEYVMSKE
DNYKRLRDYISSLKMTPCIPYLGIYLSDLTYIDSAYPSTGSILENEQRSNLMNNILRIIS
DLQQSCEYDIPMLPHVQKYLNSVQYIEELQKFVEDDNYKLSLKIEPGTSTPRSAASREDL
VGPEVGASPQSGRKSVAAEGALLPQTPPSPRNLIPHGHRKCHSLGYNFIHKMNTAEFKSA
TFPNAGPRHLLDDSVMEPHAPSRGQAESSTLSSGISIGSSDGSELSEETSWPAFERNRLY
HSLGPVTRVARNGYRSHMKASSSAESEDLAVHLYPGAVTIQGVLRRKTLLKEGKKPTVAS
WTKYWAALCGTQLFYYAAKSLKATERKHFKSTSNKNVSVIGWMVMMADDPEHPDLFLLTD
SEKGNSYKFQAGNRMNAMLWFKHLSAACQSNKQQVPTNLMTFE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RALGPS2

Symbol Nomenclature :

RALGPS2

SYNONYM :

dJ595C2.1

Designation :

Ral-A exchange factor RalGPS2|ral GEF with PH domain and SH3-binding motif 2|ralA exchange factor RalGPS2|ras-specific guanine nucleotide-releasing factor RalGPS2

Full Nomenclature :

Ral GEF with PH domain and SH3 binding motif 2

GENEID :

55103

Locus Tag :

RP4-595C2.1

HGNC :

30279

Vega :

OTTHUMG00000035076

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515961        ATGGACCTAATGAACGGGCAGGCAAGCAGTGTCAATATTGCAGCTACTGCTTCTGAGAAA
NM_152663.4         ATGGACCTAATGAACGGGCAGGCAAGCAGTGTCAATATTGCAGCTACTGCTTCTGAGAAA
NM_001286247.1      ATGGACCTAATGAACGGGCAGGCAAGCAGTGTCAATATTGCAGCTACTGCTTCTGAGAAA
                    ************************************************************

HsCD00515961        AGTAGCAGCTCTGAATCCTTAAGTGACAAAGGCTCTGAATTGAAGAAAAGCTTTGATGCT
NM_152663.4         AGTAGCAGCTCTGAATCCTTAAGTGACAAAGGCTCTGAATTGAAGAAAAGCTTTGATGCT
NM_001286247.1      AGTAGCAGCTCTGAATCCTTAAGTGACAAAGGCTCTGAATTGAAGAAAAGCTTTGATGCT
                    ************************************************************

HsCD00515961        GTGGTATTCGATGTTCTTAAGGTTACACCAGAAGAATATGCGGGTCAGATAACATTAATG
NM_152663.4         GTGGTATTCGATGTTCTTAAGGTTACACCAGAAGAATATGCGGGTCAGATAACATTAATG
NM_001286247.1      GTGGTATTCGATGTTCTTAAGGTTACACCAGAAGAATATGCGGGTCAGATAACATTAATG
                    ************************************************************

HsCD00515961        GATGTTCCAGTATTTAAAGCTATTCAACCAGATGAGCTTTCAAGTTGTGGATGGAATAAA
NM_152663.4         GATGTTCCAGTATTTAAAGCTATTCAACCAGATGAGCTTTCAAGTTGTGGATGGAATAAA
NM_001286247.1      GATGTTCCAGTATTTAAAGCTATTCAACCAGATGAGCTTTCAAGTTGTGGATGGAATAAA
                    ************************************************************

HsCD00515961        AAAGAAAAATATAGTTCTGCACCAAATGCAGTTGCCTTCACAAGAAGATTCAATCATGTA
NM_152663.4         AAAGAAAAATATAGTTCTGCACCAAATGCAGTTGCCTTCACAAGAAGATTCAATCATGTA
NM_001286247.1      AAAGAAAAATATAGTTCTGCACCAAATGCAGTTGCCTTCACAAGAAGATTCAATCATGTA
                    ************************************************************

HsCD00515961        AGCTTTTGGGTTGTTAGAGAGATTCTTCATGCTCAAACATTAAAAATTAGAGCAGAAGTT
NM_152663.4         AGCTTTTGGGTTGTTAGAGAGATTCTTCATGCTCAAACATTAAAAATTAGAGCAGAAGTT
NM_001286247.1      AGCTTTTGGGTTGTTAGAGAGATTCTTCATGCTCAAACATTAAAAATTAGAGCAGAAGTT
                    ************************************************************

HsCD00515961        TTGAGCCACTATATTAAAACTGCTAAGAAACTGTATGAGCTGAATAACCTTCATGCACTT
NM_152663.4         TTGAGCCACTATATTAAAACTGCTAAGAAACTGTATGAGCTGAATAACCTTCATGCACTT
NM_001286247.1      TTGAGCCACTATATTAAAACTGCTAAGAAACTGTATGAGCTGAATAACCTTCATGCACTT
                    ************************************************************

HsCD00515961        ATGGCAGTGGTTTCTGGCCTACAGAGTGCCCCAATTTTCAGGTTGACTAAAACATGGGCG
NM_152663.4         ATGGCAGTGGTTTCTGGCCTACAGAGTGCCCCAATTTTCAGGTTGACTAAAACATGGGCG
NM_001286247.1      ATGGCAGTGGTTTCTGGCCTACAGAGTGCCCCAATTTTCAGGTTGACTAAAACATGGGCG
                    ************************************************************

HsCD00515961        TTATTAAGTCGAAAAGACAAAACTACCTTTGAAAAATTAGAATATGTAATGAGTAAAGAA
NM_152663.4         TTATTAAGTCGAAAAGACAAAACTACCTTTGAAAAATTAGAATATGTAATGAGTAAAGAA
NM_001286247.1      TTATTAAGTCGAAAAGACAAAACTACCTTTGAAAAATTAGAATATGTAATGAGTAAAGAA
                    ************************************************************

HsCD00515961        GATAACTACAAAAGACTCAGAGACTATATAAGTAGCTTAAAGATGACACCTTGCATTCCC
NM_152663.4         GATAACTACAAAAGACTCAGAGACTATATAAGTAGCTTAAAGATGACACCTTGCATTCCC
NM_001286247.1      GATAACTACAAAAGACTCAGAGACTATATAAGTAGCTTAAAGATGACACCTTGCATTCCC
                    ************************************************************

HsCD00515961        TATTTAGGTATCTATTTGTCAGATTTAACATACATCGATTCAGCATACCCATCAACTGGC
NM_152663.4         TATTTAGGTATCTATTTGTCAGATTTAACATACATCGATTCAGCATACCCATCAACTGGC
NM_001286247.1      TATTTAGGTATCTATTTGTCAGATTTAACATACATCGATTCAGCATACCCATCAACTGGC
                    ************************************************************

HsCD00515961        AGCATTCTAGAAAATGAGCAAAGATCAAATTTAATGAATAATATCCTTCGAATAATTTCT
NM_152663.4         AGCATTCTAGAAAATGAGCAAAGATCAAATTTAATGAATAATATCCTTCGAATAATTTCT
NM_001286247.1      AGCATTCTAGAAAATGAGCAAAGATCAAATTTAATGAATAATATCCTTCGAATAATTTCT
                    ************************************************************

HsCD00515961        GATTTACAGCAGTCTTGTGAATATGATATTCCCATGTTGCCTCATGTCCAAAAATATCTC
NM_152663.4         GATTTACAGCAGTCTTGTGAATATGATATTCCCATGTTGCCTCATGTCCAAAAATATCTC
NM_001286247.1      GATTTACAGCAGTCTTGTGAATATGATATTCCCATGTTGCCTCATGTCCAAAAATATCTC
                    ************************************************************

HsCD00515961        AACTCTGTTCAGTATATAGAAGAACTACAAAAATTTGTGGAAGACGATAATTACAAGCTT
NM_152663.4         AACTCTGTTCAGTATATAGAAGAACTACAAAAATTTGTGGAAGACGATAATTACAAGCTT
NM_001286247.1      AACTCTGTTCAGTATATAGAAGAACTACAAAAATTTGTGGAAGACGATAATTACAAGCTT
                    ************************************************************

HsCD00515961        TCATTAAAGATAGAACCAGGGACAAGCACCCCACGTTCTGCTGCTTCCAGAGAAGATTTA
NM_152663.4         TCATTAAAGATAGAACCAGGGACAAGCACCCCACGTTCTGCTGCTTCCAGAGAAGATTTA
NM_001286247.1      TCATTAAAGATAGAACCAGGGACAAGCACCCCACGTTCTGCTGCTTCCAGAGAAGATTTA
                    ************************************************************

HsCD00515961        GTAGGTCCTGAAGTAGGAGCGTCTCCACAGAGTGGACGAAAAAGTGTGGCAGCTGAAGGA
NM_152663.4         GTAGGTCCTGAAGTAGGAGCGTCTCCACAGAGTGGACGAAAAAGTGTGGCAGCTGAAGGA
NM_001286247.1      GTAGGTCCTGAAGTAGGAGCGTCTCCACAGAGTGGACGAAAAAGTGTGGCAGCTGAAGGA
                    ************************************************************

HsCD00515961        GCCTTGCTCCCACAGACACCGCCATCCCCTCGGAATCTGATTCCACATGGACATAGGAAG
NM_152663.4         GCCTTGCTCCCACAGACACCGCCATCCCCTCGGAATCTGATTCCACATGGACATAGGAAG
NM_001286247.1      GCCTTGCTCCCACAGACACCGCCATCCCCTCGGAATCTGATTCCACATGGACATAGGAAG
                    ************************************************************

HsCD00515961        TGCCATAGTTTGGGTTATAATTTCATTCATAAAATGAACACAGCAGAATTTAAGAGTGCA
NM_152663.4         TGCCATAGTTTGGGTTATAATTTCATTCATAAAATGAACACAGCAGAATTTAAGAGTGCA
NM_001286247.1      TGCCATAGTTTGGGTTATAATTTCATTCATAAAATGAACACAGCAGAATTTAAGAGTGCA
                    ************************************************************

HsCD00515961        ACGTTTCCAAATGCAGGACCAAGACATCTGTTAGATGATAGCGTCATGGAGCCCCATGCG
NM_152663.4         ACGTTTCCAAATGCAGGACCAAGACATCTGTTAGATGATAGCGTCATGGAGCCCCATGCG
NM_001286247.1      ACGTTTCCAAATGCAGGACCAAGACATCTGTTAGATGATAGCGTCATGGAGCCCCATGCG
                    ************************************************************

HsCD00515961        CCATCTCGAGGCCAAGCTGAAAGTTCTACTCTTTCTAGTGGAATATCAATAGGTAGCAGC
NM_152663.4         CCATCTCGAGGCCAAGCTGAAAGTTCTACTCTTTCTAGTGGAATATCAATAGGTAGCAGC
NM_001286247.1      CCATCTCGAGGCCAAGCTGAAAGTTCTACTCTTTCTAGTGGAATATCAATAGGTAGCAGC
                    ************************************************************

HsCD00515961        GATGGTTCTGAACTAAGTGAAGAGACCTCATGGCCTGCTTTTGAAAGGAACAGATTATAC
NM_152663.4         GATGGTTCTGAACTAAGTGAAGAGACCTCATGGCCTGCTTTTGAAAGGAACAGATTATAC
NM_001286247.1      GATGGTTCTGAACTAAGTGAAGAGACCTCATGGCCTGCTTTTGAAAG-------------
                    ***********************************************             

HsCD00515961        CATTCTCTCGGCCCGGTGACAAGAGTGGCACGAAATGGCTATCGAAGTCACATGAAGGCC
NM_152663.4         CATTCTCTCGGCCCGGTGACAAGAGTGGCACGAAATGGCTATCGAAGTCACATGAAGGCC
NM_001286247.1      ------------------------------------------------------------
                                                                                

HsCD00515961        AGCAGTTCTGCAGAATCAGAAGATTTGGCAGTACATTTATATCCAGGAGCTGTTACTATT
NM_152663.4         AGCAGTTCTGCAGAATCAGAAGATTTGGCAGTACATTTATATCCAGGAGCTGTTACTATT
NM_001286247.1      -----TTCTGCAGAATCAGAAGATTTGGCAGTACATTTATATCCAGGAGCTGTTACTATT
                         *******************************************************

HsCD00515961        CAAGGTGTTCTCAGGAGAAAAACTTTGTTAAAAGAAGGCAAAAAGCCTACAGTAGCATCT
NM_152663.4         CAAGGTGTTCTCAGGAGAAAAACTTTGTTAAAAGAAGGCAAAAAGCCTACAGTAGCATCT
NM_001286247.1      CAAGGTGTTCTCAGGAGAAAAACTTTGTTAAAAGAAGGCAAAAAGCCTACAGTAGCATCT
                    ************************************************************

HsCD00515961        TGGACAAAATATTGGGCAGCTTTGTGTGGGACACAGCTTTTTTACTATGCTGCCAAATCT
NM_152663.4         TGGACAAAATATTGGGCAGCTTTGTGTGGGACACAGCTTTTTTACTATGCTGCCAAATCT
NM_001286247.1      TGGACAAAATATTGGGCAGCTTTGTGTGGGACACAGCTTTTTTACTATGCTGCCAAATCT
                    ************************************************************

HsCD00515961        CTAAAGGCTACCGAAAGAAAACATTTCAAATCAACATCCAATAAGAACGTATCTGTGATA
NM_152663.4         CTAAAGGCTACCGAAAGAAAACATTTCAAATCAACATCCAATAAGAACGTATCTGTGATA
NM_001286247.1      CTAAAGGCTACCGAAAGAAAACATTTCAAATCAACATCCAATAAGAACGTATCTGTGATA
                    ************************************************************

HsCD00515961        GGATGGATGGTGATGATGGCTGATGACCCTGAACATCCTGATCTCTTCCTGCTGACTGAC
NM_152663.4         GGATGGATGGTGATGATGGCTGATGACCCTGAACATCCTGATCTCTTCCTGCTGACTGAC
NM_001286247.1      GGATGGATGGTGATGATGGCTGATGACCCTGAACATCCTGATCTCTTCCTGCTGACTGAC
                    ************************************************************

HsCD00515961        TCTGAGAAAGGAAATTCGTACAAGTTTCAAGCTGGCAATAGAATGAATGCAATGTTATGG
NM_152663.4         TCTGAGAAAGGAAATTCGTACAAGTTTCAAGCTGGCAATAGAATGAATGCAATGTTATGG
NM_001286247.1      TCTGAGAAAGGAAATTCGTACAAGTTTCAAGCTGGCAATAGAATGAATGCAATGTTATGG
                    ************************************************************

HsCD00515961        TTTAAGCATTTGAGTGCAGCCTGCCAAAGTAACAAACAACAGGTTCCTACAAACTTGATG
NM_152663.4         TTTAAGCATTTGAGTGCAGCCTGCCAAAGTAACAAACAACAGGTTCCTACAAACTTGATG
NM_001286247.1      TTTAAGCATTTGAGTGCAGCCTGCCAAAGTAACAAACAACAGGTTCCTACAAACTTGATG
                    ************************************************************

HsCD00515961        ACTTTTGAG---
NM_152663.4         ACTTTTGAGTAG
NM_001286247.1      ACTTTTGAGTAG
                    *********