Gene:
Gene Symbol:
SSH1
Gene Name:
slingshot protein phosphatase 1
Original Clone ID:
ccsbBroadEn_08375
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC062341.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516056
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGTCCTGGTGACCCTGCAGCGCTCGCCCACGCCCAGCGCCGCCTCCTCCTCGGCCAGC
AACAGCGAGTTGGAGGCTGGCAGCGAAGAAGATCGAAAATTAAACCTCAGCTTAAGTGAG
AGCTTTTTCATGGTGAAAGGCGCAGCCCTCTTCTTACAACAGGGAAGCAGCCCTCAAGGC
CAGCGGAGTCTTCAGCACCCCCACAAGCATGCAGGTGATCTGCCTCAACATCTTCAGGTG
ATGATCAACCTTCTGCGTTGCGAAGACAGAATCAAGCTGGCAGTGCGCCTGGAGAGCGCC
TGGGCGGACCGGGTCCGGTACATGGTGGTGGTGTACAGCAGCGGGCGCCAGGACACCGAG
GAGAATATCTTGCTGGGAGTGGACTTTTCCAGTAAGGAAAGTAAAAGCTGCACCATTGGG
ATGGTTCTCCGACTGTGGAGCGACACGAAAATCCACCTTGATGGAGATGGTGGGTTCAGC
GTGAGCACAGCAGGAAGGATGCACATATTTAAGCCTGTGTCTGTCCAGGCCATGTGGTCT
GCCCTGCAGGTGCTTCACAAGGCCTGCGAAGTGGCCCGGAGGCACAACTACTTCCCCGGG
GGTGTAGCTCTCATCTGGGCTACCTACTATGAGAGCTGCATCAGCTCCGAGCAGAGCTGC
ATCAACGAGTGGAACGCCATGCAGGACCTGGAGTCTACGCGGCCCGACTCCCCCGCGCTA
TTTGTGGACAAGCCCACTGAAGGGGAAAGGACCGAGCGCCTCATCAAAGCCAAGCTCCGA
AGCATCATGATGAGCCAGGATCTAGAAAATGTGACTTCCAAAGAGATTCGTAATGAATTA
GAGAAACAGATGAATTGTAACTTGAAGGAACTCAAGGAATTTATAGACAATGAGATGCTA
CTTATCTTGGGACAGATGGACAAGCCCTCCCTTATCTTCGATCATCTTTATCTCGGCTCT
GAATGGAATGCATCCAATCTGGAGGAACTGCAGGGCTCAGGGGTTGATTACATTTTAAAT
GTTACCAGAGAAATCGATAATTTTTTTCCTGGCTTATTTGCATATCATAACATCCGAGTC
TACGATGAAGAGACCACAGACCTCCTCGCCCACTGGAATGAAGCGTATCATTTTATAAAC
AAAGCGAAGAGGAACCATTCCAAGTGCCTGGTGCATTGCAAAATGGGCGTGAGTCGCTCG
GCCTCCACAGTCATAGCCTATGCAATGAAGGAATTCGGCTGGCCTCTGGAAAAAGCATAT
AACTATGTAAAGCAGAAGCGCAGCATCACGCGCCCCAACGCGGGCTTTATGAGGCAGCTG
TCTGAGTATGAAGGCATCTTGGATGCAAGCAAACAGCGGCACAACAAGCTGTGGCGTCAG
CAGACAGACAGCAGCCTCCAGCAGCCTGTGGATGACCCTGCAGGACCTGGCGACTTCTTG
CCAGAGACCCCAGATGGCACCCCGGAAAGCCAGCTGCCCTTCTTGGATGATGCCGCCCAG
CCCGGCTTAGGGCCCCCCCTCCCCTGCTGTTTCCGGCGACTCTCAGACCCCCTTCTGCCT
TCCCCTGAGGATGAAACTGGCAGCTTGGTCCACCTGGAGGATCCGGAGAGGGAGGCTCTG
TTGGAGGAAGCTGCTCCACCTGCAGAGGTGCACAGGCCGGCCAGACAGCCCCAGCAAGGT
TCCGGACTCTGTGAGAAGGATGTGAAGAAGAAACTAGAGTTTGGGAGTCCCAAAGGTCGG
AGCGGCTCCTTGCTGCAGGTGGAGGAGACGGAAAGGGAGGAGGGCCTGGGAGCAGGGAGG
TGGGGGCAGCTTCCAACCCAGCTCGATCAAAACCTGCTCAACTCGGAGAACCTAAACAAC
AACAGCAAGAGGAGCTGTCCCAACGGCATGGAGGATGATGCTATATTTGGGATCCTTAAC
AAAGTGAAGCCTTCCTATAAATCCTGTGCCGACTGCATGTACCCTACAGCCAGCGGGGCT
CCTGAGGCCTCCAGGGAGCGATGTGAGGACCCCAATGCTCCCGCCATCTGCACCCAGCCA
GCCTTCCTACCCCACATCACGTCCTCCCCTGTGGCCCACTTGGCCAGCAGGTCCCGTGTT
CCGGAGAAGCCAGCCTCTGGCCCAACCGAACCTCCCCCGTTCCTACCACCAGCAGGCTCC
AGGAGGGCAGACACCAGTGGCCCTGGGGCTGGAGCTGCCCTAGAGCCACCAGCCAGCCTT
TTGGAACCTTCCAGAGAGACCCCAAAAGTCCTGCCAAAGTCCCTCCTTTTGAAGAATTCT
CACTGTGATAAGAACCCTCCCAGCACAGAAGTGGTAATAAAGGAAGAATCGTCACCCAAG
AAAGATATGAAGCCAGCCAAGGACCTGAGGCTTCTGTTCAGTAATGAATCTGAGAAGCCG
ACAACCAACAGCTACCTGATGCAGCACCAGGAGTCCATCATTCAGCTGCAGAAGGCAGGC
TTGGTCCGCAAGCACACCAAAGAGCTAGAGCGGCTGAAGAGCGTGCCTGCAGACCCAGCA
CCTCCCTCCAGGGATGGCCCTGCCAGCAGGCTGGAGGCCAGCATCCCCGAGGAGAGCCAG
GATCCAGCCGCGCTCCACGAGCTGGGCCCCCTGGTTATGCCCAGCCAGGCCGGGAGTGAT
GAGAAGTCAGAGGCCGCCCCCGCTTCATTGGAAGGAGGCTCACTGAAGAGCCCCCCTCCT
TTCTTCTACCGCCTGGACCACACCAGTAGTTTCTCAAAAGACTTTCTGAAGACCATCTGC
TACACCCCCACCTCCTCTTCCATGAGCTCCAACCTGACCCGGAGTTCCAGCAGCGATAGC
ATCCACAGTGTCCGTGGGAAGCCCGGGCTGGTGAAGCAGCGGACGCAGGAGATTGAGACC
CGGCTCCGGCTGGCGGGCCTCACCGTCTCTTCCCCACTGAAGCGCTCACACTCTCTTGCC
AAGCTGGGGAGTCTCACCTTCTCAACGGAAGACCTGTCCAGTGAGGCTGACCCGTCCACC
GTCGCTGACTCCCAGGACACCACTTTGAGTGAATCTTCCTTCTTGCATGAGCCCCAGGGA
ACCCCGAGGGACCCAGCTGCAACCTCCAAACCATCAGGGAAACCCGCCCCAGAAAACTTA
AAGAGCCCTTCGTGGATGAGCAAAAGC
Protein sequence predicted based on nucleotide sequence
MVLVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFMVKGAALFLQQGSSPQG QRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTE ENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWS ALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPAL FVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKELKEFIDNEML LILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRV YDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAY NYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDFL PETPDGTPESQLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREAL LEEAAPPAEVHRPARQPQQGSGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGR WGQLPTQLDQNLLNSENLNNNSKRSCPNGMEDDAIFGILNKVKPSYKSCADCMYPTASGA PEASRERCEDPNAPAICTQPAFLPHITSSPVAHLASRSRVPEKPASGPTEPPPFLPPAGS RRADTSGPGAGAALEPPASLLEPSRETPKVLPKSLLLKNSHCDKNPPSTEVVIKEESSPK KDMKPAKDLRLLFSNESEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSVPADPA PPSRDGPASRLEASIPEESQDPAALHELGPLVMPSQAGSDEKSEAAPASLEGGSLKSPPP FFYRLDHTSSFSKDFLKTICYTPTSSSMSSNLTRSSSSDSIHSVRGKPGLVKQRTQEIET RLRLAGLTVSSPLKRSHSLAKLGSLTFSTEDLSSEADPSTVADSQDTTLSESSFLHEPQG TPRDPAATSKPSGKPAPENLKSPSWMSKS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
