PHF8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PHF8
Gene Name:  PHD finger protein 8
Original Clone ID:
ccsbBroadEn_02723
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC053861.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516076
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3072nts
Open reading frame: 1 to 3072

ATGGCCTCGGTGCCGGTGTATTGCCTCTGCCGGCTGCCTTACGATGTGACCCGCTTCATG
ATCGAGTGTGACATGTGCCAGGACTGGTTTCATGGCAGTTGTGTTGGTGTTGAAGAGGAG
AAGGCTGCTGACATTGACCTCTACCACTGCCCCAACTGTGAAGTCTTGCATGGGCCCTCC
ATTATGAAAAAACGCCGTGGATCTTCAAAGGGGCATGATACACACAAGGGGAAACCAGTG
AAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTAGGACTTTTGACAGCTCAGAT
GAAGTGATTCTGAAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGAAGAAAATAGC
TTCAGTGTGCCCATCCTGGTCCTGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCA
TCATTCACTGTGAGGGATGTTGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATT
GATGTGACCCGCCAGGCTGACTGCAAGATGAAGCTTGGTGATTTTGTGAAATACTATTAC
AGCGGGAAGAGGGAGAAAGTCCTCAATGTCATTAGTTTGGAATTCTCTGATACCAGACTT
TCTAACCTTGTGGAGACACCGAAGATTGTTCGAAAGCTGTCATGGGTCGAAAACTTGTGG
CCAGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGTACTGCCTCATGAGTGTGCGA
GATAGCTATACAGACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTACCATGTACTC
AAGGGTGAAAAGATCTTCTACCTGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAG
TGCTGGAGCAGTTCCTCTAATCAGAATGAGATGTTCTTTGGGGACCAGGTGGACAAGTGC
TACAAGTGTTCCGTGAAGCAAGGACAGACACTTTTCATTCCCACAGGGTGGATCCATGCT
GTGCTGACGCCTGTGGACTGCCTTGCCTTTGGAGGGAACTTCTTACACAGCCTTAACATC
GAGATGCAGCTCAAAGCCTATGAGATTGAGAAGCGGCTGAGCACAGCAGACCTCTTCAGA
TTCCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGCACATCCTGGACATCTTTCGC
GGTTTGCGAGAGAACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGGCAAAGCCTTG
AACTTGGCCTTTAGAGCCTGGACAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATC
CCGGAGACAGTGCGAACCGTACAGCTCATTAAAGATCTGGCCAGGGAGATCCGCCTGGTG
GAAGACATCTTCCAACAGAACGTTGGGAAGACGAGCAATATCTTTGGGCTGCAGAGGATC
TTCCCAGCCGGCTCCATTCCCCTAACCAGGCCAGCCCATTCCACTTCAGTGTCCATGTCC
AGGCTGTCACTGCCCTCCAAAAATGGTTCAAAGAAGAAAGGCCTGAAGCCCAAGGAACTC
TTCAAGAAGGCAGAGCGAAAGGGCAAGGAGAGTTCAGCCTTGGGGCCTGCTGGCCAGTTG
AGCTATAATCTCATGGACACATACAGTCATCAGGCACTGAAGACAGGCTCTTTCCAGAAA
GCAAAGTTCAACATCACTGGTGCCTGCTTGAATGACTCAGATGACGACTCACCAGACTTG
GACCTTGATGGAAATGAGAGCCCATTGGCCCTATTGATGTCTAACGGCAGTACGAAAAGG
GTGAAGAGTTTATCCAAATCTCGGCGAACCAAGATAGCAAAGAAGGTAGACAAGGCTAGG
CTGATGGCAGAACAGGTGATGGAAGACGAATTTGACTTGGATTCAGATGATGAGCTGCAG
ATTGACGAGAGATTGGGAAAGGAGAAGGCGACCCTGATAATAAGACCAAAATTTCCCCGG
AAATTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAGTTCGTGAACCAGGAGAAGTT
GAGTTTGACATTGAGGAGGACTATACAACAGATGAGGACATGGTGGAAGGGGTTGAAGGC
AAGCTTGGGAATGGTAGTGGCGCTGGTGGCATTCTTGATCTGCTCAAGGCCAGCAGGCAG
GTGGGGGGACCTGACTATGCTGCCCTCACCGAGGCCCCAGCTTCTCCCAGCACTCAGGAG
GCCATCCAGGGCATGCTGTGCATGGCCAACCTGCAGTCCTCATCGTCCTCACCGGCTACC
TCTAGCCTGCAGGCCTGGTGGACTGGGGGACAGGATCGAAGCAGTGGGAGCTCCAGCAGT
GGGCTGGGCACAGTGTCTAACAGTCCTGCTTCCCAGCGCACCCCAGGGAAGCGGCCCATC
AAGCGGCCAGCATACTGGAGAACCGAGAGCGAGGAGGAGGAGGAGAACGCCAGTCTGGAT
GAACAGGACAGCTTGGGAGCGTGCTTCAAGGATGCAGAGTATATCTATCCTTCACTGGAG
TCTGATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGAT
GCTCCATGGAGTCCTAAAGCCCGCGTGACCCCAACTCTGCCGAAGCAGGACCGTCCTGTG
CGTGAGGGGACCCGGGTAGCCTCTATTGAGACAGGTTTGGCTGCAGCAGCTGCAAAGCTG
GCCCAGCAGGAGCTACAGAAGGCCCAAAAGAAGAAATATATCAAGAAGAAGCCTTTGCTG
AAGGAGGTAGAACAGCCTCGCCCTCAAGACTCCAATCTCAGTCTGACAGTACCAGCCCCC
ACTGTGGCTGCCACACCACAACTTGTCACCTCCTCCTCACCCCTGCCTCCTCCTGAGCCT
AAACAAGAGGCCCTGTCAGGAAGTCTCGCTGACCATGAGTACACCGCTCGTCCCAATGCC
TTTGGCATGGCCCAGGCAAACCGCAGCACCACACCTATGGCCCCCGGTGTCTTCTTGACC
CAGCGGCGCCCTTCAGTTGGCTCCCAGAGCAATCAGGCAGGACAAGGAAAGCGTCCCAAA
AAGGGCCTGGCCACAGCAAAGCAGAGACTCGGCCGTATCCTGAAAATCCACAGAAATGGC
AAACTACTTCTG

Protein sequence predicted based on nucleotide sequence

MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPS
IMKKRRGSSKGHDTHKGKPVKTGSPTFVRELRSRTFDSSDEVILKPTGNQLTVEFLEENS
FSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVTRQADCKMKLGDFVKYYY
SGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVR
DSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVDKC
YKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFR
FPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEI
PETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMS
RLSLPSKNGSKKKGLKPKELFKKAERKGKESSALGPAGQLSYNLMDTYSHQALKTGSFQK
AKFNITGACLNDSDDDSPDLDLDGNESPLALLMSNGSTKRVKSLSKSRRTKIAKKVDKAR
LMAEQVMEDEFDLDSDDELQIDERLGKEKATLIIRPKFPRKLPRAKPCSDPNRVREPGEV
EFDIEEDYTTDEDMVEGVEGKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQE
AIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPASQRTPGKRPI
KRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDD
APWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLL
KEVEQPRPQDSNLSLTVPAPTVAATPQLVTSSSPLPPPEPKQEALSGSLADHEYTARPNA
FGMAQANRSTTPMAPGVFLTQRRPSVGSQSNQAGQGKRPKKGLATAKQRLGRILKIHRNG
KLLL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable