DNASU Plasmid | MYPOP (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MYPOP

Gene Name: Myb-related transcription factor, partner of profilin

Original Clone ID:

ccsbBroadEn_05457

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC044311.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516097

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1198nts

Open reading frame: 1 to 1198

Reference Sequence Alignment

ATGGCCTCGGCGGCGGCGGGCGAAGCGGAGGAAACCACCCGGTTGCGCAAGCCGCGCTTC
TCATTCGAAGAGAACCAGATCCTGATCCGCGAGGTGCGCGCCCACTACCCGCAGCTCTAC
GGCGCGCAGAGCCGTCGGGTGAGCGTGGCAGAGCGGCGGCGCGTGTGGGACGGCATCGCC
GCCAAGATCAACGGTATCACCAGCTGGAAGCGCACGGGCCAGGAGGTGCAGAAGCGCTGG
AACGACTTCAAGCGCCGCACCAAGGAGAAGCTCGCTCGCGTGCCGCACTCCACGCAGGGC
GCCGGGCCCGCCGCGGAGGACGCTTTCTCCGCGGAAGAGGAGACCATTTTTGCCATCCTG
GGGCCAGGTGTGGCGGCGCCGGGGGCAGGTGCTGGGGCGGAGGAGCCCCCTGCGGCCCCC
TCTTCACAGCCGCCGCCCCCAAGCGCCTGCCCCCAGCGCTACGTGTTGTCGGAAGACCGC
CGGGAGGACCGACGTGCAGATACATCAGCCCACAGCAAGGCGGGCTCCAGCAGCCCGGAG
CCATGGGCCCGGCCCTCCTGCACTCCCCAGGAAGGGGGCTGCCCACGGCCCAAGGAGCGT
GAGTCACCACCCCCTTCGGCCCTGCAGCCGGTCCAGCTGCCTCGCCTGGCCTTGTCTCCA
CCACCCCCAGCCCCTCCACTGCCACCCCCACCGCCACTGGCCCAAGTGGCACCCTCACCC
CCTAGCCCCCCACCCCCTCCTCGGCCTCCACCCACGCTCTCGGCCTCAGACCCCTCCCTG
GACTTCCTGCGGGCCCAGCAGGAGACTGCCAACGCCATCCGGGAGCTGGCCGGCACCCTT
CGACAGGGACTGGCCAAACTGAGCGAGGCCCTCAGCGCTCTGCTGCCCCTTCTACCAGGA
ACCCCAGTTGACTCCCTGCCTCCACCTCTGCCCCCACCCCCACCCCCACCGCCACCTCCC
AGGCCTGTCCTGCCCCCACCGGCCCCCAAGGTGGAGATCACCCCAGAGCCCGTGTCCGTG
GTGGCTGCTGTGGTGGACGGGGCAGTGGTGGCAGCCAGGGGAGTGATCATTGCCCCAAGG
AGCGAGGAGGGGGCACCCCGGCCCCCCCCAGCCCCGCTCCCTCCGCACGACTCCCCCCCA
CACAAGCGGAGAAAAGGTTTCCCTACACGGAAAAGGCGCGGCCGATGGAAATCTCCG

Protein sequence predicted based on nucleotide sequence

MASAAAGEAEETTRLRKPRFSFEENQILIREVRAHYPQLYGAQSRRVSVAERRRVWDGIA
AKINGITSWKRTGQEVQKRWNDFKRRTKEKLARVPHSTQGAGPAAEDAFSAEEETIFAIL
GPGVAAPGAGAGAEEPPAAPSSQPPPPSACPQRYVLSEDRREDRRADTSAHSKAGSSSPE
PWARPSCTPQEGGCPRPKERESPPPSALQPVQLPRLALSPPPPAPPLPPPPPLAQVAPSP
PSPPPPPRPPPTLSASDPSLDFLRAQQETANAIRELAGTLRQGLAKLSEALSALLPLLPG
TPVDSLPPPLPPPPPPPPPPRPVLPPPAPKVEITPEPVSVVAAVVDGAVVAARGVIIAPR
SEEGAPRPPPAPLPPHDSPPHKRRKGFPTRKRRGRWKSP

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

MYPOP

Symbol Nomenclature :

MYPOP

SYNONYM :

P42pop

Designation :

myb-related protein p42POP|myb-related transcription factor, partner of profilin|p42 Myb-related transcription factor, partner of profilin|partner of profilin

Full Nomenclature :

Myb-related transcription factor, partner of profilin

GENEID :

339344

HGNC :

20178

Vega :

OTTHUMG00000182486

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516097        ATGGCCTCGGCGGCGGCGGGCGAAGCGGAGGAAACCACCCGGTTGCGCAAGCCGCGCTTC
NM_001012643.2      ATGGCCTCGGCGGCGGCGGGCGAAGCGGAGGAAACCACCCGGTTGCGCAAGCCGCGCTTC
                    ************************************************************

HsCD00516097        TCATTCGAAGAGAACCAGATCCTGATCCGCGAGGTGCGCGCCCACTACCCGCAGCTCTAC
NM_001012643.2      TCATTCGAAGAGAACCAGATCCTGATCCGCGAGGTGCGCGCCCACTACCCGCAGCTCTAC
                    ************************************************************

HsCD00516097        GGCGCGCAGAGCCGTCGGGTGAGCGTGGCAGAGCGGCGGCGCGTGTGGGACGGCATCGCC
NM_001012643.2      GGCGCGCAGAGCCGTCGGGTGAGCGTGGCAGAGCGGCGGCGCGTGTGGGACGGCATCGCC
                    ************************************************************

HsCD00516097        GCCAAGATCAACGGTATCACCAGCTGGAAGCGCACGGGCCAGGAGGTGCAGAAGCGCTGG
NM_001012643.2      GCCAAGATCAACGGTATCACCAGCTGGAAGCGCACGGGCCAGGAGGTGCAGAAGCGCTGG
                    ************************************************************

HsCD00516097        AACGACTTCAAGCGCCGCACCAAGGAGAAGCTCGCTCGCGTGCCGCACTCCACGCAGGGC
NM_001012643.2      AACGACTTCAAGCGCCGCACCAAGGAGAAGCTCGCTCGCGTGCCGCACTCCACGCAGGGC
                    ************************************************************

HsCD00516097        GCCGGGCCCGCCGCGGAGGACGCTTTCTCCGCGGAAGAGGAGACCATTTTTGCCATCCTG
NM_001012643.2      GCCGGGCCCGCCGCGGAGGACGCTTTCTCCGCGGAAGAGGAGACCATTTTTGCCATCCTG
                    ************************************************************

HsCD00516097        GGGCCAGGTGTGGCGGCGCCGGGGGCAGGTGCTGGGGCGGAGGAGCCCCCTGCGGCCCCC
NM_001012643.2      GGGCCAGGTGTGGCGGCGCCGGGGGCAGGTGCTGGGGCGGAGGAGCCCCCTGCGGCCCCC
                    ************************************************************

HsCD00516097        TCTTCACAGCCGCCGCCCCCAAGCGCCTGCCCCCAGCGCTACGTGTTGTCGGAAGACCGC
NM_001012643.2      TCTTCACAGCCGCCGCCCCCAAGCGCCTGCCCCCAGCGCTACGTGTTGTCGGAAGACCGC
                    ************************************************************

HsCD00516097        CGGGAGGACCGACGTGCAGATACATCAGCCCACAGCAAGGCGGGCTCCAGCAGCCCGGAG
NM_001012643.2      CGGGAGGACCGACGTGCAGATACATCAGCCCACAGCAAGGCGGGCTCCAGCAGCCCGGAG
                    ************************************************************

HsCD00516097        CCATGGGCCCGGCCCTCCTGCACTCCCCAGGAAGGGGGCTGCCCACGGCCCAAGGAGCGT
NM_001012643.2      CCATGGGCCCGGCCCTCCTGCACTCCCCAGGAAGGGGGCTGCCCACGGCCCAAGGAGCGT
                    ************************************************************

HsCD00516097        GAGTCACCACCCCCTTCGGCCCTGCAGCCGGTCCAGCTGCCTCGCCTGGCCTTGTCTCCA
NM_001012643.2      GAGTCACCACCCCCTTCGGCCCTGCAGCCGGTCCAGCTGCCTCGCCTGGCCTTGTCTCCA
                    ************************************************************

HsCD00516097        CCACCCCCAGCCCCTCCACTGCCACCCCCACCGCCACTGGCCCAAGTGGCACCCTCACCC
NM_001012643.2      CCACCCCCAGCCCCTCCACTGCCACCCCCACCGCCACTGGCCCAAGTGGCACCCTCACCC
                    ************************************************************

HsCD00516097        CCTAGCCCCCCACCCCCTCCTCGGCCTCCACCCACGCTCTCGGCCTCAGACCCCTCCCTG
NM_001012643.2      CCTAGCCCCCCACCCCCTCCTCGGCCTCCACCCACGCTCTCGGCCTCAGACCCCTCCCTG
                    ************************************************************

HsCD00516097        GACTTCCTGCGGGCCCAGCAGGAGACTGCCAACGCCATCCGGGAGCTGGCCGGCACCCTT
NM_001012643.2      GACTTCCTGCGGGCCCAGCAGGAGACTGCCAACGCCATCCGGGAGCTGGCCGGCACCCTT
                    ************************************************************

HsCD00516097        CGACAGGGACTGGCCAAACTGAGCGAGGCCCTCAGCGCTCTGCTGCCCCTTCTACCAGGA
NM_001012643.2      CGACAGGGACTGGCCAAACTGAGCGAGGCCCTCAGCGCTCTGCTGCCCCTTCTACCAGGA
                    ************************************************************

HsCD00516097        ACCCCAGTTGACTCCCTGCCTCCACCTCTGCCCCCACCCCCACCCCCACCGCCACCTCCC
NM_001012643.2      ACCCCAGTTGACTCCCTGCCTCCACCTCTGCCCCCACCCCCACCCCCACCGCCACCTCCC
                    ************************************************************

HsCD00516097        AGGCCTGTCCTGCCCCCACCGGCCCCCAAGGTGGAGATCACCCCAGAGCCCGTGTCCGTG
NM_001012643.2      AGGCCTGTCCTGCCCCCACCGGCCCCCAAGGTGGAGATCACCCCAGAGCCCGTGTCCGTG
                    ************************************************************

HsCD00516097        GTGGCTGCTGTGGTGGACGGGGCAGTGGTGGCAGCCAGGGGAGTGATCATTGCCCCAAGG
NM_001012643.2      GTGGCTGCTGTGGTGGACGGGGCAGTGGTGGCAGCCAGGGGAGTGATCATTGCCCCAAGG
                    ************************************************************

HsCD00516097        AGCGAGGAGGGGGCACCCCGGCCCCCCCCAGCCCCGCTCCCTCCGCACGACTCCCCCCCA
NM_001012643.2      AGCGAGGAGGGGGCACCCCGGCCCCCCCCAGCCCCGCTCCCTCCGCACGACTCCCCCCCA
                    ************************************************************

HsCD00516097        CACAAGCGGAGAAAAGGTTTCCCTACACGGAAAAGGCGCGGCCGATGGAAATCTCCG---
NM_001012643.2      CACAAGCGGAGAAAAGGTTTCCCTACACGGAAAAGGCGCGGCCGATGGAAATCTCCGTGA
                    *********************************************************