DNASU Plasmid | RPS6KB2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RPS6KB2

Gene Name: ribosomal protein S6 kinase, 70kDa, polypeptide 2

Original Clone ID:

ccsbBroadEn_06897

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC006106.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516119

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1447nts

Open reading frame: 1 to 1447

Reference Sequence Alignment

ATGGCGGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAGCGAGGGCGAGGGCGAG
CCAGAGCTCAGCCCCGCGGACGCATGTCCCCTTGCCGAGTTGAGGGCAGCTGGCCTAGAG
CCTGTGGGACACTATGAAGAGGTGGAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAG
CGCATCGGGCCCCACTCCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAG
GTGTTCCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATGAAAGTC
CTAAGGAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACACACACGGGCTGAGCGG
AACATTCTAGAGTCAGTGAAGCACCCCTTTATTGTGGAACTGGCCTATGCCTTCCAGACT
GGTGGCAAACTCTACCTCATCCTTGAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTG
GAGCGAGAGGGCATCTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTG
GCCCTGGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAGAACATC
ATGCTCAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACTCTGCAAGGAGTCTATC
CATGAGGGCGCCGTCACTCACACCTTCTGCGGCACCATTGAGTACATGGCCCCTGAGATT
CTGGTGCGCAGTGGCCACAACCGGGCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTAC
GACATGCTCACTGGATCGCCGCCCTTCACCGCAGAGAACCGGAAGAAAACCATGGATAAG
ATCATCAGGGGCAAGCTGGCACTGCCCCCCTACCTCACCCCAGATGCCCGGGACCTTGTC
AAAAAGTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGGCCCAGGGGATGCTGCT
GATGTGCAGAGACATCCCTTTTTCCGGCACATGAATTGGGACGACCTTCTGGCCTGGCGT
GTGGACCCCCCTTTCAGGCCCTGTCTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACC
CGCTTCACACGGCAGACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCC
AACCAGGCCTTCCTGGGCTTCACATACGTGGCGCCGTCTGTCCTGGACAGCATCAAGGAG
GGCTTCTCCTTCCAGCCCAAGCTGCGCTCACCCAGGCGCCTCAACAGTAGCCCCCGGGCC
CCCGTCAGCCCCCTCAAGTTCTCCCCTTTTGAGGGGTTTCGGCCCAGCCCCAGCCTGCCG
GAGCCCACGGAGCTACCTCTACCTCCACTCCTGCCACCGCCGCCGCCCTCGACCACCGCC
CCTCTCCCCATCCGTCCCCCCTCAGGGACCAAGAAGTCCAAGAGGGGCCGTGGGCGTCCA
GGGCGC

Protein sequence predicted based on nucleotide sequence

MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPE
RIGPHSFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAER
NILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITL
ALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAVTHTFCGTIEYMAPEI
LVRSGHNRAVDWWSLGALMYDMLTGSPPFTAENRKKTMDKIIRGKLALPPYLTPDARDLV
KKFLKRNPSQRIGGGPGDAADVQRHPFFRHMNWDDLLAWRVDPPFRPCLQSEEDVSQFDT
RFTRQTPVDSPDDTALSESANQAFLGFTYVAPSVLDSIKEGFSFQPKLRSPRRLNSSPRA
PVSPLKFSPFEGFRPSPSLPEPTELPLPPLLPPPPPSTTAPLPIRPPSGTKKSKRGRGRP
GR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RPS6KB2

Symbol Nomenclature :

RPS6KB2

SYNONYM :

KLS; P70-beta; P70-beta-1; P70-beta-2; S6K-beta2; S6K2; SRK; STK14B; p70(S6K)-beta; p70S6Kb

Designation :

Full Nomenclature :

ribosomal protein S6 kinase, 70kDa, polypeptide 2

GENEID :

6199

HGNC :

10437

MIM :

608939

Vega :

OTTHUMG00000167673

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516119      ATGGCGGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAGCGAGGGCGAGGGCGAG
NM_003952.2       ATGGCGGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAGCGAGGGCGAGGGCGAG
                  ************************************************************

HsCD00516119      CCAGAGCTCAGCCCCGCGGACGCATGTCCCCTTGCCGAGTTGAGGGCAGCTGGCCTAGAG
NM_003952.2       CCAGAGCTCAGCCCCGCGGACGCATGTCCCCTTGCCGAGTTGAGGGCAGCTGGCCTAGAG
                  ************************************************************

HsCD00516119      CCTGTGGGACACTATGAAGAGGTGGAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAG
NM_003952.2       CCTGTGGGACACTATGAAGAGGTGGAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAG
                  ************************************************************

HsCD00516119      CGCATCGGGCCCCACTCCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAG
NM_003952.2       CGCATCGGGCCCCACTGCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAG
                  **************** *******************************************

HsCD00516119      GTGTTCCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATGAAAGTC
NM_003952.2       GTGTTCCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATGAAAGTC
                  ************************************************************

HsCD00516119      CTAAGGAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACACACACGGGCTGAGCGG
NM_003952.2       CTAAGGAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACACACACGGGCTGAGCGG
                  ************************************************************

HsCD00516119      AACATTCTAGAGTCAGTGAAGCACCCCTTTATTGTGGAACTGGCCTATGCCTTCCAGACT
NM_003952.2       AACATTCTAGAGTCAGTGAAGCACCCCTTTATTGTGGAACTGGCCTATGCCTTCCAGACT
                  ************************************************************

HsCD00516119      GGTGGCAAACTCTACCTCATCCTTGAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTG
NM_003952.2       GGTGGCAAACTCTACCTCATCCTTGAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTG
                  ************************************************************

HsCD00516119      GAGCGAGAGGGCATCTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTG
NM_003952.2       GAGCGAGAGGGCATCTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTG
                  ************************************************************

HsCD00516119      GCCCTGGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAGAACATC
NM_003952.2       GCCCTGGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAGAACATC
                  ************************************************************

HsCD00516119      ATGCTCAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACTCTGCAAGGAGTCTATC
NM_003952.2       ATGCTCAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACTCTGCAAGGAGTCTATC
                  ************************************************************

HsCD00516119      CATGAGGGCGCCGTCACTCACACCTTCTGCGGCACCATTGAGTACATGGCCCCTGAGATT
NM_003952.2       CATGAGGGCGCCGTCACTCACACCTTCTGCGGCACCATTGAGTACATGGCCCCTGAGATT
                  ************************************************************

HsCD00516119      CTGGTGCGCAGTGGCCACAACCGGGCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTAC
NM_003952.2       CTGGTGCGCAGTGGCCACAACCGGGCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTAC
                  ************************************************************

HsCD00516119      GACATGCTCACTGGATCGCCGCCCTTCACCGCAGAGAACCGGAAGAAAACCATGGATAAG
NM_003952.2       GACATGCTCACTGGATCGCCGCCCTTCACCGCAGAGAACCGGAAGAAAACCATGGATAAG
                  ************************************************************

HsCD00516119      ATCATCAGGGGCAAGCTGGCACTGCCCCCCTACCTCACCCCAGATGCCCGGGACCTTGTC
NM_003952.2       ATCATCAGGGGCAAGCTGGCACTGCCCCCCTACCTCACCCCAGATGCCCGGGACCTTGTC
                  ************************************************************

HsCD00516119      AAAAAGTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGGCCCAGGGGATGCTGCT
NM_003952.2       AAAAAGTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGGCCCAGGGGATGCTGCT
                  ************************************************************

HsCD00516119      GATGTGCAGAGACATCCCTTTTTCCGGCACATGAATTGGGACGACCTTCTGGCCTGGCGT
NM_003952.2       GATGTGCAGAGACATCCCTTTTTCCGGCACATGAATTGGGACGACCTTCTGGCCTGGCGT
                  ************************************************************

HsCD00516119      GTGGACCCCCCTTTCAGGCCCTGTCTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACC
NM_003952.2       GTGGACCCCCCTTTCAGGCCCTGTCTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACC
                  ************************************************************

HsCD00516119      CGCTTCACACGGCAGACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCC
NM_003952.2       CGCTTCACACGGCAGACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCC
                  ************************************************************

HsCD00516119      AACCAGGCCTTCCTGGGCTTCACATACGTGGCGCCGTCTGTCCTGGACAGCATCAAGGAG
NM_003952.2       AACCAGGCCTTCCTGGGCTTCACATACGTGGCGCCGTCTGTCCTGGACAGCATCAAGGAG
                  ************************************************************

HsCD00516119      GGCTTCTCCTTCCAGCCCAAGCTGCGCTCACCCAGGCGCCTCAACAGTAGCCCCCGGGCC
NM_003952.2       GGCTTCTCCTTCCAGCCCAAGCTGCGCTCACCCAGGCGCCTCAACAGTAGCCCCCGGGCC
                  ************************************************************

HsCD00516119      CCCGTCAGCCCCCTCAAGTTCTCCCCTTTTGAGGGGTTTCGGCCCAGCCCCAGCCTGCCG
NM_003952.2       CCCGTCAGCCCCCTCAAGTTCTCCCCTTTTGAGGGGTTTCGGCCCAGCCCCAGCCTGCCG
                  ************************************************************

HsCD00516119      GAGCCCACGGAGCTACCTCTACCTCCACTCCTGCCACCGCCGCCGCCCTCGACCACCGCC
NM_003952.2       GAGCCCACGGAGCTACCTCTACCTCCACTCCTGCCACCGCCGCCGCCCTCGACCACCGCC
                  ************************************************************

HsCD00516119      CCTCTCCCCATCCGTCCCCCCTCAGGGACCAAGAAGTCCAAGAGGGGCCGTGGGCGTCCA
NM_003952.2       CCTCTCCCCATCCGTCCCCCCTCAGGGACCAAGAAGTCCAAGAGGGGCCGTGGGCGTCCA
                  ************************************************************

HsCD00516119      GGGCGC---
NM_003952.2       GGGCGCTAG
                  ******

Pathways

Panther :

Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade

Panther :

p53 pathway by glucose deprivation

Panther :

PDGF signaling pathway

Panther :

PI3 kinase pathway

Reactome :

Adaptive Immune System

Reactome :

AKT phosphorylates targets in the nucleus

Reactome :

Constitutive PI3K/AKT Signaling in Cancer

Reactome :

DAP12 interactions

Reactome :

DAP12 signaling

Reactome :

Disease

Reactome :

Downstream signal transduction

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

Downstream signaling of activated FGFR

Reactome :

Fc epsilon receptor (FCERI) signaling

Reactome :

GAB1 signalosome

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

PI-3K cascade

Reactome :

PI3K events in ERBB2 signaling

Reactome :

PI3K events in ERBB4 signaling

Reactome :

PI3K/AKT activation

Reactome :

PI3K/AKT Signaling in Cancer

Reactome :

PIP3 activates AKT signaling

Reactome :

Role of LAT2/NTAL/LAB on calcium mobilization

Reactome :

Signal Transduction

Reactome :

Signaling by EGFR

Reactome :

Signaling by EGFR in Cancer

Reactome :

Signaling by ERBB2

Reactome :

Signaling by ERBB4

Reactome :

Signaling by FGFR

Reactome :

Signaling by FGFR in disease

Reactome :

Signaling by PDGF

Reactome :

Signaling by SCF-KIT

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signalling by NGF

WikiPathway :

AMPK signaling

WikiPathway :

Cytoplasmic Ribosomal Proteins

WikiPathway :

FSH signaling pathway

WikiPathway :

IL-2 Signaling pathway

WikiPathway :

IL-5 signaling pathway

WikiPathway :

Insulin Signaling

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

SMART domain :

S_TK_X : Extension to Ser/Thr-type protein kinases

HPRD :

10203

Clone

Cloning Information :

669317

Original Clone ID :

ccsbBroadEn_06897

Organism

Species Specific ID:

6199

Genome

Chromosome :

Map Location :

11q13.2

Ensembl :

ENSG00000175634

Reagents

Labome :

RPS6KB2-antibody