DNASU Plasmid | KAL1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KAL1

Gene Name: Kallmann syndrome 1 sequence

Original Clone ID:

ccsbBroadEn_06467

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC137426.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516163

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2040nts

Open reading frame: 1 to 2040

Reference Sequence Alignment

ATGGTGCCCGGGGTGCCCGGCGCGGTCCTGACCCTCTGCCTCTGGCTGGCGGCCTCCAGC
GGCTGCCTGGCGGCCGGCCCCGGCGCGGCTGCTGCGCGGCGGCTGGACGAGTCGCTGTCT
GCCGGGAGCGTCCAGCGCGCTCGCTGCGCCTCCAGGTGCCTGAGCCTGCAGATCACTCGC
ATCTCCGCCTTCTTCCAGCACTTCCAGAACAATGGTTCCCTGGTTTGGTGCCAGAATCAC
AAGCAATGTTCTAAGTGCCTGGAGCCCTGCAAGGAATCAGGGGACCTGAGGAAACACCAG
TGCCAAAGCTTTTGTGAGCCTCTCTTCCCCAAGAAGAGCTACGAATGCTTGACCAGCTGT
GAGTTCCTCAAATACATCCTGTTGGTGAAGCAGGGGGACTGTCCGGCTCCTGAGAAAGCC
AGTGGATTTGCGGCCGCCTGTGTTGAAAGCTGCGAAGTTGACAATGAGTGCTCTGGGGTG
AAGAAATGTTGTTCGAATGGGTGTGGACACACCTGTCAAGTACCCAAGACTCTGTACAAA
GGTGTCCCCCTGAAGCCCAGAAAAGAGTTACGATTTACAGAACTGCAGTCTGGACAGCTG
GAGGTTAAGTGGTCCTCGAAATTCAATATTTCTATTGAGCCTGTGATCTATGTGGTACAA
AGAAGATGGAATTATGGAATCCATCCTAGCGAAGATGACGCCACTCACTGGCAGACAGTG
GCCCAGACCACAGACGAGCGAGTTCAACTGACTGACATAAGACCCAGCCGATGGTACCAG
TTTCGAGTGGCTGCTGTGAATGTGCATGGAACTCGAGGCTTCACTGCCCCCAGCAAACAC
TTCCGTTCTTCCAAAGATCCATCTGCCCCACCAGCACCGGCTAACCTCCGGCTGGCCAAC
TCCACCGTCAACAGTGATGGGAGTGTGACCGTCACTATAGTTTGGGATCTCCCCGAGGAG
CCGGACATCCCTGTGCATCATTACAAGGTCTTTTGGAGCTGGATGGTCAGCAGTAAGTCT
CTTGTCCCAACAAAGAAGAAGCGGAGAAAGACTACGGATGGGTTTCAAAATTCTGTGATC
CTGGAGAAACTCCAGCCAGACTGTGACTATGTTGTGGAATTGCAAGCCATAACGTACTGG
GGACAGACACGGCTGAAGAGTGCAAAGGTGTCCCTTCACTTCACATCGACACATGCAACC
AACAACAAAGAACAGCTTGTGAAAACTAGAAAAGGTGGAATTCAAACACAACTCCCTTTT
CAAAGACGACGACCCACTCGCCCGCTGGAAGTCGGAGCTCCCTTCTATCAGGATGGCCAA
CTGCAAGTTAAAGTCTACTGGAAGAAGACAGAAGATCCCACTGTCAACCGATATCATGTG
CGGTGGTTTCCTGAAGCGTGTGCCCACAACAGAACAACCGGATCAGAGGCATCATCTGGC
ATGACCCACGAAAATTACATAATTCTTCAAGATCTGTCATTTTCCTGCAAGTATAAGGTG
ACTGTCCAACCAATACGGCCAAAAAGTCACTCCAAGGCAGAAGCTGTTTTCTTCACTACT
CCACCATGCTCTGCTCTTAAGGGGAAGAGCCACAAGCCTATTGGCTGCCTGGGCGAAGCA
GGTCATGTTCTTTCTAAGGTGCTAGCTAAGCCTGAGAACCTTTCTGCTTCATTCATCGTC
CAGGATGTGAACATCACCGGTCACTTTTCTTGGAAGATGGCCAAGGCCAATCTCTATCAG
CCCATGACTGGGTTTCAAGTGACTTGGGCTGAGGTCACTACGGAAAGCAGACAGAACAGC
CTACCCAACAGCATTATTTCACAGTCCCAGATTCTGCCTTCCGATCATTATGTCCTAACA
GTGCCCAATCTGAGACCATCTACTCTTTACCGACTGGAAGTGCAAGTGCTGACCCCAGGA
GGGGAGGGGCCGGCCACCATCAAGACGTTCCGGACGCCGGAGCTCCCACCCTCTTCAGCA
CACAGATCTCATCTTAAGCATCGTCATCCACATCATTACAAGCCTTCTCCAGAAAGATAC

Protein sequence predicted based on nucleotide sequence

MVPGVPGAVLTLCLWLAASSGCLAAGPGAAAARRLDESLSAGSVQRARCASRCLSLQITR
ISAFFQHFQNNGSLVWCQNHKQCSKCLEPCKESGDLRKHQCQSFCEPLFPKKSYECLTSC
EFLKYILLVKQGDCPAPEKASGFAAACVESCEVDNECSGVKKCCSNGCGHTCQVPKTLYK
GVPLKPRKELRFTELQSGQLEVKWSSKFNISIEPVIYVVQRRWNYGIHPSEDDATHWQTV
AQTTDERVQLTDIRPSRWYQFRVAAVNVHGTRGFTAPSKHFRSSKDPSAPPAPANLRLAN
STVNSDGSVTVTIVWDLPEEPDIPVHHYKVFWSWMVSSKSLVPTKKKRRKTTDGFQNSVI
LEKLQPDCDYVVELQAITYWGQTRLKSAKVSLHFTSTHATNNKEQLVKTRKGGIQTQLPF
QRRRPTRPLEVGAPFYQDGQLQVKVYWKKTEDPTVNRYHVRWFPEACAHNRTTGSEASSG
MTHENYIILQDLSFSCKYKVTVQPIRPKSHSKAEAVFFTTPPCSALKGKSHKPIGCLGEA
GHVLSKVLAKPENLSASFIVQDVNITGHFSWKMAKANLYQPMTGFQVTWAEVTTESRQNS
LPNSIISQSQILPSDHYVLTVPNLRPSTLYRLEVQVLTPGGEGPATIKTFRTPELPPSSA
HRSHLKHRHPHHYKPSPERY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KAL1

Symbol Nomenclature :

KAL1

SYNONYM :

ADMLX; HH1; HHA; KAL; KALIG-1; KMS; WFDC19

Designation :

Full Nomenclature :

Kallmann syndrome 1 sequence

GENEID :

3730

HGNC :

6211

MIM :

300836

Vega :

OTTHUMG00000021107

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516163      ATGGTGCCCGGGGTGCCCGGCGCGGTCCTGACCCTCTGCCTCTGGCTGGCGGCCTCCAGC
NM_000216.2       ATGGTGCCCGGGGTGCCCGGCGCGGTCCTGACCCTCTGCCTCTGGCTGGCGGCCTCCAGC
                  ************************************************************

HsCD00516163      GGCTGCCTGGCGGCCGGCCCCGGCGCGGCTGCTGCGCGGCGGCTGGACGAGTCGCTGTCT
NM_000216.2       GGCTGCCTGGCGGCCGGCCCCGGCGCGGCTGCTGCGCGGCGGCTGGACGAGTCGCTGTCT
                  ************************************************************

HsCD00516163      GCCGGGAGCGTCCAGCGCGCTCGCTGCGCCTCCAGGTGCCTGAGCCTGCAGATCACTCGC
NM_000216.2       GCCGGGAGCGTCCAGCGCGCTCGCTGCGCCTCCAGGTGCCTGAGCCTGCAGATCACTCGC
                  ************************************************************

HsCD00516163      ATCTCCGCCTTCTTCCAGCACTTCCAGAACAATGGTTCCCTGGTTTGGTGCCAGAATCAC
NM_000216.2       ATCTCCGCCTTCTTCCAGCACTTCCAGAACAATGGTTCCCTGGTTTGGTGCCAGAATCAC
                  ************************************************************

HsCD00516163      AAGCAATGTTCTAAGTGCCTGGAGCCCTGCAAGGAATCAGGGGACCTGAGGAAACACCAG
NM_000216.2       AAGCAATGTTCTAAGTGCCTGGAGCCCTGCAAGGAATCAGGGGACCTGAGGAAACACCAG
                  ************************************************************

HsCD00516163      TGCCAAAGCTTTTGTGAGCCTCTCTTCCCCAAGAAGAGCTACGAATGCTTGACCAGCTGT
NM_000216.2       TGCCAAAGCTTTTGTGAGCCTCTCTTCCCCAAGAAGAGCTACGAATGCTTGACCAGCTGT
                  ************************************************************

HsCD00516163      GAGTTCCTCAAATACATCCTGTTGGTGAAGCAGGGGGACTGTCCGGCTCCTGAGAAAGCC
NM_000216.2       GAGTTCCTCAAATACATCCTGTTGGTGAAGCAGGGGGACTGTCCGGCTCCTGAGAAAGCC
                  ************************************************************

HsCD00516163      AGTGGATTTGCGGCCGCCTGTGTTGAAAGCTGCGAAGTTGACAATGAGTGCTCTGGGGTG
NM_000216.2       AGTGGATTTGCGGCCGCCTGTGTTGAAAGCTGCGAAGTTGACAATGAGTGCTCTGGGGTG
                  ************************************************************

HsCD00516163      AAGAAATGTTGTTCGAATGGGTGTGGACACACCTGTCAAGTACCCAAGACTCTGTACAAA
NM_000216.2       AAGAAATGTTGTTCGAATGGGTGTGGACACACCTGTCAAGTACCCAAGACTCTGTACAAA
                  ************************************************************

HsCD00516163      GGTGTCCCCCTGAAGCCCAGAAAAGAGTTACGATTTACAGAACTGCAGTCTGGACAGCTG
NM_000216.2       GGTGTCCCCCTGAAGCCCAGAAAAGAGTTACGATTTACAGAACTGCAGTCTGGACAGCTG
                  ************************************************************

HsCD00516163      GAGGTTAAGTGGTCCTCGAAATTCAATATTTCTATTGAGCCTGTGATCTATGTGGTACAA
NM_000216.2       GAGGTTAAGTGGTCCTCGAAATTCAATATTTCTATTGAGCCTGTGATCTATGTGGTACAA
                  ************************************************************

HsCD00516163      AGAAGATGGAATTATGGAATCCATCCTAGCGAAGATGACGCCACTCACTGGCAGACAGTG
NM_000216.2       AGAAGATGGAATTATGGAATCCATCCTAGCGAAGATGACGCCACTCACTGGCAGACAGTG
                  ************************************************************

HsCD00516163      GCCCAGACCACAGACGAGCGAGTTCAACTGACTGACATAAGACCCAGCCGATGGTACCAG
NM_000216.2       GCCCAGACCACAGACGAGCGAGTTCAACTGACTGACATAAGACCCAGCCGATGGTACCAG
                  ************************************************************

HsCD00516163      TTTCGAGTGGCTGCTGTGAATGTGCATGGAACTCGAGGCTTCACTGCCCCCAGCAAACAC
NM_000216.2       TTTCGAGTGGCTGCTGTGAATGTGCATGGAACTCGAGGCTTCACTGCCCCCAGCAAACAC
                  ************************************************************

HsCD00516163      TTCCGTTCTTCCAAAGATCCATCTGCCCCACCAGCACCGGCTAACCTCCGGCTGGCCAAC
NM_000216.2       TTCCGTTCTTCCAAAGATCCATCTGCCCCACCAGCACCGGCTAACCTCCGGCTGGCCAAC
                  ************************************************************

HsCD00516163      TCCACCGTCAACAGTGATGGGAGTGTGACCGTCACTATAGTTTGGGATCTCCCCGAGGAG
NM_000216.2       TCCACCGTCAACAGTGATGGGAGTGTGACCGTCACTATAGTTTGGGATCTCCCCGAGGAG
                  ************************************************************

HsCD00516163      CCGGACATCCCTGTGCATCATTACAAGGTCTTTTGGAGCTGGATGGTCAGCAGTAAGTCT
NM_000216.2       CCGGACATCCCTGTGCATCATTACAAGGTCTTTTGGAGCTGGATGGTCAGCAGTAAGTCT
                  ************************************************************

HsCD00516163      CTTGTCCCAACAAAGAAGAAGCGGAGAAAGACTACGGATGGGTTTCAAAATTCTGTGATC
NM_000216.2       CTTGTCCCAACAAAGAAGAAGCGGAGAAAGACTACGGATGGGTTTCAAAATTCTGTGATC
                  ************************************************************

HsCD00516163      CTGGAGAAACTCCAGCCAGACTGTGACTATGTTGTGGAATTGCAAGCCATAACGTACTGG
NM_000216.2       CTGGAGAAACTCCAGCCAGACTGTGACTATGTTGTGGAATTGCAAGCCATAACGTACTGG
                  ************************************************************

HsCD00516163      GGACAGACACGGCTGAAGAGTGCAAAGGTGTCCCTTCACTTCACATCGACACATGCAACC
NM_000216.2       GGACAGACACGGCTGAAGAGTGCAAAGGTGTCCCTTCACTTCACATCGACACATGCAACC
                  ************************************************************

HsCD00516163      AACAACAAAGAACAGCTTGTGAAAACTAGAAAAGGTGGAATTCAAACACAACTCCCTTTT
NM_000216.2       AACAACAAAGAACAGCTTGTGAAAACTAGAAAAGGTGGAATTCAAACACAACTCCCTTTT
                  ************************************************************

HsCD00516163      CAAAGACGACGACCCACTCGCCCGCTGGAAGTCGGAGCTCCCTTCTATCAGGATGGCCAA
NM_000216.2       CAAAGACGACGACCCACTCGCCCGCTGGAAGTCGGAGCTCCCTTCTATCAGGATGGCCAA
                  ************************************************************

HsCD00516163      CTGCAAGTTAAAGTCTACTGGAAGAAGACAGAAGATCCCACTGTCAACCGATATCATGTG
NM_000216.2       CTGCAAGTTAAAGTCTACTGGAAGAAGACAGAAGATCCCACTGTCAACCGATATCATGTG
                  ************************************************************

HsCD00516163      CGGTGGTTTCCTGAAGCGTGTGCCCACAACAGAACAACCGGATCAGAGGCATCATCTGGC
NM_000216.2       CGGTGGTTTCCTGAAGCGTGTGCCCACAACAGAACAACCGGATCAGAGGCATCATCTGGC
                  ************************************************************

HsCD00516163      ATGACCCACGAAAATTACATAATTCTTCAAGATCTGTCATTTTCCTGCAAGTATAAGGTG
NM_000216.2       ATGACCCACGAAAATTACATAATTCTTCAAGATCTGTCATTTTCCTGCAAGTATAAGGTG
                  ************************************************************

HsCD00516163      ACTGTCCAACCAATACGGCCAAAAAGTCACTCCAAGGCAGAAGCTGTTTTCTTCACTACT
NM_000216.2       ACTGTCCAACCAATACGGCCAAAAAGTCACTCCAAGGCAGAAGCTGTTTTCTTCACTACT
                  ************************************************************

HsCD00516163      CCACCATGCTCTGCTCTTAAGGGGAAGAGCCACAAGCCTATTGGCTGCCTGGGCGAAGCA
NM_000216.2       CCACCATGCTCTGCTCTTAAGGGGAAGAGCCACAAGCCTGTTGGCTGCCTGGGCGAAGCA
                  ***************************************.********************

HsCD00516163      GGTCATGTTCTTTCTAAGGTGCTAGCTAAGCCTGAGAACCTTTCTGCTTCATTCATCGTC
NM_000216.2       GGTCATGTTCTTTCTAAGGTGCTAGCTAAGCCTGAGAACCTTTCTGCTTCATTCATCGTC
                  ************************************************************

HsCD00516163      CAGGATGTGAACATCACCGGTCACTTTTCTTGGAAGATGGCCAAGGCCAATCTCTATCAG
NM_000216.2       CAGGATGTGAACATCACCGGTCACTTTTCTTGGAAGATGGCCAAGGCCAATCTCTATCAG
                  ************************************************************

HsCD00516163      CCCATGACTGGGTTTCAAGTGACTTGGGCTGAGGTCACTACGGAAAGCAGACAGAACAGC
NM_000216.2       CCCATGACTGGGTTTCAAGTGACTTGGGCTGAGGTCACTACGGAAAGCAGACAGAACAGC
                  ************************************************************

HsCD00516163      CTACCCAACAGCATTATTTCACAGTCCCAGATTCTGCCTTCCGATCATTATGTCCTAACA
NM_000216.2       CTACCCAACAGCATTATTTCACAGTCCCAGATCCTGCCTTCCGATCATTATGTCCTAACA
                  ******************************** ***************************

HsCD00516163      GTGCCCAATCTGAGACCATCTACTCTTTACCGACTGGAAGTGCAAGTGCTGACCCCAGGA
NM_000216.2       GTGCCCAATCTGAGACCATCTACTCTTTACCGACTGGAAGTGCAAGTGCTGACCCCAGGA
                  ************************************************************

HsCD00516163      GGGGAGGGGCCGGCCACCATCAAGACGTTCCGGACGCCGGAGCTCCCACCCTCTTCAGCA
NM_000216.2       GGGGAGGGGCCGGCCACCATCAAGACGTTCCGGACGCCGGAGCTCCCACCCTCTTCAGCA
                  ************************************************************

HsCD00516163      CACAGATCTCATCTTAAGCATCGTCATCCACATCATTACAAGCCTTCTCCAGAAAGATAC
NM_000216.2       CACAGATCTCATCTTAAGCATCGTCATCCACATCATTACAAGCCTTCTCCAGAAAGATAC
                  ************************************************************

HsCD00516163      ---
NM_000216.2       TAA