DNASU Plasmid | SF4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SUGP1

Gene Name: SURP and G patch domain containing 1

Original Clone ID:

ccsbBroadEn_08767

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC142988.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516189

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1936nts

Open reading frame: 1 to 1936

Reference Sequence Alignment

ATGAGTCTCAAGATGGACAACCGGGATGTTGCAGGAAAGGCTAACCGGTGGTTTGGGGTT
GCTCCCCCTAAATCTGGAAAAATGAACATGAACATCCTTCACCAGGAAGAGCTCATCGCT
CAGAAGAAACGGGAAATTGAAGCCAAAATGGAACAGAAAGCCAAGCAGAATCAGGTGGCC
AGCCCTCAGCCCCCACATCCTGGCGAAATCACAAATGCACACAACTCTTCCTGCATTTCC
AACAAGTTTGCCAACGATGGTAGCTTCTTGCAGCAGTTTCTGAAGTTGCAGAAGGCACAG
ACCAGCACAGACGCCCCGACCAGTGCGCCCAGCGCCCCTCCCAGCACACCCACCCGCAGC
GCTGGGAAGAGGTCCCTGCTCATCAGCAGGCGGACAGGCCTGGGGCTGGCCAGCCTGCCG
GGCCCTGTGAAGAGCTACTCCCACGCCAAGCAGCTGCCCGTGGCGCACCGCCCGAGTGTC
TTCCAGTCCCCTGACGAGGACGAGGAGGAGGACTATGAGCAGTGGCTGGAGATCAAAGTT
TCACCCCCAGAGGGAGCCGAGACTCGGAAAGTGATAGAGAAATTGGCCCGCTTTGTGGCA
GAAGGAGGCCCCGAGTTAGAAAAAGTAGCTATGGAGGACTACAAGGATAACCCAGCATTT
GCATTTTTGCACGATAAGAATAGCAGGGAATTCCTCTACTACAGGAAGAAGGTGGCTGAG
ATAAGAAAGGAAGCACAGAAGTCGCAGGCAGCCTCTCAGAAAGTTTCACCCCCAGAGGAC
GAAGAGGTCAAGAACCTTGCAGAAAAGTTGGCCAGGTTCATAGCGGACGGGGGTCCCGAG
GTGGAAACCATTGCCCTCCAGAACAACCGTGAGAACCAGGCATTCAGCTTTCTGTATGAG
CCCAATAGCCAAGGGTACAAGTACTACCGACAGAAGCTGGAGGAGTTCCGGAAAGCCAAG
GCCAGCTCCACAGGCAGCTTCACAGCACCTGATCCCGGCCTGAAGCGCAAGTCCCCTCCT
GAGGCCCTGTCAGGGTCCTTACCCCCAGCCACCACCTGCCCCGCCTCGTCCACGCCTGCG
CCCACTATCATCCCTGCTCCAGCTGCCCCCGGGAAGCCAGCCTCCGCAGCCACCGTGAAG
AGGAAGCGGAAGAGCCGGTGGGGGCCTGAAGAGGATAAGGTAGAGCTCCCACCTGCTGAA
CTGGTGCAGAGGGACGTGGATGCCTCTCCCTCGCCTCTGTCAGTTCAGGACCTCAAGGGG
CTCGGCTATGAGAAGGGGAAGCCTGTGGGTCTAGTGGGCGTCACAGAGCTTTCAGACGCC
CAGAAGAAGCAGCTGAAGGAGCAGCAGGAGATGCAGCAGATGTACGACATGATCATGCAG
CACAAGCGGGCCATGCAGGACATGCAGCTGCTGTGGGAGAAGGCAGTCCAACAGCACCAG
CACGGCTATGACAGTGATGAGGAGGTGGACAGCGAGCTGGGCACCTGGGAGCACCAGCTG
CGGCGCATGGAGATGGATAAGACCAGGGAATGGGCCGAGCAGCTGACAAAGATGGGCCGG
GGCAAGCACTTCATCGGAGACTTCCTGCCTCCAGACGAGCTGGAAAAGTTTATGGAGACC
TTCAAGGCCCTGAAGGAGGGCCGTGAGCCTGACTACTCAGAGTACAAGGAGTTCAAGCTG
ACTGTGGAGAACATCGGCTACCAGATGCTGATGAAGATGGGCTGGAAGGAGGGCGAGGGG
CTGGGCTCAGAGGGCCAGGGCATCAAGAACCCAGTGAACAAGGGCACCACCACAGTGGAC
GGCGCTGGCTTCGGCATTGACCGGCCGGCGGAGCTCTCCAAGGAGGACGACGAGTATGAG
GCGTTCCGCAAGAGGATGATGCTGGCCTACCGCTTCCGGCCCAACCCCCTGAACAATCCC
AGACGGCCTTACTAC

Protein sequence predicted based on nucleotide sequence

MSLKMDNRDVAGKANRWFGVAPPKSGKMNMNILHQEELIAQKKREIEAKMEQKAKQNQVA
SPQPPHPGEITNAHNSSCISNKFANDGSFLQQFLKLQKAQTSTDAPTSAPSAPPSTPTRS
AGKRSLLISRRTGLGLASLPGPVKSYSHAKQLPVAHRPSVFQSPDEDEEEDYEQWLEIKV
SPPEGAETRKVIEKLARFVAEGGPELEKVAMEDYKDNPAFAFLHDKNSREFLYYRKKVAE
IRKEAQKSQAASQKVSPPEDEEVKNLAEKLARFIADGGPEVETIALQNNRENQAFSFLYE
PNSQGYKYYRQKLEEFRKAKASSTGSFTAPDPGLKRKSPPEALSGSLPPATTCPASSTPA
PTIIPAPAAPGKPASAATVKRKRKSRWGPEEDKVELPPAELVQRDVDASPSPLSVQDLKG
LGYEKGKPVGLVGVTELSDAQKKQLKEQQEMQQMYDMIMQHKRAMQDMQLLWEKAVQQHQ
HGYDSDEEVDSELGTWEHQLRRMEMDKTREWAEQLTKMGRGKHFIGDFLPPDELEKFMET
FKALKEGREPDYSEYKEFKLTVENIGYQMLMKMGWKEGEGLGSEGQGIKNPVNKGTTTVD
GAGFGIDRPAELSKEDDEYEAFRKRMMLAYRFRPNPLNNPRRPYY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SUGP1

Symbol Nomenclature :

SUGP1

SYNONYM :

F23858; RBP; SF4

Designation :

RNA-binding protein RBP|SURP and G-patch domain-containing protein 1|splicing factor 4

Full Nomenclature :

SURP and G patch domain containing 1

GENEID :

57794

HGNC :

18643

MIM :

607992

Vega :

OTTHUMG00000182223

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516189      ATGAGTCTCAAGATGGACAACCGGGATGTTGCAGGAAAGGCTAACCGGTGGTTTGGGGTT
NM_172231.3       ATGAGTCTCAAGATGGACAACCGGGATGTTGCAGGAAAGGCTAACCGGTGGTTTGGGGTT
                  ************************************************************

HsCD00516189      GCTCCCCCTAAATCTGGAAAAATGAACATGAACATCCTTCACCAGGAAGAGCTCATCGCT
NM_172231.3       GCTCCCCCTAAATCTGGAAAAATGAACATGAACATCCTTCACCAGGAAGAGCTCATCGCT
                  ************************************************************

HsCD00516189      CAGAAGAAACGGGAAATTGAAGCCAAAATGGAACAGAAAGCCAAGCAGAATCAGGTGGCC
NM_172231.3       CAGAAGAAACGGGAAATTGAAGCCAAAATGGAACAGAAAGCCAAGCAGAATCAGGTGGCC
                  ************************************************************

HsCD00516189      AGCCCTCAGCCCCCACATCCTGGCGAAATCACAAATGCACACAACTCTTCCTGCATTTCC
NM_172231.3       AGCCCTCAGCCCCCACATCCTGGCGAAATCACAAATGCACACAACTCTTCCTGCATTTCC
                  ************************************************************

HsCD00516189      AACAAGTTTGCCAACGATGGTAGCTTCTTGCAGCAGTTTCTGAAGTTGCAGAAGGCACAG
NM_172231.3       AACAAGTTTGCCAACGATGGTAGCTTCTTGCAGCAGTTTCTGAAGTTGCAGAAGGCACAG
                  ************************************************************

HsCD00516189      ACCAGCACAGACGCCCCGACCAGTGCGCCCAGCGCCCCTCCCAGCACACCCACCCGCAGC
NM_172231.3       ACCAGCACAGACGCCCCGACCAGTGCGCCCAGCGCCCCTCCCAGCACACCCACCCCCAGC
                  ******************************************************* ****

HsCD00516189      GCTGGGAAGAGGTCCCTGCTCATCAGCAGGCGGACAGGCCTGGGGCTGGCCAGCCTGCCG
NM_172231.3       GCTGGGAAGAGGTCCCTGCTCATCAGCAGGCGGACAGGCCTGGGGCTGGCCAGCCTGCCG
                  ************************************************************

HsCD00516189      GGCCCTGTGAAGAGCTACTCCCACGCCAAGCAGCTGCCCGTGGCGCACCGCCCGAGTGTC
NM_172231.3       GGCCCTGTGAAGAGCTACTCCCACGCCAAGCAGCTGCCCGTGGCGCACCGCCCGAGTGTC
                  ************************************************************

HsCD00516189      TTCCAGTCCCCTGACGAGGACGAGGAGGAGGACTATGAGCAGTGGCTGGAGATCAAAGTT
NM_172231.3       TTCCAGTCCCCTGACGAGGACGAGGAGGAGGACTATGAGCAGTGGCTGGAGATCAAAGTT
                  ************************************************************

HsCD00516189      TCACCCCCAGAGGGAGCCGAGACTCGGAAAGTGATAGAGAAATTGGCCCGCTTTGTGGCA
NM_172231.3       TCACCCCCAGAGGGAGCCGAGACTCGGAAAGTGATAGAGAAATTGGCCCGCTTTGTGGCA
                  ************************************************************

HsCD00516189      GAAGGAGGCCCCGAGTTAGAAAAAGTAGCTATGGAGGACTACAAGGATAACCCAGCATTT
NM_172231.3       GAAGGAGGCCCCGAGTTAGAAAAAGTAGCTATGGAGGACTACAAGGATAACCCAGCATTT
                  ************************************************************

HsCD00516189      GCATTTTTGCACGATAAGAATAGCAGGGAATTCCTCTACTACAGGAAGAAGGTGGCTGAG
NM_172231.3       GCATTTTTGCACGATAAGAATAGCAGGGAATTCCTCTACTACAGGAAGAAGGTGGCTGAG
                  ************************************************************

HsCD00516189      ATAAGAAAGGAAGCACAGAAGTCGCAGGCAGCCTCTCAGAAAGTTTCACCCCCAGAGGAC
NM_172231.3       ATAAGAAAGGAAGCACAGAAGTCGCAGGCAGCCTCTCAGAAAGTTTCACCCCCAGAGGAC
                  ************************************************************

HsCD00516189      GAAGAGGTCAAGAACCTTGCAGAAAAGTTGGCCAGGTTCATAGCGGACGGGGGTCCCGAG
NM_172231.3       GAAGAGGTCAAGAACCTTGCAGAAAAGTTGGCCAGGTTCATAGCGGACGGGGGTCCCGAG
                  ************************************************************

HsCD00516189      GTGGAAACCATTGCCCTCCAGAACAACCGTGAGAACCAGGCATTCAGCTTTCTGTATGAG
NM_172231.3       GTGGAAACCATTGCCCTCCAGAACAACCGTGAGAACCAGGCATTCAGCTTTCTGTATGAG
                  ************************************************************

HsCD00516189      CCCAATAGCCAAGGGTACAAGTACTACCGACAGAAGCTGGAGGAGTTCCGGAAAGCCAAG
NM_172231.3       CCCAATAGCCAAGGGTACAAGTACTACCGACAGAAGCTGGAGGAGTTCCGGAAAGCCAAG
                  ************************************************************

HsCD00516189      GCCAGCTCCACAGGCAGCTTCACAGCACCTGATCCCGGCCTGAAGCGCAAGTCCCCTCCT
NM_172231.3       GCCAGCTCCACAGGCAGCTTCACAGCACCTGATCCCGGCCTGAAGCGCAAGTCCCCTCCT
                  ************************************************************

HsCD00516189      GAGGCCCTGTCAGGGTCCTTACCCCCAGCCACCACCTGCCCCGCCTCGTCCACGCCTGCG
NM_172231.3       GAGGCCCTGTCAGGGTCCTTACCCCCAGCCACCACCTGCCCCGCCTCGTCCACGCCTGCG
                  ************************************************************

HsCD00516189      CCCACTATCATCCCTGCTCCAGCTGCCCCCGGGAAGCCAGCCTCCGCAGCCACCGTGAAG
NM_172231.3       CCCACTATCATCCCTGCTCCAGCTGCCCCCGGGAAGCCAGCCTCCGCAGCCACCGTGAAG
                  ************************************************************

HsCD00516189      AGGAAGCGGAAGAGCCGGTGGGGGCCTGAAGAGGATAAGGTAGAGCTCCCACCTGCTGAA
NM_172231.3       AGGAAGCGGAAGAGCCGGTGGGGGCCTGAAGAGGATAAGGTAGAGCTCCCACCTGCTGAA
                  ************************************************************

HsCD00516189      CTGGTGCAGAGGGACGTGGATGCCTCTCCCTCGCCTCTGTCAGTTCAGGACCTCAAGGGG
NM_172231.3       CTGGTGCAGAGGGACGTGGATGCCTCTCCCTCGCCTCTGTCAGTTCAGGACCTCAAGGGG
                  ************************************************************

HsCD00516189      CTCGGCTATGAGAAGGGGAAGCCTGTGGGTCTAGTGGGCGTCACAGAGCTTTCAGACGCC
NM_172231.3       CTCGGCTATGAGAAGGGGAAGCCTGTGGGTCTAGTGGGCGTCACAGAGCTTTCAGACGCC
                  ************************************************************

HsCD00516189      CAGAAGAAGCAGCTGAAGGAGCAGCAGGAGATGCAGCAGATGTACGACATGATCATGCAG
NM_172231.3       CAGAAGAAGCAGCTGAAGGAGCAGCAGGAGATGCAGCAGATGTACGACATGATCATGCAG
                  ************************************************************

HsCD00516189      CACAAGCGGGCCATGCAGGACATGCAGCTGCTGTGGGAGAAGGCAGTCCAACAGCACCAG
NM_172231.3       CACAAGCGGGCCATGCAGGACATGCAGCTGCTGTGGGAGAAGGCAGTCCAACAGCACCAG
                  ************************************************************

HsCD00516189      CACGGCTATGACAGTGATGAGGAGGTGGACAGCGAGCTGGGCACCTGGGAGCACCAGCTG
NM_172231.3       CACGGCTATGACAGTGATGAGGAGGTGGACAGCGAGCTGGGCACCTGGGAGCACCAGCTG
                  ************************************************************

HsCD00516189      CGGCGCATGGAGATGGATAAGACCAGGGAATGGGCCGAGCAGCTGACAAAGATGGGCCGG
NM_172231.3       CGGCGCATGGAGATGGATAAGACCAGGGAATGGGCCGAGCAGCTGACAAAGATGGGCCGG
                  ************************************************************

HsCD00516189      GGCAAGCACTTCATCGGAGACTTCCTGCCTCCAGACGAGCTGGAAAAGTTTATGGAGACC
NM_172231.3       GGCAAGCACTTCATCGGAGACTTCCTGCCTCCAGACGAGCTGGAAAAGTTTATGGAGACC
                  ************************************************************

HsCD00516189      TTCAAGGCCCTGAAGGAGGGCCGTGAGCCTGACTACTCAGAGTACAAGGAGTTCAAGCTG
NM_172231.3       TTCAAGGCCCTGAAGGAGGGCCGTGAGCCTGACTACTCAGAGTACAAGGAGTTCAAGCTG
                  ************************************************************

HsCD00516189      ACTGTGGAGAACATCGGCTACCAGATGCTGATGAAGATGGGCTGGAAGGAGGGCGAGGGG
NM_172231.3       ACTGTGGAGAACATCGGCTACCAGATGCTGATGAAGATGGGCTGGAAGGAGGGCGAGGGG
                  ************************************************************

HsCD00516189      CTGGGCTCAGAGGGCCAGGGCATCAAGAACCCAGTGAACAAGGGCACCACCACAGTGGAC
NM_172231.3       CTGGGCTCAGAGGGCCAGGGCATCAAGAACCCAGTGAACAAGGGCACCACCACAGTGGAC
                  ************************************************************

HsCD00516189      GGCGCTGGCTTCGGCATTGACCGGCCGGCGGAGCTCTCCAAGGAGGACGACGAGTATGAG
NM_172231.3       GGCGCTGGCTTCGGCATTGACCGGCCGGCGGAGCTCTCCAAGGAGGACGACGAGTATGAG
                  ************************************************************

HsCD00516189      GCGTTCCGCAAGAGGATGATGCTGGCCTACCGCTTCCGGCCCAACCCCCTGAACAATCCC
NM_172231.3       GCGTTCCGCAAGAGGATGATGCTGGCCTACCGCTTCCGGCCCAACCCCCTGAACAATCCC
                  ************************************************************

HsCD00516189      AGACGGCCTTACTAC---
NM_172231.3       AGACGGCCTTACTACTGA
                  ***************