DNASU Plasmid | TBC1D25 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TBC1D25

Gene Name: TBC1 domain family, member 25

Original Clone ID:

ccsbBroadEn_06666

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC125088.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516201

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2064nts

Open reading frame: 1 to 2064

Reference Sequence Alignment

ATGGCAACAGCCTCCGGGGCCTCGGACTTGTCTGGCTCCGGAGCGCCCCCGCCCGGTGTG
GGAGCTCAGGCGGCGGCGGCCGCTGAGGAGGAGGAGCGAGAGGTGGTGCGGGTCCGAGTC
AAGAAATGTGAGAGCTTCTTGCCGCCAGAGTTCCGCTCTTTTGCTGTAGATCCCCAGATC
ACCTCGCTCGACGTGTTGCAGCACATCCTCATCCGAGCCTTTGATTTGAGTGGGAAGAAG
AACTTTGGCATCAGCTACCTGGGCCGGGACCGGCTAGGACAGGAAGTTTACCTCTCACTT
CTATCTGACTGGGACCTCAGCACAGCCTTTGCCACTGCCTCCAAACCTTACCTGCAATTG
CGTGTAGATATTCGGCCCTCGGAGGACAGCCCATTGCTAGAAGACTGGGACATAATCAGC
CCCAAAGATGTCATTGGCTCCGACGTGTTGCTGGCTGAGAAACGGTCATCACTGACGACT
GCCGCCCTGCCCTTTACACAGTCCATCCTCACTCAGGTGGGCCGTACCTTGTCTAAGGTC
CAACAAGTGCTGAGCTGGTCGTATGGGGAAGATGTCAAGCCCTTCAAGCCACCCCTGAGC
GATGCTGAGTTTCACACGTACCTGAACCACGAGGGCCAGCTCTCCCGACCCGAGGAGTTG
CGCCTGCGGATCTATCATGGCGGTGTGGAGCCCTCGCTGCGAAAGGTGGTGTGGCGGTAC
CTGCTGAACGTGTATCCAGATGGACTGACAGGCCGAGAGCGGATGGACTACATGAAACGC
AAGAGCCGCGAGTATGAGCAGCTCAAGAGCGAGTGGGCCCAGCGAGCGAGCCCTGAGGAC
CTGGAATTCATCCGCAGCACGGTCCTCAAGGATGTACTGCGCACTGACCGGGCCCACCCC
TACTATGCGGGGCCTGAGGATGGCCCACATCTACGGGCGCTGCACGACCTGCTCACCACC
TATGCCGTTACCCACCCACAGGTGTCCTACTGCCAGGGCATGAGTGACCTTGCCTCACCC
ATCCTCGCTGTCATGGACCATGAGGGCCATGCCTTTGTTTGCTTTTGTGGCATCATGAAA
CGCCTGGCCGCCAACTTCCACCCTGACGGCCGCGCCATGGCCACCAAGTTTGCACACTTG
AAGCTGTTGCTGCGACACGCTGACCCTGACTTTTATCAATACCTGCAAGAGGCAGGCGCT
GATGACCTCTTCTTCTGTTACCGCTGGCTGCTGCTGGAACTCAAGCGTGAGTTCGCCTTC
GACGATGCCCTCCGCATGCTTGAGGTCACTTGGAGTTCGCTGCCCCCTGATCCTCCTGAA
CATGAGGTAGAGCTGGTTGGACCCCCCAGCCAAGTGGCAGACACTGGTTTTGGTGGCCAC
AGGGGGTGGCCCGTGCGACAGAGGCACATGCTGAGGCCTGCTGGTGGAGGAGGTAGTACC
TTTGAAGATGCTGTTGACCACCTGGCCACAGCCAGTCAGGGGCCTGGTGGTGGGGGGCGT
CTCCTGAGACAGGCCAGCTTGGATGGCCTCCAGCAACTCAGGGATAACATGGGCTCCAGG
AGGGACCCTCTGGTCCAGCTGCCCCACCCAGCTGCCCTTATCAGCTCCAAGTCCCTCTCT
GAGCCTTTATTGAACTCCCCAGACCCACTGCTCTCCTCCTTTTCCCACCCTGATTCCCCA
TCTTCCTCATCTCCACCATCCACCCAGGAGGCCTCTCCCACTGGTGATATGGCTGTAGGA
TCCCCCTTGATGCAAGAGGTAGGCTCCCCGAAAGACCCTGGAAAGTCCCTGCCACCTGTA
CCACCAATGGGCCTGCCCCCACCCCAGGAGTTTGGCCGGGGGAACCCATTCATGCTGTTC
CTCTGCCTGGCCATCCTGCTGGAGCACCGCGACCACATCATGCGCAATGGGCTGGATTAT
AATGAGCTGGCCATGCACTTTGACCGCCTTGTGCGAAAACACCACCTGGGGCGCGTCCTG
CGCCGGGCTAGGGCTCTCTTTGCTGATTACCTGCAGTCAGAGGTGTGGGACTCAGAGGAG
GGGGCTGAGGCCACAGCCGCATCT

Protein sequence predicted based on nucleotide sequence

MATASGASDLSGSGAPPPGVGAQAAAAAEEEEREVVRVRVKKCESFLPPEFRSFAVDPQI
TSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQL
RVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKV
QQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRY
LLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRASPEDLEFIRSTVLKDVLRTDRAHP
YYAGPEDGPHLRALHDLLTTYAVTHPQVSYCQGMSDLASPILAVMDHEGHAFVCFCGIMK
RLAANFHPDGRAMATKFAHLKLLLRHADPDFYQYLQEAGADDLFFCYRWLLLELKREFAF
DDALRMLEVTWSSLPPDPPEHEVELVGPPSQVADTGFGGHRGWPVRQRHMLRPAGGGGST
FEDAVDHLATASQGPGGGGRLLRQASLDGLQQLRDNMGSRRDPLVQLPHPAALISSKSLS
EPLLNSPDPLLSSFSHPDSPSSSSPPSTQEASPTGDMAVGSPLMQEVGSPKDPGKSLPPV
PPMGLPPPQEFGRGNPFMLFLCLAILLEHRDHIMRNGLDYNELAMHFDRLVRKHHLGRVL
RRARALFADYLQSEVWDSEEGAEATAAS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TBC1D25

Symbol Nomenclature :

TBC1D25

SYNONYM :

MG81; OATL1

Designation :

5SN3 snoRNA|TBC1 domain family member 25|ornithine aminotransferase-like 1

Full Nomenclature :

TBC1 domain family, member 25

GENEID :

4943

HGNC :

8092

MIM :

311240

Vega :

OTTHUMG00000024123

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516201      ATGGCAACAGCCTCCGGGGCCTCGGACTTGTCTGGCTCCGGAGCGCCCCCGCCCGGTGTG
NM_002536.2       ATGGCAACAGCCTCCGGGGCCTCGGACTTGTCTGGCTCCGGAGCGCCCCCGCCCGGTGTG
                  ************************************************************

HsCD00516201      GGAGCTCAGGCGGCGGCGGCCGCTGAGGAGGAGGAGCGAGAGGTGGTGCGGGTCCGAGTC
NM_002536.2       GGAGCTCAGGCGGCGGCGGCCGCTGAGGAGGAGGAGCGAGAGGTGGTGCGGGTCCGAGTC
                  ************************************************************

HsCD00516201      AAGAAATGTGAGAGCTTCTTGCCGCCAGAGTTCCGCTCTTTTGCTGTAGATCCCCAGATC
NM_002536.2       AAGAAATGTGAGAGCTTCTTGCCGCCAGAGTTCCGCTCTTTTGCTGTAGATCCCCAGATC
                  ************************************************************

HsCD00516201      ACCTCGCTCGACGTGTTGCAGCACATCCTCATCCGAGCCTTTGATTTGAGTGGGAAGAAG
NM_002536.2       ACCTCGCTCGACGTGTTGCAGCACATCCTCATCCGAGCCTTTGATTTGAGTGGGAAGAAG
                  ************************************************************

HsCD00516201      AACTTTGGCATCAGCTACCTGGGCCGGGACCGGCTAGGACAGGAAGTTTACCTCTCACTT
NM_002536.2       AACTTTGGCATCAGCTACCTGGGCCGGGACCGGCTAGGACAGGAAGTTTACCTCTCACTT
                  ************************************************************

HsCD00516201      CTATCTGACTGGGACCTCAGCACAGCCTTTGCCACTGCCTCCAAACCTTACCTGCAATTG
NM_002536.2       CTATCTGACTGGGACCTCAGCACAGCCTTTGCCACTGCCTCCAAACCTTACCTGCAATTG
                  ************************************************************

HsCD00516201      CGTGTAGATATTCGGCCCTCGGAGGACAGCCCATTGCTAGAAGACTGGGACATAATCAGC
NM_002536.2       CGTGTAGATATTCGGCCCTCGGAGGACAGCCCATTGCTAGAAGACTGGGACATAATCAGC
                  ************************************************************

HsCD00516201      CCCAAAGATGTCATTGGCTCCGACGTGTTGCTGGCTGAGAAACGGTCATCACTGACGACT
NM_002536.2       CCCAAAGATGTCATTGGCTCCGACGTGTTGCTGGCTGAGAAACGGTCATCACTGACGACT
                  ************************************************************

HsCD00516201      GCCGCCCTGCCCTTTACACAGTCCATCCTCACTCAGGTGGGCCGTACCTTGTCTAAGGTC
NM_002536.2       GCCGCCCTGCCCTTTACACAGTCCATCCTCACTCAGGTGGGCCGTACCTTGTCTAAGGTC
                  ************************************************************

HsCD00516201      CAACAAGTGCTGAGCTGGTCGTATGGGGAAGATGTCAAGCCCTTCAAGCCACCCCTGAGC
NM_002536.2       CAACAAGTGCTGAGCTGGTCGTATGGGGAAGATGTCAAGCCCTTCAAGCCACCCCTGAGC
                  ************************************************************

HsCD00516201      GATGCTGAGTTTCACACGTACCTGAACCACGAGGGCCAGCTCTCCCGACCCGAGGAGTTG
NM_002536.2       GATGCTGAGTTTCACACGTACCTGAACCACGAGGGCCAGCTCTCCCGACCCGAGGAGTTG
                  ************************************************************

HsCD00516201      CGCCTGCGGATCTATCATGGCGGTGTGGAGCCCTCGCTGCGAAAGGTGGTGTGGCGGTAC
NM_002536.2       CGCCTGCGGATCTATCATGGCGGTGTGGAGCCCTCGCTGCGAAAGGTGGTGTGGCGGTAC
                  ************************************************************

HsCD00516201      CTGCTGAACGTGTATCCAGATGGACTGACAGGCCGAGAGCGGATGGACTACATGAAACGC
NM_002536.2       CTGCTGAACGTGTATCCAGATGGACTGACAGGCCGAGAGCGGATGGACTACATGAAACGC
                  ************************************************************

HsCD00516201      AAGAGCCGCGAGTATGAGCAGCTCAAGAGCGAGTGGGCCCAGCGAGCGAGCCCTGAGGAC
NM_002536.2       AAGAGCCGCGAGTATGAGCAGCTCAAGAGCGAGTGGGCCCAGCGAGCGAACCCTGAGGAC
                  *************************************************.**********

HsCD00516201      CTGGAATTCATCCGCAGCACGGTCCTCAAGGATGTACTGCGCACTGACCGGGCCCACCCC
NM_002536.2       CTGGAATTCATCCGCAGCACGGTCCTCAAGGATGTACTGCGCACTGACCGGGCCCACCCC
                  ************************************************************

HsCD00516201      TACTATGCGGGGCCTGAGGATGGCCCACATCTACGGGCGCTGCACGACCTGCTCACCACC
NM_002536.2       TACTATGCGGGGCCTGAGGATGGCCCACATCTACGGGCGCTGCACGACCTGCTCACCACC
                  ************************************************************

HsCD00516201      TATGCCGTTACCCACCCACAGGTGTCCTACTGCCAGGGCATGAGTGACCTTGCCTCACCC
NM_002536.2       TATGCCGTTACCCACCCACAGGTGTCCTACTGCCAGGGCATGAGTGACCTTGCCTCACCC
                  ************************************************************

HsCD00516201      ATCCTCGCTGTCATGGACCATGAGGGCCATGCCTTTGTTTGCTTTTGTGGCATCATGAAA
NM_002536.2       ATCCTCGCTGTCATGGACCATGAGGGCCATGCCTTTGTTTGCTTTTGTGGCATCATGAAA
                  ************************************************************

HsCD00516201      CGCCTGGCCGCCAACTTCCACCCTGACGGCCGCGCCATGGCCACCAAGTTTGCACACTTG
NM_002536.2       CGCCTGGCCGCCAACTTCCACCCTGACGGCCGCGCCATGGCCACCAAGTTTGCACACTTG
                  ************************************************************

HsCD00516201      AAGCTGTTGCTGCGACACGCTGACCCTGACTTTTATCAATACCTGCAAGAGGCAGGCGCT
NM_002536.2       AAGCTGTTGCTGCGACACGCTGACCCTGACTTTTATCAATACCTGCAAGAGGCAGGCGCT
                  ************************************************************

HsCD00516201      GATGACCTCTTCTTCTGTTACCGCTGGCTGCTGCTGGAACTCAAGCGTGAGTTCGCCTTC
NM_002536.2       GATGACCTCTTCTTCTGTTACCGCTGGCTGCTGCTGGAACTCAAGCGTGAGTTCGCCTTC
                  ************************************************************

HsCD00516201      GACGATGCCCTCCGCATGCTTGAGGTCACTTGGAGTTCGCTGCCCCCTGATCCTCCTGAA
NM_002536.2       GACGATGCCCTCCGCATGCTTGAGGTCACTTGGAGTTCGCTGCCCCCTGATCCTCCTGAA
                  ************************************************************

HsCD00516201      CATGAGGTAGAGCTGGTTGGACCCCCCAGCCAAGTGGCAGACACTGGTTTTGGTGGCCAC
NM_002536.2       CATGAGGTAGAGCTGGTTGGACCCCCCAGCCAAGTGGCAGACGCTGGTTTTGGTGGCCAC
                  ******************************************.*****************

HsCD00516201      AGGGGGTGGCCCGTGCGACAGAGGCACATGCTGAGGCCTGCTGGTGGAGGAGGTAGTACC
NM_002536.2       AGGGGGTGGCCCGTGCGACAGAGGCACATGCTGAGGCCTGCTGGTGGAGGAGGTAGTACC
                  ************************************************************

HsCD00516201      TTTGAAGATGCTGTTGACCACCTGGCCACAGCCAGTCAGGGGCCTGGTGGTGGGGGGCGT
NM_002536.2       TTTGAAGATGCTGTTGACCACCTGGCCACAGCCAGTCAGGGGCCTGGTGGTGGGGGGCGT
                  ************************************************************

HsCD00516201      CTCCTGAGACAGGCCAGCTTGGATGGCCTCCAGCAACTCAGGGATAACATGGGCTCCAGG
NM_002536.2       CTCCTGAGACAGGCCAGCTTGGATGGCCTCCAGCAACTCAGGGATAACATGGGCTCCAGG
                  ************************************************************

HsCD00516201      AGGGACCCTCTGGTCCAGCTGCCCCACCCAGCTGCCCTTATCAGCTCCAAGTCCCTCTCT
NM_002536.2       AGGGACCCTCTGGTCCAGCTGCCCCACCCAGCTGCCCTTATCAGCTCCAAGTCCCTCTCT
                  ************************************************************

HsCD00516201      GAGCCTTTATTGAACTCCCCAGACCCACTGCTCTCCTCCTTTTCCCACCCTGATTCCCCA
NM_002536.2       GAGCCTTTATTGAACTCCCCAGACCCACTGCTCTCCTCCTTTTCCCACCCTGATTCCCCA
                  ************************************************************

HsCD00516201      TCTTCCTCATCTCCACCATCCACCCAGGAGGCCTCTCCCACTGGTGATATGGCTGTAGGA
NM_002536.2       TCTTCCTCATCTCCACCATCCACCCAGGAGGCCTCTCCCACTGGTGATATGGCTGTAGGA
                  ************************************************************

HsCD00516201      TCCCCCTTGATGCAAGAGGTAGGCTCCCCGAAAGACCCTGGAAAGTCCCTGCCACCTGTA
NM_002536.2       TCCCCCTTGATGCAAGAGGTAGGCTCCCCGAAAGACCCTGGAAAGTCCCTGCCACCTGTA
                  ************************************************************

HsCD00516201      CCACCAATGGGCCTGCCCCCACCCCAGGAGTTTGGCCGGGGGAACCCATTCATGCTGTTC
NM_002536.2       CCACCAATGGGCCTGCCCCCACCCCAGGAGTTTGGCCGGGGGAACCCATTCATGCTGTTC
                  ************************************************************

HsCD00516201      CTCTGCCTGGCCATCCTGCTGGAGCACCGCGACCACATCATGCGCAATGGGCTGGATTAT
NM_002536.2       CTCTGCCTGGCCATCCTGCTGGAGCACCGCGACCACATCATGCGCAATGGGCTGGATTAT
                  ************************************************************

HsCD00516201      AATGAGCTGGCCATGCACTTTGACCGCCTTGTGCGAAAACACCACCTGGGGCGCGTCCTG
NM_002536.2       AATGAGCTGGCCATGCACTTTGACCGCCTTGTGCGAAAACACCACCTGGGGCGCGTCCTG
                  ************************************************************

HsCD00516201      CGCCGGGCTAGGGCTCTCTTTGCTGATTACCTGCAGTCAGAGGTGTGGGACTCAGAGGAG
NM_002536.2       CGCCGGGCTAGGGCTCTCTTTGCTGATTACCTGCAGTCAGAGGTGTGGGACTCAGAGGAG
                  ************************************************************

HsCD00516201      GGGGCTGAGGCCACAGCCGCATCT---
NM_002536.2       GGGGCTGAGGCCACAGCCGCATCTTGA
                  ************************