DNASU Plasmid | PARP12 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PARP12

Gene Name: poly (ADP-ribose) polymerase family, member 12

Original Clone ID:

ccsbBroadEn_08865

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC081541.1

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516208

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2103nts

Open reading frame: 1 to 2103

Reference Sequence Alignment

ATGGCCCAGGCCGGCGTCGTCGGTGAGGTCACCCAGGTGCTGTGCGCGGCCGGGGGCGCC
CTGGAGTTGCCCGAGCTGCGGCGCCGCTTGCGGATGGGCTTGAGCGCCGACGCGCTGGAG
CGGCTGCTGCGGCAGCGTGGGCGCTTCGTGGTGGCGGTGCGGGCGGGCGGCGCAGCCGCG
GCCCCGGAGCGCGTGGTGCTGGCCGCCTCGCCGCTGCGCCTGTGTCGCGCGCACCAGGGC
TCCAAGCCGGGCTGCGTGGGGCTCTGCGCGCAGCTCCACCTCTGCAGGTTCATGGTCTAC
GGCGCCTGCAAGTTCCTGAGAGCCGGGAAGAACTGTAGGAATAGTCACAGCTTGACAACC
GAACACAACCTGAGTGTGCTGAGAACTCATGGCGTTGACCACCTGAGCTATAATGAGCTA
TGCCAACTCTTGTTTCAGAACGACCCCTGGCTTTTGCCAGAAATTTGCCAACATTACAAC
AAAGGAGATGGACCCCACGGCTCTTGTGCCTTTCAAAAGCAGTGCATCAAGCTCCATATC
TGCCAGTATTTTTTACAGGGGGAATGCAAGTTTGGCACTAGCTGTAAGAGATCCCATGAT
TTCTCTAATTCTGAGAATCTGGAAAAATTGGAGAAGTTGGGTATGAGCTCAGACCTGGTG
AGCAGGCTGCCTACCATTTATAGAAATGCACATGACATCAAGAATAAGAGCTCTGCCCCC
AGCAGAGTGCCTCCTCTTTTTGTCCCACAGGGGACTTCTGAAAGAAAAGACAGTTCAGGT
TCTGTGTCCCCAAACACTCTTAGCCAGGAGGAGGGTGATCAGATCTGTTTGTACCATATC
CGGAAAAGTTGTAGCTTTCAAGATAAGTGCCATAGAGTTCATTTCCATTTGCCGTATCGA
TGGCAATTCTTGGATAGAGGCAAATGGGAGGATTTGGACAACATGGAACTTATTGAAGAG
GCATATTGCAATCCCAAAATAGAAAGGATCCTGTGCTCTGAGTCAGCCAGTACATTTCAC
TCTCATTGTCTGAACTTTAACGCCATGACTTACGGTGCTACCCAGGCTCGCCGCCTCTCC
ACGGCCTCCTCTGTCACCAAACCTCCACACTTCATCCTCACCACTGACTGGATTTGGTAC
TGGAGTGATGAGTTTGGTTCTTGGCAGGAATATGGAAGACAGGGCACGGTGCACCCTGTG
ACCACTGTCAGCAGTAGCGACGTGGAGAAGGCCTACCTGGCCTACTGTACACCGGGGTCT
GACGGCCAGGCAGCCACCTTGAAGTTCCAGGCCGGAAAGCACAACTACGAGTTAGATTTC
AAAGCCTTCGTTCAGAAAAACCTGGTCTATGGCACAACTAAAAAGGTTTGCCGCAGACCC
AAATACGTGTCTCCCCAGGATGTGACGACCATGCAAACCTGCAATACCAAGTTTCCAGGC
CCGAAGAGCATCCCAGACTATTGGGACTCCTCTGCCCTGCCAGACCCAGGCTTTCAGAAG
ATCACCCTTAGTTCTTCCTCGGAAGAGTATCAGAAGGTCTGGAACCTCTTTAACCGCACG
CTGCCTTTCTACTTTGTTCAGAAGATTGAGCGAGTACAGAACCTGGCCCTCTGGGAAGTC
TACCAGTGGCAAAAAGGACAGATGCAGAAGCAGAATGGAGGGAAGGCCGTGGACGAGCGG
CAGCTGTTCCACGGCACCAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGAC
TGGCGGGTCTGTGGTGTTCATGGCACTTCCTACGGCAAGGGGAGCTACTTTGCCCGAGAT
GCTGCATATTCCCACCACTACAGCAAATCCGACACGCAGACCCACACGATGTTCCTGGCC
CGGGTGCTGGTGGGCGAGTTCGTCAGGGGCAATGCGTCCTTTGTCCGTCCGCCGGCCAAG
GAGGGCTGGAGCAACGCCTTCTATGATAGCTGCGTGAACAGTGTGTCCGACCCCTCCATC
TTTGTGATCTTTGAGAAACACCAGGTCTACCCAGAGTATGTCATCCAGTACACCACCTCC
TCCAAGCCCTCGGTCACACCCTCCATCCTGCTGGCCTTGGGCTCCCTGTTCAGCAGCCGA
CAG

Protein sequence predicted based on nucleotide sequence

MAQAGVVGEVTQVLCAAGGALELPELRRRLRMGLSADALERLLRQRGRFVVAVRAGGAAA
APERVVLAASPLRLCRAHQGSKPGCVGLCAQLHLCRFMVYGACKFLRAGKNCRNSHSLTT
EHNLSVLRTHGVDHLSYNELCQLLFQNDPWLLPEICQHYNKGDGPHGSCAFQKQCIKLHI
CQYFLQGECKFGTSCKRSHDFSNSENLEKLEKLGMSSDLVSRLPTIYRNAHDIKNKSSAP
SRVPPLFVPQGTSERKDSSGSVSPNTLSQEEGDQICLYHIRKSCSFQDKCHRVHFHLPYR
WQFLDRGKWEDLDNMELIEEAYCNPKIERILCSESASTFHSHCLNFNAMTYGATQARRLS
TASSVTKPPHFILTTDWIWYWSDEFGSWQEYGRQGTVHPVTTVSSSDVEKAYLAYCTPGS
DGQAATLKFQAGKHNYELDFKAFVQKNLVYGTTKKVCRRPKYVSPQDVTTMQTCNTKFPG
PKSIPDYWDSSALPDPGFQKITLSSSSEEYQKVWNLFNRTLPFYFVQKIERVQNLALWEV
YQWQKGQMQKQNGGKAVDERQLFHGTSAIFVDAICQQNFDWRVCGVHGTSYGKGSYFARD
AAYSHHYSKSDTQTHTMFLARVLVGEFVRGNASFVRPPAKEGWSNAFYDSCVNSVSDPSI
FVIFEKHQVYPEYVIQYTTSSKPSVTPSILLALGSLFSSRQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

1 error

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PARP12

Symbol Nomenclature :

PARP12

SYNONYM :

ARTD12; MST109; MSTP109; ZC3H1; ZC3HDC1

Designation :

ADP-ribosyltransferase diphtheria toxin-like 12|poly [ADP-ribose] polymerase 12|zinc finger CCCH domain-containing protein 1|zinc finger CCCH type domain containing 1

Full Nomenclature :

poly (ADP-ribose) polymerase family, member 12

GENEID :

64761

HGNC :

21919

MIM :

612481

Vega :

OTTHUMG00000157315

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516208      ATGGCCCAGGCCGGCGTCGTCGGTGAGGTCACCCAGGTGCTGTGCGCGGCCGGGGGCGCC
NM_022750.2       ATGGCCCAGGCCGGCGTCGTCGGTGAGGTCACCCAGGTGCTGTGCGCGGCCGGGGGCGCC
                  ************************************************************

HsCD00516208      CTGGAGTTGCCCGAGCTGCGGCGCCGCTTGCGGATGGGCTTGAGCGCCGACGCGCTGGAG
NM_022750.2       CTGGAGTTGCCCGAGCTGCGGCGCCGCTTGCGGATGGGCTTGAGCGCCGACGCGCTGGAG
                  ************************************************************

HsCD00516208      CGGCTGCTGCGGCAGCGTGGGCGCTTCGTGGTGGCGGTGCGGGCGGGCGGCGCAGCCGCG
NM_022750.2       CGGCTGCTGCGGCAGCGTGGGCGCTTCGTGGTGGCGGTGCGGGCGGGCGGCGCAGCCGCG
                  ************************************************************

HsCD00516208      GCCCCGGAGCGCGTGGTGCTGGCCGCCTCGCCGCTGCGCCTGTGTCGCGCGCACCAGGGC
NM_022750.2       GCCCCGGAGCGCGTGGTGCTGGCCGCCTCGCCGCTGCGCCTGTGTCGCGCGCACCAGGGC
                  ************************************************************

HsCD00516208      TCCAAGCCGGGCTGCGTGGGGCTCTGCGCGCAGCTCCACCTCTGCAGGTTCATGGTCTAC
NM_022750.2       TCCAAGCCGGGCTGCGTGGGGCTCTGCGCGCAGCTCCACCTCTGCAGGTTCATGGTCTAC
                  ************************************************************

HsCD00516208      GGCGCCTGCAAGTTCCTGAGAGCCGGGAAGAACTGTAGGAATAGTCACAGCTTGACAACC
NM_022750.2       GGCGCCTGCAAGTTCCTGAGAGCCGGGAAGAACTGTAGGAATAGTCACAGCTTGACAACC
                  ************************************************************

HsCD00516208      GAACACAACCTGAGTGTGCTGAGAACTCATGGCGTTGACCACCTGAGCTATAATGAGCTA
NM_022750.2       GAACACAACCTGAGTGTGCTGAGAACTCATGGCGTTGACCACCTGAGCTATAATGAGCTA
                  ************************************************************

HsCD00516208      TGCCAACTCTTGTTTCAGAACGACCCCTGGCTTTTGCCAGAAATTTGCCAACATTACAAC
NM_022750.2       TGCCAACTCTTGTTTCAGAACGACCCCTGGCTTTTGCCAGAAATTTGCCAACATTACAAC
                  ************************************************************

HsCD00516208      AAAGGAGATGGACCCCACGGCTCTTGTGCCTTTCAAAAGCAGTGCATCAAGCTCCATATC
NM_022750.2       AAAGGAGATGGACCCCACGGCTCTTGTGCCTTTCAAAAGCAGTGCATCAAGCTCCATATC
                  ************************************************************

HsCD00516208      TGCCAGTATTTTTTACAGGGGGAATGCAAGTTTGGCACTAGCTGTAAGAGATCCCATGAT
NM_022750.2       TGCCAGTATTTTTTACAGGGGGAATGCAAGTTTGGCACTAGCTGTAAGAGATCCCATGAT
                  ************************************************************

HsCD00516208      TTCTCTAATTCTGAGAATCTGGAAAAATTGGAGAAGTTGGGTATGAGCTCAGACCTGGTG
NM_022750.2       TTCTCTAATTCTGAGAATCTGGAAAAATTGGAGAAGTTGGGTATGAGCTCAGACCTGGTG
                  ************************************************************

HsCD00516208      AGCAGGCTGCCTACCATTTATAGAAATGCACATGACATCAAGAATAAGAGCTCTGCCCCC
NM_022750.2       AGCAGGCTGCCTACCATTTATAGAAATGCACATGACATCAAGAATAAGAGCTCTGCCCCC
                  ************************************************************

HsCD00516208      AGCAGAGTGCCTCCTCTTTTTGTCCCACAGGGGACTTCTGAAAGAAAAGACAGTTCAGGT
NM_022750.2       AGCAGAGTGCCTCCTCTTTTTGTCCCACAGGGGACTTCTGAAAGAAAAGACAGTTCAGGT
                  ************************************************************

HsCD00516208      TCTGTGTCCCCAAACACTCTTAGCCAGGAGGAGGGTGATCAGATCTGTTTGTACCATATC
NM_022750.2       TCTGTGTCCCCAAACACTCTTAGCCAGGAGGAGGGTGATCAGATCTGTTTGTACCATATC
                  ************************************************************

HsCD00516208      CGGAAAAGTTGTAGCTTTCAAGATAAGTGCCATAGAGTTCATTTCCATTTGCCGTATCGA
NM_022750.2       CGGAAAAGTTGTAGCTTTCAAGATAAGTGCCATAGAGTTCATTTCCATTTGCCGTATCGA
                  ************************************************************

HsCD00516208      TGGCAATTCTTGGATAGAGGCAAATGGGAGGATTTGGACAACATGGAACTTATTGAAGAG
NM_022750.2       TGGCAATTCTTGGATAGAGGCAAATGGGAGGATTTGGACAACATGGAACTTATTGAAGAG
                  ************************************************************

HsCD00516208      GCATATTGCAATCCCAAAATAGAAAGGATCCTGTGCTCTGAGTCAGCCAGTACATTTCAC
NM_022750.2       GCATATTGCAATCCCAAAATAGAAAGGATCCTGTGCTCTGAGTCAGCCAGTACCTTTCAC
                  *****************************************************.******

HsCD00516208      TCTCATTGTCTGAACTTTAACGCCATGACTTACGGTGCTACCCAGGCTCGCCGCCTCTCC
NM_022750.2       TCTCATTGTCTGAACTTTAACGCCATGACTTACGGTGCTACCCAGGCTCGCCGCCTCTCC
                  ************************************************************

HsCD00516208      ACGGCCTCCTCTGTCACCAAACCTCCACACTTCATCCTCACCACTGACTGGATTTGGTAC
NM_022750.2       ACGGCCTCCTCTGTCACCAAACCTCCACACTTCATCCTCACCACTGACTGGATTTGGTAC
                  ************************************************************

HsCD00516208      TGGAGTGATGAGTTTGGTTCTTGGCAGGAATATGGAAGACAGGGCACGGTGCACCCTGTG
NM_022750.2       TGGAGTGATGAGTTTGGTTCTTGGCAGGAATATGGAAGACAGGGCACGGTGCACCCTGTG
                  ************************************************************

HsCD00516208      ACCACTGTCAGCAGTAGCGACGTGGAGAAGGCCTACCTGGCCTACTGTACACCGGGGTCT
NM_022750.2       ACCACTGTCAGCAGTAGCGACGTGGAGAAGGCCTACCTGGCCTACTGTACACCGGGGTCT
                  ************************************************************

HsCD00516208      GACGGCCAGGCAGCCACCTTGAAGTTCCAGGCCGGAAAGCACAACTACGAGTTAGATTTC
NM_022750.2       GACGGCCAGGCAGCCACCTTGAAGTTCCAGGCCGGAAAGCACAACTACGAGTTAGATTTC
                  ************************************************************

HsCD00516208      AAAGCCTTCGTTCAGAAAAACCTGGTCTATGGCACAACTAAAAAGGTTTGCCGCAGACCC
NM_022750.2       AAAGCCTTCGTTCAGAAAAACCTGGTCTATGGCACAACTAAAAAGGTTTGCCGCAGACCC
                  ************************************************************

HsCD00516208      AAATACGTGTCTCCCCAGGATGTGACGACCATGCAAACCTGCAATACCAAGTTTCCAGGC
NM_022750.2       AAATACGTGTCTCCCCAGGATGTGACGACCATGCAAACCTGCAATACCAAGTTTCCAGGC
                  ************************************************************

HsCD00516208      CCGAAGAGCATCCCAGACTATTGGGACTCCTCTGCCCTGCCAGACCCAGGCTTTCAGAAG
NM_022750.2       CCGAAGAGCATCCCAGACTATTGGGACTCCTCTGCCCTGCCAGACCCAGGCTTTCAGAAG
                  ************************************************************

HsCD00516208      ATCACCCTTAGTTCTTCCTCGGAAGAGTATCAGAAGGTCTGGAACCTCTTTAACCGCACG
NM_022750.2       ATCACCCTTAGTTCTTCCTCGGAAGAGTATCAGAAGGTCTGGAACCTCTTTAACCGCACG
                  ************************************************************

HsCD00516208      CTGCCTTTCTACTTTGTTCAGAAGATTGAGCGAGTACAGAACCTGGCCCTCTGGGAAGTC
NM_022750.2       CTGCCTTTCTACTTTGTTCAGAAGATTGAGCGAGTACAGAACCTGGCCCTCTGGGAAGTC
                  ************************************************************

HsCD00516208      TACCAGTGGCAAAAAGGACAGATGCAGAAGCAGAATGGAGGGAAGGCCGTGGACGAGCGG
NM_022750.2       TACCAGTGGCAAAAAGGACAGATGCAGAAGCAGAATGGAGGGAAGGCCGTGGACGAGCGG
                  ************************************************************

HsCD00516208      CAGCTGTTCCACGGCACCAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGAC
NM_022750.2       CAGCTGTTCCACGGCACCAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGAC
                  ************************************************************

HsCD00516208      TGGCGGGTCTGTGGTGTTCATGGCACTTCCTACGGCAAGGGGAGCTACTTTGCCCGAGAT
NM_022750.2       TGGCGGGTCTGTGGTGTTCATGGCACTTCCTACGGCAAGGGGAGCTACTTTGCCCGAGAT
                  ************************************************************

HsCD00516208      GCTGCATATTCCCACCACTACAGCAAATCCGACACGCAGACCCACACGATGTTCCTGGCC
NM_022750.2       GCTGCATATTCCCACCACTACAGCAAATCCGACACGCAGACCCACACGATGTTCCTGGCC
                  ************************************************************

HsCD00516208      CGGGTGCTGGTGGGCGAGTTCGTCAGGGGCAATGCGTCCTTTGTCCGTCCGCCGGCCAAG
NM_022750.2       CGGGTGCTGGTGGGCGAGTTCGTCAGGGGCAATGCGTCCTTTGTCCGTCCGCCGGCCAAG
                  ************************************************************

HsCD00516208      GAGGGCTGGAGCAACGCCTTCTATGATAGCTGCGTGAACAGTGTGTCCGACCCCTCCATC
NM_022750.2       GAGGGCTGGAGCAACGCCTTCTATGATAGCTGCGTGAACAGTGTGTCCGACCCCTCCATC
                  ************************************************************

HsCD00516208      TTTGTGATCTTTGAGAAACACCAGGTCTACCCAGAGTATGTCATCCAGTACACCACCTCC
NM_022750.2       TTTGTGATCTTTGAGAAACACCAGGTCTACCCAGAGTATGTCATCCAGTACACCACCTCC
                  ************************************************************

HsCD00516208      TCCAAGCCCTCGGTCACACCCTCCATCCTGCTGGCCTTGGGCTCCCTGTTCAGCAGCCGA
NM_022750.2       TCCAAGCCCTCGGTCACACCCTCCATCCTGCTGGCCTTGGGCTCCCTGTTCAGCAGCCGA
                  ************************************************************

HsCD00516208      CAG---
NM_022750.2       CAGTGA
                  ***