Gene:
Gene Symbol:
MARCH7
Gene Name:
membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
Original Clone ID:
ccsbBroadEn_08875
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC065014.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516211
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGTCTAAACCTTCAAGGATTCCAAGAAGAATTTCTGTTCAACCTTCCAGCTCCTTA
AGTGCTAGGATGATGTCTGGAAGCAGAGGAAGTAGTTTAAATGATACCTATCACTCAAGA
GACTCTTCATTTAGATTGGATTCTGAATATCAGTCTACATCAGCATCAGCATCTGCGTCA
CCATTTCAATCTGCATGGTATAGTGAATCTGAGATAACTCAGGGAGCACGCTCAAGATCG
CAGAACCAGCAACGGGATCATGATTCAAAAAGACCTAAACTTTCCTGTACAAACTGTACT
ACCTCAGCTGGGAGAAATGTTGGAAATGGTTTAAACACATTATCAGATTCATCTTGGAGG
CATAGTCAAGTTCCTAGATCTTCATCAATGGTACTTGGATCATTTGGAACAGACTTAATG
AGAGAGAGGAGAGATTTGGAGAGAAGAACAGATTCCTCTATTAGTAATCTTATGGATTAT
AGTCACCGAAGTGGTGATTTCACAACTTCATCATATGTTCAAGACAGAGTTCCTTCATAT
TCACAAGGAGCAAGACCAAAAGAAAACTCAATGAGCACTTTACAGTTGAATACATCATCC
ACAAACCACCAATTGCCTTCTGAACATCAGACCATACTAAGTTCTAGGGATTCCAGAAAT
TCTTTAAGATCAAATTTTTCTTCAAGAGAATCAGAATCTTCCCGAAGCAATACGCAGCCT
GGATTTTCTTACAGTTCAAGTAGAGATGAAGCCCCAATCATAAGCAATTCAGAAAGGGTT
GTTTCATCTCAAAGACCATTTCAAGAATCTTCTGACAATGAAGGTAGGCGGACAACGAGG
AGATTGCTGTCACGCATAGCTTCTAGCATGTCATCTACTTTTTTTTCACGAAGATCTAGT
CAGGATTCCTTGAATACAAGATCATTGAATTCTGAAAATTCTTACGTTTCTCCAAGAATC
TTGACAGCTTCACAGTCCCGTAGTAATGTACCATCAGCTTCTGAAGTTCCCGATAATAGG
GCATCTGAAGCTTCTCAGGGATTTCGATTTCTTAGGCGAAGATGGGGTTTGTCATCTCTT
AGCCACAATCATAGCTCTGAGTCAGATTCAGAAAATTTTAACCAAGAATCTGAAGGTAGA
AATACAGGACCATGGTTATCTTCCTCACTTAGAAATAGATGCACACCTTTGTTCTCTAGA
AGGAGGCGAGAGGGAAGAGATGAATCTTCAAGGATACCTACCTCTGATACATCATCTAGA
TCTCATATTTTTAGAAGAGAATCAAATGAAGTGGTTCACCTTGAAGCACAGAATGATCCT
CTTGGAGCTGCTGCCAACAGACCACAAGCATCTGCAGCATCAAGCAGTGCCACAACAGGT
GGCTCTACATCAGATTCGGCTCAAGGTGGAAGAAATACAGGAATATCAGGGATTCTTCCT
GGTTCCTTATTCCGGTTTGCAGTCCCCCCAGCACTTGGGAGTAATTTGACCGACAATGTC
ATGATCACAGTAGATATTATTCCTTCAGGTTGGAATTCAGCTGATGGTAAAAGTGATAAA
ACTAAAAGTGCGCCTTCAAGAGATCCAGAAAGATTGCAGAAAATAAAAGAGAGCCTCCTT
TTAGAGGACTCAGAAGAAGAAGAAGGTGACTTATGTAGAATTTGTCAAATGGCAGCTGCA
TCATCATCTAATTTGCTGATAGAGCCATGCAAGTGCACAGGAAGTTTGCAGTATGTCCAC
CAAGACTGTATGAAAAAGTGGTTACAGGCCAAAATTAACTCTGGTTCTTCATTAGAAGCT
GTAACCACCTGTGAACTATGTAAAGAGAAGTTGGAGCTTAACCTGGAGGATTTTGATATT
CATGAACTACATAGAGCTCATGCAAATGAACAAGCTGAGTATGAGTTTATCAGCTCTGGT
CTCTACCTAGTGGTGTTATTGCACTTGTGCGAACAAAGCTTTTCTGATATGATGGGAAAT
ACAAATGAACCAAGCACACGTGTCCGATTTATTAACCTTGCAAGAACTCTTCAGGCACAT
ATGGAAGATCTCGAAACTTCAGAGGATGATTCCGAAGAAGACGGAGACCATAACAGGACA
TTTGATATTGCC
Protein sequence predicted based on nucleotide sequence
MESKPSRIPRRISVQPSSSLSARMMSGSRGSSLNDTYHSRDSSFRLDSEYQSTSASASAS PFQSAWYSESEITQGARSRSQNQQRDHDSKRPKLSCTNCTTSAGRNVGNGLNTLSDSSWR HSQVPRSSSMVLGSFGTDLMRERRDLERRTDSSISNLMDYSHRSGDFTTSSYVQDRVPSY SQGARPKENSMSTLQLNTSSTNHQLPSEHQTILSSRDSRNSLRSNFSSRESESSRSNTQP GFSYSSSRDEAPIISNSERVVSSQRPFQESSDNEGRRTTRRLLSRIASSMSSTFFSRRSS QDSLNTRSLNSENSYVSPRILTASQSRSNVPSASEVPDNRASEASQGFRFLRRRWGLSSL SHNHSSESDSENFNQESEGRNTGPWLSSSLRNRCTPLFSRRRREGRDESSRIPTSDTSSR SHIFRRESNEVVHLEAQNDPLGAAANRPQASAASSSATTGGSTSDSAQGGRNTGISGILP GSLFRFAVPPALGSNLTDNVMITVDIIPSGWNSADGKSDKTKSAPSRDPERLQKIKESLL LEDSEEEEGDLCRICQMAAASSSNLLIEPCKCTGSLQYVHQDCMKKWLQAKINSGSSLEA VTTCELCKEKLELNLEDFDIHELHRAHANEQAEYEFISSGLYLVVLLHLCEQSFSDMMGN TNEPSTRVRFINLARTLQAHMEDLETSEDDSEEDGDHNRTFDIA
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
