RFWD2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  RFWD2
Gene Name:  ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
Original Clone ID:
ccsbBroadEn_03940
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC094728.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516231
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2193nts
Open reading frame: 1 to 2193

ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCG
GCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTTCCGTG
GCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGG
GCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCGTAGGA
GGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTT
GATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATT
CATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTT
GAGGAAAAGAGGTTCAAATTGGACCACTCAGTGAGTAGCACCAATGGCCACAGGTGGCAG
ATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTT
ATGTTGGAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAA
CTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAA
CAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGT
GGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCA
TCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGT
TCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTT
ACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCA
GATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACT
CGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGT
TCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGA
GTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATT
CATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGT
TACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGA
TTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGAC
TTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTG
TGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGT
GTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTC
CACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAA
GCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGT
CAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATC
AATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAA
AATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTT
GATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTT
AGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAAC
AGTCAGGGTACAATTAAGGTGCTAGAATTGGTA

Protein sequence predicted based on nucleotide sequence

MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGG
LGGPVRPVLVAPAVSGSGGGAVSTGLSRHSCAARPSAGVGGSSSSLGSGSRKRPLLAPLC
NGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNI
DHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNL
MLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMS
GLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRL
TAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNG
SSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKISCISWSS
YHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKL
WSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRK
AVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSE
NNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAAN
SQGTIKVLELV

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable