MKLN1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MKLN1
Gene Name:  muskelin 1, intracellular mediator containing kelch motifs
Original Clone ID:
ccsbBroadEn_06584
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC067825.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516233
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2205nts
Open reading frame: 1 to 2205

ATGGCGGCTGGCGGAGCTGTCGCTGCGGCGCCCGAGTGCCGGCTTCTCCCCTACGCGCTA
CACAAGTGGAGCTCCTTTTCCTCCACCTACCTTCCCGAGAACATTTTAGTGGACAAACCA
AATGACCAATCTTCAAGATGGTCTTCAGAGAGCAACTATCCTCCCCAGTACTTGATTCTA
AAGCTCGAAAGGCCTGCTATAGTTCAGAATATCACATTTGGAAAATATGAGAAAACTCAT
GTTTGCAATTTGAAGAAATTTAAAGTCTTTGGTGGAATGAATGAAGAAAATATGACAGAG
CTGTTGTCCAGTGGCTTAAAGAATGATTATAACAAAGAAACATTCACCTTGAAGCATAAA
ATTGATGAACAGATGTTCCCTTGTCGATTCATTAAAATAGTTCCACTCTTGTCCTGGGGA
CCCAGCTTTAACTTTAGCATCTGGTATGTTGAACTTAGTGGCATTGATGATCCTGATATA
GTACAACCTTGTCTCAACTGGTATAGCAAGTACCGTGAACAGGAAGCTATTCGCCTTTGC
CTAAAACACTTCAGACAACACAACTATACAGAAGCTTTTGAGTCACTGCAAAAGAAAACC
AAGATTGCACTGGAACATCCCATGTTAACAGATATTCATGACAAGCTGGTGTTGAAGGGT
GATTTTGATGCTTGCGAAGAGTTGATTGAAAAGGCTGTAAATGATGGCTTGTTCAATCAG
TATATCAGTCAACAGGAATATAAGCCACGATGGAGTCAAATCATTCCCAAAAGTACCAAA
GGTGATGGGGAAGATAACCGTCCAGGAATGAGAGGAGGCCATCAGATGGTTATTGATGTT
CAAACAGAGACTGTTTATTTGTTTGGTGGCTGGGATGGAACACAAGATCTTGCTGACTTC
TGGGCGTACAGTGTGAAGGAGAACCAGTGGACATGTATCTCTAGAGACACTGAAAAAGAG
AATGGTCCTAGTGCCAGATCGTGTCATAAAATGTGCATTGATATTCAACGGAGGCAAATC
TACACATTGGGGCGTTACTTGGATTCCTCTGTGAGGAACAGCAAATCTCTGAAAAGTGAC
TTCTATCGTTATGACATTGATACAAACACATGGATGTTACTAAGTGAGGATACTGCTGCT
GATGGAGGGCCGAAATTGGTGTTTGATCATCAGATGTGTATGGACTCAGAAAAACATATG
ATCTACACTTTTGGTGGTAGAATTTTGACTTGTAATGGCAGCGTAGATGACAGCAGAGCC
AGTGAACCACAATTCAGTGGCTTGTTTGCTTTCAACTGTCAATGTCAAACCTGGAAACTT
CTTAGAGAGGACTCCTGTAATGCTGGGCCTGAGGACATCCAGTCTCGAATAGGACACTGC
ATGTTATTCCACTCAAAAAATCGTTGCTTATATGTATTTGGTGGCCAGCGATCAAAGACC
TATTTGAATGATTTCTTTAGTTATGATGTGGACTCTGATCATGTAGACATAATATCAGAT
GGCACCAAGAAAGACTCTGGGATGGTTCCAATGACAGGATTTACACAGAGAGCAACTATT
GATCCAGAACTGAACGAAATACACGTCTTATCTGGACTCAGCAAAGATAAGGAAAAGAGG
GAAGAAAATGTTAGAAATTCATTCTGGATTTATGACATTGTGAGGAATAGTTGGTCTTGT
GTCTATAAGAATGATCAAGCTGCAAAGGATAATCCAACTAAAAGTCTTCAGGAAGAAGAA
CCATGTCCAAGGTTTGCCCATCAGCTTGTATACGATGAGCTACACAAGGTTCATTACTTA
TTTGGTGGGAATCCAGGAAAATCTTGCTCTCCAAAGATGAGATTAGATGACTTCTGGTCA
CTGAAGTTGTGTAGACCTTCAAAAGATTATTTACTGAGGCATTGCAAGTACCTCATAAGA
AAACACAGGTTTGAAGAAAAGGCCCAAGTGGATCCCCTTAGTGCTCTGAAATATTTACAA
AATGATCTTTATATAACTGTGGATCATTCAGACCCAGAAGAGACAAAAGAGTTTCAGCTC
CTGGCATCAGCTCTATTCAAATCTGGTTCAGATTTTACAGCTCTGGGCTTTTCTGATGTG
GATCACACCTATGCTCAAAGAACTCAGCTCTTTGACACCTTAGTAAATTTCTTTCCTGAC
AGCATGACTCCTCCTAAAGGCAACCTGGTAGACCTCATCACACTG

Protein sequence predicted based on nucleotide sequence

MAAGGAVAAAPECRLLPYALHKWSSFSSTYLPENILVDKPNDQSSRWSSESNYPPQYLIL
KLERPAIVQNITFGKYEKTHVCNLKKFKVFGGMNEENMTELLSSGLKNDYNKETFTLKHK
IDEQMFPCRFIKIVPLLSWGPSFNFSIWYVELSGIDDPDIVQPCLNWYSKYREQEAIRLC
LKHFRQHNYTEAFESLQKKTKIALEHPMLTDIHDKLVLKGDFDACEELIEKAVNDGLFNQ
YISQQEYKPRWSQIIPKSTKGDGEDNRPGMRGGHQMVIDVQTETVYLFGGWDGTQDLADF
WAYSVKENQWTCISRDTEKENGPSARSCHKMCIDIQRRQIYTLGRYLDSSVRNSKSLKSD
FYRYDIDTNTWMLLSEDTAADGGPKLVFDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRA
SEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKT
YLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKR
EENVRNSFWIYDIVRNSWSCVYKNDQAAKDNPTKSLQEEEPCPRFAHQLVYDELHKVHYL
FGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQVDPLSALKYLQ
NDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPD
SMTPPKGNLVDLITL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable