DENND2A (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DENND2A
Gene Name:  DENN/MADD domain containing 2A
Original Clone ID:
ccsbBroadEn_11848
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC115004.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516284
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2386nts
Open reading frame: 1 to 2386

ATGGATATGTTCAGCTTGGATATGATCATCAGTGACCCAGCTGCAGAAGCCAGCAGGGCT
GGGAAGAAGCAGCTCAGAGGTGTTCAGAACCCTTGCCCATCTGCCAGAGCCAGACCCCGG
CACAAGTCCCTCAACATAAAGGACAAGATATCAGAATGGGAAGGGAAGAAAGAGGTGCCC
ACTCCTGCACCCAGCAGGAGAGCAGACGGACAGGAGGATTATCTGCCGTCCTCTACGGTG
GAGAGGAGGAGTAGTGATGGGGTGAGAACTCAGGTCACAGAGGCTAAGAATGGAATGAGG
CCAGGAACAGAGAGCACAGAGAAGGAGAGGAATAAAGGAGCAGTGAACGTCGGGGGACAG
GACCCAGAGCCGGGGCAAGACCTAAGCCAGCCAGAACGGGAAGTGGATCCTAGCTGGGGC
CGAGGCCGAGAGCCAAGACTTGGCAAGCTACGCTTTCAGAACGATCACCTCTCCGTGCTG
AAGCAGGTCAAGAAACTCGAGCAGGCTTTGAAGGATGGGTCGGCAGGGCTGGATCCCCAG
TTACCAGGGACTTGTTACTCCCCACACTGCCCTCCTGACAAGGCAGAGGCAGGGTCCACC
CTTCCTGAGAACCTGGGAGGCGGGAGTGGCTCAGAAGTCAGCCAGAGGGTCCACCCCTCG
GACCTGGAAGGCAGGGAGCCCACCCCTGAGCTTGTGGAGGACAGGAAAGGTTCATGCAGA
AGGCCCTGGGACCGGAGCCTTGAGAACGTGTATAGGGGCTCGGAGGGTTCCCCCACAAAG
CCCTTCATCAACCCTCTGCCAAAACCCCGGAGAACGTTCAAACATGCCGGAGAAGGGGAC
AAAGATGGGAAGCCTGGCATCGGCTTCAGGAAAGAGAAAAGAAATCTGCCTCCTCTGCCC
TCTCTACCTCCCCCGCCTCTGCCCTCCTCTCCCCCACCTTCCTCTGTGAACAGAAGACTG
TGGACCGGGAGACAGAAATCCAGTGCAGACCACAGAAAGTCCTATGAGTTTGAAGATTTA
CTGCAGTCTTCCTCTGAGAGCAGCAGGGTGGACTGGTACGCGCAGACTAAGCTGGGGCTG
ACACGCACTTTATCGGAGGAGAACGTCTATGAAGACATTCTAGATCCGCCAATGAAGGAG
AACCCTTATGAGGACATCGAGTTACATGGTCGCTGCCTGGGAAAGAAGTGTGTCTTGAAT
TTTCCTGCTTCTCCCACCTCTTCCATCCCTGACACACTCACCAAGCAGTCATTGTCCAAA
CCTGCTTTTTTCCGACAAAATTCAGAGAGGAGGAACTTCAAGCTGCTGGACACTAGGAAG
CTGAGTCGGGATGGAACTGGGTCCCCTTCCAAAATCAGCCCTCCCTCCACTCCCAGCAGC
CCTGATGACATTTTCTTTAACCTTGGAGACCCACAGAACGGCAGGAAGAAGAGAAAGATA
CCCAAGCTGGTGTTGCGAATCAACGCCATTTATGAGGTCCGGAGAGGAAAGAAACGGGTG
AAGAGGCTGTCCCAGTCAATGGAGAGCAACTCAGGAAAAGTGACAGATGAGAACAGTGAG
TCTGACAGTGACACAGAGGAGAAGCTGAAAGCTCACAGCCAGCGCCTGGTCAACGTGAAG
TCCCGGCTGAAGCAGGCGCCTCGGTACCCATCACTTGCCCGGGAACTCATCGAGTACCAG
GAGAGGCAGCTCTTCGAGTACTTTGTGGTTGTGTCTTTGCACAAGAAGCAGGCCGGGGCT
GCCTACGTGCCAGAACTCACCCAACAGTTCCCTCTGAAGTTGGAAAGGTCTTTCAAGTTC
ATGAGAGAAGCTGAGGACCAACTGAAGGCCATTCCCCAGTTCTGTTTTCCCGATGCCAAG
GATTGGGTTCCTGTCCAGCAGTTCACCAGTGAAACATTCTCATTTGTCTTAACTGGAGAA
GATGGGAGCAGAAGGTTCGGTTACTGCCGAAGACTGCTGCCTGGAGGCAAAGGGAAGCGC
CTTCCTGAAGTTTACTGCATTGTGAGCCGCCTGGGATGCTTCAGCCTCTTTTCAAGGATC
TTGGATGAGGTGGAAAAAAGACGAGGCATCTCTCCTGCCCTGGTTCAGCCACTCATGAGA
AGTGTCATGGAAGCCCCTTTCCCAGCCCTGGGCAAAACCATCCTTGTCAAGAACTTCCTG
CCAGGTTCAGGAACTGAGGTGATCGAACTGTGCCGCCCGCTGGACTCCCGGCTCGAGCAC
GTGGACTTTGAGTCTCTCTTCTCCTCCCTCAGCGTCCGCCACCTGGTCTGTGTGTTTGCC
TCCCTGCTTCTGGAGAGGAGGGTCATCTTCATTGCAGACAAGCTCAGGTACCCGCCTTGG
CTACTTCTGCTAAAGCGTTTGAATGACAGTCGCTCTTGGACCCTC

Protein sequence predicted based on nucleotide sequence

MDMFSLDMIISDPAAEASRAGKKQLRGVQNPCPSARARPRHKSLNIKDKISEWEGKKEVP
TPAPSRRADGQEDYLPSSTVERRSSDGVRTQVTEAKNGMRPGTESTEKERNKGAVNVGGQ
DPEPGQDLSQPEREVDPSWGRGREPRLGKLRFQNDHLSVLKQVKKLEQALKDGSAGLDPQ
LPGTCYSPHCPPDKAEAGSTLPENLGGGSGSEVSQRVHPSDLEGREPTPELVEDRKGSCR
RPWDRSLENVYRGSEGSPTKPFINPLPKPRRTFKHAGEGDKDGKPGIGFRKEKRNLPPLP
SLPPPPLPSSPPPSSVNRRLWTGRQKSSADHRKSYEFEDLLQSSSESSRVDWYAQTKLGL
TRTLSEENVYEDILDPPMKENPYEDIELHGRCLGKKCVLNFPASPTSSIPDTLTKQSLSK
PAFFRQNSERRNFKLLDTRKLSRDGTGSPSKISPPSTPSSPDDIFFNLGDPQNGRKKRKI
PKLVLRINAIYEVRRGKKRVKRLSQSMESNSGKVTDENSESDSDTEEKLKAHSQRLVNVK
SRLKQAPRYPSLARELIEYQERQLFEYFVVVSLHKKQAGAAYVPELTQQFPLKLERSFKF
MREAEDQLKAIPQFCFPDAKDWVPVQQFTSETFSFVLTGEDGSRRFGYCRRLLPGGKGKR
LPEVYCIVSRLGCFSLFSRILDEVEKRRGISPALVQPLMRSVMEAPFPALGKTILVKNFL
PGSGTEVIELCRPLDSRLEHVDFESLFSSLSVRHLVCVFASLLLERRVIFIADKLRYPPW
LLLLKRLNDSRSWTL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable