Gene:
Gene Symbol:
RBM25
Gene Name:
RNA binding motif protein 25
Original Clone ID:
ccsbBroadEn_03868
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC136775.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516331
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCTTTTCCACCTCATTTGAATCGCCCTCCCATGGGAATCCCAGCACTCCCACCAGGG
ATCCCACCCCCGCAGTTTCCAGGATTTCCTCCACCTGTACCTCCAGGGACCCCAATGATT
CCTGTACCAATGAGCATTATGGCTCCTGCTCCAACTGTCTTAGTACCCACTGTGTCTATG
GTTGGAAAGCATTTGGGCGCAAGAAAGGATCATCCAGGCTTAAAGGCTAAAGAAAATGAT
GAAAATTGTGGTCCTACTACCACTGTTTTTGTTGGCAACATTTCCGAGAAAGCTTCAGAC
ATGCTTATAAGACAACTCTTAGCTAAATGTGGTTTGGTTTTGAGCTGGAAGAGAGTACAA
GGTGCTTCCGGAAAGCTTCAAGCCTTCGGATTCTGTGAGTACAAGGAGCCAGAATCTACC
CTCCGTGCACTCAGATTATTACATGACCTGCAAATTGGAGAGAAAAAGCTACTCGTTAAA
GTTGATGCAAAGACAAAGGCACAGCTGGATGAATGGAAAGCAAAGAAGAAAGCTTCTAAT
GGGAATGCAAGGCCAGAAACTGTCACTAATGACGATGAAGAAGCCTTGGATGAAGAAACA
AAGAGGAGAGATCAGATGATTAAAGGGGCTATTGAAGTTTTAATTCGTGAATACTCCAGT
GAGCTAAATGCCCCCTCACAGGAATCTGATTCTCACCCCAGGAAGAAGAAGAAGGAAAAG
AAGGAGGACATTTTCCGCAGATTTCCAGTGGCCCCACTGATCCCTTATCCACTCATCACT
AAGGAGGATATAAATGCTATAGAAATGGAAGAAGACAAAAGAGACCTGATATCTCGAGAG
ATCAGCAAATTCAGAGACACACATAAGAAACTGGAAGAAGAGAAAGGCAAAAAGGAAAAA
GAAAGACAGGAAATTGAGAAAGAACGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGA
GAAAGGCGAGAACGGGAACGAGAAAGGGAAAGAGAACGTGAACGAGAAAAGGAGAAAGAA
CGGGAGCGGGAACGAGAACGGGATAGGGACCGTGACCGGACAAAAGAGAGAGACCGAGAT
CGGGATCGAGAGAGAGATCGTGACCGGGATAGAGAAAGGAGCTCAGATCGTAATAAGGAT
CGCAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGAGAGCGGGAAAGAGAG
AGAGAGAGAGAACGAGAGCGAGAACGAGAACGGGAGCGAGAGAGAGAGCGAGAGAGGGAA
CGGGAGCGAGAAAGAGAAAAAGACAAAAAACGGGACCGAGAAGAAGATGAAGAAGATGCA
TACGAACGAAGAAAACTTGAAAGAAAACTCCGAGAGAAAGAAGCTGCTTATCAAGAGCGC
CTTAAGAATTGGGAAATCAGAGAACGAAAGAAAACCCGGGAATATGAGAAAGAAGCTGAA
AGAGAAGAAGAAAGAAGAAGAGAAATGGCCAAAGAAGCTAAACGACTAAAAGAATTCTTA
GAAGACTATGATGATGATAGAGATGACCCCAAATATTACAGAGGAAGTGCTCTTCAGAAA
AGGTTGCGTGATAGAGAAAAGGAAATGGAAGCAGATGAACGAGATAGGAAGAGAGAGAAG
GAGGAGCTTGAGGAAATCAGGCAGCGCCTTCTGGCAGAAGGGCATCCAGATCCAGATGCA
GAGCTCCAGAGGATGGAACAAGAGGCTGAGAGGCGCAGGCAGCCACAAATAAAGCAAGAG
CCAGAATCAGAAGAGGAGGAAGAAGAAAAGCAAGAAAAAGAAGAAAAACGAGAAGAACCC
ATGGAAGAGGAAGAGGAGCCAGAGCAAAAGCCTTGTCTGAAACCTACTCTGAGGCCCATC
AGCTCTGCTCCATCTGTTTCCTCTGCCAGTGGCAATGCAACACCTAACACTCCTGGGGAT
GAGTCTCCCTGTGGTATTATTATTCCTCATGAAAACTCACCAGATCAACAGCAACCTGAG
GAGCATAGGCCAAAAATAGGACTAAGTCTTAAACTGGGTGCTTCCAATAGTCCTGGTCAG
CCTAATTCTGTGAAGAGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTTGAGGAT
GAAGACAGTGATGACGTACCCCGAAAAAGGAAACTGGTTCCCTTGGATTATGGTGAAGAT
GATAAAAATGCAACCAAAGGCACTGTAAACACTGAAGAAAAGCGTAAACACATTAAGAGT
CTCATTGAGAAAATCCCTACAGCCAAACCTGAGCTCTTCGCTTATCCCCTGGATTGGTCT
ATTGTGGATTCTATACTGATGGAACGTCGAATTAGACCATGGATTAATAAGAAAATCATA
GAATATATAGGTGAAGAAGAAGCTACATTAGTTGATTTTGTTTGTTCTAAGGTTATGGCT
CATAGTTCACCCCAGAGCATTTTAGATGATGTTGCCATGGTACTTGATGAAGAAGCAGAA
GTTTTTATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGT
CTTGTGAAG
Protein sequence predicted based on nucleotide sequence
MSFPPHLNRPPMGIPALPPGIPPPQFPGFPPPVPPGTPMIPVPMSIMAPAPTVLVPTVSM VGKHLGARKDHPGLKAKENDENCGPTTTVFVGNISEKASDMLIRQLLAKCGLVLSWKRVQ GASGKLQAFGFCEYKEPESTLRALRLLHDLQIGEKKLLVKVDAKTKAQLDEWKAKKKASN GNARPETVTNDDEEALDEETKRRDQMIKGAIEVLIREYSSELNAPSQESDSHPRKKKKEK KEDIFRRFPVAPLIPYPLITKEDINAIEMEEDKRDLISREISKFRDTHKKLEEEKGKKEK ERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRDRDRTKERDRD RDRERDRDRDRERSSDRNKDRSRSREKSRDRERERERERERERERERERERERERERERE REREREKDKKRDREEDEEDAYERRKLERKLREKEAAYQERLKNWEIRERKKTREYEKEAE REEERRREMAKEAKRLKEFLEDYDDDRDDPKYYRGSALQKRLRDREKEMEADERDRKREK EELEEIRQRLLAEGHPDPDAELQRMEQEAERRRQPQIKQEPESEEEEEEKQEKEEKREEP MEEEEEPEQKPCLKPTLRPISSAPSVSSASGNATPNTPGDESPCGIIIPHENSPDQQQPE EHRPKIGLSLKLGASNSPGQPNSVKRKKLPVDSVFNKFEDEDSDDVPRKRKLVPLDYGED DKNATKGTVNTEEKRKHIKSLIEKIPTAKPELFAYPLDWSIVDSILMERRIRPWINKKII EYIGEEEATLVDFVCSKVMAHSSPQSILDDVAMVLDEEAEVFIVKMWRLLIYETEAKKIG LVK
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
