Gene:
Gene Symbol:
ANKLE2
Gene Name:
ankyrin repeat and LEM domain containing 2
Original Clone ID:
ccsbBroadEn_07840
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC043157.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516353
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCTGTGGCCGCGGCTGGCGGCGGCCGAGTGGGCGGCGCTGGCCTGGGAGCTGCTGGGC
GCCTCGGTGCTGCTGATCGCTGTGCGGTGGCTGGTGCGGCGGCTGGGCCCGCGGCCGGGA
GGTCTGGGCCGCAGCGGGACCCCAGTTCCTCCGCCAAGCGCGGCCGCCGCCCCCGCCTCA
GGTGAAATGACAATGGATGCTCTGTTGGCTCGATTGAAACTTCTGAATCCAGATGACCTT
AGAGAAGAAATCGTCAAAGCCGGATTGAAATGTGGACCCATTACATCAACTACAAGGTTC
ATTTTTGAGAAAAAATTGGCTCAGGCTTTACTGGAGCAAGGAGGAAGGCTGTCTTCTTTC
TACTACCATGAGGCAGGTGTCACAGCTCTCAGCCAGGACCCACAAAGGATTTTGAAGCCA
GCTGAAGGGAACCCAACTGATCAGGCTGGTTTTTCTGAAGACAGAGATTTTGGTTACAGT
GTGGGCCTGAATCCTCCAGAGGAGGAAGCTGTGACATCCAAGACCTGCTCGGTGCCCCCT
AGTGACACCGACACCTACAGAGCTGGAGCGACTGCGTCTAAGGAGCCGCCCCTGTACTAT
GGGGTGTGTCCAGTGTATGAGGACGTCCCAGCGAGAAATGAAAGGATCTATGTTTATGAA
AATAAAAAGGAAGCATTGCAAGCTGTCAAGATGATCAAAGGGTCCCGATTTAAAGCTTTT
TCTACCAGAGAAGACGCTGAGAAATTTGCTAGAGGAATTTGTGATTATTTCCCTTCTCCA
AGCAAAACGTCCTTACAACTGTCTCCTGTGAAAACAGCTCCACTCTTTAGCAATGACAGG
TTGAAAGATGGTTTGTGCTTGTCGGAATCAGAAACAGTCAACAAAGAGCGAGCGAACAGT
TACAAAAATCCCCGCACGCAGGACCTCACCGCCAAGCTTCGGAAAGCTGTGGAGAAGGGA
GAGGAGGACACCTTTTCTGACCTTATCTGGAGCAACCCCCGGTATCTGATAGGCTCAGGA
GACAACCCCACTATCGTGCAGGAAGGGTGCAGGTACAACGTGATGCATGTTGCTGCCAAA
GAGAACCAGGCTTCCATCTGCCAGCTGACTCTGGACGTCCTGGAGAACCCTGACTTCATG
AGGCTGATGTACCCTGATGACGACGAGGCCATGCTGCAGAAGCGTATCCGTTACGTGGTG
GACCTGTACCTCAACACCCCCGACAAGATGGGCTATGACACACCGTTGCATTTTGCTTGT
AAGTTTGGAAATGCAGATGTAGTCAACGTGCTTTCGTCACACCATTTGATTGTAAAAAAC
TCAAGGAATAAATATGATAAAACACCTGAAGATGTAATTTGTGAAAGAAGCAAAAATAAA
TCTGTGGAACTGAAGGAGCGGATCAGAGAGTATTTAAAGGGCCACTACTACGTGCCCCTC
GTGAGAGCGGAAGAGACTTCTTCTCCAGTCATCGGGGAGCTGTGGTCCCCAGACCAGACG
GCTGAGGCCTCTCACGTCAGCCGCTATGGAGGCAGCCCCAGAGACCCGGTACTGACCCTG
AGAGCCTTCGCAGGGCCCCTGAGTCCAGCCAAGGCAGAAGATTTTCGCAAGCTCTGGAAA
ACTCCACCTCGAGAGAAAGCAGGCTTCCTTCACCACGTCAAGAAGTCGGACCCGGAAAGA
GGCTTTGAGAGAGTGGGAAGGGAGCTAGCTCATGAGCTGGGGTATCCCTGGGTTGAATAC
TGGGAATTTCTGGGCTGTTTTGTTGATCTGTCTTCCCAGGAAGGCCTGCAAAGACTAGAA
GAATATCTCACACAGCAGGAAATAGGCAAAAAGGCTCAACAAGAAACAGGAGAACGGGAA
GCCTCCTGCCGAGATAAAGCCACCACGTCTGGCAGCAATTCCATTTCCGTGAGGGCGTTT
CTAGATGAAGATGACATGAACTTGGAAGAAATAAAAAATCGGCAAAATGCAGCTCGAAAT
AACAGCCCGCCCACAGTCGGTGCTTTTGGACATACGAGGTGCAGCGCCTTCCCCTTGGAG
CAGGAGGCAGACCTCATAGAAGCCGCCGAGCCGGGAGGTCCACACAGCAGCAGAAATGGG
CTCTGCCATCCTCTGAATCACAGCAGGACCCTGGCGGGCAAGAGACCAAAGGCCCCCCAT
GGGGAGGAAGCCCATCTGCCACCTGTCTCGGATTTGACTGTTGAGTTTGATAAACTGAAT
TTGCAAAATATAGGACGTAGCGTTTCCAAGACACCAGATGAAAGTACAAAAACTAAAGAT
CAGATCCTGACTTCAAGAATCAATGCAGTAGAAAGAGACTTGTTAGAGCCTTCTCCCGCA
GACCAACTCGGGAATGGCCACAGGAGGACAGAAAGTGAAATGTCAGCCAGGATCGCTAAA
ATGTCCTTGAGTCCCAGCAGCCCCAGGCACGAGGATCAGCTCGAGGTCACCAGGGAACCG
GCCAGGCGGCTCTTCCTTTTTGGAGAGGAGCCATCAAAACTCGATCAGGATGTTTTGGCC
GCTCTTGAATGTGCAGACGTCGACCCCCATCAGTTCCCGGCCGTGCACAGATGGAAGAGT
GCTGTCCTGTGCTACTCACCCTCGGACAGACAGAGTTGGCCCAGTCCCGCGGTGAAAGGA
AGGTTCAAGTCTCAGCTGCCAGATCTCAGTGGCCCTCACAGCTACAGTCCGGGGAGAAAC
AGCGTGGCTGGAAGCAACCCCGCAAAGCCAGGCCTGGGCAGTCCTGGGCGCTACAGCCCC
GTGCACGGGAGCCAGCTCCGCAGGATGGCGCGCCTGGCTGAGCTTGCCGCCCTG
Protein sequence predicted based on nucleotide sequence
MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPAS GEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSF YYHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPP SDTDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAF STREDAEKFARGICDYFPSPSKTSLQLSPVKTAPLFSNDRLKDGLCLSESETVNKERANS YKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAK ENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFAC KFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPL VRAEETSSPVIGELWSPDQTAEASHVSRYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWK TPPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLE EYLTQQEIGKKAQQETGEREASCRDKATTSGSNSISVRAFLDEDDMNLEEIKNRQNAARN NSPPTVGAFGHTRCSAFPLEQEADLIEAAEPGGPHSSRNGLCHPLNHSRTLAGKRPKAPH GEEAHLPPVSDLTVEFDKLNLQNIGRSVSKTPDESTKTKDQILTSRINAVERDLLEPSPA DQLGNGHRRTESEMSARIAKMSLSPSSPRHEDQLEVTREPARRLFLFGEEPSKLDQDVLA ALECADVDPHQFPAVHRWKSAVLCYSPSDRQSWPSPAVKGRFKSQLPDLSGPHSYSPGRN SVAGSNPAKPGLGSPGRYSPVHGSQLRRMARLAELAAL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
