6-Mar (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MARCH6
Gene Name:  membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase
Original Clone ID:
ccsbBroadEn_07583
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC142679.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516374
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2730nts
Open reading frame: 1 to 2730

ATGGACACCGCGGAGGAAGACATATGTAGAGTGTGTCGGTCAGAAGGAACACCTGAGAAA
CCGCTTTATCATCCTTGTGTATGTACTGGCAGTATTAAGTTTATCCATCAAGAATGCTTA
GTTCAATGGCTGAAACACAGTCGAAAAGAATACTGTGAATTATGCAAGCACAGATTTGCT
TTTACACCAATTTATTCTCCAGATATGCCTTCACGGCTTCCAATTCAAGACATATTTGCT
GGACTGGTTACAAGTATTGGCACTGCAATACGATATTGGTTTCATTATACACTTGTGGCC
TTTGCATGGTTGGGAGTTGTTCCTCTTACAGCATGCCGCATCTACAAGTGCTTGTTTACT
GGCTCCGTGAGCTCACTACTGACGCTGCCATTAGATATGCTGTCAACGGAAAATTTGTTG
GCAGATTGTTTGCAGGGTTGTTTTGTGGTGACGTGCACACTGTGTGCATTCATCAGCCTG
GTGTGGTTGAGAGAGCAGATAGTCCATGGGGGAGCACCAATTTGGTTGGAGCATGCTGCC
CCACCGTTCAATGCTGCGGGGCATCACCAAAATGAGGCTCCAGCAGGAGGAAATGGTGCA
GAAAATGTTGCTGCTGATCAGCCTGCTAACCCACCAGCTGAGAACGCAGTGGTGGGGGAA
AACCCTGATGCCCAGGATGACCAGGCAGAAGAGGAGGAGGAGGACAATGAGGAGGAAGAT
GACGCTGGTGTGGAGGATGCGGCAGATGCTAATAACGGAGCCCAGGATGACATGAATTGG
AATGCTTTAGAATGGGACCGAGCTGCTGAAGAGCTTACATGGGAAAGAATGCTAGGACTT
GATGGATCACTAGTTTTTCTGGAACATGTCTTCTGGGTGGTATCTTTAAATACACTGTTC
ATTCTTGTTTTTGCATTTTGCCCTTACCATATTGGTCATTTCTCCCTTGTTGGTTTGGGA
TTTGAAGAACACGTCCAAGCATCTCATTTTGAAGGCCTAATCACAACCATAGTTGGGTAT
ATACTTTTAGCAATAACACTGATAATTTGTCATGGCTTGGCAACTCTTGTGAAATTTCAT
AGATCTCGTCGCTTACTGGGAGTCTGCTATATTGTTGTTAAGGTCTCTTTGTTAGTGGTG
GTAGAAATTGGAGTATTCCCTCTCATTTGTGGTTGGTGGCTGGATATCTGTTCCTTGGAA
ATGTTTGATGCTACTCTGAAAGATCGAGAACTGAGCTTTCAGTCGGCTCCAGGTACTACC
ATGTTTCTGCATTGGCTAGTGGGAATGGTATATGTCTTCTACTTTGCCTCCTTCATTCTA
CTACTGAGAGAGGTACTTCGACCTGGTGTCCTGTGGTTTCTAAGGAATTTGAATGATCCA
GATTTCAATCCAGTACAGGAAATGATCCATTTGCCAATATATAGGCATCTCCGAAGATTT
ATTTTGTCAGTGATTGTCTTTGGCTCCATTGTCCTCCTGATGCTTTGGCTTCCTATACGT
ATAATTAAGAGTGTGCTGCCTAATTTTCTTCCATACAATGTCATGCTCTACAGTGATGCT
CCAGTGAGTGAACTGTCCCTCGAGCTGCTTCTGCTTCAGGTTGTCTTGCCAGCATTACTC
GAACAGGGACACACGAGGCAGTGGCTGAAGGGGCTGGTGCGAGCGTGGACTGTGACCGCC
GGATACTTGCTGGATCTTCATTCTTATTTATTGGGAGACCAGGAAGAAAATGAAAACAGT
GCAAATCAACAAGTTAACAATAATCAGCATGCTCGAAATAACAACGCTATTCCTGTGGTG
GGAGAAGGCCTTCATGCAGCCCACCAAGCCATACTCCAGCAGGGAGGGCCTGTTGGCTTT
CAGCCTTACCGCCGACCTTTAAATTTTCCACTCAGGATATTTCTGTTGATTGTCTTCATG
TGTATAACATTACTGATTGCCAGCCTCATCTGCCTTACTTTACCAGTATTTGCTGGCCGT
TGGTTAATGTCGTTTTGGACGGGGACTGCCAAAATCCATGAGCTCTACACAGCTGCTTGT
GGTCTCTATGTTTGCTGGCTAACCATAAGGGCTGTGACGGTGATGGTGGCATGGATGCCT
CAGGGACGCAGAGTGATCTTCCAGAAGGTTAAAGAGTGGTCTCTCATGATCATGAAGACT
TTGATAGTTGCGGTGCTGTTGGCTGGAGTTGTCCCTCTCCTTCTGGGGCTCCTGTTTGAG
CTGGTCATTGTGGCTCCCCTGAGGGTTCCCTTGGATCAGACTCCTCTTTTTTATCCATGG
CAGGACTGGGCACTTGGAGTCCTGCATGCCAAAATCATTGCAGCTATAACATTGATGGGT
CCTCAGTGGTGGTTGAAAACTGTAATTGAACAGGTTTACGCAAATGGCATCCGGAACATT
GACCTTCACTATATTGTTCGTAAACTGGCAGCTCCCGTGATCTCTGTGCTGTTGCTTTCC
CTGTGTGTACCTTATGTCATAGCTTCTGGTGTTGTTCCTTTACTAGGTGTTACTGCGGAA
ATGCAAAACTTAGTCCATCGGCGGATTTATCCATTTTTACTGATGGTCGTGGTATTGATG
GCAATTTTGTCCTTCCAAGTCCGCCAGTTTAAGCGCCTTTATGAACATATTAAAAATGAC
AAGTACCTTGTGGGTCAACGACTCGTGAACTACGAACGGAAATCTGGCAAACAAGGCTCA
TCTCCACCGCCTCCACAGTCATCCCAAGAA

Protein sequence predicted based on nucleotide sequence

MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFA
FTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFT
GSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAA
PPFNAAGHHQNEAPAGGNGAENVAADQPANPPAENAVVGENPDAQDDQAEEEEEDNEEED
DAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLF
ILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFH
RSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTT
MFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRF
ILSVIVFGSIVLLMLWLPIRIIKSVLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALL
EQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVV
GEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGR
WLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKT
LIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMG
PQWWLKTVIEQVYANGIRNIDLHYIVRKLAAPVISVLLLSLCVPYVIASGVVPLLGVTAE
MQNLVHRRIYPFLLMVVVLMAILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGS
SPPPPQSSQE

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable