DNASU Plasmid | NSMAF (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NSMAF

Gene Name: neutral sphingomyelinase (N-SMase) activation associated factor

Original Clone ID:

ccsbBroadEn_07239

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC041124.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516376

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2752nts

Open reading frame: 1 to 2752

Reference Sequence Alignment

ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTGCAGCTCTACTCCAAGGAGAGA
TTTTCCTTGCTGCTGCTTAACTTGGAGGAGTACTACTTTGAACAGCATAGAGCCAATCAC
ATTTTGCACAAGGGCAGTCACCATGAAAGGAAAATCAGAGGCTCCTTAAAAATATGTTCA
AAATCGGTGATTTTTGAACCAGATTCAATATCCCAGCCCATCATCAAGATTCCTTTGAGA
GACTGTATAAAAATAGGAAAGCATGGAGAAAATGGAGCCAATAGACACTTCACAAAGGCA
AAATCTGGGGGTATTTCACTCATTTTCAGTCAGGTATATTTCATTAAAGAACATAATGTT
GTTGCACCATATAAAATAGAAAGGGGCAAAATGGAATATGTTTTTGAATTGGATGTTCCC
GGGAAAGTGGAAGATGTTGTGGAGACGTTGCTTCAGCTTCACAGAGCATCCTGCCTTGAC
AAATTGGGTGACCAAACCGCCATGATAACAGCTATTTTGCAGTCTCGTTTAGCTAGAACA
TCATTTGACAAAAACAGGTTCCAAAACATTTCTGAAAAGCTGCACATGGAATGCAAAGCA
GAAATGGTGACGCCTCTGGTGACTAATCCTGGACACGTGTGCATCACGGACACAAACCTG
TATTTTCAGCCCCTCAACGGCTACCCGAAACCTGTGGTCCAGATAACACTCCAAGATGTC
CGCCGCATCTACAAAAGGAGGCACGGCCTCATGCCTCTGGGCTTGGAAGTATTTTGCACA
GAAGATGATCTGTGTTCCGACATCTACCTAAAGTTCTATGAACCTCAAGATAGAGATGAT
CTCTATTTTTACATTGCCACATACCTAGAGCACCATGTGGCGGAGCACACTGCTGAGAGC
TACATGCTGCAGTGGCAGCGTGGACACCTTTCCAACTATCAGTACCTCCTTCACCTCAAC
AACCTGGCCGACCGCAGCTGCAACGACCTCTCCCAGTACCCTGTGTTTCCATGGATAATA
CATGATTATTCCAGCTCAGAACTAGATTTGTCAAATCCAGGAACCTTCCGGGATCTCAGT
AAGCCAGTAGGGGCCCTAAATAAGGAACGGCTGGAGAGACTACTGACACGCTACCAGGAA
ATGCCTGAACCAAAGTTCATGTATGGGAGTCACTACTCTTCCCCGGGTTATGTACTTTTT
TATCTTGTTAGGATTGCACCAGAGTATATGCTGTGCCTGCAGAATGGAAGATTTGATAAT
GCAGATAGAATGTTCAACAGTATTGCAGAAACTTGGAAAAACTGTCTGGATGGTGCAACG
GATTTTAAAGAGTTAATTCCAGAATTCTATGGTGATGATGTGAGCTTTCTAGTCAATAGC
CTGAAGTTGGATTTGGGAAAGAGACAAGGAGGACAGATGGTTGACGATGTGGAGCTTCCC
CCTTGGGCTTCCAGTCCCGAGGACTTTCTCCAGAAGAGCAAAGATGCATTGGAAAGCAAT
TATGTGTCTGAACACCTTCACGAGTGGATTGATCTAATATTTGGCTACAAACAAAAAGGG
AGTGATGCAGTTGGGGCCCATAATGTATTTCATCCCCTGACCTATGAAGGAGGTGTAGAC
TTGAACAGCATCCAGGATCCTGATGAGAAGGTAGCCATGCTTACGCAAATCTTGGAATTT
GGGCAGACACCAAAACAACTATTTGTGACACCACATCCTCGAAGGATCACCCCAAAGTTT
AAAAGTTTGTCCCAGACCTCCAGTTATAATGCTTCTATGGCAGATTCCCCAGGTGAAGAG
TCTTTTGAAGACCTGACCGAAGAAAGCAAAACACTGGCCTGGAATAACATCACCAAACTG
CAGTTACACGAGCACTATAAAATCCACAAAGAAGCAGTTACTGGAATCACGGTCTCTCGC
AATGGATCTTCAGTATTCACAACATCCCAAGATTCCACCTTGAAGATGTTTTCTAAAGAA
TCAAAAATGCTACAAAGAAGTATATCATTTTCAAATATGGCTTTATCGTCTTGTTTACTT
TTACCAGGAGATGCCACTGTCATAACTTCTTCATGGGATAATAATGTCTATTTTTATTCC
ATAGCATTTGGAAGACGCCAGGACACGTTAATGGGACATGATGATGCTGTTAGTAAGATC
TGTTGGCATGACAACAGGCTATATTCTGCATCGTGGGACTCTACAGTGAAGGTGTGGTCT
GGTGTTCCTGCAGAGATGCCAGGCACCAAAAGACACCACTTTGACTTGCTGGCCGAGCTG
GAACATGATGTCAGTGTAGATACAATCAGTTTAAATGCTGCAAGCACACTGTTAGTTTCC
GGCACCAAAGAAGGCACAGTGAATATTTGGGACCTCACAACGGCCACCTTAATGCACCAG
ATTCCATGCCATTCAGGGATTGTATGTGACACTGCTTTTAGCCCAGATAGTCGCCATGTC
CTCAGCACAGGAACAGATGGCTGTCTTAATGTCATTGATGTGCAGACAGGAATGCTCATC
TCCTCCATGACATCAGATGAGCCCCAGAGGTGCTTTGTCTGGGATGGAAATTCCGTTTTA
TCTGGCAGTCAGTCTGGTGAACTGCTCGTTTGGGACCTCCTTGGAGCAAAAATCAGTGAG
AGAATACAGGGCCACACAGGTGCTGTGACATGTATATGGATGAATGAACAGTGTAGCAGT
ATCATCACAGGAGGGGAAGACAGACAAATTATATTCTGGAAATTGCAGTAT

Protein sequence predicted based on nucleotide sequence

MAFIRKKQQEQQLQLYSKERFSLLLLNLEEYYFEQHRANHILHKGSHHERKIRGSLKICS
KSVIFEPDSISQPIIKIPLRDCIKIGKHGENGANRHFTKAKSGGISLIFSQVYFIKEHNV
VAPYKIERGKMEYVFELDVPGKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLART
SFDKNRFQNISEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDV
RRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAES
YMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIIHDYSSSELDLSNPGTFRDLS
KPVGALNKERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDN
ADRMFNSIAETWKNCLDGATDFKELIPEFYGDDVSFLVNSLKLDLGKRQGGQMVDDVELP
PWASSPEDFLQKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVD
LNSIQDPDEKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGEE
SFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTTSQDSTLKMFSKE
SKMLQRSISFSNMALSSCLLLPGDATVITSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKI
CWHDNRLYSASWDSTVKVWSGVPAEMPGTKRHHFDLLAELEHDVSVDTISLNAASTLLVS
GTKEGTVNIWDLTTATLMHQIPCHSGIVCDTAFSPDSRHVLSTGTDGCLNVIDVQTGMLI
SSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKISERIQGHTGAVTCIWMNEQCSS
IITGGEDRQIIFWKLQY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NSMAF

Symbol Nomenclature :

NSMAF

SYNONYM :

FAN

Designation :

factor associated with N-SMase activation|factor associated with neutral sphingomyelinase activation|protein FAN

Full Nomenclature :

neutral sphingomyelinase (N-SMase) activation associated factor

GENEID :

8439

HGNC :

8017

MIM :

603043

Vega :

OTTHUMG00000164352

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516376        ------------------------------------------------------------
NM_001144772.1      ATGACGGGCCAGGCCAGATTCCACCGCGGAGCCGAGGCATGCCGAGCTATCCGGCAGTGC
NM_003580.3         ------------------------------------------------------------
                                                                                

HsCD00516376        ----------------ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTG-----
NM_001144772.1      AGGTCCCGGCGGCCGCGGGCAGCTCACCCGGAAGGCCGCGCGGGGTCCGGGCGTGGATCC
NM_003580.3         ----------------ATGGCGTTTATCCGGAAGAAGCAGCAGGAGCAGCAGCTG-----
                                    . * .* * * *******..  .**.**. *.* .  **     

HsCD00516376        ------------CAGCTCTACTCCAAGGAGAGATTTTCCTTGCTGCTGCTTAACTTGGAG
NM_001144772.1      CGGCACGCGAGCCCGGGCGTTCGCAGGGGAGGATTTTCCTTGCTGCTGCTTAACTTGGAG
NM_003580.3         ------------CAGCTCTACTCCAAGGAGAGATTTTCCTTGCTGCTGCTTAACTTGGAG
                                *.*  * :   **.**...*****************************

HsCD00516376        GAGTACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAA
NM_001144772.1      GAGTACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAA
NM_003580.3         GAGTACTACTTTGAACAGCATAGAGCCAATCACATTTTGCACAAGGGCAGTCACCATGAA
                    ************************************************************

HsCD00516376        AGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCA
NM_001144772.1      AGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCA
NM_003580.3         AGGAAAATCAGAGGCTCCTTAAAAATATGTTCAAAATCGGTGATTTTTGAACCAGATTCA
                    ************************************************************

HsCD00516376        ATATCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGA
NM_001144772.1      ATATCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGA
NM_003580.3         ATATCCCAGCCCATCATCAAGATTCCTTTGAGAGACTGTATAAAAATAGGAAAGCATGGA
                    ************************************************************

HsCD00516376        GAAAATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTC
NM_001144772.1      GAAAATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTC
NM_003580.3         GAAAATGGAGCCAATAGACACTTCACAAAGGCAAAATCTGGGGGTATTTCACTCATTTTC
                    ************************************************************

HsCD00516376        AGTCAGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGC
NM_001144772.1      AGTCAGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGC
NM_003580.3         AGTCAGGTATATTTCATTAAAGAACATAATGTTGTTGCACCATATAAAATAGAAAGGGGC
                    ************************************************************

HsCD00516376        AAAATGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACG
NM_001144772.1      AAAATGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACG
NM_003580.3         AAAATGGAATATGTTTTTGAATTGGATGTTCCCGGGAAAGTGGAAGATGTTGTGGAGACG
                    ************************************************************

HsCD00516376        TTGCTTCAGCTTCACAGAGCATCCTGCCTTGACAAATTGGGTGACCAAACCGCCATGATA
NM_001144772.1      TTGCTTCAGCTTCACAGAGCATCCTGCCTTGACAAATTGGGTGACCAAACCGCCATGATA
NM_003580.3         TTGCTTCAGCTTCACAGAGCATCCTGCCTTGACAAATTGGGTGACCAAACCGCCATGATA
                    ************************************************************

HsCD00516376        ACAGCTATTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGGTTCCAAAAC
NM_001144772.1      ACAGCTATTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGGTTCCAAAAC
NM_003580.3         ACAGCTATTTTGCAGTCTCGTTTAGCTAGAACATCATTTGACAAAAACAGGTTCCAAAAC
                    ************************************************************

HsCD00516376        ATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGGTGACGCCTCTGGTGACTAAT
NM_001144772.1      ATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGGTGACGCCTCTGGTGACTAAT
NM_003580.3         ATTTCTGAAAAGCTGCACATGGAATGCAAAGCAGAAATGGTGACGCCTCTGGTGACTAAT
                    ************************************************************

HsCD00516376        CCTGGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAACGGCTACCCG
NM_001144772.1      CCTGGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAACGGCTACCCG
NM_003580.3         CCTGGACACGTGTGCATCACGGACACAAACCTGTATTTTCAGCCCCTCAACGGCTACCCG
                    ************************************************************

HsCD00516376        AAACCTGTGGTCCAGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGC
NM_001144772.1      AAACCTGTGGTCCAGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGC
NM_003580.3         AAACCTGTGGTCCAGATAACACTCCAAGATGTCCGCCGCATCTACAAAAGGAGGCACGGC
                    ************************************************************

HsCD00516376        CTCATGCCTCTGGGCTTGGAAGTATTTTGCACAGAAGATGATCTGTGTTCCGACATCTAC
NM_001144772.1      CTCATGCCTCTGGGCTTGGAAGTATTTTGCACAGAAGATGATCTGTGTTCCGACATCTAC
NM_003580.3         CTCATGCCTCTGGGCTTGGAAGTATTTTGCACAGAAGATGATCTGTGTTCCGACATCTAC
                    ************************************************************

HsCD00516376        CTAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTA
NM_001144772.1      CTAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTA
NM_003580.3         CTAAAGTTCTATGAACCTCAAGATAGAGATGATCTCTATTTTTACATTGCCACATACCTA
                    ************************************************************

HsCD00516376        GAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACAC
NM_001144772.1      GAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACAC
NM_003580.3         GAGCACCATGTGGCGGAGCACACTGCTGAGAGCTACATGCTGCAGTGGCAGCGTGGACAC
                    ************************************************************

HsCD00516376        CTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGAC
NM_001144772.1      CTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGAC
NM_003580.3         CTTTCCAACTATCAGTACCTCCTTCACCTCAACAACCTGGCCGACCGCAGCTGCAACGAC
                    ************************************************************

HsCD00516376        CTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGAT
NM_001144772.1      CTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGAT
NM_003580.3         CTCTCCCAGTACCCTGTGTTTCCATGGATAATACATGATTATTCCAGCTCAGAACTAGAT
                    ************************************************************

HsCD00516376        TTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAA
NM_001144772.1      TTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAA
NM_003580.3         TTGTCAAATCCAGGAACCTTCCGGGATCTCAGTAAGCCAGTAGGGGCCCTAAATAAGGAA
                    ************************************************************

HsCD00516376        CGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG
NM_001144772.1      CGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG
NM_003580.3         CGGCTGGAGAGACTACTGACACGCTACCAGGAAATGCCTGAACCAAAGTTCATGTATGGG
                    ************************************************************

HsCD00516376        AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTAT
NM_001144772.1      AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTAT
NM_003580.3         AGTCACTACTCTTCCCCGGGTTATGTACTTTTTTATCTTGTTAGGATTGCACCAGAGTAT
                    ************************************************************

HsCD00516376        ATGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCA
NM_001144772.1      ATGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCA
NM_003580.3         ATGCTGTGCCTGCAGAATGGAAGATTTGATAATGCAGATAGAATGTTCAACAGTATTGCA
                    ************************************************************

HsCD00516376        GAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTC
NM_001144772.1      GAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTC
NM_003580.3         GAAACTTGGAAAAACTGTCTGGATGGTGCAACGGATTTTAAAGAGTTAATTCCAGAATTC
                    ************************************************************

HsCD00516376        TATGGTGATGATGTGAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAA
NM_001144772.1      TATGGTGATGATGTGAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAA
NM_003580.3         TATGGTGATGATGTGAGCTTTCTAGTCAATAGCCTGAAGTTGGATTTGGGAAAGAGACAA
                    ************************************************************

HsCD00516376        GGAGGACAGATGGTTGACGATGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTT
NM_001144772.1      GGAGGACAGATGGTTGACGACGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTT
NM_003580.3         GGAGGACAGATGGTTGACGACGTGGAGCTTCCCCCTTGGGCTTCCAGTCCCGAGGACTTT
                    ******************** ***************************************

HsCD00516376        CTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGG
NM_001144772.1      CTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGG
NM_003580.3         CTCCAGAAGAGCAAAGATGCATTGGAAAGCAATTATGTGTCTGAACACCTTCACGAGTGG
                    ************************************************************

HsCD00516376        ATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA
NM_001144772.1      ATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA
NM_003580.3         ATTGATCTAATATTTGGCTACAAACAAAAAGGGAGTGATGCAGTTGGGGCCCATAATGTA
                    ************************************************************

HsCD00516376        TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAG
NM_001144772.1      TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAG
NM_003580.3         TTTCATCCCCTGACCTATGAAGGAGGTGTAGACTTGAACAGCATCCAGGATCCTGATGAG
                    ************************************************************

HsCD00516376        AAGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTG
NM_001144772.1      AAGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTG
NM_003580.3         AAGGTAGCCATGCTTACGCAAATCTTGGAATTTGGGCAGACACCAAAACAACTATTTGTG
                    ************************************************************

HsCD00516376        ACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGACCTCCAGTTAT
NM_001144772.1      ACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGACCTCCAGTTAT
NM_003580.3         ACACCACATCCTCGAAGGATCACCCCAAAGTTTAAAAGTTTGTCCCAGACCTCCAGTTAT
                    ************************************************************

HsCD00516376        AATGCTTCTATGGCAGATTCCCCAGGTGAAGAGTCTTTTGAAGACCTGACCGAAGAAAGC
NM_001144772.1      AATGCTTCTATGGCAGATTCCCCAGGTGAAGAGTCTTTTGAAGACCTGACCGAAGAAAGC
NM_003580.3         AATGCTTCTATGGCAGATTCCCCAGGTGAAGAGTCTTTTGAAGACCTGACCGAAGAAAGC
                    ************************************************************

HsCD00516376        AAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTACACGAGCACTATAAAATCCAC
NM_001144772.1      AAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTACACGAGCACTATAAAATCCAC
NM_003580.3         AAAACACTGGCCTGGAATAACATCACCAAACTGCAGTTACACGAGCACTATAAAATCCAC
                    ************************************************************

HsCD00516376        AAAGAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATTCACAACATCC
NM_001144772.1      AAAGAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATTCACAACATCC
NM_003580.3         AAAGAAGCAGTTACTGGAATCACGGTCTCTCGCAATGGATCTTCAGTATTCACAACATCC
                    ************************************************************

HsCD00516376        CAAGATTCCACCTTGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCA
NM_001144772.1      CAAGATTCCACCTTGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCA
NM_003580.3         CAAGATTCCACCTTGAAGATGTTTTCTAAAGAATCAAAAATGCTACAAAGAAGTATATCA
                    ************************************************************

HsCD00516376        TTTTCAAATATGGCTTTATCGTCTTGTTTACTTTTACCAGGAGATGCCACTGTCATAACT
NM_001144772.1      TTTTCAAATATGGCTTTATCGTCTTGTTTACTTTTACCAGGAGATGCCACTGTCATAACT
NM_003580.3         TTTTCAAATATGGCTTTATCGTCTTGTTTACTTTTACCAGGAGATGCCACTGTCATAACT
                    ************************************************************

HsCD00516376        TCTTCATGGGATAATAATGTCTATTTTTATTCCATAGCATTTGGAAGACGCCAGGACACG
NM_001144772.1      TCTTCATGGGATAATAATGTCTATTTTTATTCCATAGCATTTGGAAGACGCCAGGACACG
NM_003580.3         TCTTCATGGGATAATAATGTCTATTTTTATTCCATAGCATTTGGAAGACGCCAGGACACG
                    ************************************************************

HsCD00516376        TTAATGGGACATGATGATGCTGTTAGTAAGATCTGTTGGCATGACAACAGGCTATATTCT
NM_001144772.1      TTAATGGGACATGATGATGCTGTTAGTAAGATCTGTTGGCATGACAACAGGCTATATTCT
NM_003580.3         TTAATGGGACATGATGATGCTGTTAGTAAGATCTGTTGGCATGACAACAGGCTATATTCT
                    ************************************************************

HsCD00516376        GCATCGTGGGACTCTACAGTGAAGGTGTGGTCTGGTGTTCCTGCAGAGATGCCAGGCACC
NM_001144772.1      GCATCGTGGGACTCTACAGTGAAGGTGTGGTCTGGTGTTCCTGCAGAGATGCCAGGCACC
NM_003580.3         GCATCGTGGGACTCTACAGTGAAGGTGTGGTCTGGTGTTCCTGCAGAGATGCCAGGCACC
                    ************************************************************

HsCD00516376        AAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATGATGTCAGTGTAGATACAATC
NM_001144772.1      AAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATGATGTCAGTGTAGATACAATC
NM_003580.3         AAAAGACACCACTTTGACTTGCTGGCCGAGCTGGAACATGATGTCAGTGTAGATACAATC
                    ************************************************************

HsCD00516376        AGTTTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCACAGTGAATATT
NM_001144772.1      AGTTTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCACAGTGAATATT
NM_003580.3         AGTTTAAATGCTGCAAGCACACTGTTAGTTTCCGGCACCAAAGAAGGCACAGTGAATATT
                    ************************************************************

HsCD00516376        TGGGACCTCACAACGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGT
NM_001144772.1      TGGGACCTCACAACGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGT
NM_003580.3         TGGGACCTCACAACGGCCACCTTAATGCACCAGATTCCATGCCATTCAGGGATTGTATGT
                    ************************************************************

HsCD00516376        GACACTGCTTTTAGCCCAGATAGTCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTT
NM_001144772.1      GACACTGCTTTTAGCCCAGATAGTCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTT
NM_003580.3         GACACTGCTTTTAGCCCAGATAGTCGCCATGTCCTCAGCACAGGAACAGATGGCTGTCTT
                    ************************************************************

HsCD00516376        AATGTCATTGATGTGCAGACAGGAATGCTCATCTCCTCCATGACATCAGATGAGCCCCAG
NM_001144772.1      AATGTCATTGATGTGCAGACAGGAATGCTCATCTCCTCCATGACATCAGATGAGCCCCAG
NM_003580.3         AATGTCATTGATGTGCAGACAGGAATGCTCATCTCCTCCATGACATCAGATGAGCCCCAG
                    ************************************************************

HsCD00516376        AGGTGCTTTGTCTGGGATGGAAATTCCGTTTTATCTGGCAGTCAGTCTGGTGAACTGCTC
NM_001144772.1      AGGTGCTTTGTCTGGGATGGAAATTCCGTTTTATCTGGCAGTCAGTCTGGTGAACTGCTC
NM_003580.3         AGGTGCTTTGTCTGGGATGGAAATTCCGTTTTATCTGGCAGTCAGTCTGGTGAACTGCTC
                    ************************************************************

HsCD00516376        GTTTGGGACCTCCTTGGAGCAAAAATCAGTGAGAGAATACAGGGCCACACAGGTGCTGTG
NM_001144772.1      GTTTGGGACCTCCTTGGAGCAAAAATCAGTGAGAGAATACAGGGCCACACAGGTGCTGTG
NM_003580.3         GTTTGGGACCTCCTTGGAGCAAAAATCAGTGAGAGAATACAGGGCCACACAGGTGCTGTG
                    ************************************************************

HsCD00516376        ACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGACAGACAA
NM_001144772.1      ACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGACAGACAA
NM_003580.3         ACATGTATATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGACAGACAA
                    ************************************************************

HsCD00516376        ATTATATTCTGGAAATTGCAGTAT---
NM_001144772.1      ATTATATTCTGGAAATTGCAGTATTAA
NM_003580.3         ATTATATTCTGGAAATTGCAGTATTAA
                    ************************