DNASU Plasmid | PCDHA9 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PCDHA7

Gene Name: protocadherin alpha 7

Original Clone ID:

ccsbBroadEn_02234

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC104804.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516383

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2850nts

Open reading frame: 1 to 2850

Reference Sequence Alignment

ATGTTATACTCAAGTCGAGGAGATCCAGAGGGTCAGCCTCTACTGCTCTCGCTTCTGATC
CTCGCAATGTGGGTGGTGGGGAGCGGCCAGCTCCACTACTCCGTCCCGGAGGAAGCCGAA
CACGGCACCTTCGTGGGCCGCATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTG
CCGCGCCTGTTCCAGTTGGATTCCAAAGGCCGCGGGGACCTTCTGGAGGTAAATCTGCAG
AATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGGAGCTGTGCGGGCGGAGCGCG
GAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTTCCATGTGGAC
GTGGAGGTGAAGGACATTAACGACAACCCTCCAGTGTTCCCAGCGACACAAAAGAATCTG
TTCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCGTCCGATGCA
GATATCGGGGAGAACGCCCTGCTCACTTACAGACTGAGCCCCAATGAGTATTTCTTCCTG
GACGTGCCAACCAGCAACCAGCAGGTAAAACCTCTTGGACTTGTATTACGGAAACTTTTA
GACAGAGAAGAAACTCCGGAGCTTCATTTATTGCTCACGGCCACCGATGGAGGCAAACCC
GAGCTGACTGGCACCGTTCAATTACTCATCACGGTACTGGACAACAATGACAATGCCCCA
GTGTTCGACAGAACCCTGTATACGGTGAAATTACCAGAAAACGTTTCTATCGGAACGCTG
GTGATTCACCCCAATGCCTCAGATTTAGACGAAGGCTTGAATGGGGATATTATTTACTCC
TTCTCCAGTGATGTTTCTCCAGATATAAAATCCAAGTTCCACATGGACCCCTTAAGTGGG
GCAATCACAGTGATAGGACATATGGATTTTGAAGAAAGTAGAGCACACAAGATCCCAGTC
GAGGCTGTCGATAAAGGCTTCCCACCCCTGGCTGGTCATTGTACACTTCTTGTGGAAGTT
GTGGATGTAAATGACAATGCTCCACAGTTGACTATCAAAACGCTCTCGGTTCCTGTAAAA
GAGGACGCACAACTGGGGACAGTTATTGCCCTGATTAGTGTGATCGACCTAGACGCAGAT
GCCAACGGGCAGGTTACCTGCTCCCTGACGCCCCACGTCCCCTTCAAGCTGGTGTCCACC
TACAAGAATTACTACTCGTTGGTGCTGGACAGAGCTCTGGACCGCGAGAGTGTGTCCGCC
TACGAGCTGGTGGTTACCGCGCGGGACGGGGGCTCGCCTTCACTGTGGGCCACGGCCAGG
GTGTCTGTGGAGGTGGCCGACGTGAACGACAACGCACCAGCGTTCGCGCAGTCCGAGTAC
ACGGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGG
GACGCTGACGCGCAGGAGAACGCCCTGGTGTCCTACTCGCTGGTGGAGCGGCGGTTGGGC
GAGCGCTCGCTGTCGAGCTACGTGTCAGTGCACGCGGAGAGCGGCAAGGTGTACGCGCTG
CAGCCGTTGGACCACGAGGAGCTGGAGCTGCTACAGTTCCAGGTGAGCGCGCGCGACGCG
GGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
AATGCGCCGGCGCTGCTGACACCTCGGATGAGGGGCACTGACGGCGCAGTGAGCGAGATG
GTGCTGCGGTCGGTGGGCGCCGGCGTAGTGGTGGGGAAGGTGCGCGCAGTGGACGCCGAC
TCGGGCTACAACGCGTGGCTTTCATACGAGCTGCAGCCAGAAACGGCCAGCGCGAGCATC
CCGTTCCGCGTGGGGCTGTACACGGGCGAGATCAGCACAACGCGTGCCCTGGACGAAACG
GACGCACCGCGCCAGCGCCTACTGGTGCTGGTGAAAGACCACGGGGAGCCAGCGCTGACG
GCCACGGCCACTGTGCTGGTGTCGCTGGTGGAGAGCGGCCAGGCGCCAAAGTCATCGTCG
CGGGCGTCAGTGGGTGCCACGGGCCCCGAGGTGACGCTGGTGGATGTCAACGTGTACCTG
ATCATCGCCATCTGCGCGGTGTCTAGCCTGTTGGTTCTCACGCTGCTGCTGTACACTGTG
CTGCGGTGCTCGGCGATGCCCACCGAGGGCGAGTGCGCGCCTGGCAAGCCGACGCTGGTG
TGTTCTAGCGCGGTGGGGAGTTGGTCGTACTCGCAGCAGAGGAGGCAGAGGGTGTGCTCT
GGCGAGGGTAAGCAGAAGACCGACCTCATGGCCTTCAGCCCGGGCCTTTCTCCTTGTGCT
GGATCTACAGAGCGAACGGGAGAACCCTCTGCTTCCTCAGATTCAACTGGGAAGCCACGA
CAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTG
CACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCA
ACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCG
GGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGT
CCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGG
CAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAG
GAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAA
GGGAACAGCACGACTGACAACAGTGACCAG

Protein sequence predicted based on nucleotide sequence

MLYSSRGDPEGQPLLLSLLILAMWVVGSGQLHYSVPEEAEHGTFVGRIAQDLGLELAELV
PRLFQLDSKGRGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVD
VEVKDINDNPPVFPATQKNLFIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFFL
DVPTSNQQVKPLGLVLRKLLDREETPELHLLLTATDGGKPELTGTVQLLITVLDNNDNAP
VFDRTLYTVKLPENVSIGTLVIHPNASDLDEGLNGDIIYSFSSDVSPDIKSKFHMDPLSG
AITVIGHMDFEESRAHKIPVEAVDKGFPPLAGHCTLLVEVVDVNDNAPQLTIKTLSVPVK
EDAQLGTVIALISVIDLDADANGQVTCSLTPHVPFKLVSTYKNYYSLVLDRALDRESVSA
YELVVTARDGGSPSLWATARVSVEVADVNDNAPAFAQSEYTVFVKENNPPGCHIFTVSAR
DADAQENALVSYSLVERRLGERSLSSYVSVHAESGKVYALQPLDHEELELLQFQVSARDA
GVPPLGSNVTLQVFVLDENDNAPALLTPRMRGTDGAVSEMVLRSVGAGVVVGKVRAVDAD
SGYNAWLSYELQPETASASIPFRVGLYTGEISTTRALDETDAPRQRLLVLVKDHGEPALT
ATATVLVSLVESGQAPKSSSRASVGATGPEVTLVDVNVYLIIAICAVSSLLVLTLLLYTV
LRCSAMPTEGECAPGKPTLVCSSAVGSWSYSQQRRQRVCSGEGKQKTDLMAFSPGLSPCA
GSTERTGEPSASSDSTGKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWP
TVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIR
QEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PCDHA7

Symbol Nomenclature :

PCDHA7

SYNONYM :

CNR4; CNRN4; CNRS4; CRNR4; PCDH-ALPHA7

Designation :

KIAA0345-like 7|PCDH-alpha-7|ortholog to mouse CNR4|protocadherin alpha-7

Full Nomenclature :

protocadherin alpha 7

GENEID :

56141

HGNC :

8673

MIM :

606313

Vega :

OTTHUMG00000163371

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516383      ATGTTATACTCAAGTCGAGGAGATCCAGAGGGTCAGCCTCTACTGCTCTCGCTTCTGATC
NM_018910.2       ATGGTGTGCCCGAATGGATACGACCCAGGGGGCCGACATCTACTGCTGTTTATTATAATT
NM_031857.1       ATGTTATACTCAAGTCGAGGAGATCCAGAGGGTCAGCCTCTACTGCTCTCGCTTCTGATC
                  *** *.*.* *.*.* ** ..** ****.*** *..*.********* *  .**.*.** 

HsCD00516383      CTCGCAATGTGGGTGGTGGGGAGCGGCCAGCTCCACTACTCCGTCCCGGAGGAAGCCGAA
NM_018910.2       CTAGCAGCTTGGGAGGCAGGGAGAGGCCAGCTCCACTACTCGGTCCCCGAGGAGGCTAAA
NM_031857.1       CTCGCAATGTGGGTGGTGGGGAGCGGCCAGCTCCACTACTCCGTCCCGGAGGAAGCCGAA
                  **.***.  ****:** .*****.***************** ***** *****.** .**

HsCD00516383      CACGGCACCTTCGTGGGCCGCATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTG
NM_018910.2       CATGGCAACTTCGTGGGCCGCATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTG
NM_031857.1       CACGGCACCTTCGTGGGCCGCATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTG
                  ** ****.****************************************************

HsCD00516383      CCGCGCCTGTTCCAGTTGGATTCCAAAGGCCGCGGGGACCTTCTGGAGGTAAATCTGCAG
NM_018910.2       CCGCGCCTGTTCCGGGCGGTGTGCAAATTCCGTGGGGATCTTCTGGAGGTAAATCTGCAG
NM_031857.1       CCGCGCCTGTTCCAGTTGGATTCCAAAGGCCGCGGGGACCTTCTGGAGGTAAATCTGCAG
                  *************.*  **: * ****  *** ***** *********************

HsCD00516383      AATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGGAGCTGTGCGGGCGGAGCGCG
NM_018910.2       AATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGGAGCTGTGCGGGCGGAGCGCG
NM_031857.1       AATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGGAGCTGTGCGGGCGGAGCGCG
                  ************************************************************

HsCD00516383      GAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTTCCATGTGGAC
NM_018910.2       GAGTGCAGCATCCACCTGGAGGTGATCGTGGAAAGGCCGCTGCAGGTTTTCCATGTGGAC
NM_031857.1       GAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTTCCATGTGGAC
                  *****************************.**.***************************

HsCD00516383      GTGGAGGTGAAGGACATTAACGACAACCCTCCAGTGTTCCCAGCGACACAAAAGAATCTG
NM_018910.2       GTGGAGGTGAAGGACATTAACGACAACCCTCCGGTGTTCCCAGCGACACAAAGGAATCTG
NM_031857.1       GTGGAGGTGAAGGACATTAACGACAACCCTCCAGTGTTCCCAGCGACACAAAAGAATCTG
                  ********************************.*******************.*******

HsCD00516383      TTCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCGTCCGATGCA
NM_018910.2       TTCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCGTCCGATGCA
NM_031857.1       TTCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCGTCCGATGCA
                  ************************************************************

HsCD00516383      GATATCGGGGAGAACGCCCTGCTCACTTACAGACTGAGCCCCAATGAGTATTTCTTCCTG
NM_018910.2       GATATCGGGGAGAACGCCCTGCTCACTTACAGACTGAGCCCCAATGAGTATTTCTTCCTG
NM_031857.1       GATATCGGGGAGAACGCCCTGCTCACTTACAGACTGAGCCCCAATGAGTATTTCTTCCTG
                  ************************************************************

HsCD00516383      GACGTGCCAACCAGCAACCAGCAGGTAAAACCTCTTGGACTTGTATTACGGAAACTTTTA
NM_018910.2       GACGTGCCAACCAGCAACCAGCAGGTAAAACCTCTTGGACTTGTATTACGGAAACTTTTA
NM_031857.1       GACGTGCCAACCAGCAACCAGCAGGTAAAACCTCTTGGACTTGTATTACGGAAACTTTTA
                  ************************************************************

HsCD00516383      GACAGAGAAGAAACTCCGGAGCTTCATTTATTGCTCACGGCCACCGATGGAGGCAAACCC
NM_018910.2       GACAGAGAAGAAACTCCGGAGCTTCATTTATTGCTCACGGCCACCGATGGAGGCAAACCC
NM_031857.1       GACAGAGAAGAAACTCCGGAGCTTCATTTATTGCTCACGGCCACCGATGGAGGCAAACCC
                  ************************************************************

HsCD00516383      GAGCTGACTGGCACCGTTCAATTACTCATCACGGTACTGGACAACAATGACAATGCCCCA
NM_018910.2       GAGCTGACTGGCACCGTTCAATTACTCATCACGGTACTGGACAACAATGACAATGCCCCA
NM_031857.1       GAGCTGACTGGCACCGTTCAATTACTCATCACGGTACTGGACAACAATGACAATGCCCCA
                  ************************************************************

HsCD00516383      GTGTTCGACAGAACCCTGTATACGGTGAAATTACCAGAAAACGTTTCTATCGGAACGCTG
NM_018910.2       GTGTTCGACAGAACCCTGTATACGGTGAAATTACCAGAAAACGTTTCTATCGGAACGCTG
NM_031857.1       GTGTTCGACAGAACCCTGTATACGGTGAAATTACCAGAAAACGTTTCTATCGGAACGCTG
                  ************************************************************

HsCD00516383      GTGATTCACCCCAATGCCTCAGATTTAGACGAAGGCTTGAATGGGGATATTATTTACTCC
NM_018910.2       GTGATTCACCCCAATGCCTCAGATTTAGACGAAGGCTTGAATGGGGATATTATTTACTCC
NM_031857.1       GTGATTCACCCCAATGCCTCAGATTTAGACGAAGGCTTGAATGGGGATATTATTTACTCC
                  ************************************************************

HsCD00516383      TTCTCCAGTGATGTTTCTCCAGATATAAAATCCAAGTTCCACATGGACCCCTTAAGTGGG
NM_018910.2       TTCTCCAGTGATGTTTCTCCAGATATAAAATCCAAGTTCCACATGGACCCCTTAAGTGGG
NM_031857.1       TTCTCCAGTGATGTTTCTCCAGATATAAAATCCAAGTTCCACATGGACCCCTTAAGTGGG
                  ************************************************************

HsCD00516383      GCAATCACAGTGATAGGACATATGGATTTTGAAGAAAGTAGAGCACACAAGATCCCAGTC
NM_018910.2       GCAATCACAGTGATAGGACATATGGATTTTGAAGAAAGTAGAGCACACAAGATCCCAGTC
NM_031857.1       GCAATCACAGTGATAGGACATATGGATTTTGAAGAAAGTAGAGCACACAAGATCCCAGTC
                  ************************************************************

HsCD00516383      GAGGCTGTCGATAAAGGCTTCCCACCCCTGGCTGGTCATTGTACACTTCTTGTGGAAGTT
NM_018910.2       GAGGCTGTCGATAAAGGCTTCCCACCCCTGGCTGGTCATTGTACAGTTCTTGTGGAAGTT
NM_031857.1       GAGGCTGTCGATAAAGGCTTCCCACCCCTGGCTGGTCATTGTACACTTCTTGTGGAAGTT
                  ********************************************* **************

HsCD00516383      GTGGATGTAAATGACAATGCTCCACAGTTGACTATCAAAACGCTCTCGGTTCCTGTAAAA
NM_018910.2       GTGGATGTAAATGACAATGCTCCACAGTTGACTCTCACTTCCCTGTCTCTCCCTATTCCA
NM_031857.1       GTGGATGTAAATGACAATGCTCCACAGTTGACTATCAAAACGCTCTCGGTTCCTGTAAAA
                  *********************************.***.::* ** **  * ***.*:..*

HsCD00516383      GAGGACGCACAACTGGGGACAGTTATTGCCCTGATTAGTGTGATCGACCTAGACGCAGAT
NM_018910.2       GAGGACGCCCAACCAGGTACCGTCATCACATTGATTAGCGTGTTTGACCGAGATTTTGGA
NM_031857.1       GAGGACGCACAACTGGGGACAGTTATTGCCCTGATTAGTGTGATCGACCTAGACGCAGAT
                  ********.**** .** **.** ** .*. ******* ***:* **** ***   :*.:

HsCD00516383      GCCAACGGGCAGGTTACCTGCTCCCTGACGCCCCACGTCCCCTTCAAGCTGGTGTCCACC
NM_018910.2       GTCAACGGACAGGTTACCTGCTCCCTGACGCCCCGCGTTCCCTTCAAGTTGGTGTCCACC
NM_031857.1       GCCAACGGGCAGGTTACCTGCTCCCTGACGCCCCACGTCCCCTTCAAGCTGGTGTCCACC
                  * ******.*************************.*** ********* ***********

HsCD00516383      TACAAGAATTACTACTCGTTGGTGCTGGACAGAGCTCTGGACCGCGAGAGTGTGTCCGCC
NM_018910.2       TTCAAGAATTACTATTCATTGGTGCTGGACAGCGCTCTGGACCGCGAGAGTGTGTCCGCC
NM_031857.1       TACAAGAATTACTACTCGTTGGTGCTGGACAGAGCTCTGGACCGCGAGAGTGTGTCCGCC
                  *:************ **.**************.***************************

HsCD00516383      TACGAGCTGGTGGTTACCGCGCGGGACGGGGGCTCGCCTTCACTGTGGGCCACGGCCAGG
NM_018910.2       TATGAGCTGGTGGTTACCGCGCGGGACGGGGGCTCGCCTTCTCTGTGGGCCACTGCTAGC
NM_031857.1       TACGAGCTGGTGGTTACCGCGCGGGACGGGGGCTCGCCTTCACTGTGGGCCACGGCCAGG
                  ** **************************************:*********** ** ** 

HsCD00516383      GTGTCTGTGGAGGTGGCCGACGTGAACGACAACGCACCAGCGTTCGCGCAGTCCGAGTAC
NM_018910.2       GTGTCCGTGGAGGTGGCCGACGTGAACGACAACGCCCCGGCGTTCGCGCAGCCCGAGTAT
NM_031857.1       GTGTCTGTGGAGGTGGCCGACGTGAACGACAACGCACCAGCGTTCGCGCAGTCCGAGTAC
                  ***** *****************************.**.************ ******* 

HsCD00516383      ACGGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGG
NM_018910.2       ACGGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACTGTGTCGGCGGGG
NM_031857.1       ACGGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACGGTGTCTGCGCGG
                  *********************************************** ***** *** **

HsCD00516383      GACGCTGACGCGCAGGAGAACGCCCTGGTGTCCTACTCGCTGGTGGAGCGGCGGTTGGGC
NM_018910.2       GACGCGGACGCGCAGAAGAACGCGCTGGTGTCCTACTCGCTGGTGGAGCTGCGGGTGGGC
NM_031857.1       GACGCTGACGCGCAGGAGAACGCCCTGGTGTCCTACTCGCTGGTGGAGCGGCGGTTGGGC
                  ***** *********.******* ************************* **** *****

HsCD00516383      GAGCGCTCGCTGTCGAGCTACGTGTCAGTGCACGCGGAGAGCGGCAAGGTGTACGCGCTG
NM_018910.2       GAGCGCGCGCTGTCGAGCTACGTGTCAGTGCACGCGGAGAGCGGCAAGGTGTACGCGCTG
NM_031857.1       GAGCGCTCGCTGTCGAGCTACGTGTCAGTGCACGCGGAGAGCGGCAAGGTGTACGCGCTG
                  ****** *****************************************************

HsCD00516383      CAGCCGTTGGACCACGAGGAGCTGGAGCTGCTACAGTTCCAGGTGAGCGCGCGCGACGCG
NM_018910.2       CAGCCGTTGGACCACGAGGAGCTGGAGCTGTTGCAGTTCCAGGTGAGCGCGCGCGATGCG
NM_031857.1       CAGCCGTTGGACCACGAGGAGCTGGAGCTGCTACAGTTCCAGGTGAGCGCGCGCGACGCG
                  ****************************** *.*********************** ***

HsCD00516383      GGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
NM_018910.2       GGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
NM_031857.1       GGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
                  ************************************************************

HsCD00516383      AATGCGCCGGCGCTGCTGACACCTCGGATGAGGGGCACTGACGGCGCAGTGAGCGAGATG
NM_018910.2       AACGCGCCGGCACTGCTGGCGCCTCGGGTGGGTGGCACTGGTGGCGCAGTGAGAGAGCTT
NM_031857.1       AATGCGCCGGCGCTGCTGACACCTCGGATGAGGGGCACTGACGGCGCAGTGAGCGAGATG
                  ** ********.******.*.******.**.* *******. ***********.***.* 

HsCD00516383      GTGCTGCGGTCGGTGGGCGCCGGCGTAGTGGTGGGGAAGGTGCGCGCAGTGGACGCCGAC
NM_018910.2       GTGCCGCGGTCTGTGGGCGCGGGCCATGTGGTGGCGAAGGTACGTGCAGTTGACGCTGAC
NM_031857.1       GTGCTGCGGTCGGTGGGCGCCGGCGTAGTGGTGGGGAAGGTGCGCGCAGTGGACGCCGAC
                  **** ****** ******** *** ::******* ******.** ***** ***** ***

HsCD00516383      TCGGGCTACAACGCGTGGCTTTCATACGAGCTGCAGCCAGAAACGGCCAGCGCGAGCATC
NM_018910.2       TCAGGCTACAACGCGTGGCTTTCGTATGAGTTGCAACCGGTGGCGGCCGGTGCGAGCATC
NM_031857.1       TCGGGCTACAACGCGTGGCTTTCATACGAGCTGCAGCCAGAAACGGCCAGCGCGAGCATC
                  **.********************.** *** ****.**.*:..*****.* *********

HsCD00516383      CCGTTCCGCGTGGGGCTGTACACGGGCGAGATCAGCACAACGCGTGCCCTGGACGAAACG
NM_018910.2       CCGTTCCGCGTGGGGCTGTACACTGGTGAGATCAGCACGACACGAGCCCTAGATGAGACG
NM_031857.1       CCGTTCCGCGTGGGGCTGTACACGGGCGAGATCAGCACAACGCGTGCCCTGGACGAAACG
                  *********************** ** ***********.**.**:*****.** **.***

HsCD00516383      GACGCACCGCGCCAGCGCCTACTGGTGCTGGTGAAAGACCACGGGGAGCCAGCGCTGACG
NM_018910.2       GACGCACCGCGCCACCGCCTTCTGGTGCTTGTGAAGGACCACGGGGAGCCCTCGCTGACA
NM_031857.1       GACGCACCGCGCCAGCGCCTACTGGTGCTGGTGAAAGACCACGGGGAGCCAGCGCTGACG
                  ************** *****:******** *****.**************. *******.

HsCD00516383      GCCACGGCCACTGTGCTGGTGTCGCTGGTGGAGAGCGGCCAGGCGCCAAAGTCATCGTCG
NM_018910.2       GCCACAGCCACCGTGCTGGTGTCGCTGGTGGAAAGCGGCCAGGCACCAAAGGCGTCGTCG
NM_031857.1       GCCACGGCCACTGTGCTGGTGTCGCTGGTGGAGAGCGGCCAGGCGCCAAAGTCATCGTCG
                  *****.***** ********************.***********.****** *.******

HsCD00516383      CGGGCGTCAGTGGGTGCCACGGGCCCCGAGGTGACGCTGGTGGATGTCAACGTGTACCTG
NM_018910.2       CGGGCATCGTTGGGCATTGCAGGCCCAGAGACCGAGCTGGTGGATGTCAACGTGTACCTG
NM_031857.1       CGGGCGTCAGTGGGTGCCACGGGCCCCGAGGTGACGCTGGTGGATGTCAACGTGTACCTG
                  *****.**. **** .  .*.*****.***.  ..*************************

HsCD00516383      ATCATCGCCATCTGCGCGGTGTCTAGCCTGTTGGTTCTCACGCTGCTGCTGTACACTGTG
NM_018910.2       ATCATCGCCATCTGCGCGGTGTCCAGTCTGTTGGTGCTTACCCTGCTGCTGTACACGGCG
NM_031857.1       ATCATCGCCATCTGCGCGGTGTCTAGCCTGTTGGTTCTCACGCTGCTGCTGTACACTGTG
                  *********************** ** ******** ** ** ************** * *

HsCD00516383      CTGCGGTGCTCGGCGATGCCCACCGAGGGCGAGTGCGCGCCTGGCAAGCCGACGCTGGTG
NM_018910.2       TTGCGGTGCTCAGCGCCGTCCTCTGAGGGCGCATGTAGTTTGGTAAAGCCCACTCTGGTG
NM_031857.1       CTGCGGTGCTCGGCGATGCCCACCGAGGGCGAGTGCGCGCCTGGCAAGCCGACGCTGGTG
                   **********.***. * **:* *******..** .     * .***** ** ******

HsCD00516383      TGTTCTAGCGCGGTGGGGAGTTGGTCGTACTCGCAGCAGAGGAGGCAGAGGGTGTGCTCT
NM_018910.2       TGCTCCAGCGCGGTGGGGAGCTGGTCATTCTCCCAGCAGAGGCGGCAGAGGGTGTGCTCT
NM_031857.1       TGTTCTAGCGCGGTGGGGAGTTGGTCGTACTCGCAGCAGAGGAGGCAGAGGGTGTGCTCT
                  ** ** ************** *****.*:*** *********.*****************

HsCD00516383      GGCGAGGGTAAGCAGAAGACCGACCTCATGGCCTTCAGCCCGGGCCTTTCTCCTTGTGCT
NM_018910.2       GGGGAGGGCCCACCCAAGACAGACCTCATGGCCTTCAGTCCCAGCCTTCCTCAGG-----
NM_031857.1       GGCGAGGGTAAGCAGAAGACCGACCTCATGGCCTTCAGCCCGGGCCTTTCTCCTTGTGCT
                  ** ***** ...*. *****.***************** ** .***** ***.       

HsCD00516383      GGATCTACAGAGCGAACGGGAGAACCCTCTGCTTCCTCAGATTCAACTGGGAAGCCACGA
NM_018910.2       ----------------------------------GTCCATCCTCTACAGACAATCCACGA
NM_031857.1       GGATCTACAGAGCGAACGGGAGAACCCTCTGCTTCCTCAGATTCAACTGGGAAGCCACGA
                                                       ** . **:**:*. ** ******

HsCD00516383      CAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTG
NM_018910.2       CAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTG
NM_031857.1       CAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTG
                  ************************************************************

HsCD00516383      CACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCA
NM_018910.2       CACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCA
NM_031857.1       CACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCA
                  ************************************************************

HsCD00516383      ACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCG
NM_018910.2       ACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCG
NM_031857.1       ACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCG
                  ************************************************************

HsCD00516383      GGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGT
NM_018910.2       GGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGT
NM_031857.1       GGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGT
                  ************************************************************

HsCD00516383      CCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGG
NM_018910.2       CCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGG
NM_031857.1       CCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGG
                  ************************************************************

HsCD00516383      CAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAG
NM_018910.2       CAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAG
NM_031857.1       CAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAG
                  ************************************************************

HsCD00516383      GAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAA
NM_018910.2       GAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAA
NM_031857.1       GAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAA
                  ************************************************************

HsCD00516383      GGGAACAGCACGACTGACAACAGTGACCAG---
NM_018910.2       GGGAACAGCACGACTGACAACAGTGACCAGTGA
NM_031857.1       GGGAACAGCACGACTGACAACAGTGACCAGTGA
                  ******************************