SART3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SART3
Gene Name:  squamous cell carcinoma antigen recognized by T cells 3
Original Clone ID:
ccsbBroadEn_07477
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC111983.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516388
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2889nts
Open reading frame: 1 to 2889

ATGGCGACTGCGGCCGAAACCTCGGCTTCAGAACCCGAGGCTGAGTCCAAGGCTGGGCCC
AAGGCTGACGGAGAGGAGGATGAGGTTAAGGCGGCTAGGACAAGGAGAAAGGTGTTATCG
CGGGCTGTGGCCGCTGCGACATACAAGACCATGGGGCCAGCGTGGGATCAGCAGGAGGAA
GGCGTGAGCGAGAGCGATGGGGATGAGTACGCCATGGCTTCCTCCGCGGAGAGCTCCCCC
GGGGAGTACGAGTGGGAATATGACGAAGAGGAGGAGAAAAACCAGCTGGAGATTGAGAGA
CTGGAGGAGCAGTTGTCTATCAACGTCTATGACTACAACTGCCATGTGGACTTGATCAGA
CTGCTCAGGCTGGAAGGGGAGCTTACCAAGGTGAGGATGGCCCGCCAGAAGATGAGTGAA
ATCTTTCCCTTGACTGAAGAGCTCTGGCTGGAGTGGCTGCATGACGAGATCAGCATGGCC
CAGGATGGCCTGGACAGAGAGCACGTGTATGACCTCTTTGAGAAAGCCGTGAAGGATTAC
ATTTGTCCTAACATTTGGCTAGAGTATGGCCAGTACTCAGTTGGTGGGATTGGTCAGAAA
GGTGGCCTTGAGAAAGTTCGCTCCGTGTTTGAAAGGGCTCTCTCGTCTGTTGGTTTACAT
ATGACCAAAGGACTCGCCCTCTGGGAGGCTTACCGAGAGTTTGAAAGTGCGATTGTGGAA
GCTGCTCGGCTTGAGAAAGTCCACAGTCTTTTCCGGCGACAGTTGGCGATCCCACTCTAT
GATATGGAGGCCACATTTGCAGAGTATGAAGAATGGTCAGAAGACCCAATACCAGAGTCA
GTAATTCAGAACTATAACAAAGCACTACAGCAGCTGGAGAAATATAAACCCTATGAAGAA
GCACTGTTGCAGGCAGAGGCACCAAGGCTGGCAGAATATCAAGCATATATCGATTTTGAG
ATGAAAATTGGCGATCCTGCTCGCATTCAGTTGATCTTTGAGCGCGCCCTGGTCGAGAAC
TGCCTTGTCCCAGACTTATGGATCCGTTACAGTCAGTACCTAGATCGACAACTGAAAGTA
AAGGATTTGGTTTTATCTGTACATAACCGCGCTATTAGAAACTGCCCCTGGACAGTTGCC
TTATGGAGTCGGTACCTCTTGGCCATGGAGAGACATGGAGTTGATCATCAAGTAATTTCT
GTAACCTTCGAGAAAGCTTTGAATGCCGGCTTCATCCAGGCCACTGATTATGTGGAGATT
TGGCAGGCATACCTTGATTACCTGAGGAGAAGGGTTGATTTCAAACAAGACTCCAGTAAA
GAGCTGGAGGAGTTGAGGGCCGCCTTTACTCGTGCCTTGGAGTATCTGAAGCAGGAGGTG
GAAGAGCGTTTCAATGAGAGTGGTGATCCAAGCTGCGTGATTATGCAGAACTGGGCTAGG
ATTGAGGCTCGACTGTGCAATAACATGCAGAAAGCTCGGGAACTCTGGGATAGCATCATG
ACCAGAGGAAATGCCAAGTACGCCAACATGTGGCTAGAGTATTACAACCTGGAAAGAGCT
CATGGTGACACCCAGCACTGCCGGAAGGCTCTGCACCGGGCCGTCCAGTGCACCAGTGAC
TACCCAGAGCACGTCTGCGAAGTGTTACTCACCATGGAGAGGACAGAAGGTTCTTTAGAA
GATTGGGATATAGCTGTTCAGAAAACTGAAACCCGATTAGCTCGTGTCAATGAGCAGAGA
ATGAAGGCTGCAGAGAAGGAAGCAGCCCTTGTGCAGCAAGAAGAAGAAAAGGCTGAACAA
CGGAAAAGAGCTCGGGCTGAGAAGAAAGCGTTAAAAAAGAAGAAAAAGATCAGAGGCCCA
GAGAAGCGCGGAGCAGATGAGGACGATGAGAAAGAGTGGGGCGATGATGAAGAAGAGCAG
CCTTCCAAACGCAGAAGGGTCGAGAACAGCATCCCTGCAGCTGGAGAAACACAAAATGTA
GAAGTAGCAGCAGGGCCCGCTGGGAAATGTGCTGCCGTAGATGTGGAGCCCCCTTCGAAG
CAGAAGGAGAAGGCAGCCTCCCTGAAGAGGGACATGCCCAAGGTGCTGCACGACAGCAGC
AAGGACAGCATCACCGTCTTTGTCAGCAACCTGCCCTACAGCATGCAGGAGCCGGACACG
AAGCTCAGGCCACTCTTCGAGGCCTGTGGGGAGGTGGTCCAGATCCGACCCATCTTCAGC
AACCGTGGGGATTTCCGAGGTTACTGCTACGTGGAGTTTAAAGAAGAGAAATCAGCCCTT
CAGGCACTGGAGATGGACCGGAAAAGTGTAGAAGGGAGGCCAATGTTTGTTTCCCCCTGT
GTGGATAAGAGCAAAAACCCCGATTTTAAGGTGTTCAGGTACAGCACTTCCCTAGAGAAA
CACAAGCTGTTCATCTCAGGCCTGCCTTTCTCCTGTACTAAAGAGGAACTAGAAGAAATC
TGTAAGGCTCATGGCACCGTGAAGGACCTCAGGCTGGTCACCAACCGGGCTGGCAAACCA
AAGGGCCTGGCCTACGTGGAGTATGAAAATGAATCCCAGGCGTCGCAGGCTGTGATGAAG
ATGGACGGCATGACTATCAAAGAGAACATCATCAAAGTGGCAATCAGCAACCCTCCTCAG
AGGAAAGTTCCAGAGAAGCCAGAGACCAGGAAGGCACCAGGTGGCCCCATGCTTTTGCCG
CAGACATACGGAGCGAGGGGGAAGGGAAGGACGCAGCTGTCTCTACTGCCTCGTGCCCTG
CAGCGCCCAAGTGCTGCAGCTCCTCAGGCTGAGAACGGCCCTGCCGCGGCTCCTGCAGTT
GCCGCCCCAGCAGCCACCGAGGCACCCAAGATGTCCAATGCCGATTTTGCCAAGCTGTTT
CTGAGAAAG

Protein sequence predicted based on nucleotide sequence

MATAAETSASEPEAESKAGPKADGEEDEVKAARTRRKVLSRAVAAATYKTMGPAWDQQEE
GVSESDGDEYAMASSAESSPGEYEWEYDEEEEKNQLEIERLEEQLSINVYDYNCHVDLIR
LLRLEGELTKVRMARQKMSEIFPLTEELWLEWLHDEISMAQDGLDREHVYDLFEKAVKDY
ICPNIWLEYGQYSVGGIGQKGGLEKVRSVFERALSSVGLHMTKGLALWEAYREFESAIVE
AARLEKVHSLFRRQLAIPLYDMEATFAEYEEWSEDPIPESVIQNYNKALQQLEKYKPYEE
ALLQAEAPRLAEYQAYIDFEMKIGDPARIQLIFERALVENCLVPDLWIRYSQYLDRQLKV
KDLVLSVHNRAIRNCPWTVALWSRYLLAMERHGVDHQVISVTFEKALNAGFIQATDYVEI
WQAYLDYLRRRVDFKQDSSKELEELRAAFTRALEYLKQEVEERFNESGDPSCVIMQNWAR
IEARLCNNMQKARELWDSIMTRGNAKYANMWLEYYNLERAHGDTQHCRKALHRAVQCTSD
YPEHVCEVLLTMERTEGSLEDWDIAVQKTETRLARVNEQRMKAAEKEAALVQQEEEKAEQ
RKRARAEKKALKKKKKIRGPEKRGADEDDEKEWGDDEEEQPSKRRRVENSIPAAGETQNV
EVAAGPAGKCAAVDVEPPSKQKEKAASLKRDMPKVLHDSSKDSITVFVSNLPYSMQEPDT
KLRPLFEACGEVVQIRPIFSNRGDFRGYCYVEFKEEKSALQALEMDRKSVEGRPMFVSPC
VDKSKNPDFKVFRYSTSLEKHKLFISGLPFSCTKEELEEICKAHGTVKDLRLVTNRAGKP
KGLAYVEYENESQASQAVMKMDGMTIKENIIKVAISNPPQRKVPEKPETRKAPGGPMLLP
QTYGARGKGRTQLSLLPRALQRPSAAAPQAENGPAAAPAVAAPAATEAPKMSNADFAKLF
LRK

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable