Gene:
Gene Symbol:
SUGP2
Gene Name:
SURP and G patch domain containing 2
Original Clone ID:
ccsbBroadEn_11459
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC020586.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516392
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTATGATGACGTCCACAGCGATGGCAGATACTCCCTCAGTGGATCTGTAGCTCACTCT
AGAGATGCCGGAAGAGAAGGCCTGAGAAGTGACGTATTTCCAGGGCCTTCCTTCAGATCA
AGCAACCCTTCCATCAGTGATGACAGCTACTTTCGCAAAGAATGTGGCCGGGATCTGGAA
TTTTCTCACTCTGATTCTCGGGACCAGGTCATTGGCCACCGGAAATTGGGGCATTTCCGT
TCTCAGGACTGGAAATTTGCGCTCCGTGGTTCTTGGGAACAAGACTTTGGCCATCCAGTT
TCTCAAGAGTCCTCTTGGTCACAGGAGTATAGTTTTGGTCCCTCTGCAGTTTTGGGGGAC
TTTGGATCTTCCAGGCTGATTGAGAAAGAGTGTTTGGAGAAGGAGAGTCGGGATTATGAC
GTGGACCATCCTGGGGAGGCTGACTCTGTGCTTAGGGGCAGCAGTCAAGTCCAGGCCAGA
GGTCGAGCTCTAAACATCGTTGACCAGGAAGGTTCCCTCCTAGGAAAGGGGGAGACTCAG
GGCCTGCTCACAGCTAAGGGGGGTGTTGGGAAACTTGTCACATTGAGAAATGTGAGCACA
AAAAAAATACCCACCGTGAATCGTATTACTCCCAAAACTCAGGGCACTAACCAAATCCAG
AAAAACACTCCAAGTCCTGATGTGACCCTGGGGACAAACCCAGGGACAGAAGATATCCAG
TTCCCCATTCAGAAGATCCCTCTGGGGCTGGATCTGAAGAATCTTCGGCTCCCCAGAAGA
AAGATGAGCTTTGACATCATAGATAAGTCTGATGTTTTTTCAAGATTTGGGATAGAAATA
ATCAAATGGGCAGGATTCCACACCATAAAAGATGATATTAAATTTTCCCAACTTTTCCAG
ACTCTCTTTGAACTTGAAACAGAAACCTGTGCTAAAATGCTTGCCTCATTCAAATGTTCC
TTAAAACCAGAGCACAGAGATTTTTGCTTTTTTACTATCAAATTTTTAAAGCACTCTGCT
TTGAAAACACCCAGAGTTGATAATGAGTTTTTAAACATGCTTTTAGACAAAGGTGCTGTG
AAGACCAAAAATTGCTTTTTTGAAATCATAAAGCCTTTTGACAAGTACATAATGAGACTT
CAAGACCGGCTTCTGAAGAGTGTCACACCTTTGCTTATGGCCTGCAATGCCTACGAGCTA
AGTGTCAAGATGAAGACCCTCAGTAACCCCCTGGACTTGGCTCTTGCCCTAGAAACCACC
AACTCTCTCTGCCGGAAGTCTTTGGCCCTTTTGGGACAGACATTTTCCTTGGCCTCTTCT
TTCCGGCAGGAGAAAATCTTAGAAGCTGTCGGCCTGCAAGATATAGCTCCCTCACCTGCT
GCGTTTCCAAACTTCGAAGACTCCACTTTGTTTGGGCGAGAGTACATAGACCACCTGAAG
GCCTGGCTAGTCAGCAGCGGATGTCCCCTCCAGGTTAAGAAAGCCGAACCAGAGCCGATG
CGAGAGGAGGAGAAAATGATTCCTCCTACGAAACCTGAAATTCAGGCCAAGGCTCCAAGT
AGTCTGAGTGATGCTGTCCCCCAGCGAGCAGATCACAGGGTAGTGGGCACCATCGACCAG
CTTGTGAAACGTGTCATCGAAGGCAGCCTGTCTCCCAAAGAGAGAACTCTTCTCAAAGAG
GACCCTGCTTACTGGTTTTTGTCTGATGAAAATAGTCTGGAGTATAAATATTACAAGCTG
AAGTTGGCAGAAATGCAGCGGATGAGCGAGAACTTGCGAGGAGCCGACCAGAAGCCGACC
TCAGCAGACTGTGCAGTGAGGGCCATGCTGTACTCCCGGGCTGTCCGCAACCTCAAGAAG
AAACTCCTTCCGTGGCAGCGGCGGGGGCTCCTCCGTGCTCAAGGGCTCCGGGGCTGGAAG
GCGAGGAGAGCGACCACCGGGACCCAGACCCTCCTATCCTCAGGCACCAGGCTGAAACAC
CACGGCCGGCAGGCTCCAGGCCTCTCACAGGCAAAACCATCCCTGCCAGACAGAAATGAT
GCTGCCAAGGACTGCCCGCCAGACCCAGTTGGACCTTCTCCTCAGGACCCCAGCTTAGAA
GCCTCAGGCCCATCCCCCAAGCCAGCAGGAGTGGACATCTCTGAAGCACCTCAGACCTCT
TCTCCCTGCCCATCTGCTGACATTGACATGAAGACAATGGAGACTGCAGAGAAACTGGCT
AGATTTGTTGCTCAGGTGGGACCAGAGATCGAACAATTCAGCATAGAAAACAGCACCGAT
AACCCTGACCTGTGGTTTCTACATGACCAAAATAGTTCTGCTTTCAAATTCTATCGAAAG
AAAGTGTTTGAACTATGTCCATCAATTTGTTTCACGTCATCTCCGCACAACCTTCACACT
GGTGGTGGTGACACCACGGGTTCTCAGGAGAGCCCCGTGGACCTCATGGAAGGGGAAGCA
GAGTTTGAAGACGAGCCCCCTCCGCGGGAGGCTGAGCTGGAGAGCCCAGAGGCCTCCTCA
GGTACCTGCTTCCCTCGGAAGAGGATCAGCAGCAAGTCATTGAAGGTTGGCATGATTCCA
GCTCCCAAGAGAGTGTGTCTCATCCAGGAGCCAAAAGTCCATGAACCAGTTCGAATTGCC
TATGACAGGCCTCGGGGTCGTCCCATGTCCAAAAAGAAGAAACCCAAGGACTTGGACTTC
GCCCAGCAGAAGCTGACCGATAAGAACCTGGGCTTCCAGATGCTGCAGAAGATGGGCTGG
AAGGAGGGCCATGGCCTGGGCTCCCTCGGAAAGGGCATCAGGGAGCCGGTCAGCGTGTAC
GCAGCAGGCAGCCTGGGGTGGGAGTGGGTGGGGCCTCAGTCCTTCCACCTGCAGCCTGCC
GCTTGGCTCCTTCACAGCCAAGATGGCTTACAGCTGGCAGTTGATTTTTGTTTTTTAAAC
AGAAGGCATCTTCAGATGAGAAGC
Protein sequence predicted based on nucleotide sequence
MYDDVHSDGRYSLSGSVAHSRDAGREGLRSDVFPGPSFRSSNPSISDDSYFRKECGRDLE FSHSDSRDQVIGHRKLGHFRSQDWKFALRGSWEQDFGHPVSQESSWSQEYSFGPSAVLGD FGSSRLIEKECLEKESRDYDVDHPGEADSVLRGSSQVQARGRALNIVDQEGSLLGKGETQ GLLTAKGGVGKLVTLRNVSTKKIPTVNRITPKTQGTNQIQKNTPSPDVTLGTNPGTEDIQ FPIQKIPLGLDLKNLRLPRRKMSFDIIDKSDVFSRFGIEIIKWAGFHTIKDDIKFSQLFQ TLFELETETCAKMLASFKCSLKPEHRDFCFFTIKFLKHSALKTPRVDNEFLNMLLDKGAV KTKNCFFEIIKPFDKYIMRLQDRLLKSVTPLLMACNAYELSVKMKTLSNPLDLALALETT NSLCRKSLALLGQTFSLASSFRQEKILEAVGLQDIAPSPAAFPNFEDSTLFGREYIDHLK AWLVSSGCPLQVKKAEPEPMREEEKMIPPTKPEIQAKAPSSLSDAVPQRADHRVVGTIDQ LVKRVIEGSLSPKERTLLKEDPAYWFLSDENSLEYKYYKLKLAEMQRMSENLRGADQKPT SADCAVRAMLYSRAVRNLKKKLLPWQRRGLLRAQGLRGWKARRATTGTQTLLSSGTRLKH HGRQAPGLSQAKPSLPDRNDAAKDCPPDPVGPSPQDPSLEASGPSPKPAGVDISEAPQTS SPCPSADIDMKTMETAEKLARFVAQVGPEIEQFSIENSTDNPDLWFLHDQNSSAFKFYRK KVFELCPSICFTSSPHNLHTGGGDTTGSQESPVDLMEGEAEFEDEPPPREAELESPEASS GTCFPRKRISSKSLKVGMIPAPKRVCLIQEPKVHEPVRIAYDRPRGRPMSKKKKPKDLDF AQQKLTDKNLGFQMLQKMGWKEGHGLGSLGKGIREPVSVYAAGSLGWEWVGPQSFHLQPA AWLLHSQDGLQLAVDFCFLNRRHLQMRS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
