Gene:
Gene Symbol:
PHF20
Gene Name:
PHD finger protein 20
Original Clone ID:
ccsbBroadEn_08258
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC150178.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516399
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACAAAGCATCCACCTAACAGACGAGGAATCAGCTTTGAAGTGGGAGCCCAGTTGGAA
GCCCGGGACCGTTTAAAAAACTGGTATCCAGCTCACATAGAAGACATTGACTACGAGGAA
GGAAAAGTACTCATCCATTTCAAGCGTTGGAACCATCGTTATGATGAGTGGTTCTGCTGG
GACAGTCCTTATTTACGCCCTTTAGAGAAAATACAGCTGAGGAAAGAGGGCTTGCATGAA
GAGGATGGATCTTCTGAATTTCAAATAAATGAGCAGGTCCTTGCTTGCTGGTCTGATTGT
CGTTTTTACCCGGCCAAAGTCACTGCTGTTAACAAGGATGGTACTTACACTGTGAAATTT
TATGATGGAGTAGTTCAGACTGTCAAACATATTCATGTCAAAGCTTTTTCCAAAGATCAG
AATATTGTGGGTAATGCTAGGCCTAAAGAAACAGATCACAAAAGTCTTTCATCATCTCCT
GATAAACGAGAGAAGTTTAAAGAACAGAGAAAAGCAACAGTGAATGTGAAGAAAGACAAA
GAAGATAAACCCTTAAAGACAGAAAAGCGACCCAAGCAGCCTGATAAAGAAGGAAAGTTA
ATCTGTTCTGAAAAGGGGAAAGTGTCAGAGAAAAGTCTTCCCAAGAACGAGAAGGAAGAC
AAGGAAAACATTTCCGAAAATGACAGAGAGTATTCTGGAGATGCCCAAGTGGATAAGAAA
CCTGAAAATGACATTGTGAAGAGTCCACAAGAAAACTTGAGGGAACCCAAAAGAAAACGA
GGCAGACCCCCTTCCATAGCTCCTACTGCTGTGGATTCAAACTCTCAAACTTTGCAACCA
ATAACATTGGAACTGAGAAGAAGGAAAATATCAAAAGGATGTGAAGTCCCATTAAAACGT
CCTCGGCTTGACAAAAATTCATCCCAGGAAAAGTCAAAAAACTACTCGGAAAACACTGAC
AAAGACTTATCGAGGAGACGTTCCTCCAGGCTGTCCACTAATGGGACCCATGAGATCCTA
GATCCTGACTTGGTTGTATCAGATTTGGTTGATACGGATCCTTTGCAAGACACGTTGTCT
AGTACCAAGGAATCTGAAGAAGGTCAGTTGAAGTCTGCTTTGGAAGCTGGCCAGGTCTCA
TCTGCACTGACTTGCCACTCCTTTGGGGATGGATCCGGGGCTGCAGGCTTGGAGTTGAAC
TGCCCATCAATGGGAGAAAACACGATGAAAACAGAACCGACTTCTCCCCTTGTGGAATTA
CAAGAGATTTCGACTGTGGAAGTAACAAATACTTTTAAGAAAACAGATGATTTTGGGTCA
TCTAATGCACCAGCTGTCGACCTAGACCATAAGTTTAGATGCAAAGTTGTGGACTGTTTA
AAATTTTTCCGCAAAGCCAAACTGTTGCACTATCACATGAAGTATTTCCATGGAATGGAG
AAGTCACTGGAGCCAGAAGAGAGCCCGGGAAAGAGGCATGTCCAAACCAGGGGCCCTTCA
GCTTCAGACAAGCCCAGCCAGGAGACCCTGACCAGGAAGCGGGTCTCTGCCAGTTCCCCA
ACTACAAAAGACAAGGAAAAGAATAAAGAGAAGAAATTCAAGGAGTTTGTGAGAGTGAAG
CCAAAGAAGAAAAAGAAAAAGAAAAAGAAAACCAAACCTGAATGCCCCTGCAGTGAGGAG
ATCAGTGACACCTCCCAGGAACCTTCTCCACCCAAGGCATTTGCTGTTACCAGGTGTGGG
TCCTCACACAAGCCAGGGGTCCATATGAGCCCGCAGCTTCATGGCCCAGAATCTGGACAC
CACAAAGGGAAAATGAAAGCATTGGAGGAGGATAATTTGAGTGAGTCCTCTTCTGAGAGC
TTTCTCTGGAGTGATGATGAGTATGGCCAAGATGTGGATGTGACCACCAACCCAGATGAG
GAACTTGATGGGGATGACCGCTATGACTTCGAGGTGGTCCGCTGCATCTGTGAGGTCCAG
GAGGAAAATGACTTCATGATTCAGTGTGAAGAGTGCCAGTGCTGGCAGCATGGGGTCTGC
ATGGGATTACTGGAAGAAAATGTGCCCGAGAAATACACCTGTTATGTTTGCCAAGACCCT
CCAGGTCAGAGGCCTGGCTTCAAGTACTGGTATGACAAGGAGTGGCTGAGCAGGGGACAT
ATGCATGGCCTGGCATTTCTAGAAGAGAACTACTCCCATCAGAATGCCAAGAAGATCGTG
GCCACCCACCAGCTTCTTGGTGATGTGCAGAGAGTGATTGAGGTTCTGCATGGCCTGCAG
CTCAAGATGAGCATCTTGCAAAGCCGGGAGCATCCTGATCTGCCGCTGTGGTGCCAGCCT
TGGAAACAGCACTCAGGGGAGGGGAGATCTCATTTCAGAAACATCCCTGTCACTGACACC
AGGAGCAAGGAGGAAGCTCCAAGCTATAGAACTTTGAACGGGGCAGTGGAGAAGCCCAGG
CCCCTGGCCCTGCCCCTGCCGCGTTCTGTGGAGGAATCCTATATCACCAGTGAGCATTGC
TACCAGAAGCCCCGCGCCTATTACCCTGCCGTGGAGCAGAAGCTGGTGGTGGAGACGAGG
GGCTCTGCCCTCGACGATGCGGTCAACCCCCTCCATGAGAACGGCGATGATTCCCTTTCC
CCGCGCCTGGGCTGGCCTCTAGACCAAGACAGGAGCAAGGGGGACAGTGACCCCAAACCC
GGCTCCCCAAAGGTGAAGGAATATGTCTCCAAAAAGGCCCTACCAGAAGAAGCCCCTGCT
CGGAAGCTGCTGGACAGAGGTGGAGAGGGGCTGCTGAGCTCCCAGCACCAGTGGCAGTTT
AACCTGCTGACCCATGTGGAATCTCTTCAGGATGAAGTTACGCACAGGATGGACTCCATT
GAGAAGGAGTTGGATGTGTTGGAGAGCTGGCTGGACTACACTGGGGAACTGGAGCCCCCT
GAGCCGCTGGCCAGGCTTCCGCAGCTCAAGCATTGTATCAAGCAGCTGCTGATGGACCTG
GGCAAGGTGCAGCAGATCGCCCTCTGCTGCTCAACA
Protein sequence predicted based on nucleotide sequence
MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGKVLIHFKRWNHRYDEWFCW DSPYLRPLEKIQLRKEGLHEEDGSSEFQINEQVLACWSDCRFYPAKVTAVNKDGTYTVKF YDGVVQTVKHIHVKAFSKDQNIVGNARPKETDHKSLSSSPDKREKFKEQRKATVNVKKDK EDKPLKTEKRPKQPDKEGKLICSEKGKVSEKSLPKNEKEDKENISENDREYSGDAQVDKK PENDIVKSPQENLREPKRKRGRPPSIAPTAVDSNSQTLQPITLELRRRKISKGCEVPLKR PRLDKNSSQEKSKNYSENTDKDLSRRRSSRLSTNGTHEILDPDLVVSDLVDTDPLQDTLS STKESEEGQLKSALEAGQVSSALTCHSFGDGSGAAGLELNCPSMGENTMKTEPTSPLVEL QEISTVEVTNTFKKTDDFGSSNAPAVDLDHKFRCKVVDCLKFFRKAKLLHYHMKYFHGME KSLEPEESPGKRHVQTRGPSASDKPSQETLTRKRVSASSPTTKDKEKNKEKKFKEFVRVK PKKKKKKKKKTKPECPCSEEISDTSQEPSPPKAFAVTRCGSSHKPGVHMSPQLHGPESGH HKGKMKALEEDNLSESSSESFLWSDDEYGQDVDVTTNPDEELDGDDRYDFEVVRCICEVQ EENDFMIQCEECQCWQHGVCMGLLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGH MHGLAFLEENYSHQNAKKIVATHQLLGDVQRVIEVLHGLQLKMSILQSREHPDLPLWCQP WKQHSGEGRSHFRNIPVTDTRSKEEAPSYRTLNGAVEKPRPLALPLPRSVEESYITSEHC YQKPRAYYPAVEQKLVVETRGSALDDAVNPLHENGDDSLSPRLGWPLDQDRSKGDSDPKP GSPKVKEYVSKKALPEEAPARKLLDRGGEGLLSSQHQWQFNLLTHVESLQDEVTHRMDSI EKELDVLESWLDYTGELEPPEPLARLPQLKHCIKQLLMDLGKVQQIALCCST
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
