Gene:
Gene Symbol:
DAB2IP
Gene Name:
DAB2 interacting protein
Original Clone ID:
ccsbBroadEn_13291
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC146762.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516406
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCGAGGCTGAAGGAGTCTCGCTCCCACGAGTCCCTGCTCAGCCCCAGCAGTGCGGTG
GAGGCGCTGGACCTCAGCATGGAGGAAGAGGTGGTCATCAAGCCCGTGCACAGCAGCATC
CTTGGCCAGGACTACTGCTTCGAGGTGACGACGTCATCAGGAAGCAAGTGCTTTTCCTGC
CGGTCTGCAGCTGAGCGGGATAAGTGGATGGAGAACCTCCGGCGAGCGGTGCATCCCAAC
AAGGACAACAGCCGGCGTGTGGAGCACATCCTGAAGCTGTGGGTGATCGAGGCCAAGGAC
CTGCCAGCCAAGAAGAAGTACCTGTGCGAGCTGTGCCTGGACGATGTGCTCTATGCCCGC
ACCACGGGCAAGCTCAAGACGGACAATGTTTTCTGGGGCGAGCACTTCGAGTTCCACAAC
TTGCCGCCTCTGCGCACGGTCACTGTCCACCTGTACCGGGAGACCGACAAGAAGAAGAAG
AAGGAGCGCAACAGTTACCTGGGCCTGGTGAGCCTACCTGCTGCCTCGGTGGCCGGGCGG
CAGTTCGTGGAGAAGTGGTACCCGGTGGTGACGCCCAACCCCAAGGGCGGCAAGGGCCCT
GGACCCATGATCCGCATCAAGGCGCGCTACCAAACCATCACCATCCTGCCCATGGAGATG
TACAAAGAGTTCGCTGAGCACATCACCAACCACTACCTGGGGCTGTGTGCAGCCCTCGAG
CCCATCCTCAGTGCCAAGACCAAGGAGGAGATGGCATCTGCCCTGGTGCACATCCTGCAG
AGCACGGGCAAGGTGAAGGACTTCCTGACAGACCTGATGATGTCAGAGGTGGACCGCTGC
GGGGACAACGAGCACCTCATCTTCCGGGAGAACACACTGGCCACCAAGGCCATTGAGGAG
TACCTCAAGCTAGTGGGCCAGAAGTACCTGCAGGACGCCCTAGGTGAGTTCATCAAAGCG
CTGTATGAGTCAGATGAGAACTGCGAAGTGGATCCCAGCAAGTGCTCGGCCGCTGACCTC
CCAGAGCACCAGGGCAACCTCAAGATGTGCTGCGAGCTGGCCTTCTGCAAGATCATCAAC
TCCTACTGTGTCTTCCCACGGGAGTTGAAAGAGGTGTTTGCCTCGTGGAGGCAGGAGTGC
AGCAGTCGCGGCCGCCCGGACATCAGTGAGCGGCTCATCAGCGCCTCCCTCTTCCTGCGC
TTCCTCTGCCCAGCCATCATGTCGCCCTCACTCTTCAACCTGCTGCAGGAGTACCCTGAT
GACCGCACTGCCCGCACCCTCACCCTCATCGCCAAGGTCACCCAGAACCTGGCCAACTTT
GCCAAATTTGGCAGCAAGGAGGAATACATGTCCTTCATGAACCAGTTCCTAGAGCATGAG
TGGACCAACATGCAGCGCTTCCTGCTGGAGATCTCCAACCCCGAGACCCTCTCCAATACA
GCCGGCTTCGAGGGCTACATCGACCTGGGCCGCGAGCTCTCCAGCCTGCACTCACTGCTC
TGGGAGGCCGTCAGCCAGCTGGAGCAGAGCATAGTATCCAAACTGGGACCCCTGCCTCGG
ATCCTGAGGGACGTCCACACAGCACTGAGCACCCCAGGTAGCGGGCAGCTCCCAGGGACC
AATGACCTGGCCTCCACACCGGGCTCTGGCAGCAGCAGCATCTCAGCTGGGCTGCAGAAG
ATGGTGATTGAGAACGATCTTTCCGGTCTGATAGATTTCACCCGGTTACCGTCTCCAACC
CCCGAAAACAAGGACTTGTTTTTTGTCACAAGGTCCTCCGGGGTCCAGCCCTCACCTGCC
CGCAGCTCGAGTTACTCGGAAGCCAACGAGCCTGATCTTCAGATGGCCAACGGTGGCAAG
AGCCTCTCCATGGTGGACCTCCAGGACGCCCGCACGCTGGATGGGGAGGCAGGCTCCCCG
GCGGGCCCCGACGTCCTCCCCACAGATGGGCAGGCCGCTGCAGCTCAGCTGGTGGCCGGG
TGGCCGGCCCGGGCAACCCCAGTGAACCTGGCAGGGCTGGCCACGGTGCGGCGGGCAGGC
CAGACACCAACCACACCAGGCACCTCCGAGGGCGCGCCAGGCCGGCCCCAGCTGTTGGCA
CCGCTCTCCTTCCAGAACCCTGTGTACCAGATGGCGGCTGGCCTGCCGCTGTCACCCCGT
GGCCTTGGCGACTCAGGCTCTGAGGGCCACAGCTCCCTGAGCTCACACAGCAACAGCGAG
GAGTTGGCGGCTGCTGCCAAGCTGGGAAGTTTCAGCACTGCCGCGGAGGAGCTGGCTCGG
CGGCCCGGTGAGCTGGCACGGCGACAGATGTCACTGACTGAAAAAGGCGGGCAGCCCACG
GTGCCACGGCAGAACAGTGCTGGCCCCCAGAGGAGGATCGACCAGCCTCCGCCCCCACCC
CCGCCGCCACCTCCTGCCCCCCGCGGCCGGACGCCCCCCAACCTGCTGAGCACCCTGCAG
TACCCAAGACCCTCAAGCGGAACCCTGGCGTCGGCCTCACCTGATTGGGTGGGCCCCAGT
ACCCGCCTGAGGCAGCAGTCCTCTTCCTCCAAGGGGGACAGCCCAGAACTGAAGCCACGG
GCAGTGCACAAGCAGGGCCCTTCACCTGTGAGCCCCAATGCCCTGGACCGCACAGCCGCT
TGGCTCTTGACCATGAACGCGCAGTTGTTAGAAGACGAGGGCCTGGGCCCAGACCCCCCC
CACAGGGATAGGCTAAGGAGTAAGGACGAGCTCAGCCAAGCAGAAAAGGACCTGGCGGTG
CTGCAGGACAAGCTGCGAATCTCCACCAAGAAGCTGGAGGAGTATGAGACCCTGTTCAAG
TGCCAGGAGGAGACGACGCAGAAGCTGGTGCTGGAGTACCAGGCACGGCTGGAGGAGGGC
GAGGAGCGGCTGCGGCGGCAGCAGGAGGACAAGGACATCCAGATGAAGGGCATCATCAGC
AGGTTGATGTCCGTGGAGGAAGAACTGAAGAAGGACCACGCAGAGATGCAAGCGGCTGTG
GACTCCAAACAGAAGATCATTGATGCCCAGGAGAAGCGCATTGCCTCGTTGGATGCCGCC
AATGCCCGCCTCATGAGTGCCCTGACCCAGCTGAAAGAGAGTATGCAT
Protein sequence predicted based on nucleotide sequence
MPRLKESRSHESLLSPSSAVEALDLSMEEEVVIKPVHSSILGQDYCFEVTTSSGSKCFSC RSAAERDKWMENLRRAVHPNKDNSRRVEHILKLWVIEAKDLPAKKKYLCELCLDDVLYAR TTGKLKTDNVFWGEHFEFHNLPPLRTVTVHLYRETDKKKKKERNSYLGLVSLPAASVAGR QFVEKWYPVVTPNPKGGKGPGPMIRIKARYQTITILPMEMYKEFAEHITNHYLGLCAALE PILSAKTKEEMASALVHILQSTGKVKDFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEE YLKLVGQKYLQDALGEFIKALYESDENCEVDPSKCSAADLPEHQGNLKMCCELAFCKIIN SYCVFPRELKEVFASWRQECSSRGRPDISERLISASLFLRFLCPAIMSPSLFNLLQEYPD DRTARTLTLIAKVTQNLANFAKFGSKEEYMSFMNQFLEHEWTNMQRFLLEISNPETLSNT AGFEGYIDLGRELSSLHSLLWEAVSQLEQSIVSKLGPLPRILRDVHTALSTPGSGQLPGT NDLASTPGSGSSSISAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPA RSSSYSEANEPDLQMANGGKSLSMVDLQDARTLDGEAGSPAGPDVLPTDGQAAAAQLVAG WPARATPVNLAGLATVRRAGQTPTTPGTSEGAPGRPQLLAPLSFQNPVYQMAAGLPLSPR GLGDSGSEGHSSLSSHSNSEELAAAAKLGSFSTAAEELARRPGELARRQMSLTEKGGQPT VPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPNLLSTLQYPRPSSGTLASASPDWVGPS TRLRQQSSSSKGDSPELKPRAVHKQGPSPVSPNALDRTAAWLLTMNAQLLEDEGLGPDPP HRDRLRSKDELSQAEKDLAVLQDKLRISTKKLEEYETLFKCQEETTQKLVLEYQARLEEG EERLRRQQEDKDIQMKGIISRLMSVEEELKKDHAEMQAAVDSKQKIIDAQEKRIASLDAA NARLMSALTQLKESMH
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
