Gene:
Gene Symbol:
LRIG1
Gene Name:
leucine-rich repeats and immunoglobulin-like domains 1
Original Clone ID:
ccsbBroadEn_11799
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC071561.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516411
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGCGGCCGGTCCGGGGAGGGCTCGGGGCCCCGCGCCGCTCGCCTTGCCTTCTCCTT
CTCTGGCTGGTTTTGGTTCGGCTGGAGCCGGTGACCGCCGCGGCCGGCCCGCGGGCGCCC
TGCGCGGCCGCCTGCACTTGCGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCT
GCGTTGCCCGGGGACCTGCCCTCCTGGACGCGGAGCCTAAACCTGAGTTACAACAAATTC
TCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACCTACAGGAAGTGTACCTCAAT
AATAATGAGTTGACAGCGGTACCATCCCTGGGCGCTGCTTCATCACATGTCGTCTCTCTC
TTTCTGCAGCACAACAAGATTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCC
TTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCA
CACGGACCGCCTATAAAGGAGCTCAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTG
GGAGCATTTGATGGTCTGTCACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATC
ACCCAGCTTCCTGTAAGAGCATTCAAGCTACCCAGGCTGACACAACTGGACCTCAATCGG
AACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGCTCAACAGCTTGGAGGTGCTG
AAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGGACTGTCCAAG
ATGCATGTGCTGCACCTGGAGTACAACAGCCTGGTAGAAGTGAACAGCGGCTCGCTCTAC
GGCCTCACGGCCCTGCATCAGCTCCACCTCAGCAACAATTCCATCGCTCGCATTCACCGC
AAGGGCTGGAGCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACA
CGGCTGGACGAGGAGAGCCTGGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCAC
AATTCCATCAGCCACATTGCGGAGGGTGCCTTCAAGGGACTCAGGAGCCTGCGAGTCTTG
GATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACACGAGCGGCGCCTTCTCAGGG
CTCGACAGCCTCAGCAAGCTCCTTTTATTAGAGCCATCCCAGTCTGCTGGCTGCAGCTCC
CCGTCCCAGCCCCATATGTCTGCTGGGGGAAGGACTCTGTTTGGAAACAAGATCAAGTCT
GTGGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAAT
GCGATCAGATCTGTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCAT
ATCAGCAGCGACAGCTTCCTGTGTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATT
GGCAGGATGCTGCAGGCCTTTGTGACAGCCACCTGTGCCCACCCAGAATCACTGAAGGGT
CAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGATGACTTCCTGAAGCCACAG
ATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGTTTACATGC
TCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTC
CTGACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATG
GAGTACACCACCATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAA
TGTGTCATCACCAACCACTTTGGCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAAT
GTGTTGCCATCATTCACCAAAACGCCCCACGACATAACCATCCGGACCACCACCATGGCC
CGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATTGCCTGGCAGAAGGATGGA
GGCACGGATTTCCCCGCTGCCCAGACCCCATCCTTGGTGGTCCCCTTGGAAGACCGTGTG
GTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGC
ATCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACTTGACCCCT
GACAACCAGCTCCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGT
GAGATGTCCAACACCCTGGGCACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCA
GCAGGCTGCAGGAAGGATGGGACCACGGTAGGCATCTTCACCATTGCTGTCGTGAGCAGC
ATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCAGACCAGGAAGAAGAGTGAA
GAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCAAGCTACCTC
TCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCT
CAGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCA
GAGCCCGACACTCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTAT
CACAAAGAGCCGTGGAAAGCGATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATG
GAACACGGTGGCCGGGTCGTATGCAGTGACTGCAACACCGAAGTGGACTGTTACTCCAGG
GGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGCACAGCCAAGTGCGCCAAAT
GGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGCAGCAGGACT
GCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATG
CTGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGG
ACTTTAGCAAGGTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACT
TCAGGCAGTCCAGAGCGCGCGGAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCC
AAAGCATGTGACGCCAGTCCCGAGTCCACGCCACTGACAGGACAGCTCCCCGGGAAACAG
AGGGTGCCACTGCTGTTGGCACCAAAAAGC
Protein sequence predicted based on nucleotide sequence
MARPVRGGLGAPRRSPCLLLLWLVLVRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLA ALPGDLPSWTRSLNLSYNKFSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSL FLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLEL GAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVL KLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHR KGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVL DLDHNEISGTIEDTSGAFSGLDSLSKLLLLEPSQSAGCSSPSQPHMSAGGRTLFGNKIKS VAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLI GRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTC SAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQ CVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQIAWQKDG GTDFPAAQTPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTP DNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSS IVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGP QANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKM EHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRT AAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLT SGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
