DNASU Plasmid | THBS2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: THBS2

Gene Name: thrombospondin 2

Original Clone ID:

ccsbBroadEn_07065

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC150175.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00516420

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 3516nts

Open reading frame: 1 to 3516

Reference Sequence Alignment

ATGGCCTGGAGGCTGGTCCTGCTGGCTCTGTGGGTGTGGCCCAGCACGCAAGCTGGTCAC
CAGGACAAAGACACGACCTTCGACCTTTTCAGTATCAGCAACATCAACCGCAAGACCATT
GGCGCCAAGCAGTTCCGCGGGCCCGACCCCGGCGTGCCGGCTTACCGCTTCGTGCGCTTT
GACTACATCCCACCGGTGAACGCAGATGACCTCAGCAAGATCACCAAGATCATGCGGCAG
AAGGAGGGCTTCTTCCTCACGGCCCAGCTCAAGCAGGACGGCAAGTCCAGGGGCACGCTG
TTGGCTCTGGAGGGCCCCGGTCTCTCCCAGAGGCAGTTCGAGATCGTCTCCAACGGCCCC
GCGGACACGCTGGATCTCACCTACTGGATTGACGGCACCCGGCATGTGGTCTCCCTGGAG
GACGTCGGCCTGGCTGACTCGCAGTGGAAGAACGTCACCGTGCAGGTGGCTGGCGAGACC
TACAGCTTGCACGTGGGCTGCGACCTCATAGACAGCTTCGCTCTGGACGAGCCCTTCTAC
GAGCACCTGCAGGCGGAAAAGAGCCGGATGTACGTGGCCAAAGGCTCTGCCAGAGAGAGT
CACTTCAGGGGTTTGCTTCAGAACGTCCACCTAGTGTTTGAAAACTCTGTGGAAGATATT
CTAAGCAAGAAGGGTTGCCAGCAAGGCCAGGGAGCTGAGATCAACGCCATCAGTGAGAAC
ACAGAGACGCTGCGCCTGGGTCCGCATGTCACCACCGAGTACGTGGGCCCCAGCTCGGAG
AGGAGGCCCGAGGTGTGCGAACGCTCGTGCGAGGAGCTGGGAAACATGGTCCAGGAGCTC
TCGGGGCTCCACGTCCTCGTGAACCAGCTCAGCGAGAACCTCAAGAGAGTGTCGAATGAT
AACCAGTTTCTCTGGGAGCTCATTGGTGGCCCTCCTAAGACAAGGAACATGTCAGCTTGC
TGGCAGGATGGCCGGTTCTTTGCGGAAAATGAAACGTGGGTGGTGGACAGCTGCACCACG
TGTACCTGCAAGAAATTTAAAACCATTTGCCACCAAATCACCTGCCCGCCTGCAACCTGC
GCCAGTCCATCCTTTGTGGAAGGCGAATGCTGCCCTTCCTGCCTCCACTCGGTGGACGGT
GAGGAGGGCTGGTCTCCGTGGGCAGAGTGGACCCAGTGCTCCGTGACGTGTGGCTCTGGG
ACCCAGCAGAGAGGCCGGTCCTGTGACGTCACCAGCAACACCTGCTTGGGGCCCTCCATC
CAGACACGGGCTTGCAGTCTGAGCAAGTGTGACACCCGCATCCGGCAGGACGGCGGCTGG
AGCCACTGGTCACCTTGGTCTTCATGCTCTGTGACCTGTGGAGTTGGCAATATCACACGC
ATCCGTCTCTGCAACTCCCCAGTGCCCCAGATGGGGGGCAAGAATTGCAAAGGGAGTGGC
CGGGAGACCAAAGCCTGCCAGGGCGCCCCATGCCCAATCGATGGCCGCTGGAGCCCCTGG
TCCCCGTGGTCGGCCTGCACTGTCACCTGTGCCGGTGGGATCCGGGAGCGCACCCGGGTC
TGCAACAGCCCTGAGCCTCAGTACGGAGGGAAGGCCTGCGTGGGGGATGTGCAGGAGCGT
CAGATGTGCAACAAGAGGAGCTGCCCCGTGGATGGCTGTTTATCCAACCCCTGCTTCCCG
GGAGCCCAGTGCAGCAGCTTCCCCGATGGGTCCTGGTCATGCGGCTCCTGCCCTGTGGGC
TTCTTGGGCAATGGCACCCACTGTGAGGACCTGGACGAGTGTGCCCTGGTCCCCGACATC
TGCTTCTCCACCAGCAAGGTGCCTCGCTGTGTCAACACTCAGCCTGGCTTCCACTGCCTG
CCCTGCCCGCCCCGATACAGAGGGAACCAGCCCGTCGGGGTCGGCCTGGAAGCAGCCAAG
ACGGAAAAGCAAGTGTGTGAGCCCGAAAACCCATGCAAGGACAAGACACACAACTGCCAC
AAGCACGCGGAGTGCATCTACCTGGGCCACTTCAGCGACCCCATGTACAAGTGCGAGTGC
CAGACAGGCTACGCGGGCGACGGGCTCATCTGCGGGGAGGACTCGGACCTGGACGGCTGG
CCCAACCTCAATCTGGTCTGCGCCACCAACGCCACCTACCACTGCATCAAGGATAACTGC
CCCCATCTGCCAAATTCTGGGCAGGAAGACTTTGACAAGGACGGGATTGGCGATGCCTGT
GATGATGACGATGACAATGACGGTGTGACCGATGAGAAGGACAACTGCCAGCTCCTCTTC
AATCCCCGCCAGGCTGACTATGACAAGGATGAGGTTGGGGACCGCTGTGACAACTGCCCT
TACGTGCACAACCCTGCCCAGATCGACACAGACAACAATGGAGAGGGTGACGCCTGCTCC
GTGGACATTGATGGGGACGATGTCTTCAATGAACGAGACAATTGTCCCTACGTCTACAAC
ACTGACCAGAGGGACACGGATGGTGACGGTGTGGGGGATCACTGTGACAACTGCCCCCTG
GTGCACAACCCTGACCAGACCGACGTGGACAATGACCTTGTTGGGGACCAGTGTGACAAC
AACGAGGACATAGATGACGACGGCCACCAGAACAACCAGGACAACTGCCCCTACATCTCC
AACGCCAACCAGGCTGACCATGACAGAGACGGCCAGGGCGACGCCTGTGACCCTGATGAT
GACAACGATGGCGTCCCCGATGACAGGGACAACTGCCGGCTTGTGTTCAACCCAGACCAG
GAGGACTTGGACGGTGATGGACGGGGTGATATTTGTAAAGATGATTTTGACAATGACAAC
ATCCCAGATATTGATGATGTGTGTCCTGAAAACAATGCCATCAGTGAGACAGACTTCAGG
AACTTCCAGATGGTCCCCTTGGATCCCAAAGGGACCACCCAAATTGATCCCAACTGGGTC
ATTCGCCATCAAGGCAAGGAGCTGGTTCAGACAGCCAACTCGGACCCCGGCATCGCTGTA
GGTTTTGACGAGTTTGGGTCTGTGGACTTCAGTGGCACATTCTACGTAAACACTGACCGG
GACGACGACTATGCCGGCTTCGTCTTTGGTTACCAGTCAAGCAGCCGCTTCTATGTGGTG
ATGTGGAAGCAGGTGACGCAGACCTACTGGGAGGACCAGCCCACGCGGGCCTATGGCTAC
TCCGGCGTGTCCCTCAAGGTGGTGAACTCCACCACGGGGACGGGCGAGCACCTGAGGAAC
GCGCTGTGGCACACGGGGAACACGCCGGGGCAGGTGCGAACCTTATGGCACGACCCCAGG
AACATTGGCTGGAAGGACTACACGGCCTATAGGTGGCACCTGACTCACAGGCCCAAGACT
GGCTACATCAGAGTCTTAGTGCATGAAGGAAAACAGGTCATGGCAGACTCAGGACCTATC
TATGACCAAACCTACGCTGGCGGGCGGCTGGGTCTATTTGTCTTCTCTCAAGAAATGGTC
TATTTCTCAGACCTCAAGTACGAATGCAGAGATATT

Protein sequence predicted based on nucleotide sequence

MAWRLVLLALWVWPSTQAGHQDKDTTFDLFSISNINRKTIGAKQFRGPDPGVPAYRFVRF
DYIPPVNADDLSKITKIMRQKEGFFLTAQLKQDGKSRGTLLALEGPGLSQRQFEIVSNGP
ADTLDLTYWIDGTRHVVSLEDVGLADSQWKNVTVQVAGETYSLHVGCDLIDSFALDEPFY
EHLQAEKSRMYVAKGSARESHFRGLLQNVHLVFENSVEDILSKKGCQQGQGAEINAISEN
TETLRLGPHVTTEYVGPSSERRPEVCERSCEELGNMVQELSGLHVLVNQLSENLKRVSND
NQFLWELIGGPPKTRNMSACWQDGRFFAENETWVVDSCTTCTCKKFKTICHQITCPPATC
ASPSFVEGECCPSCLHSVDGEEGWSPWAEWTQCSVTCGSGTQQRGRSCDVTSNTCLGPSI
QTRACSLSKCDTRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPVPQMGGKNCKGSG
RETKACQGAPCPIDGRWSPWSPWSACTVTCAGGIRERTRVCNSPEPQYGGKACVGDVQER
QMCNKRSCPVDGCLSNPCFPGAQCSSFPDGSWSCGSCPVGFLGNGTHCEDLDECALVPDI
CFSTSKVPRCVNTQPGFHCLPCPPRYRGNQPVGVGLEAAKTEKQVCEPENPCKDKTHNCH
KHAECIYLGHFSDPMYKCECQTGYAGDGLICGEDSDLDGWPNLNLVCATNATYHCIKDNC
PHLPNSGQEDFDKDGIGDACDDDDDNDGVTDEKDNCQLLFNPRQADYDKDEVGDRCDNCP
YVHNPAQIDTDNNGEGDACSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPL
VHNPDQTDVDNDLVGDQCDNNEDIDDDGHQNNQDNCPYISNANQADHDRDGQGDACDPDD
DNDGVPDDRDNCRLVFNPDQEDLDGDGRGDICKDDFDNDNIPDIDDVCPENNAISETDFR
NFQMVPLDPKGTTQIDPNWVIRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDR
DDDYAGFVFGYQSSSRFYVVMWKQVTQTYWEDQPTRAYGYSGVSLKVVNSTTGTGEHLRN
ALWHTGNTPGQVRTLWHDPRNIGWKDYTAYRWHLTHRPKTGYIRVLVHEGKQVMADSGPI
YDQTYAGGRLGLFVFSQEMVYFSDLKYECRDI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

THBS2

Symbol Nomenclature :

THBS2

SYNONYM :

TSP2

Designation :

thrombospondin-2

Full Nomenclature :

thrombospondin 2

GENEID :

7058

Locus Tag :

XXyac-YX65C7_A.1

HGNC :

11786

MIM :

188061

Vega :

OTTHUMG00000045408

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00516420      ATGGCCTGGAGGCTGGTCCTGCTGGCTCTGTGGGTGTGGCCCAGCACGCAAGCTGGTCAC
NM_003247.3       ATGGTCTGGAGGCTGGTCCTGCTGGCTCTGTGGGTGTGGCCCAGCACGCAAGCTGGTCAC
                  **** *******************************************************

HsCD00516420      CAGGACAAAGACACGACCTTCGACCTTTTCAGTATCAGCAACATCAACCGCAAGACCATT
NM_003247.3       CAGGACAAAGACACGACCTTCGACCTTTTCAGTATCAGCAACATCAACCGCAAGACCATT
                  ************************************************************

HsCD00516420      GGCGCCAAGCAGTTCCGCGGGCCCGACCCCGGCGTGCCGGCTTACCGCTTCGTGCGCTTT
NM_003247.3       GGCGCCAAGCAGTTCCGCGGGCCCGACCCCGGCGTGCCGGCTTACCGCTTCGTGCGCTTT
                  ************************************************************

HsCD00516420      GACTACATCCCACCGGTGAACGCAGATGACCTCAGCAAGATCACCAAGATCATGCGGCAG
NM_003247.3       GACTACATCCCACCGGTGAACGCAGATGACCTCAGCAAGATCACCAAGATCATGCGGCAG
                  ************************************************************

HsCD00516420      AAGGAGGGCTTCTTCCTCACGGCCCAGCTCAAGCAGGACGGCAAGTCCAGGGGCACGCTG
NM_003247.3       AAGGAGGGCTTCTTCCTCACGGCCCAGCTCAAGCAGGACGGCAAGTCCAGGGGCACGCTG
                  ************************************************************

HsCD00516420      TTGGCTCTGGAGGGCCCCGGTCTCTCCCAGAGGCAGTTCGAGATCGTCTCCAACGGCCCC
NM_003247.3       TTGGCTCTGGAGGGCCCCGGTCTCTCCCAGAGGCAGTTCGAGATCGTCTCCAACGGCCCC
                  ************************************************************

HsCD00516420      GCGGACACGCTGGATCTCACCTACTGGATTGACGGCACCCGGCATGTGGTCTCCCTGGAG
NM_003247.3       GCGGACACGCTGGATCTCACCTACTGGATTGACGGCACCCGGCATGTGGTCTCCCTGGAG
                  ************************************************************

HsCD00516420      GACGTCGGCCTGGCTGACTCGCAGTGGAAGAACGTCACCGTGCAGGTGGCTGGCGAGACC
NM_003247.3       GACGTCGGCCTGGCTGACTCGCAGTGGAAGAACGTCACCGTGCAGGTGGCTGGCGAGACC
                  ************************************************************

HsCD00516420      TACAGCTTGCACGTGGGCTGCGACCTCATAGACAGCTTCGCTCTGGACGAGCCCTTCTAC
NM_003247.3       TACAGCTTGCACGTGGGCTGCGACCTCATAGACAGCTTCGCTCTGGACGAGCCCTTCTAC
                  ************************************************************

HsCD00516420      GAGCACCTGCAGGCGGAAAAGAGCCGGATGTACGTGGCCAAAGGCTCTGCCAGAGAGAGT
NM_003247.3       GAGCACCTGCAGGCGGAAAAGAGCCGGATGTACGTGGCCAAAGGCTCTGCCAGAGAGAGT
                  ************************************************************

HsCD00516420      CACTTCAGGGGTTTGCTTCAGAACGTCCACCTAGTGTTTGAAAACTCTGTGGAAGATATT
NM_003247.3       CACTTCAGGGGTTTGCTTCAGAACGTCCACCTAGTGTTTGAAAACTCTGTGGAAGATATT
                  ************************************************************

HsCD00516420      CTAAGCAAGAAGGGTTGCCAGCAAGGCCAGGGAGCTGAGATCAACGCCATCAGTGAGAAC
NM_003247.3       CTAAGCAAGAAGGGTTGCCAGCAAGGCCAGGGAGCTGAGATCAACGCCATCAGTGAGAAC
                  ************************************************************

HsCD00516420      ACAGAGACGCTGCGCCTGGGTCCGCATGTCACCACCGAGTACGTGGGCCCCAGCTCGGAG
NM_003247.3       ACAGAGACGCTGCGCCTGGGTCCGCATGTCACCACCGAGTACGTGGGCCCCAGCTCGGAG
                  ************************************************************

HsCD00516420      AGGAGGCCCGAGGTGTGCGAACGCTCGTGCGAGGAGCTGGGAAACATGGTCCAGGAGCTC
NM_003247.3       AGGAGGCCCGAGGTGTGCGAACGCTCGTGCGAGGAGCTGGGAAACATGGTCCAGGAGCTC
                  ************************************************************

HsCD00516420      TCGGGGCTCCACGTCCTCGTGAACCAGCTCAGCGAGAACCTCAAGAGAGTGTCGAATGAT
NM_003247.3       TCGGGGCTCCACGTCCTCGTGAACCAGCTCAGCGAGAACCTCAAGAGAGTGTCGAATGAT
                  ************************************************************

HsCD00516420      AACCAGTTTCTCTGGGAGCTCATTGGTGGCCCTCCTAAGACAAGGAACATGTCAGCTTGC
NM_003247.3       AACCAGTTTCTCTGGGAGCTCATTGGTGGCCCTCCTAAGACAAGGAACATGTCAGCTTGC
                  ************************************************************

HsCD00516420      TGGCAGGATGGCCGGTTCTTTGCGGAAAATGAAACGTGGGTGGTGGACAGCTGCACCACG
NM_003247.3       TGGCAGGATGGCCGGTTCTTTGCGGAAAATGAAACGTGGGTGGTGGACAGCTGCACCACG
                  ************************************************************

HsCD00516420      TGTACCTGCAAGAAATTTAAAACCATTTGCCACCAAATCACCTGCCCGCCTGCAACCTGC
NM_003247.3       TGTACCTGCAAGAAATTTAAAACCATTTGCCACCAAATCACCTGCCCGCCTGCAACCTGC
                  ************************************************************

HsCD00516420      GCCAGTCCATCCTTTGTGGAAGGCGAATGCTGCCCTTCCTGCCTCCACTCGGTGGACGGT
NM_003247.3       GCCAGTCCATCCTTTGTGGAAGGCGAATGCTGCCCTTCCTGCCTCCACTCGGTGGACGGT
                  ************************************************************

HsCD00516420      GAGGAGGGCTGGTCTCCGTGGGCAGAGTGGACCCAGTGCTCCGTGACGTGTGGCTCTGGG
NM_003247.3       GAGGAGGGCTGGTCTCCGTGGGCAGAGTGGACCCAGTGCTCCGTGACGTGTGGCTCTGGG
                  ************************************************************

HsCD00516420      ACCCAGCAGAGAGGCCGGTCCTGTGACGTCACCAGCAACACCTGCTTGGGGCCCTCCATC
NM_003247.3       ACCCAGCAGAGAGGCCGGTCCTGTGACGTCACCAGCAACACCTGCTTGGGGCCCTCCATC
                  ************************************************************

HsCD00516420      CAGACACGGGCTTGCAGTCTGAGCAAGTGTGACACCCGCATCCGGCAGGACGGCGGCTGG
NM_003247.3       CAGACACGGGCTTGCAGTCTGAGCAAGTGTGACACCCGCATCCGGCAGGACGGCGGCTGG
                  ************************************************************

HsCD00516420      AGCCACTGGTCACCTTGGTCTTCATGCTCTGTGACCTGTGGAGTTGGCAATATCACACGC
NM_003247.3       AGCCACTGGTCACCTTGGTCTTCATGCTCTGTGACCTGTGGAGTTGGCAATATCACACGC
                  ************************************************************

HsCD00516420      ATCCGTCTCTGCAACTCCCCAGTGCCCCAGATGGGGGGCAAGAATTGCAAAGGGAGTGGC
NM_003247.3       ATCCGTCTCTGCAACTCCCCAGTGCCCCAGATGGGGGGCAAGAATTGCAAAGGGAGTGGC
                  ************************************************************

HsCD00516420      CGGGAGACCAAAGCCTGCCAGGGCGCCCCATGCCCAATCGATGGCCGCTGGAGCCCCTGG
NM_003247.3       CGGGAGACCAAAGCCTGCCAGGGCGCCCCATGCCCAATCGATGGCCGCTGGAGCCCCTGG
                  ************************************************************

HsCD00516420      TCCCCGTGGTCGGCCTGCACTGTCACCTGTGCCGGTGGGATCCGGGAGCGCACCCGGGTC
NM_003247.3       TCCCCGTGGTCGGCCTGCACTGTCACCTGTGCCGGTGGGATCCGGGAGCGCACCCGGGTC
                  ************************************************************

HsCD00516420      TGCAACAGCCCTGAGCCTCAGTACGGAGGGAAGGCCTGCGTGGGGGATGTGCAGGAGCGT
NM_003247.3       TGCAACAGCCCTGAGCCTCAGTACGGAGGGAAGGCCTGCGTGGGGGATGTGCAGGAGCGT
                  ************************************************************

HsCD00516420      CAGATGTGCAACAAGAGGAGCTGCCCCGTGGATGGCTGTTTATCCAACCCCTGCTTCCCG
NM_003247.3       CAGATGTGCAACAAGAGGAGCTGCCCCGTGGATGGCTGTTTATCCAACCCCTGCTTCCCG
                  ************************************************************

HsCD00516420      GGAGCCCAGTGCAGCAGCTTCCCCGATGGGTCCTGGTCATGCGGCTCCTGCCCTGTGGGC
NM_003247.3       GGAGCCCAGTGCAGCAGCTTCCCCGATGGGTCCTGGTCATGCGGCTCCTGCCCTGTGGGC
                  ************************************************************

HsCD00516420      TTCTTGGGCAATGGCACCCACTGTGAGGACCTGGACGAGTGTGCCCTGGTCCCCGACATC
NM_003247.3       TTCTTGGGCAATGGCACCCACTGTGAGGACCTGGACGAGTGTGCCCTGGTCCCCGACATC
                  ************************************************************

HsCD00516420      TGCTTCTCCACCAGCAAGGTGCCTCGCTGTGTCAACACTCAGCCTGGCTTCCACTGCCTG
NM_003247.3       TGCTTCTCCACCAGCAAGGTGCCTCGCTGTGTCAACACTCAGCCTGGCTTCCACTGCCTG
                  ************************************************************

HsCD00516420      CCCTGCCCGCCCCGATACAGAGGGAACCAGCCCGTCGGGGTCGGCCTGGAAGCAGCCAAG
NM_003247.3       CCCTGCCCGCCCCGATACAGAGGGAACCAGCCCGTCGGGGTCGGCCTGGAAGCAGCCAAG
                  ************************************************************

HsCD00516420      ACGGAAAAGCAAGTGTGTGAGCCCGAAAACCCATGCAAGGACAAGACACACAACTGCCAC
NM_003247.3       ACGGAAAAGCAAGTGTGTGAGCCCGAAAACCCATGCAAGGACAAGACACACAACTGCCAC
                  ************************************************************

HsCD00516420      AAGCACGCGGAGTGCATCTACCTGGGCCACTTCAGCGACCCCATGTACAAGTGCGAGTGC
NM_003247.3       AAGCACGCGGAGTGCATCTACCTGGGCCACTTCAGCGACCCCATGTACAAGTGCGAGTGC
                  ************************************************************

HsCD00516420      CAGACAGGCTACGCGGGCGACGGGCTCATCTGCGGGGAGGACTCGGACCTGGACGGCTGG
NM_003247.3       CAGACAGGCTACGCGGGCGACGGGCTCATCTGCGGGGAGGACTCGGACCTGGACGGCTGG
                  ************************************************************

HsCD00516420      CCCAACCTCAATCTGGTCTGCGCCACCAACGCCACCTACCACTGCATCAAGGATAACTGC
NM_003247.3       CCCAACCTCAATCTGGTCTGCGCCACCAACGCCACCTACCACTGCATCAAGGATAACTGC
                  ************************************************************

HsCD00516420      CCCCATCTGCCAAATTCTGGGCAGGAAGACTTTGACAAGGACGGGATTGGCGATGCCTGT
NM_003247.3       CCCCATCTGCCAAATTCTGGGCAGGAAGACTTTGACAAGGACGGGATTGGCGATGCCTGT
                  ************************************************************

HsCD00516420      GATGATGACGATGACAATGACGGTGTGACCGATGAGAAGGACAACTGCCAGCTCCTCTTC
NM_003247.3       GATGATGACGATGACAATGACGGTGTGACCGATGAGAAGGACAACTGCCAGCTCCTCTTC
                  ************************************************************

HsCD00516420      AATCCCCGCCAGGCTGACTATGACAAGGATGAGGTTGGGGACCGCTGTGACAACTGCCCT
NM_003247.3       AATCCCCGCCAGGCTGACTATGACAAGGATGAGGTTGGGGACCGCTGTGACAACTGCCCT
                  ************************************************************

HsCD00516420      TACGTGCACAACCCTGCCCAGATCGACACAGACAACAATGGAGAGGGTGACGCCTGCTCC
NM_003247.3       TACGTGCACAACCCTGCCCAGATCGACACAGACAACAATGGAGAGGGTGACGCCTGCTCC
                  ************************************************************

HsCD00516420      GTGGACATTGATGGGGACGATGTCTTCAATGAACGAGACAATTGTCCCTACGTCTACAAC
NM_003247.3       GTGGACATTGATGGGGACGATGTCTTCAATGAACGAGACAATTGTCCCTACGTCTACAAC
                  ************************************************************

HsCD00516420      ACTGACCAGAGGGACACGGATGGTGACGGTGTGGGGGATCACTGTGACAACTGCCCCCTG
NM_003247.3       ACTGACCAGAGGGACACGGATGGTGACGGTGTGGGGGATCACTGTGACAACTGCCCCCTG
                  ************************************************************

HsCD00516420      GTGCACAACCCTGACCAGACCGACGTGGACAATGACCTTGTTGGGGACCAGTGTGACAAC
NM_003247.3       GTGCACAACCCTGACCAGACCGACGTGGACAATGACCTTGTTGGGGACCAGTGTGACAAC
                  ************************************************************

HsCD00516420      AACGAGGACATAGATGACGACGGCCACCAGAACAACCAGGACAACTGCCCCTACATCTCC
NM_003247.3       AACGAGGACATAGATGACGACGGCCACCAGAACAACCAGGACAACTGCCCCTACATCTCC
                  ************************************************************

HsCD00516420      AACGCCAACCAGGCTGACCATGACAGAGACGGCCAGGGCGACGCCTGTGACCCTGATGAT
NM_003247.3       AACGCCAACCAGGCTGACCATGACAGAGACGGCCAGGGCGACGCCTGTGACCCTGATGAT
                  ************************************************************

HsCD00516420      GACAACGATGGCGTCCCCGATGACAGGGACAACTGCCGGCTTGTGTTCAACCCAGACCAG
NM_003247.3       GACAACGATGGCGTCCCCGATGACAGGGACAACTGCCGGCTTGTGTTCAACCCAGACCAG
                  ************************************************************

HsCD00516420      GAGGACTTGGACGGTGATGGACGGGGTGATATTTGTAAAGATGATTTTGACAATGACAAC
NM_003247.3       GAGGACTTGGACGGTGATGGACGGGGTGATATTTGTAAAGATGATTTTGACAATGACAAC
                  ************************************************************

HsCD00516420      ATCCCAGATATTGATGATGTGTGTCCTGAAAACAATGCCATCAGTGAGACAGACTTCAGG
NM_003247.3       ATCCCAGATATTGATGATGTGTGTCCTGAAAACAATGCCATCAGTGAGACAGACTTCAGG
                  ************************************************************

HsCD00516420      AACTTCCAGATGGTCCCCTTGGATCCCAAAGGGACCACCCAAATTGATCCCAACTGGGTC
NM_003247.3       AACTTCCAGATGGTCCCCTTGGATCCCAAAGGGACCACCCAAATTGATCCCAACTGGGTC
                  ************************************************************

HsCD00516420      ATTCGCCATCAAGGCAAGGAGCTGGTTCAGACAGCCAACTCGGACCCCGGCATCGCTGTA
NM_003247.3       ATTCGCCATCAAGGCAAGGAGCTGGTTCAGACAGCCAACTCGGACCCCGGCATCGCTGTA
                  ************************************************************

HsCD00516420      GGTTTTGACGAGTTTGGGTCTGTGGACTTCAGTGGCACATTCTACGTAAACACTGACCGG
NM_003247.3       GGTTTTGACGAGTTTGGGTCTGTGGACTTCAGTGGCACATTCTACGTAAACACTGACCGG
                  ************************************************************

HsCD00516420      GACGACGACTATGCCGGCTTCGTCTTTGGTTACCAGTCAAGCAGCCGCTTCTATGTGGTG
NM_003247.3       GACGACGACTATGCCGGCTTCGTCTTTGGTTACCAGTCAAGCAGCCGCTTCTATGTGGTG
                  ************************************************************

HsCD00516420      ATGTGGAAGCAGGTGACGCAGACCTACTGGGAGGACCAGCCCACGCGGGCCTATGGCTAC
NM_003247.3       ATGTGGAAGCAGGTGACGCAGACCTACTGGGAGGACCAGCCCACGCGGGCCTATGGCTAC
                  ************************************************************

HsCD00516420      TCCGGCGTGTCCCTCAAGGTGGTGAACTCCACCACGGGGACGGGCGAGCACCTGAGGAAC
NM_003247.3       TCCGGCGTGTCCCTCAAGGTGGTGAACTCCACCACGGGGACGGGCGAGCACCTGAGGAAC
                  ************************************************************

HsCD00516420      GCGCTGTGGCACACGGGGAACACGCCGGGGCAGGTGCGAACCTTATGGCACGACCCCAGG
NM_003247.3       GCGCTGTGGCACACGGGGAACACGCCGGGGCAGGTGCGAACCTTATGGCACGACCCCAGG
                  ************************************************************

HsCD00516420      AACATTGGCTGGAAGGACTACACGGCCTATAGGTGGCACCTGACTCACAGGCCCAAGACT
NM_003247.3       AACATTGGCTGGAAGGACTACACGGCCTATAGGTGGCACCTGACTCACAGGCCCAAGACT
                  ************************************************************

HsCD00516420      GGCTACATCAGAGTCTTAGTGCATGAAGGAAAACAGGTCATGGCAGACTCAGGACCTATC
NM_003247.3       GGCTACATCAGAGTCTTAGTGCATGAAGGAAAACAGGTCATGGCAGACTCAGGACCTATC
                  ************************************************************

HsCD00516420      TATGACCAAACCTACGCTGGCGGGCGGCTGGGTCTATTTGTCTTCTCTCAAGAAATGGTC
NM_003247.3       TATGACCAAACCTACGCTGGCGGGCGGCTGGGTCTATTTGTCTTCTCTCAAGAAATGGTC
                  ************************************************************

HsCD00516420      TATTTCTCAGACCTCAAGTACGAATGCAGAGATATT---
NM_003247.3       TATTTCTCAGACCTCAAGTACGAATGCAGAGATATTTAA
                  ************************************