PCDH12 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PCDH12
Gene Name:  protocadherin 12
Original Clone ID:
ccsbBroadEn_10458
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC042634.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516424
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3561nts
Open reading frame: 1 to 3561

ATGATGCAACTTCTGCAACTTCTGCTGGGGCTTTTGGGGCCAGGTGGCTACTTATTTCTT
TTAGGGGATTGTCAGGAGGTGACCACTCTCACGGTGAAATACCAAGTGTCAGAGGAAGTG
CCATCTGGTACAGTGATCGGGAAGCTGTCCCAGGAACTGGGCCGGGAGGAGAGGCGGAGG
CAAGCTGGGGCCGCCTTCCAGGTGTTGCAGCTGCCTCAGGCGCTCCCCATTCAGGTGGAC
TCTGAGGAAGGCTTGCTCAGCACAGGCAGGCGGCTGGATCGAGAGCAGCTATGCCGACAG
TGGGATCCCTGCCTGGTTTCCTTTGATGTGCTTGCCACAGGGGATTTGGCTCTGATCCAT
GTGGAGATCCAAGTGCTGGACATCAATGACCACCAGCCACGGTTTCCCAAAGGCGAGCAG
GAGCTGGAAATCTCTGAGAGCGCCTCTCTGCGAACCCGGATCCCCCTGGACAGAGCTCTT
GACCCAGACACAGGCCCTAACACCCTGCACACCTACACTCTGTCTCCCAGTGAGCACTTT
GCCTTGGATGTCATTGTGGGCCCTGATGAGACCAAACATGCAGAACTCATAGTGGTGAAG
GAGCTGGACAGGGAAATCCATTCATTTTTTGATCTGGTGTTAACTGCCTATGACAATGGG
AACCCCCCCAAGTCAGGTACCAGCTTGGTCAAGGTCAACGTCTTGGACTCCAATGACAAT
AGCCCTGCGTTTGCTGAGAGTTCACTGGCACTGGAAATCCAAGAAGATGCTGCACCTGGT
ACGCTTCTCATAAAACTGACCGCCACAGACCCTGACCAAGGCCCCAATGGGGAGGTGGAG
TTCTTCCTCAGTAAGCACATGCCTCCAGAGGTGCTGGACACCTTCAGTATTGATGCCAAG
ACAGGCCAGGTCATTCTGCGTCGACCTCTAGACTATGAAAAGAACCCTGCCTACGAGGTG
GATGTTCAGGCAAGGGACCTGGGTCCCAATCCTATCCCAGCCCATTGCAAAGTTCTCATC
AAGGTTCTGGATGTCAATGACAACATCCCAAGCATCCACGTCACATGGGCCTCCCAGCCA
TCACTGGTGTCAGAAGCTCTTCCCAAGGACAGTTTTATTGCTCTTGTCATGGCAGATGAC
TTGGATTCAGGAAACAATGGTTTGGTCCACTGCTGGCTGAGCCAAGAGCTGGGCCACTTC
AGGCTGAAAAGAACTAATGGCAACACATACATGTTGCTAACCAATGCCACACTGGACAGA
GAGCAGTGGCCCAAATATACCCTCACTCTGTTAGCCCAAGACCAAGGACTCCAGCCCTTA
TCAGCCAAGAAACAGCTCAGCATTCAGATCAGTGACATCAACGACAATGCACCTGTGTTT
GAGAAAAGCAGGTATGAAGTCTCCACGCGGGAAAACAACTTACCCTCTCTTCACCTCATT
ACCATCAAGGCTCATGATGCAGACTTGGGCATTAATGGAAAAGTCTCATACCGCATCCAG
GACTCCCCAGTTGCTCACTTAGTAGCTATTGACTCCAACACAGGAGAGGTCACTGCTCAG
AGGTCACTGAACTATGAAGAGATGGCCGGCTTTGAGTTCCAGGTGATCGCAGAGGACAGC
GGGCAACCCATGCTTGCATCCAGTGTCTCTGTGTGGGTCAGCCTCTTGGATGCCAATGAT
AATGCCCCAGAGGTGGTCCAGCCTGTGCTCAGCGATGGAAAAGCCAGCCTCTCCGTGCTT
GTGAATGCCTCCACAGGCCACCTGCTGGTGCCCATCGAGACTCCCAATGGCTTGGGCCCA
GCGGGCACTGACACACCTCCACTGGCCACTCACAGCTCCCGGCCATTCCTTTTGACAACC
ATTGTGGCAAGAGATGCAGACTCGGGGGCAAATGGAGAGCCCCTCTACAGCATCCGCAGT
GGAAATGAAGCCCACCTCTTCATCCTCAACCCTCATACGGGGCAGCTGTTCGTCAATGTC
ACCAATGCCAGCAGCCTCATTGGGAGTGAGTGGGAGCTGGAGATAGTAGTAGAGGACCAG
GGAAGCCCCCCCTTACAGACCCGAGCCCTGTTGAGGGTCATGTTTGTCACCAGTGTGGAC
CACCTGAGGGACTCAGCCCGCAAGCCTGGGGCTTTGAGCATGTCGATGCTGACGGTGATC
TGCCTGGCTGTACTGCTGGGCATCTTCGGGTTGATCCTGGCTTTGTTCATGTCCATCTGC
CGGACAGAAAAGAAGGACAACAGGGCCTACAACTGTCGGGAGGCCGAGTCCGCCTACCGC
CAGCAGCCCAAGAGGCCCCAGAAACACATTCAGAAGGCAGACATCCACCTCGTGCCTGTG
CTCAGGGGTCAGGCAGGTGAGCCTTGTGAAGTCGGGCAGTCCCACAAAGATGTGGACAAG
GAGGCGATGATGGAAGCAGGCTGGGACCCCTGCCTGCAGGCCCCCTTCCACCTCACCCCG
ACCCTGTACAGGACGCTGCGTAATCAAGGCAACCAGGGAGCACCGGCGGAGAGCCGAGAG
GTGCTGCAAGACACGGTCAACCTCCTTTTCAACCATCCCAGGCAGAGGAATGCCTCCCGG
GAGAACCTGAACCTTCCCGAGCCCCAGCCTGCCACAGGCCAGCCACGTTCCAGGCCTCTG
AAGGTTGCAGGCAGCCCCACAGGGAGGCTGGCTGGAGACCAGGGCAGTGAGGAAGCCCCA
CAGAGGCCACCAGCCTCCTCTGCAACCCTGAGACGGCAGCGACATCTCAATGGCAAAGTG
TCCCCTGAGAAAGAATCAGGGCCCCGTCAGATCCTGCGGAGCCTGGTCCGGCTGTCTGTG
GCTGCCTTCGCCGAGCGGAACCCCGTGGAGGAGCTCACTGTGGATTCTCCTCCTGTTCAG
CAAATCTCCCAGCTGCTGTCCTTGCTGCATCAGGGCCAATTCCAGCCCAAACCAAACCAC
CGAGGAAATAAGTACTTGGCCAAGCCAGGAGGCAGCAGGAGTGCAATCCCAGACACAGAT
GGCCCAAGTGCAAGGGCTGGAGGCCAGACAGACCCAGAACAGGAGGAAGGGCCTTTGGAT
CCTGAAGAGGACCTCTCTGTGAAGCAACTGCTAGAAGAAGAGCTGTCAAGTCTGCTGGAC
CCCAGCACAGGTCTGGCCCTGGACCGGCTGAGCGCCCCTGACCCGGCCTGGATGGCGAGA
CTCTCTTTGCCCCTCACCACCAACTACCGTGACAATGTGATCTCCCCGGATGCTGCAGCC
ACGGAGGAGCCGAGGACCTTCCAGACGTTCGGCAAGGCAGAGGCACCAGAGCTGAGCCCA
ACAGGCACGAGGCTGGCCAGCACCTTTGTCTCGGAGATGAGCTCACTGCTGGAGATGCTG
CTGGAACAGCGCTCCAGCATGCCCGTGGAGGCCGCCTCCGAGGCGCTGCGGCGGCTCTCG
GTCTGCGGGAGGACCCTCAGTTTAGACTTGGCCACCAGTGCAGCCTCAGGCATGAAAGTG
CAAGGGGACCCAGGTGGAAAGACGGGGACTGAGGGCAAGAGCAGAGGCAGCAGCAGCAGC
AGCAGCAGCAGCAGGTGCCTG

Protein sequence predicted based on nucleotide sequence

MMQLLQLLLGLLGPGGYLFLLGDCQEVTTLTVKYQVSEEVPSGTVIGKLSQELGREERRR
QAGAAFQVLQLPQALPIQVDSEEGLLSTGRRLDREQLCRQWDPCLVSFDVLATGDLALIH
VEIQVLDINDHQPRFPKGEQELEISESASLRTRIPLDRALDPDTGPNTLHTYTLSPSEHF
ALDVIVGPDETKHAELIVVKELDREIHSFFDLVLTAYDNGNPPKSGTSLVKVNVLDSNDN
SPAFAESSLALEIQEDAAPGTLLIKLTATDPDQGPNGEVEFFLSKHMPPEVLDTFSIDAK
TGQVILRRPLDYEKNPAYEVDVQARDLGPNPIPAHCKVLIKVLDVNDNIPSIHVTWASQP
SLVSEALPKDSFIALVMADDLDSGNNGLVHCWLSQELGHFRLKRTNGNTYMLLTNATLDR
EQWPKYTLTLLAQDQGLQPLSAKKQLSIQISDINDNAPVFEKSRYEVSTRENNLPSLHLI
TIKAHDADLGINGKVSYRIQDSPVAHLVAIDSNTGEVTAQRSLNYEEMAGFEFQVIAEDS
GQPMLASSVSVWVSLLDANDNAPEVVQPVLSDGKASLSVLVNASTGHLLVPIETPNGLGP
AGTDTPPLATHSSRPFLLTTIVARDADSGANGEPLYSIRSGNEAHLFILNPHTGQLFVNV
TNASSLIGSEWELEIVVEDQGSPPLQTRALLRVMFVTSVDHLRDSARKPGALSMSMLTVI
CLAVLLGIFGLILALFMSICRTEKKDNRAYNCREAESAYRQQPKRPQKHIQKADIHLVPV
LRGQAGEPCEVGQSHKDVDKEAMMEAGWDPCLQAPFHLTPTLYRTLRNQGNQGAPAESRE
VLQDTVNLLFNHPRQRNASRENLNLPEPQPATGQPRSRPLKVAGSPTGRLAGDQGSEEAP
QRPPASSATLRRQRHLNGKVSPEKESGPRQILRSLVRLSVAAFAERNPVEELTVDSPPVQ
QISQLLSLLHQGQFQPKPNHRGNKYLAKPGGSRSAIPDTDGPSARAGGQTDPEQEEGPLD
PEEDLSVKQLLEEELSSLLDPSTGLALDRLSAPDPAWMARLSLPLTTNYRDNVISPDAAA
TEEPRTFQTFGKAEAPELSPTGTRLASTFVSEMSSLLEMLLEQRSSMPVEAASEALRRLS
VCGRTLSLDLATSAASGMKVQGDPGGKTGTEGKSRGSSSSSSSSRCL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable