Gene:
Gene Symbol:
CHL1
Gene Name:
cell adhesion molecule L1-like
Original Clone ID:
ccsbBroadEn_11544
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC104918.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516427
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGCCGCTTTTACTTGGAAGAGGACTAATCGTATATCTAATGTTCCTCCTGTTAAAA
TTCTCAAAAGCAATTGAAATACCATCTTCAGTTCAACAGGTTCCAACAATCATAAAACAG
TCAAAAGTCCAAGTTGCCTTTCCCTTCGATGAGTATTTTCAAATTGAATGTGAAGCTAAA
GGAAATCCAGAACCAACATTTTCGTGGACTAAGGATGGCAACCCTTTTTATTTCACTGAC
CATCGGATAATTCCATCGAACAATTCAGGAACATTCAGGATCCCAAACGAGGGGCACATA
TCTCACTTTCAAGGGAAATACCGCTGCTTTGCTTCAAATAAACTGGGAATCGCTATGTCA
GAAGAAATAGAATTTATAGTTCCAAGTGTTCCAAAATTCCCAAAAGAAAAAATTGACCCT
CTTGAAGTGGAGGAGGGAGATCCAATTGTCCTCCCATGCAATCCTCCCAAAGGCCTCCCA
CCTTTACACATTTATTGGATGAATATTGAATTAGAACACATCGAACAAGATGAAAGAGTA
TACATGAGCCAAAAGGGAGATCTATACTTCGCAAACGTGGAAGAAAAGGACAGTCGCAAT
GACTACTGTTGCTTTGCTGCATTTCCAAGATTAAGGACTATTGTACAGAAAATGCCAATG
AAACTAACAGTTAACAGTTCAAATTCCATCAAGCAAAGAAAACCCAAACTGCTGTTGCCT
CCCACTGAGAGTGGCAGTGAGTCTTCAATTACCATCCTCAAAGGGGAAATCTTGCTGCTT
GAGTGTTTTGCTGAAGGCTTGCCAACTCCACAGGTTGATTGGAACAAAATTGGTGGTGAC
TTACCAAAGGGGAGAGAAGCAAAAGAAAATTATGGCAAGACTTTGAAGATAGAGAATGTC
TCCTACCAGGACAAAGGAAATTATCGCTGCACAGCCAGCAATTTCTTGGGAACAGCCACT
CACGATTTTCACGTTATAGTAGAAGAGCCTCCTCGCTGGACAAAGAAGCCTCAGAGTGCT
GTGTATAGCACCGGAAGCAATGGCATCTTGTTATGTGAGGCTGAAGGAGAACCTCAACCC
ACAATCAAGTGGAGAGTCAATGGCTCCCCAGTTGACAATCATCCATTTGCTGGTGATGTT
GTCTTCCCCAGGGAAATCAGTTTTACCAACCTTCAACCAAATCATACTGCTGTGTACCAG
TGTGAAGCCTCAAATGTCCATGGAACTATCCTTGCCAATGCCAATATTGATGTTGTGGAT
GTCCGTCCATTGATACAAACCAAAGATGGAGAAAATTACGCTACAGTGGTTGGGTACAGT
GCTTTCTTACATTGCGAGTTCTTTGCTTCACCTGAGGCAGTCGTGTCCTGGCAGAAGGTG
GAAGAAGTGAAACCCCTGGAGGGCAGGCGGTATCATATCTATGAAAATGGCACATTGCAG
ATCAACAGAACCACCGAAGAAGATGCTGGGTCTTACTCATGTTGGGTAGAAAATGCTATA
GGAAAAACTGCAGTCACAGCCAATTTGGATATTAGAAATGCTACAAAACTTAGAGTTTCT
CCTAAGAATCCTCGTATCCCCAAATTGCATATGCTTGAATTACATTGTGAAAGCAAATGT
GACTCACATTTGAAACACAGTTTGAAGTTGTCCTGGAGTAAAGATGGAGAAGCCTTTGAA
ATTAATGGCACAGAAGATGGCAGGATAATTATTGATGGAGCTAATTTGACCATATCTAAT
GTAACTTTAGAGGACCAAGGTATTTACTGCTGTTCAGCTCATACTGCTCTAGACAGTGCT
GCCGATATAACTCAAGTAACTGTTCTTGATGTTCCGGATCCACCAGAAAACCTTCACTTG
TCTGAAAGACAGAACAGGAGTGTTCGGCTGACCTGGGAAGCTGGAGCTGACCACAACAGC
AATATTAGCGAGTATATTGTTGAATTTGAAGGAAACAAAGAAGAGCCTGGAAGGTGGGAG
GAACTGACCAGAGTCCAAGGAAAGAAAACCACAGTTATCTTACCTTTGGCTCCATTTGTG
AGATACCAGTTCAGGGTCATAGCCGTGAACGAAGTAGGGAGAAGTCAGCCTAGCCAGCCG
TCAGACCATCATGAAACACCACCAGCAGCTCCAGATAGGAATCCACAAAACATAAGGGTT
CAAGCCTCTCAACCCAAGGAAATGATTATAAAGTGGGAGCCTTTGAAATCCATGGAGCAG
AATGGACCAGGCCTAGAGTACAGAGTGACCTGGAAGCCACAGGGAGCCCCAGTGGAGTGG
GAAGAAGAAACAGTCACAAACCACACATTGCGGGTGATGACGCCTGCTGTCTATGCCCCT
TATGATGTCAAGGTCCAGGCTATCAATCAACTAGGATCTGGGCCTGACCCTCAGTCAGTG
ACTCTCTATTCTGGAGAAGACTATCCTGATACAGCTCCAGTGATCCATGGGGTGGACGTT
ATAAACAGTACATTAGTTAAAGTTACCTGGTCAACAGTTCCAAAGGACAGAGTACATGGA
CGTCTGAAAGGCTATCAGATAAATTGGTGGAAAACAAAAAGTCTGTTGGATGGAAGAACA
CATCCCAAAGAAGTGAACATTCTAAGATTTTCAGGACAAAGAAACTCTGGAATGGTTCCT
TCCTTAGATGCCTTTAGTGAATTTCATTTAACAGTCTTAGCCTATAACTCTAAAGGAGCT
GGTCCTGAAAGTGAGCCTTATATATTTCAAACACCAGAAGGAGTACCTGAACAGCCAACT
TTTCTAAAGGTCATCAAAGTTGATAAAGACACTGCCACTTTATCTTGGGGACTACCTAAG
AAATTAAATGGAAACTTAACTGGCTATCTTTTGCAATATCAGATAATAAATGACACCTAC
GAGATTGGAGAATTAAATGATATTAACATTACAACTCCATCAAAGCCCAGCTGGCACCTC
TCAAACCTGAATGCAACTACCAAGTACAAATTCTACTTGAGGGCTTGCACTTCACAGGGC
TGTGGAAAACCGATCACGGAGGAAAGCTCCACCTTAGGAGAAGGGAGTAAAGGTATCGGG
AAGATATCAGGAGTAAATCTTACTCAAAAGACTCACCCAGTAGAGGTATTTGAGCCGGGA
GCTGAACATATAGTTCGCCTAATGACTAAGAATTGGGGCGATAACGATAGCATTTTTCAA
GATGTAATTGAGACAAGAGGGAGAGAATATGCTGGTTTATATGATGACATCTCCACTCAA
GGCTGGTTTATTGGACTGATGTGTGCGATTGCTCTTCTCACACTACTATTATTAACTGTT
TGCTTTGTGAAGAGGAATAGAGGTGGAAAGTACTCAGTTAAAGAAAAGGAAGATTTGCAT
CCAGACCCAGAAATTCAGTCAGTAAAAGATGAAACCTTTGGTGAATACAGTGACAGTGAT
GAAAAGCCTCTCAAAGGAAGCCTTCGGTCCCTTAATAGGGATATGCAGCCTACTGAAAGT
GCTGACAGCTTAGTCGAATACGGAGAGGGAGACCATGGTCTCTTCAGTGAAGATGGATCA
TTTATTGGTGCCTACGCTGGATCTAAGGAGAAGGGATCTGTTGAAAGCAATGGAAGTTCT
ACAGCAACTTTTCCCCTTCGGGCA
Protein sequence predicted based on nucleotide sequence
MEPLLLGRGLIVYLMFLLLKFSKAIEIPSSVQQVPTIIKQSKVQVAFPFDEYFQIECEAK GNPEPTFSWTKDGNPFYFTDHRIIPSNNSGTFRIPNEGHISHFQGKYRCFASNKLGIAMS EEIEFIVPSVPKFPKEKIDPLEVEEGDPIVLPCNPPKGLPPLHIYWMNIELEHIEQDERV YMSQKGDLYFANVEEKDSRNDYCCFAAFPRLRTIVQKMPMKLTVNSSNSIKQRKPKLLLP PTESGSESSITILKGEILLLECFAEGLPTPQVDWNKIGGDLPKGREAKENYGKTLKIENV SYQDKGNYRCTASNFLGTATHDFHVIVEEPPRWTKKPQSAVYSTGSNGILLCEAEGEPQP TIKWRVNGSPVDNHPFAGDVVFPREISFTNLQPNHTAVYQCEASNVHGTILANANIDVVD VRPLIQTKDGENYATVVGYSAFLHCEFFASPEAVVSWQKVEEVKPLEGRRYHIYENGTLQ INRTTEEDAGSYSCWVENAIGKTAVTANLDIRNATKLRVSPKNPRIPKLHMLELHCESKC DSHLKHSLKLSWSKDGEAFEINGTEDGRIIIDGANLTISNVTLEDQGIYCCSAHTALDSA ADITQVTVLDVPDPPENLHLSERQNRSVRLTWEAGADHNSNISEYIVEFEGNKEEPGRWE ELTRVQGKKTTVILPLAPFVRYQFRVIAVNEVGRSQPSQPSDHHETPPAAPDRNPQNIRV QASQPKEMIIKWEPLKSMEQNGPGLEYRVTWKPQGAPVEWEEETVTNHTLRVMTPAVYAP YDVKVQAINQLGSGPDPQSVTLYSGEDYPDTAPVIHGVDVINSTLVKVTWSTVPKDRVHG RLKGYQINWWKTKSLLDGRTHPKEVNILRFSGQRNSGMVPSLDAFSEFHLTVLAYNSKGA GPESEPYIFQTPEGVPEQPTFLKVIKVDKDTATLSWGLPKKLNGNLTGYLLQYQIINDTY EIGELNDINITTPSKPSWHLSNLNATTKYKFYLRACTSQGCGKPITEESSTLGEGSKGIG KISGVNLTQKTHPVEVFEPGAEHIVRLMTKNWGDNDSIFQDVIETRGREYAGLYDDISTQ GWFIGLMCAIALLTLLLLTVCFVKRNRGGKYSVKEKEDLHPDPEIQSVKDETFGEYSDSD EKPLKGSLRSLNRDMQPTESADSLVEYGEGDHGLFSEDGSFIGAYAGSKEKGSVESNGSS TATFPLRA
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
