ITSN2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ITSN2
Gene Name:  intersectin 2
Original Clone ID:
ccsbBroadEn_10457
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC146779.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516442
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 5007nts
Open reading frame: 1 to 5007

ATGGCTCAGTTTCCCACAGCTATGAATGGAGGGCCAAACATGTGGGCTATTACCTCTGAA
GAACGTACTAAGCATGACAGGCAGTTTGATAACCTCAAACCTTCAGGAGGTTACATAACA
GGTGATCAAGCACGTAATTTTTTCCTACAATCAGGTCTGCCGGCCCCTGTTTTAGCTGAA
ATATGGGCTTTATCAGACCTAAACAAGGATGGGAAGATGGATCAGCAAGAGTTCTCCATA
GCTATGAAACTCATCAAACTGAAGCTTCAAGGCCAACAGTTGCCTGTGGTTCTCCCTCCT
ATTATGAAGCAACCCCCTATGTTTTCTCCATTAATTTCTGCTCGTTTTGGAATGGGAAGC
ATGCCCAATCTGTCCATTCCTCAGCCATTGCCTCCAGCTGCACCTATAACATCATTGTCT
TCTGCGACTTCAGGGACCAACCTTCCTCCCTTAATGATGCCCACTCCCCTAGTGCCTTCT
GTTAGCACATCATCATTACCAAATGGAACCGCCAGTCTCATTCAGCCTTTACCCATTCCT
TATTCTTCTTCAACATTGCCTCATGGGTCATCTTATAGTCTGATGATGGGAGGATTTGGA
GGTGCTAGTATACAGAAAGCGCAGTCTCTGATTGATTTAGGATCTAGTAGCTCAACTTCC
TCGACTGCTTCACTCTCAGGGAACTCACCCAAGACTGGGACCTCAGAGTGGGCAGTTCCT
CAGCCTACAAGATTAAAATATCGGCAAAAATTTAATACTCTTGACAAAAGTATGAGTGGA
TATCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAATCTTTCTCAAACTCAG
CTGGCTACTATTTGGACTCTGGCTGACGTTGATGGTGATGGACAGCTAAAAGCAGAAGAG
TTTATTCTTGCAATGCACCTTACTGACATGGCCAAAGCTGGACAGCCATTACCACTGACT
TTACCTCCTGAGCTTGTTCCTCCATCTTTCAGAGGAGGAAAGCAAATTGATTCCATTAAT
GGAACTCTGCCTTCATATCAGAAAATGCAAGAAGAGGAGCCTCAGAAGAAATTACCAGTT
ACTTTTGAGGACAAACGGAAAGCCAACTATGAGCGAGGGAACATGGAGCTGGAAAAGCGA
CGCCAAGCCTTGATGGAGCAGCAACAAAGGGAGGCAGAACGTAAAGCCCAGAAAGAAAAG
GAAGAGTGGGAACGAAAACAGAGAGAATTACAAGAACAAGAATGGAAGAAACAACTTGAA
TTAGAAAAACGCTTAGAGAAGCAACGGGAATTGGAGAGACAACGAGAGGAAGAAAGGAGA
AAAGACATAGAAAGACGAGAGGCAGCAAAACAGGAACTTGAACGACAACGTCGCTTAGAA
TGGGAGAGAATTCGGCGACAGGAGCTTCTCAATCAAAAGAATAGAGAACAAGAAGAAATT
GTCAGGTTAAACTCTAAAAAGAAGAATCTTCATCTTGAGTTGGAAGCACTGAATGGCAAA
CATCAGCAGATCTCAGGCAGACTTCAGGATGTCCGACTCAAAAAGCAAACTCAAAAGACT
GAGCTGGAAGTTCTGGATAAGCAGTGTGACTTGGAAATTATGGAAATCAAGCAACTTCAA
CAGGAACTTCAGGAATATCAGAATAAGCTTATCTATCTGGTACCTGAGAAGCAATTATTA
AATGAAAGAATTAAAAACATGCAGTTCAGTAACACACCTGATTCAGGGGTCAGTTTACTT
CATAAAAAATCATTAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAGTTAGATGCT
CTTGAAAAAGAAACTGCATCTAAGCTGTCAGAAATGGATTCTTTTAACAATCAACTAAAG
GAACTGAGAGAAACCTACAACACACAGCAGTTAGCCCTTGAACAGCTTTATAAGATCAAA
CGTGACAAGTTGAAGGAAATTGAAAGGAAAAGATTAGAACTAATGCAGAAAAAGAAACTA
GAAGATGAGGCTGCAAGGAAAGCAAAGCAAGGAAAAGAAAACTTATGGAAAGAAAATCTT
AGAAAGGAGGAAGAAGAAAAACAAAAGCGACTCCAGGAAGAAAAAACACAAGAAAAAATT
CAAGAAGAGGAACGGAAAGCTGAGGAGAAACAACGTAAGGATAAGGATACTTTGAAAGCT
GAGGAGAAAAAACGTGAGACAGCTAGTGTTTTGGTGAATTATAGAGCATTATACCCCTTT
GAAGCAAGGAACCATGATGAGATGAGTTTTAATTCTGGAGATATAATTCAGGTTGATGAA
AAAACCGTAGGAGAACCTGGTTGGCTTTATGGTAGTTTTCAAGGAAATTTTGGCTGGTTT
CCATGCAATTATGTAGAAAAAATGCCATCAAGTGAAAATGAAAAAGCTGTATCTCCAAAG
AAGGCCTTACTTCCTCCTACAGTTTCTTTATCTGCTACCTCAACTTCCTCTGAACCACTT
TCTTCAAATCAACCAGCATCAGTGACTGATTATCAAAATGTATCTTTTTCAAACCTAACT
GTAAATACATCATGGCAGAAAAAATCAGCCTTCACTCGAACTGTGTCCCCTGGATCTGTA
TCACCTATTCATGGACAGGGACAAGTGGTAGAAAACTTAAAAGCACAGGCCCTTTGTTCC
TGGACTGCAAAGAAAGATAACCACTTGAACTTCTCAAAACATGACATTATTACTGTCTTG
GAGCAGCAAGAAAATTGGTGGTTTGGGGAGGTGCATGGAGGAAGAGGATGGTTTCCCAAA
TCTTATGTCAAGATCATTCCTGGGAGTGAAGTAAAACGGGAAGAACCAGAAGCTTTGTAT
GCAGCTGTAAATAAGAAACCTACCTCGGCAGCCTATTCAGTTGGAGAAGAATATATTGCA
CTTTATCCATATTCAAGTGTGGAACCTGGAGATTTGACTTTCACAGAAGGTGAAGAAATA
TTGGTGACCCAGAAAGATGGAGAGTGGTGGACAGGAAGTATTGGAGATAGAAGTGGAATT
TTTCCATCAAACTATGTCAAACCAAAGGATCAAGAGAGTTTTGGGAGTGCTAGCAAGTCT
GGAGCATCAAATAAAAAACCTGAGATTGCTCAGGTAACTTCAGCATATGTTGCTTCTGGT
TCTGAACAACTTAGCCTTGCACCAGGACAGTTAATATTAATTCTAAAGAAAAATACAAGT
GGGTGGTGGCAAGGAGAGTTACAGGCCAGAGGAAAAAAGCGACAGAAAGGATGGTTTCCT
GCCAGTCATGTTAAACTTTTGGGTCCAAGTAGTGAAAGAGCCACACCTGCCTTTCATCCT
GTATGTCAGGTGATTGCTATGTATGACTATGCAGCAAATAATGAAGATGAGCTCAGTTTC
TCCAAGGGACAACTCATTAATGTTATGAACAAAGATGATCCTGATTGGTGGCAAGGAGAG
ATCAACGGGGTGACTGGTCTCTTTCCTTCAAACTACGTTAAGATGACGACAGACTCAGAT
CCAAGTCAACAGTGGTGTGCTGATCTGCAAACCCTGGACACAATGCAGCCAATTGAGAGG
AAAAGACAGGGCTATATTCATGAGCTGATTCAGACCGAAGAGCGGTACATGGCTGACCTT
CAGCTCGTCGTCGAGGTTTTTCAGAAACGCATGGCAGAGTCAGGCTTTCTCACTGAAGGG
GAGATGGCCCTGATTTTTGTTAACTGGAAGGAGCTCATCATGTCCAACACAAAGCTGCTG
AAGGCTTTGCGGGTGCGGAAGAAGACCGGGGGCGAGAAGATGCCGGTGCAGATGATTGGG
GACATCCTGGCCGCTGAGCTGTCCCACATGCAGGCTTACATCAGGTTCTGCAGCTGCCAG
CTTAATGGAGCAGCTCTGTTACAGCAGAAGACAGATGAAGACACAGATTTCAAAGAATTT
TTAAAGAAGCTGGCATCTGACCCGCGGTGTAAAGGAATGCCCCTCTCCAGCTTCCTGCTG
AAACCCATGCAGAGGATCACCCGCTACCCACTGCTCATCAGAAGTATTCTGGAGAACACC
CCGGAGAGCCATGCAGACCATTCCTCCCTAAAGCTGGCCCTCGAGCGGGCAGAGGAGCTG
TGCTCTCAAGTGAATGAGGGAGTTCGGGAGAAGGAAAACTCGGACCGACTGGAGTGGATC
CAGGCGCACGTGCAGTGTGAAGGCCTCGCGGAGCAACTTATTTTCAACTCTCTCACCAAC
TGCCTGGGGCCCCGGAAGCTCTTACACAGTGGGAAATTATACAAGACCAAGAGCAACAAG
GAACTGCACGGATTCCTCTTCAATGACTTCCTGCTTCTTACCTACATGGTCAAGCAGTTT
GCTGTTTCCTCTGGCTCTGAGAAACTTTTCAGCTCGAAGTCCAATGCTCAATTCAAAATG
TATAAAACGCCCATTTTCCTGAATGAAGTCTTGGTGAAACTGCCCACAGACCCTTCCAGC
GATGAGCCTGTCTTCCACATTTCCCACATTGATCGGGTCTACACCCTCCGAACAGACAAC
ATTAATGAGAGGACCGCCTGGGTGCAGAAGATCAAGGCGGCGTCTGAGCAGTACATCGAC
ACCGAGAAGAAGCAGCGTGAGAAAGCTTACCAAGCCCGCTCCCAAAAGACTTCAGGCATT
GGGCGCCTGATGGTGCATGTCATTGAAGCTACAGAATTAAAAGCCTGCAAACCAAATGGA
AAGAGCAACCCATACTGTGAAATCAGCATGGGCTCCCAGAGCTACACCACCAGGACCATC
CAGGACACACTCAATCCCAAGTGGAATTTTAACTGCCAGTTCTTTATTAAGGATCTCTAC
CAAGACGTGCTGTGTCTCACCCTGTTTGACAGAGACCAGTTTTCACCAGATGATTTCCTG
GGTCGTACTGAAATTCCAGTGGCAAAAATTCGAACAGAACAGGAAAGCAAAGGCCCTATG
ACCCGCCGACTGCTGCTGCATGAGGTCCCCACCGGGGAGGTCTGGGTCCGTTTTGACCTG
CAGCTTTTTGAGCAAAAAACTCTCCTG

Protein sequence predicted based on nucleotide sequence

MAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAE
IWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGS
MPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIP
YSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVP
QPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEE
FILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPV
TFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLE
LEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEI
VRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQ
QELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDA
LEKETASKLSEMDSFNNQLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKL
EDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKA
EEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWF
PCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLT
VNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVL
EQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIA
LYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKS
GASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFP
ASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGE
INGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADL
QLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIG
DILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLL
KPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWI
QAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQF
AVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDN
INERTAWVQKIKAASEQYIDTEKKQREKAYQARSQKTSGIGRLMVHVIEATELKACKPNG
KSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFL
GRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable