Gene:
Gene Symbol:
NISCH
Gene Name:
nischarin
Original Clone ID:
ccsbBroadEn_07768
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC056900.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516449
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGACCGCGCGCACCTTCGGGCCCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGC
GTCGTGGGCTCGGAGCTTGTGGACACTTATACGGTTTACATCATCCAGGTCACTGATGGC
AGCCATGAGTGGACAGTAAAGCACCGCTACAGCGACTTCCATGACCTGCATGAAAAGCTC
GTTGCAGAGAGAAAGATTGATAAAAACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAAC
TCAAGAAGCTTGGTGGAGAAGAGGGAGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTG
GCTGCCTTCCCTGGCGTGACCCCCAGAGTACTGGCCCACTTCTTGCATTTTCACTTCTAT
GAGATAAATGGCATCACCGCGGCACTGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTC
CTGGGGGCCGGCGAGGTCTTTGCCATTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAG
CTGCAGCAGGGAAAGCCCACGTGCGCCAGTGGGGATGCCAAGACCGACCTCGGGCACATC
CTGGACTTCACCTGTCGCCTTAAGTACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGG
ACCAGCAACATTCAGGAGCAGCTCCTGCCGTTCGACCTATCAATATTCAAGTCCCTGCAT
CAGGTGGAGATAAGTCACTGTGATGCTAAGCACATCAGAGGGCTGGTCGCATCGAAGCCC
ACCTTAGCCACGCTGAGTGTCCGCTTCTCAGCAACCTCGATGAAGGAAGTCCTTGTTCCT
GAAGCCTCAGAATTTGATGAGTGGGAGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACT
GCCGTCATCCCCACTTGGCAGGCATTGACCACGCTTGACCTGAGCCACAACAGCATCTCC
GAGATCGACGAGTCTGTGAAACTGATCCCAAAGATTGAGTTCCTGGACCTGAGTCACAAT
GGATTGCTGGTTGTGGACAATCTGCAGCACCTGTATAACCTTGTGCATCTGGACCTGTCC
TACAACAAGCTCTCCTCCTTGGAAGGGCTTCACACCAAGCTGGGGAACATCAAGACCTTA
AACCTGGCAGGCAACCTCCTAGAGAGTCTGAGTGGCCTGCACAAGCTCTACTCACTGGTC
AACCTGGATCTCCGGGACAACAGGATCGAACAGATGGAGGAGGTCCGGAGCATAGGCAGC
CTCCCGTGTCTGGAGCACGTGTCTCTGCTGAACAACCCTCTGAGCATCATCCCCGACTAC
CGGACCAAGGTGCTGGCTCAGTTCGGAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACA
GTGACCACAGAGAAGGAGCTGGACACTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAG
GAGGTCAAGTCCAAACTGAGCAACCCAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCA
GCTGCCCCCTGCATCAGACCCAGCAGCTCCCCTCCCACTGTGGCTCCCGCATCTGCCTCC
CTGCCCCAGCCCATCCTCTCTAACCAAGGAATCATGTTCGTTCAGGAGGAGGCCCTGGCC
AGCAGCCTCTCGTCCACTGACAGTCTGACTCCCGAGCACCAGCCCATTGCCCAGGGATGT
TCTGATTCCTTGGAGTCCATCCCTGCGGGACAGGCAGCTTCCGATGATTTAAGGGACGTG
CCAGGAGCTGTTGGTGGTGCAAGCCCAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCG
GGGTCTGGCCAGATCATCTTCCTGCCCTTCACCTGCATTGGCTACACGGCCACCAATCAG
GACTTCATCCAGCGCCTGAGCACACTGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCC
TGGATCGAGGCTGCCAACCAGCGGGAGGAGGGCCAGGGTGAACAGGGCGAGGAGGAGGAT
GAGGAGGAGGAAGAAGAGGAGGACGTGGCTGAGAACCGCTACTTTGAAATGGGGCCCCCA
GACGTGGAGGAGGAGGAGGGAGGAGGCCAGGGGGAGGAAGAGGAGGAGGAAGAGGAGGAT
GAAGAGGCCGAGGAGGAGCGCCTGGCTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTC
CTGCTGGAGCACATCCGCATCCTCAAGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGC
AGTATCCGCCAGTTCGCCGCCTGCCTTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAG
ATCCCGCACCAGGAGTCTCGGGGCAGCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTC
TTTTGCTTCCCGCATGGCGACCTCACCGAGTTTGGCTTCCTCATGCCGGAGCTGTGTCTG
GTGCTCAAGGTACGGCACAGTGAGAACACGCTCTTCATTATCTCGGACGCCGCCAACCTG
CACGAGTTCCACGCGGACCTGCGCTCATGCTTTGCACCCCAGCACATGGCCATGCTGTGT
AGCCCCATCCTCTACGGCAGCCACACCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACC
TTCTACAAGGTGGCTGGCGGCTGCCAGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTG
GTCTACAGTGACAAGCGCATGGTGCAGACGGCCGCCGGGGACTACTCAGGCAACATCGAG
TGGGCCAGCTGCACACTCTGTTCAGCCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCC
GTCAAGTCCGCCGCCATCCCCTACTGGCTGTTGCTCACGCCCCAGCACCTCAACGTCATC
AAGGCCGACTTCAACCCCATCCCCAACCGTGGCACCCACAACTGTCGCAACCGCAACAGC
TTCAAGCTCAGCCGTGTGCCGCTCTCCACCGTGCTGCTGGACCCCACACGCAGCTGTACC
CAGCCTCGGGGCGCCTTTGCTGATGGCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTT
GTCACTGCCATCTTCGTGCTGCCCCACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAG
CTGCGGGCCTCGCTGCAGGACCTGAAGACTGTGGTCATCGCCAAGACCCCCGGGACGGGA
GGCAGCCCCCAGGGCTCCTTTGCGGATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGAC
CAGCGTCCCCAGGAGGTCCCAGCAGAGGCTCTGGCCCCGGCCCCAGTGGAAGTCCCAGCT
CCAGCCCCTGCAGCAGCCTCAGCCTCAGGCCCAGCGAAGACTCCGGCCCCAGCAGAGGCC
TCAACTTCAGCTTTGGTCCCAGAGGAGACGCCAGTGGAAGCTCCAGCCCCACCCCCAGCC
GAGGCCCCTGCCCAGTACCCGAGTGAGCACCTCATCCAGGCCACCTCGGAGGAGAATCAG
ATCCCCTCGCACTTGCCTGCCTGCCCGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGC
GCCATCATCGAGCTCTTCCACAGCAGCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCAC
CTCATGTGGTCCTCGGTGGTGTTCTACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTG
CTGCTCTCCACCAAGGCTGTGTACTTTGTGCTCCACGACGGCCTCCGCCGCTACTTCTCA
GAGCCACTGCAGGATTTCTGGCATCAGAAAAACACCGACTACAACAACAGCCCTTTCCAC
ATCTCCCAGTGCTTCGTGCTAAAGCTTAGTGACCTGCAGTCAGTCAATGTGGGGCTTTTC
GACCAGCATTTCCGGCTGACGGGTTCCACCCCGATGCAGGTGGTCACGTGCTTGACGCGG
GACAGCTACCTGACGCACTGCTTCCTCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAA
CGCACGCCCTCGCCGGAGCCTGTTGACAAGGACTTCTACTCCGAGTTTGGGAACAAGACC
ACAGGGAAGATGGAGAACTACGAGCTGATCCACTCTAGTCGCGTCAAGTTTACCTACCCC
AGTGAGGAGGAGATTGGGGACCTGACGTTCACTGTGGCCCAAAAGATGGCTGAGCCAGAG
AAGGCCCCAGCCCTCAGCATCCTGCTGTACGTGCAGGCCTTCCAGGTGGGCATGCCACCC
CCTGGGTGCTGCAGGGGCCCCCTGCGCCCCAAGACACTCCTGCTCACCAGCTCCGAGATC
TTCCTCCTGGATGAGGACTGTGTCCACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCG
CAGAGAGACAGGTACCGGCTGGACGATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTC
ATGGGCTACCAGACCTACCCGCAGGCCCTCACCCTCGTCTTCGATGACGTGCAAGGTCAT
GACCTCATGGGCAGTGTCACCCTGGACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGA
GCCAGCCAGGGCCGTGAAGTCCAGTGGCAGGTGTTTGTCCCCAGTGCTGAGAGCAGAGAG
AAGCTCATCTCGCTGTTGGCTCGCCAGTGGGAGGCCCTGTGTGGCCGTGAGCTGCCTGTC
GAGCTCACCGGC
Protein sequence predicted based on nucleotide sequence
MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKL VAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFY EINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHI LDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKP TLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSIS EIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTL NLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDY RTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLS AAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGC SDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQ DFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPP DVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQG SIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCL VLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLT FYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEA VKSAAIPYWLLLTPQHLNVIKADFNPIPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCT QPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTG GSPQGSFADGQPAERRASNDQRPQEVPAEALAPAPVEVPAPAPAAASASGPAKTPAPAEA STSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGS AIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFS EPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTR DSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYP SEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEI FLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGH DLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPV ELTG
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
