Gene:
Gene Symbol:
USP4
Gene Name:
ubiquitin specific peptidase 4 (proto-oncogene)
Original Clone ID:
ccsbBroadEn_07120
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC125130.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516469
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGACTCAGAAGTCCGAGCTT
GGACCCTTAATGAGGACCACACTCCAACGCGGGGCGCAGTGGTATCTTATTGACAGCCGG
TGGTTCAAGCAGTGGAAGAAGTATGTGGGCTTTGACAGCTGGGACATGTACAATGTGGGT
GAACATAACCTATTTCCTGGCCCAATAGACAACTCTGGGCTATTTTCAGATCCTGAGAGT
CAGACCTTGAAAGAACACTTAATTGATGAATTGGACTATGTATTGGTCCCTACCGAGGCG
TGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCAACCCATCGTCAGAAAA
GTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTCGAGGTGTATTTGCTGGAACTG
AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGAC
ACCATTGCAACCATCGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAA
ACACGGCTCTGGAACAAATACATGAGCAACACCTACGAGCAGTTGAGCAAACTAGACAAC
ACTGTCCAGGATGCTGGGCTATACCAGGGTCAGGTGCTAGTAATTGAGCCTCAAAATGAA
GATGGCACATGGCCCAGGCAGACCTTGCAGTCAAAATCAAGCACTGCGCCTAGCAGAAAT
TTTACTACCTCTCCAAAATCATCAGCAAGTCCCTATTCCTCAGTGTCTGCCTCTCTCATT
GCAAATGGTGATAGCACTAGCACCTGTGGGATGCACAGTTCCGGTGTCAGCAGGGGTGGA
TCTGGCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAACCTGGG
CTCTGTGGACTTGGAAACCTGGGAAACACCTGCTTCATGAACTCCGCTTTGCAGTGTTTG
AGCAACACTGCACCACTGACTGACTACTTTCTCAAAGATGAGTATGAAGCCGAAATCAAC
AGAGACAACCCTCTGGGGATGAAAGGGGAAATTGCAGAAGCCTATGCTGAACTCATTAAG
CAGATGTGGTCTGGAAGGGATGCCCATGTGGCACCTCGCATGTTCAAAACTCAAGTAGGA
CGTTTTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCTGCTGGCCTTT
CTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAAGCCCTACTTGGAGCTG
AAGGATGCCAATGGGCGGCCAGATGCGGTGGTGGCAAAGGAAGCCTGGGAGAATCACAGG
TTGAGGAATGATTCTGTGATTGTGGATACTTTCCATGGCCTCTTCAAATCTACTTTGGTT
TGCCCAGAATGTGCTAAGGTTTCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCA
CTGCCCTTGAAGAAAGATCGAGTTATGGAGGTTTTCCTGGTTCCTGCTGACCCTCACTGC
AGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGGCTGTGTCCGACCTGTGCGAG
GCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCGCAGATGTGTATAAT
CACCGATTCCACAAAATTTTCCAAATGGATGAAGGTTTAAACCACATCATGCCTCGGGAT
GACATTTTCGTGTACGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTT
CCAGTCTACTTCAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCAGCGCTA
TATGGGCAGCCACTATTGCTTTCTGTCCCCAAGCACAAGTTAACCCTTGAGTCTTTGTAC
CAGGCTGTTTGTGATCGTATCAGCCGCTATGTGAAACAGCCTTTACCTGATGAGTTTGGC
AGCTCACCCTTGGAGCCAGGGGCCTGCAATGGCTCCAGGAACAGCTGTGAAGGAGAAGAT
GAGGAAGAAATGGAGCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAACAGAAGGCAGT
GGGGAAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAAGGCCAG
CCCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTATGGAACAGCTGACATA
AATTCACTTGCAGCTGATGGAAAACTACTTAAACTCAACTCTCGATCTACACTGGCCATG
GATTGGGACAGTGAAACTCGGAGACTTTACTATGATGAGCAAGAATCTGAGGCCTACGAG
AAGCATGTGAGCATGTTGCAGCCTCAGAAGAAGAAGAAGACCACAGTGGCCCTGAGAGAC
TGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGACCCCTGGTACTGTCCC
AACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATGGTCCTTGCCCAAGATC
CTGGTGGTCCACCTCAAACGTTTCTCCTACAACAGATACTGGAGGGATAAGCTCGACACA
GTCGTAGAATTCCCAATCAGAGGGCTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCA
AGGCCTTATGTGTACGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGC
CACTACACTGCATATGCGAAGAACAAACTGAATGGTAAATGGTATTACTTTGATGATAGC
AACGTGTCCCTGGCCTCTGAGGATCAGATAGTGACTAAAGCAGCTTATGTGCTATTTTAC
CAACGTCGAGATGATGAATTTTATAAGACACCTTCACTTAGCAGTTCTGGTTCCTCTGAT
GGAGGGACACGACCAAGCAGCTCTCAGCAGGGCTTTGGGGATGATGAGGCTTGCAGCATG
GACACCAAC
Protein sequence predicted based on nucleotide sequence
MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVG EHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRK VVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERE TRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRN FTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPG LCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIK QMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLEL KDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLP LPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYN HRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASAL YGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGED EEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADI NSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRD CIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDT VVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDS NVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSM DTN
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
