CYTSA (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SPECC1L
Gene Name:  sperm antigen with calponin homology and coiled-coil domains 1-like
Original Clone ID:
ccsbBroadEn_07875
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC136471.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516483
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3351nts
Open reading frame: 1 to 3351

ATGAAGAAAGCAAGCAGGAGTGTTGGCTCAGTGCCTAAAGTGTCTGCAATAAGTAAAACG
CAAACAGCAGAAAAAATTAAACCTGAAAACAGCTCTTCAGCATCTACGGGAGGCAAACTT
GTAAAACCTGGAACAGCAGCATCATTGTCAAAGACCAAGAGCAGTGATGACCTTTTAGCT
GGAATGGCCGGAGGGGTAACGGTGACTAATGGTGTTAAAGGAAAGAAAAGCACCTGCCCA
TCTGCAGCACCTTCAGCATCTGCCCCTGCCATGACCACCGTGGAGAACAAATCCAAGATT
AGCACAGGCACAGCTTCTTCAACCAAGCGGAGCACTTCTACAGGTAATAAAGAATCCAGT
TCTACTAGAGAAAGATTACGTGAACGTACCCGATTAAACCAGAGCAAAAAACTACCTTCT
GCAGGTCAGGGAGCTAATGACATGGCATTGGCCAAACGTTCCCGCAGTCGAACTGCTACA
GAATGTGACGTTCGTATGAGCAAGTCTAAGTCAGACAATCAGATCAGTGACAGAGCTGCT
TTGGAGGCCAAAGTGAAGGATCTTCTCACGCTGGCAAAAACCAAAGACGTAGAAATTTTA
CATTTGAGAAATGAACTGCGAGACATGCGTGCCCAGCTGGGCATTAATGAGGATCATTCT
GAGGGTGATGAAAAATCTGAGAAGGAAACTATTATGGCTCACCAGCCGACTGATGTGGAG
TCCACTTTATTGCAGTTGCAGGAACAGAATACTGCCATCCGTGAAGAACTCAACCAGCTG
AAAAATGAAAACAGAATGTTAAAGGACAGGTTGAATGCATTGGGCTTTTCCCTAGAGCAG
AGGTTAGACAATTCTGAAAAACTGTTTGGCTATCAGTCCCTGAGCCCAGAAATCACCCCT
GGTAACCAGAGCGATGGAGGAGGAACTCTGACTTCTTCAGTGGAAGGCTCTGCCCCTGGC
TCAGTGGAGGATCTCTTGAGTCAGGATGAAAATACACTAATGGACCATCAGCACAGTAAC
TCCATGGACAATTTAGACAGTGAGTGCAGTGAGGTCTACCAGCCCCTCACATCGAGCGAT
GATGCGCTGGATGCACCATCCTCCTCAGAGTCGGAAGGCATCCCCAGCATAGAGCGCTCC
CGGAAGGGGAGCAGCGGGAATGCCAGTGAAGTGTCCGTGGCTTGCCTGACTGAACGGATA
CACCAGATGGAAGAGAACCAACACAGTACAAGTGAGGAACTCCAGGCAACCCTGCAAGAG
CTAGCTGATTTACAGCAGATTACCCAGGAACTGAATAGTGAAAACGAAAGGCTTGGAGAA
GAGAAGGTTATTCTGATGGAGTCTTTATGTCAGCAGAGCGATAAGTTGGAACACTTTAGT
CGACAGATTGAATACTTCCGCTCTCTTCTAGATGAGCATCACATTTCTTATGTCATAGAT
GAAGATGTAAAAAGTGGGCGCTATATGGAATTAGAGCAACGTTACATGGACCTCGCTGAG
AATGCCCGTTTTGAACGGGAGCAGCTTCTTGGTGTCCAGCAGCATTTAAGCAATACTTTG
AAAATGGCAGAACAAGACAATAAGGAAGCTCAAGAAATGATAGGGGCACTCAAAGAACGC
AGTCACCATATGGAGCGAATTATTGAGTCTGAGCAGAAAGGAAAAGCAGCCTTGGCAGCC
ACGTTAGAGGAATACAAAGCCACAGTGGCCAGTGACCAGATAGAGATGAATCGCCTGAAG
GCTCAGCTGGAGAATGAAAAGCAGAAAGTGGCAGAGCTGTATTCTATCCATAACTCTGGA
GACAAATCTGATATTCAGGACCTCCTGGAGAGTGTCAGGCTGGACAAAGAAAAAGCAGAG
ACTTTGGCTAGTAGCTTGCAGGAAGATCTGGCTCATACCCGAAATGATGCCAATCGATTA
CAGGATGCCATTGCTAAGGTAGAGGATGAATACCGAGCCTTCCAAGAAGAAGCTAAGAAA
CAAATTGAAGATTTGAATATGACGTTAGAAAAATTAAGATCAGACCTGGATGAAAAAGAA
ACAGAAAGGAGTGACATGAAAGAAACCATCTTTGAACTTGAAGATGAAGTAGAACAACAT
CGTGCTGTGAAACTTCATGACAACCTCATTATTTCTGATCTAGAGAATACAGTTAAAAAA
CTCCAGGACCAAAAGCACGACATGGAAAGAGAAATAAAGACACTCCACAGAAGACTTCGG
GAAGAATCTGCGGAATGGCGGCAGTTTCAGGCTGATCTCCAGACTGCAGTAGTCATTGCA
AATGACATTAAATCTGAAGCCCAAGAGGAGATTGGTGATCTAAAGCGCCGGTTACATGAG
GCTCAAGAAAAAAATGAGAAACTCACAAAAGAATTGGAGGAAATAAAGTCACGCAAGCAA
GAGGAGGAGCGAGGCCGGGTATACAATTACATGAATGCCGTTGAGAGAGATTTGGCAGCC
TTAAGGCAGGGAATGGGACTGAGTAGAAGGTCCTCGACTTCCTCAGAGCCAACTCCTACA
GTAAAAACCCTCATCAAGTCCTTTGACAGTGCATCTCAAGTACCAAACCCTGCTGCAGCT
GCAATTCCTCGAACGCCCCTGAGCCCAAGTCCTATGAAAACCCCTCCTGCAGCAGCTGTG
TCCCCTATGCAGAGACATTCCATAAGTGGACCAATCTCAACATCCAAACCCCTGACAGCC
CTGTCAGATAAGAGACCAAACTATGGGGAAATCCCTGTTCAAGAGCATCTGTTAAGAACA
TCTTCAGCCAGCCGGCCTGCTTCCCTGCCAAGAGTGCCTGCGATGGAAAGTGCCAAGACC
CTCTCAGTGTCTCGACGAAGTAGTGAAGAAATGAAACGGGACATTTCTGCACAGGAGGGA
GCGTCGCCAGCCTCTCTGATGGCTATGGGAACCACGTCTCCACAGCTTTCCCTGTCCTCT
TCTCCAACGGCATCTGTGACTCCCACCACCCGAAGCCGAATAAGAGAAGAAAGGAAAGAC
CCTCTCTCAGCATTGGCCAGAGAATATGGAGGATCAAAGAGGAACGCCTTGCTGAAGTGG
TGTCAGAAGAAAACAGAAGGCTATCAGAATATTGACATTACAAACTTCAGCAGCAGCTGG
AATGATGGGCTGGCCTTCTGTGCCCTCCTGCATACATATCTCCCTGCCCACATTCCATAT
CAAGAACTGAACAGCCAGGATAAGAGAAGGAACTTCATGCTGGCTTTCCAGGCAGCTGAA
AGTGTCGGCATCAAATCCACACTGGACATTAATGAAATGGTACGGACTGAACGACCCGAC
TGGCAGAACGTGATGCTGTATGTGACGGCGATCTACAAGTACTTTGAGACC

Protein sequence predicted based on nucleotide sequence

MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLA
GMAGGVTVTNGVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESS
STRERLRERTRLNQSKKLPSAGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAA
LEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGINEDHSEGDEKSEKETIMAHQPTDVE
STLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQRLDNSEKLFGYQSLSPEITP
GNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECSEVYQPLTSSD
DALDAPSSSESEGIPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQE
LADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVID
EDVKSGRYMELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKER
SHHMERIIESEQKGKAALAATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSG
DKSDIQDLLESVRLDKEKAETLASSLQEDLAHTRNDANRLQDAIAKVEDEYRAFQEEAKK
QIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKK
LQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHE
AQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPT
VKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTA
LSDKRPNYGEIPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEMKRDISAQEG
ASPASLMAMGTTSPQLSLSSSPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKW
CQKKTEGYQNIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKRRNFMLAFQAAE
SVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable