Gene:
Gene Symbol:
PCDH9
Gene Name:
protocadherin 9
Original Clone ID:
ccsbBroadEn_06693
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC136626.1
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516491
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGACCTGAGGGATTTTTACCTGTTGGCTGCTCTGATTGCCTGTTTAAGGCTGGATTCC
GCAATAGCTCAAGAACTTATTTACACTATTAGAGAGGAATTGCCTGAAAATGTGCCCATA
GGAAACATACCAAAGGATCTGAACATTTCTCACATCAATGCTGCCACAGGGACCAGCGCC
AGCCTTGTCTACAGACTGGTTTCTAAAGCTGGGGATGCCCCTTTGGTGAAAGTATCCAGC
AGCACTGGGGAAATTTTCACAACCTCCAACAGAATAGACAGAGAAAAACTCTGTGCTGGC
GCCTCATATGCTGAGGAGAATGAGTGTTTCTTTGAACTTGAGGTGGTGATCCTCCCCAAT
GATTTCTTCAGGCTGATCAAAATAAAAATAATTGTCAAGGATACCAATGATAATGCCCCC
ATGTTTCCATCTCCTGTCATCAATATTTCCATTCCAGAAAACACTTTGATCAACAGCCGC
TTTCCAATTCCATCAGCAACAGATCCTGACACAGGCTTCAATGGTGTACAGCATTATGAA
TTGTTAAATGGGCAGAGTGTTTTTGGACTGGATATCGTGGAAACTCCAGAGGGAGAGAAG
TGGCCACAACTGATTGTTCAGCAAAACTTGGATAGAGAACAGAAAGATACCTATGTGATG
AAAATCAAAGTAGAGGATGGAGGCACTCCACAGAAATCCAGTACGGCCATACTGCAGGTC
ACAGTAAGTGATGTAAATGACAACAGGCCAGTGTTTAAAGAGGGTCAAGTGGAGGTGCAT
ATTCCAGAGAATGCTCCCGTAGGTACCTCTGTAATTCAGCTCCATGCCACTGATGCAGAT
ATAGGCAGTAATGCTGAAATCCGGTACATTTTTGGTGCCCAGGTCGCCCCTGCAACCAAA
AGACTCTTTGCTTTAAATAATACTACTGGGCTGATTACAGTTCAGAGGTCCTTAGATAGA
GAGGAGACAGCCATTCACAAAGTGACAGTGCTGGCTAGTGACGGCAGCTCCACTCCTGCT
CGAGCAACGGTTACCATCAATGTCACCGATGTAAATGATAACCCTCCTAATATAGACCTC
AGGTACATTATAAGTCCCATCAATGGCACCGTGTATTTATCTGAGAAAGATCCTGTCAAT
ACAAAGATTGCCCTAATTACAGTTTCAGATAAGGACACAGATGTGAATGGCAAAGTGATC
TGTTTTATTGAAAGAGAGGTCCCATTTCATTTGAAGGCGGTATATGACAACCAATATTTG
TTAGAGACCTCTTCTTTGTTGGACTATGAGGGCACCAAAGAATTCAGCTTTAAAATTGTT
GCCTCTGATTCTGGGAAGCCCAGTTTAAATCAGACTGCCCTGGTAAGGGTTAAGCTTGAG
GATGAAAATGACAACCCACCAATTTTCAACCAGCCTGTAATTGAGCTGTCAGTTTCTGAA
AACAACCGACGTGGGTTATACTTAACAACTATTAGTGCCACAGATGAAGACAGTGGGAAA
AATGCAGACATTGTTTATCAGCTTGGACCTAATGCCTCCTTCTTTGATCTGGACCGAAAA
ACAGGAGTTTTGACAGCCTCCAGAGTATTTGACAGAGAAGAACAAGAACGATTCATTTTT
ACAGTAACTGCCAGGGATAATGGGACCCCTCCCCTCCAAAGCCAAGCGGCTGTGATTGTT
ACTGTTCTGGATGAGAATGACAATAGCCCCAAGTTTACTCATAATCATTTTCAATTTTTT
GTGTCTGAGAATCTGCCAAAGTATAGTACTGTGGGGGTAATCACAGTGACAGATGCAGAT
GCTGGAGAGAATAAAGCTGTGACTCTTTCCATTCTAAATGACAATGATAATTTTGTGTTG
GATCCCTATTCTGGAGTCATAAAGTCAAATGTCTCATTTGATAGAGAGCAGCAGAGTTCC
TACACTTTTGATGTCAAAGCCACTGATGGAGGACAACCACCTCGTTCCTCTACTGCAAAA
GTAACTATCAACGTCATGGATGTCAATGACAACAGCCCAGTTGTCATTTCTCCACCGTCT
AATACTTCCTTTAAGTTGGTGCCCCTCTCAGCCATTCCTGGCTCCGTGGTAGCAGAAGTT
TTTGCAGTGGATGTTGACACTGGAATGAACGCTGAACTAAAGTATACTATAGTGAGTGGA
AACAATAAAGGCTTATTCCGGATTGATCCAGTAACAGGTAACATTACTCTGGAAGAAAAA
CCAGCACCTACTGATGTGGGATTGCATCGTTTGGTGGTCAACATAAGTGACCTGGGGTAC
CCTAAGTCTTTGCACACGCTTGTGCTTGTATTCCTTTATGTTAACGACACTGCTGGAAAT
GCCTCCTATATCTATGACTTGATCCGCAGGACTATGGAGACCCCGTTGGACAGGAACATA
GGGGATAGTAGCCAACCCTATCAAAATGAGGACTATCTAACCATCATGATTGCCATCATC
GCCGGTGCCATGGTGGTCATTGTTGTGATCTTCGTCACCGTTCTGGTGCGCTGTCGCCAT
GCATCAAGGTTCAAAGCAGCTCAGAGGAGCAAGCAAGGTGCCGAATGGATGTCCCCAAAC
CAGGAGAACAAGCAAAACAAGAAAAAGAAAAGAAAGAAAAGGAAGTCTCCCAAAAGCTCT
CTTTTGAACTTTGTTACTATCGAAGAGTCCAAACCCGATGATGCAGTTCATGAACCTATC
AATGGGACAATAAGCCTGCCGGCTGAACTGGAGGAGCAAAGTATAGGAAGATTTGACTGG
GGCCCGGCACCTCCAACAACATTCAAGCCTAACAGTCCTGACCTGGCCAAGCACTACAAA
TCTGCTTCTCCACAGCCTGCTTTTCATCTCAAACCAGACACTCCAGTTTCCGTGAAAAAG
CACCACGTGATTCAGGAACTCCCTTTGGACAACACCTTTGTTGGGGGTTGTGACACCCTT
TCTAAACGCTCTTCCACTAGTTCAGATCACTTCAGTGCCTCAGAGTGCAGTTCCCAAGGA
GGCTTCAAGACAAAGGGCCCCTTACACACCAGACAGTGTAACTCACACAGCAAAAGTGAC
AATATTCCTGTCACTCCTCAGAAATGTCCCAGCTCCACGGGTTTCCACATTCAGGAGAAT
GAAGAAAGCCATTACGAGTCGCAGCGCCGTGTTACGTTTCATCTCCCTGATGGCTCCCAG
GAAAGCTGCAGTGACAGTGGTCTAGGAGACCATGAGCCGGTGGGTAGTGGAACCCTGATC
TCACACCCTCTTCCTCTGGTTCAGCCACAGGACGAATTCTATGACCAGGCCTCTCCGGAC
AAGAGGACTGAAGCAGATGGCAACTCTGATCCCAACTCTGATGGGCCTTTGGGTCCCCGA
GGATTAGCTGAAGCTACAGAGATGTGCACTCAAGAGTGCTTGGTTTTGGGTCACTCTGAT
AATTGCTGGATGCCTCCTGGCTTGGGTCCATATCAACACCCCAAATCTCCTCTCTCAACC
TTTGCACCCCAGAAAGAATGGGTGAAGAAGGACAAGCTTGTGAATGGGCACACCCTGACC
AGAGCCTGGAAAGAAGACAGCAACAGGAACCAGTTCAATGACCGTAAGCAGTATGGCTCC
AATGAAGGCCATTTCAACAATGGCAGCCACATGACAGACATTCCTCTGGCAAATCTGAAG
TCTTATAAGCAAGCAGGAGGTGCTACTGAGAGTCCTAAGGAGCACCAACTC
Protein sequence predicted based on nucleotide sequence
MDLRDFYLLAALIACLRLDSAIAQELIYTIREELPENVPIGNIPKDLNISHINAATGTSA SLVYRLVSKAGDAPLVKVSSSTGEIFTTSNRIDREKLCAGASYAEENECFFELEVVILPN DFFRLIKIKIIVKDTNDNAPMFPSPVINISIPENTLINSRFPIPSATDPDTGFNGVQHYE LLNGQSVFGLDIVETPEGEKWPQLIVQQNLDREQKDTYVMKIKVEDGGTPQKSSTAILQV TVSDVNDNRPVFKEGQVEVHIPENAPVGTSVIQLHATDADIGSNAEIRYIFGAQVAPATK RLFALNNTTGLITVQRSLDREETAIHKVTVLASDGSSTPARATVTINVTDVNDNPPNIDL RYIISPINGTVYLSEKDPVNTKIALITVSDKDTDVNGKVICFIEREVPFHLKAVYDNQYL LETSSLLDYEGTKEFSFKIVASDSGKPSLNQTALVRVKLEDENDNPPIFNQPVIELSVSE NNRRGLYLTTISATDEDSGKNADIVYQLGPNASFFDLDRKTGVLTASRVFDREEQERFIF TVTARDNGTPPLQSQAAVIVTVLDENDNSPKFTHNHFQFFVSENLPKYSTVGVITVTDAD AGENKAVTLSILNDNDNFVLDPYSGVIKSNVSFDREQQSSYTFDVKATDGGQPPRSSTAK VTINVMDVNDNSPVVISPPSNTSFKLVPLSAIPGSVVAEVFAVDVDTGMNAELKYTIVSG NNKGLFRIDPVTGNITLEEKPAPTDVGLHRLVVNISDLGYPKSLHTLVLVFLYVNDTAGN ASYIYDLIRRTMETPLDRNIGDSSQPYQNEDYLTIMIAIIAGAMVVIVVIFVTVLVRCRH ASRFKAAQRSKQGAEWMSPNQENKQNKKKKRKKRKSPKSSLLNFVTIEESKPDDAVHEPI NGTISLPAELEEQSIGRFDWGPAPPTTFKPNSPDLAKHYKSASPQPAFHLKPDTPVSVKK HHVIQELPLDNTFVGGCDTLSKRSSTSSDHFSASECSSQGGFKTKGPLHTRQCNSHSKSD NIPVTPQKCPSSTGFHIQENEESHYESQRRVTFHLPDGSQESCSDSGLGDHEPVGSGTLI SHPLPLVQPQDEFYDQASPDKRTEADGNSDPNSDGPLGPRGLAEATEMCTQECLVLGHSD NCWMPPGLGPYQHPKSPLSTFAPQKEWVKKDKLVNGHTLTRAWKEDSNRNQFNDRKQYGS NEGHFNNGSHMTDIPLANLKSYKQAGGATESPKEHQL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
