Gene:
Gene Symbol:
BMS1
Gene Name:
BMS1 ribosome biogenesis factor
Original Clone ID:
ccsbBroadEn_07487
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC043345.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516492
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGGCTAAGGACCAGAAGAAACACAGAAAGAAAAACAGTGGACCCAAAGCTGCAAAG
AAAAAGAAGCGGCTTCTGCAGGATCTCCAGCTAGGAGACGAAGAAGATGCCCGGAAGAGA
AATCCCAAAGCTTTTGCAGTTCAGTCTGCTGTGCGGATGGCTCGATCCTTTCACAGGACT
CAGGATTTGAAGACAAAAAAGCATCATATTCCAGTGGTTGATCGAACTCCACTAGAGCCC
CCACCAATAGTGGTAGTGGTGATGGGACCTCCAAAAGTTGGAAAGAGCACTTTGATACAA
TGCCTCATTCGGAACTTTACCCGGCAGAAGTTGACCGAGATCAGAGGCCCTGTGACGATT
GTGTCAGGTAAAAAGCGCAGACTCACCATTATTGAATGTGGGTGTGACATTAACATGATG
ATTGATCTGGCTAAAGTAGCAGATCTGGTACTGATGCTTATAGATGCCAGCTTTGGGTTT
GAAATGGAAACGTTTGAGTTTCTAAACATCTGTCAAGTACATGGCTTTCCTAAAATTATG
GGAGTTCTCACCCACCTCGACTCCTTCAAGCATAATAAGCAACTGAAGAAGACAAAGAAG
CGATTAAAACACAGGTTCTGGACGGAAGTTTACCCGGGTGCCAAGCTGTTCTACCTTTCT
GGAATGGTGCATGGAGAATATCAAAACCAAGAAATCCACAATCTGGGCCGTTTTATTACA
GTTATGAAGTTTAGGCCTCTCACATGGCAAACTTCTCACCCTTATATCCTGGCAGACAGG
ATGGAAGATTTGACAAACCCAGAGGATATCCGAACAAACATCAAATGTGACCGGAAGGTG
TCACTTTATGGTTATTTAAGAGGAGCACACTTGAAAAATAAAAGCCAAATTCACATGCCA
GGGGTAGGAGATTTTGCCGTGAGTGACATCAGTTTCCTCCCAGACCCTTGCGCTCTTCCT
GAACAACAAAAGAAGCGCTGTTTAAATGAGAAGGAGAAGCTGGTTTATGCGCCTCTTTCT
GGAGTTGGGGGTGTGCTGTATGACAAAGACGCTGTCTATGTTGACCTTGGTGGCAGCCAC
GTTTTTCAGGATGAAGTGGGGCCCACCCATGAGCTGGTCCAGAGTCTCATCTCTACCCAC
TCCACCATTGATGCCAAGATGGCTTCAAGTCGAGTGACGCTGTTTTCTGATTCCAAGCCA
CTTGGGTCAGAGGATATAGATAATCAAGGGCTAATGATGCCAAAGGAGGAAAAACAAATG
GACTTGAACACTGGTCGAATGCGTCGGAAAGCCATTTTCGGAGATGAAGATGAATCTGGA
GATAGTGATGATGAAGAAGATGATGAAATGTCTGAAGATGACGGGTTGGAAAACGGCTCT
AGTGATGAGGAAGCAGAAGAGGAGGAAAATGCTGAGATGACTGATCAGTATATGGCTGTT
AAGGGCATCAAACGACGGAAACTTGAGTTGGAAGAAGACAGTGAAATGGATTTGCCAGCA
TTTGCTGACAGTGACGATGACCTTGAGAGGAGCTCAGCGGAAGAAGGGGAAGCGGAGGAA
GCTGATGAAAGCAGTGAAGAAGAGGACTGCACTGCAGGAGAGAAGGGCATTTCAGGATCA
AAGGCTGCTGGAGAAGGTAGTAAAGCAGGGCTGTCACCAGCTAATTGCCAGAGTGACCGT
GTGAATCTGGAGAAGTCTTTGCTGATGAAGAAAGCAGCTCTCCCCACTTTCGATTCTGGG
CATTGCACAGCTGAAGAGGTGTTTGCATCTGAAGATGAATCTGAAGAAAGCTCCTCACTC
AGTGCAGAGGAAGAAGACTCAGAAAATGAAGAGGCTATTAGAAAAAAGCTTTCAAAGCCT
TCTCAAGTGAGCAGTGGTCAGAAACTGGGGCCACAGAACTTCATTGATGAGACCAGTGAT
ATAGAAAATTTACTCAAAGAGGAAGAAGATTACAAGGAAGAAAATAATGATTCCAAAGAA
ACGTCAGGTGCCCTCAAGTGGAAGGAAGACCTTTCCAGAAAGGCAGCTGAGGCCTTTCTG
AGGCAGCAGCAAGCAGCTCCAAACCTCCGAAAGCTTATTTATGGGACAGTGACAGAAGAT
AATGAAGAAGAAGATGATGATACTCTAGAAGAGCTTGGAGGGTTGTTTCGTGTCAACCAG
CCTGACAGAGAGTGTAAGCACAAGGCTGACTCTTTGGACTGCTCCAGATTTCTTGTGGAG
GCCCCCCATGACTGGGATTTAGAGGAGGTTATGAACAGTATCAGAGATTGCTTCGTGACT
GGAAAGTGGGAAGATGATAAAGATGCAGCCAAGGTCTTAGCAGAAGATGAGGAGCTCTAC
GGTGACTTTGAAGACTTGGAAACAGGGGACGTGCACAAGGGAAAATCAGGCCCCAATACT
CAGAATGAAGATATAGAGAAAGAAGTTAAGGAAGAAATTGACCCTGACGAAGAAGAAAGT
GCCAAGAAAAAGCATTTGGATAAGAAGAGAAAATTGAAGGAGATGTTTGATGCAGAATAT
GATGAAGGAGAAAGCACATATTTTGATGATCTTAAAGGAGAAATGCAGAAACAAGCACAG
CTGAATCGCGCAGAATTTGAAGATCAAGATGATGAAGCCAGAGTTCAGTATGAGGGTTTT
CGACCTGGGATGTACGTCCGCATTGAGATTGAAAATGTTCCCTGTGAATTTGTGCAGAAC
TTTGACCCCCATTACCCCATTATCCTGGGTGGCTTGGGCAACAGTGAGGGAAATGTTGGC
TACGTGCAGATGCGTCTGAAGAAACATCGCTGGTATAAGAAAATCCTCAAGTCCCGAGAT
CCAATCATATTTTCTGTAGGGTGGAGGAGGTTTCAGACCATCCCACTGTATTATATCGAA
GACCACAATGGAAGACAAAGGCTTCTAAAGTATACCCCACAGCACATGCATTGCGGAGCA
GCCTTTTGGGGCCCTATCACTCCACAGGGAACTGGTTTCTTGGCAATACAGTCTGTCAGT
GGCATAATGCCTGATTTTCGGATAGCTGCTACAGGAGTTGTCCTTGATCTGGATAAATCC
ATAAAAATTGTGAAGAAATTAAAGCTAACTGGTTTTCCATATAAAATTTTCAAGAACACT
TCATTTATTAAGGGAATGTTTAATTCTGCCTTGGAAGTGGCCAAATTTGAAGGTGCTGTG
ATTCGAACAGTCAGTGGGATAAGGGGGCAGATCAAGAAAGCACTCCGAGCTCCAGAAGGA
GCTTTCAGGGCCAGCTTTGAGGATAAGCTGCTGATGAGCGATATTGTCTTCATGCGAACT
TGGTATCCTGTTTCCATCCCAGCGTTCTATAACCCAGTAACATCTTTGTTGAAACCAGTG
GGTGAGAAAGACACCTGGTCAGGAATGCGGACCACGGGCCAACTCAGGCTCGCCCATGGC
ATCAGACTAAAGGCGAACAAGGACTCTCTGTATAAGCCAATCCTGAGGCAAAAGAAACAT
TTTAATTCACTGCACATTCCAAAAGCCTTGCAGAAGGCCCTGCCATTTAAGAACAAGCCC
AAGACCCAAGCAAAGGCAGGCAAGGTGCCAAAGGACAGGCGGAGACCGGCCGTCATACGC
GAGCCTCATGAAAGAAAGATCCTTGCACTGCTGGATGCTCTGAGTACGGTGCATAGTCAG
AAGATGAAGAAGGCCAAGGAGCAGCGGCACCTGCACAATAAAGAGCACTTCAGAGCCAAG
CAGAAGGAGGAGGAGGAGAAGCTGAAGCGGCAGAAGGACCTCAGGAAGAAGCTCTTCAGA
ATTCAGGGGCAGAAGGAAAGAAGAAACCAGAAGTCCAGTTTGAAGGGGGCTGAGGGCCAA
TTGCAG
Protein sequence predicted based on nucleotide sequence
MEAKDQKKHRKKNSGPKAAKKKKRLLQDLQLGDEEDARKRNPKAFAVQSAVRMARSFHRT QDLKTKKHHIPVVDRTPLEPPPIVVVVMGPPKVGKSTLIQCLIRNFTRQKLTEIRGPVTI VSGKKRRLTIIECGCDINMMIDLAKVADLVLMLIDASFGFEMETFEFLNICQVHGFPKIM GVLTHLDSFKHNKQLKKTKKRLKHRFWTEVYPGAKLFYLSGMVHGEYQNQEIHNLGRFIT VMKFRPLTWQTSHPYILADRMEDLTNPEDIRTNIKCDRKVSLYGYLRGAHLKNKSQIHMP GVGDFAVSDISFLPDPCALPEQQKKRCLNEKEKLVYAPLSGVGGVLYDKDAVYVDLGGSH VFQDEVGPTHELVQSLISTHSTIDAKMASSRVTLFSDSKPLGSEDIDNQGLMMPKEEKQM DLNTGRMRRKAIFGDEDESGDSDDEEDDEMSEDDGLENGSSDEEAEEEENAEMTDQYMAV KGIKRRKLELEEDSEMDLPAFADSDDDLERSSAEEGEAEEADESSEEEDCTAGEKGISGS KAAGEGSKAGLSPANCQSDRVNLEKSLLMKKAALPTFDSGHCTAEEVFASEDESEESSSL SAEEEDSENEEAIRKKLSKPSQVSSGQKLGPQNFIDETSDIENLLKEEEDYKEENNDSKE TSGALKWKEDLSRKAAEAFLRQQQAAPNLRKLIYGTVTEDNEEEDDDTLEELGGLFRVNQ PDRECKHKADSLDCSRFLVEAPHDWDLEEVMNSIRDCFVTGKWEDDKDAAKVLAEDEELY GDFEDLETGDVHKGKSGPNTQNEDIEKEVKEEIDPDEEESAKKKHLDKKRKLKEMFDAEY DEGESTYFDDLKGEMQKQAQLNRAEFEDQDDEARVQYEGFRPGMYVRIEIENVPCEFVQN FDPHYPIILGGLGNSEGNVGYVQMRLKKHRWYKKILKSRDPIIFSVGWRRFQTIPLYYIE DHNGRQRLLKYTPQHMHCGAAFWGPITPQGTGFLAIQSVSGIMPDFRIAATGVVLDLDKS IKIVKKLKLTGFPYKIFKNTSFIKGMFNSALEVAKFEGAVIRTVSGIRGQIKKALRAPEG AFRASFEDKLLMSDIVFMRTWYPVSIPAFYNPVTSLLKPVGEKDTWSGMRTTGQLRLAHG IRLKANKDSLYKPILRQKKHFNSLHIPKALQKALPFKNKPKTQAKAGKVPKDRRRPAVIR EPHERKILALLDALSTVHSQKMKKAKEQRHLHNKEHFRAKQKEEEEKLKRQKDLRKKLFR IQGQKERRNQKSSLKGAEGQLQ
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
