Gene:
Gene Symbol:
CDHR2
Gene Name:
cadherin-related family member 2
Original Clone ID:
ccsbBroadEn_08415
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC130282.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516493
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCCAGCTATGGCTGTCCTGCTTCCTCCTTCCTGCCCTCGTGGTGTCTGTGGCAGCC
AACGTGGCCCCGAAGTTCCTAGCCAACATGACGTCAGTGATCCTGCCTGAGGACCTGCCT
GTGGGTGCCCAGGCCTTCTGGTTGGTAGCGGAAGACCAGGACAATGACCCTCTGACCTAT
GGGATGAGCGGCCCCAATGCCTACTTCTTCGCTGTCACTCCGAAAACTGGGGAAGTGAAG
CTGGCCAGCGCTCTGGACTACGAGACACTCTACACATTCAAAGTCACCATCTCCGTGAGC
GACCCCTACATCCAGGTGCAGAGGGAGATGCTGGTGATTGTGGAAGATAGAAACGACAAC
GCACCCGTTTTCCAGAACACCGCTTTCTCCACCAGCATCAACGAGACCCTGCCCGTGGGC
AGTGTGGTGTTCTCCGTGCTGGCCGTGGATAAAGACATGGGGTCTGCAGGCATGGTCGTG
TACTCCATAGAGAAGGTCATCCCTAGCACTGGGGACAGCGAGCATCTCTTCCGGATCCTG
GCCAATGGCTCCATCGTCCTCAATGGCAGCCTCAGCTACAACAACAAGAGCGCTTTCTAC
CAGCTGGAGCTGAAGGCCTGTGACTTGGGCGGCATGTACCACAACACCTTCACCATCCAG
TGCTCCCTGCCTGTCTTCCTGTCCATCTCCGTGGTGGACCAGCCTGACCTTGACCCCCAG
TTTGTCAGGGAGTTTTACTCGGCCTCTGTGGCTGAGGATGCAGCCAAGGGAACCTCGGTG
CTGACGGTGGAGGCTGTGGATGGCGACAAAGGCATCAATGACCCTGTGATCTACAGCATC
TCCTACTCCACGCGGCCCGGCTGGTTTGACATCGGGGCAGATGGGGTGATCAGGGTCAAC
GGCTCCCTGGACCGTGAGCAGCTGCTGGAGGCGGATGAGGAGGTGCAGCTGCAGGTCACG
GCCACCGAGACACACCTCAACATCTACGGGCAGGAGGCCAAGGTGAGCATCTGGGTGACA
GTGAGAGTGATGGACGTCAATGACCACAAACCTGAGTTTTACAACTGCAGCCTCCCAGCC
TGCACCTTCACCCCCGAAGAGGCCCAAGTGAACTTCACTGGCTACGTGGACGAGCATGCC
TCCCCCCGCATCCCCATCGATGACCTCACCATGGTGGTCTACGACCCGGACAAGGGCAGC
AATGGCACCTTCCTGTTGTCGCTGGGGGGCCCCGATGCAGAAGCCTTCAGCGTCTCCCCG
GAGCGGGCAGCGGGCTCAGCCTCCGTTCAGGTGCTGGTGAGAGTATCCGCGCTGGTGGAC
TACGAGAGGCAGACGGCGATGGCGGTGCAGGTTGTGGCCACAGACTCCGTCAGCCAGAAC
TTCTCCGTCGCCATGGTGACCATCCACCTTAGAGACATTAATGACCACAGGCCCACGTTT
CCCCAGAGCTTGTACGTCCTCACGGTGCCAGAGCACAGCGCCACCGGCTCTGTGGTCACC
GACAGCATCCACGCCACGGACCCAGACACGGGCGCGTGGGGCCAAATTACCTACAGCCTG
CTCCCAGGAAATGGGGCAGACCTCTTCCAAGTGGATCCCGTCTCAGGGACGGTGACGGTG
AGGAACGGTGAGCTGCTGGACCGGGAGAGCCAGGCCGTGTACTACCTGACGCTGCAGGCC
ACAGATGGCGGGAACCTGTCCTCCTCCACCACACTGCAGATCCACCTGCTGGACATCAAC
GACAATGCACCCGTGGTTAGCGGCTCCTACAACATCTTCGTCCAGGAGGAGGAGGGCAAT
GTCTCCGTGACCATCCAGGCCCACGACAATGATGAGCCGGGCACCAACAACAGCCGTCTG
CTCTTCAACCTGCTGCCTGGCCCCTACAGCCACAACTTCTCCTTGGACCCTGACACAGGG
CTCCTCAGAAACCTGGGGCCCCTGGACAGAGAGGCCATCGACCCCGCCCTGGAGGGCCGC
ATTGTGCTGACAGTGCTTGTGTCTGACTGCGGCGAGCCTGTCCTCGGCACCAAAGTCAAT
GTCACCATCACTGTGGAGGACATCAATGATAACCTGCCCATCTTCAATCAGTCCAGCTAC
AACTTTACGGTGAAGGAGGAGGATCCAGGAGTGCTAGTGGGCGTGGTGAAGGCCTGGGAC
GCGGACCAGACGGAAGCCAACAACCGCATCAGCTTCAGCCTGTCGGGGAGTGGTGCCAAC
TACTTCATGATCCGAGGCTTGGTGCTGGGGGCTGGGTGGGCTGAGGGCTACCTCCGGCTG
CCCCCGGACGTGAGCCTGGATTACGAGACACAGCCCGTCTTCAACTTGACAGTGAGTGCT
GAGAACCCAGACCCCCAGGGGGGTGAGACCATAGTAGACGTCTGCGTGAATGTGAAAGAC
GTGAACGACAATCCCCCCACCCTGGATGTAGCCTCACTCCGGGGCATCCGTGTGGCTGAG
AATGGCTCACAGCACGGCCAGGTGGCTGTGGTGGTTGCCTCGGATGTGGACACCAGTGCC
CAGCTGGAGATACAGCTTGTGAACATTCTCTGCACCAAGGCCGGGGTCGATGTGGGCAGC
CTATGCTGGGGCTGGTTCTCAGTGGCGGCCAACGGCTCTGTGTACATCAACCAGAGCAAA
GCCATCGACTACGAGGCCTGTGACCTGGTCACGCTGGTTGTGCGGGCCTGTGACCTAGCC
ACGGACCCCGGCTTCCAGGCCTACAGCAACAATGGAAGCCTCCTCATTACCATTGAGGAC
GTGAATGACAATGCACCCTATTTTCTGCCTGAGAATAAGACTTTTGTGATCATCCCTGAA
CTCGTGCTGCCCAACCGGGAGGTGGCTTCTGTCCGGGCCAGAGACGATGATTCAGGGAAC
AATGGCGTCATCCTGTTCTCCATCCTCCGAGTAGACTTCATCTCTAAGGACGGGGCCACC
ATCCCTTTCCAGGGTGTCTTCTCGATCTTCACCTCCTCCGAGGCCGACGTGTTCGCTGGG
AGCATTCAGCCGGTGACCAGCCTCGACTCCACTCTCCAAGGCACCTACCAAGTGACAGTC
CAGGCCAGGGACAGACCTTCCTTGGGTCCTTTCCTGGAAGCCACCACCACCCTGAATCTC
TTCACCGTGGACCAGAGTTACCGCTCGCGGCTGCAGTTCTCCACACCGAAGGAGGAGGTG
GGCGCCAACAGACAGGCGATTAATGCGGCTCTTACCCAGGCAACCAGGACTACAGTATAC
ATTGTGGACATTCAGGACATAGATTCTGCAGCTCGGGCCCGACCTCACTCCTACCTCGAT
GCCTACTTTGTCTTCCCCAATGGGTCAGCCCTGACCCTTGATGAGCTGAGTGTGATGATC
CGGAATGATCAGGACTCGCTGATGCAGCTGCTGCAGCTGGGGCTGGTGGTGCTGGGCTCC
CAGGAGAGCCAGGAGTCAGACCTGTCGAAACAGCTCATCAGTGTCATCATAGGATTGGGA
GTGGCTTTGCTGCTGGTCCTTGTGATCATGACCATGGCCTTCGTGTGTGTGCGGAAGAGC
TACAACCGGAAGCTTCAAGCTATGAAGGCTGCCAAGGAGGCCAGGAAGACAGCAGCAGGG
GTGATGCCCTCAGCCCCTGCCATCCCAGGGACTAACATGTACAACACTGAGCGAGCCAAC
CCCATGCTGAACCTCCCCAACAAAGACCTGGGCTTGGAGTACCTCTCTCCCTCCAATGAC
CTGGACTCTGTCAGCGTCAACTCCCTGGACGACAACTCTGTGGATGTGGACAAGAACAGT
CAGGAAATCAAGGAGCACAGGCCACCACACACACCACCAGAGCCAGATCCAGAGCCCCTG
AGCGTGGTCCTGTTAGGACGGCAGGCAGGCGCAAGTGGACAGCTGGAGGGGCCATCCTAC
ACCAACGCTGGCCTGGACACCACGGACCTG
Protein sequence predicted based on nucleotide sequence
MAQLWLSCFLLPALVVSVAANVAPKFLANMTSVILPEDLPVGAQAFWLVAEDQDNDPLTY GMSGPNAYFFAVTPKTGEVKLASALDYETLYTFKVTISVSDPYIQVQREMLVIVEDRNDN APVFQNTAFSTSINETLPVGSVVFSVLAVDKDMGSAGMVVYSIEKVIPSTGDSEHLFRIL ANGSIVLNGSLSYNNKSAFYQLELKACDLGGMYHNTFTIQCSLPVFLSISVVDQPDLDPQ FVREFYSASVAEDAAKGTSVLTVEAVDGDKGINDPVIYSISYSTRPGWFDIGADGVIRVN GSLDREQLLEADEEVQLQVTATETHLNIYGQEAKVSIWVTVRVMDVNDHKPEFYNCSLPA CTFTPEEAQVNFTGYVDEHASPRIPIDDLTMVVYDPDKGSNGTFLLSLGGPDAEAFSVSP ERAAGSASVQVLVRVSALVDYERQTAMAVQVVATDSVSQNFSVAMVTIHLRDINDHRPTF PQSLYVLTVPEHSATGSVVTDSIHATDPDTGAWGQITYSLLPGNGADLFQVDPVSGTVTV RNGELLDRESQAVYYLTLQATDGGNLSSSTTLQIHLLDINDNAPVVSGSYNIFVQEEEGN VSVTIQAHDNDEPGTNNSRLLFNLLPGPYSHNFSLDPDTGLLRNLGPLDREAIDPALEGR IVLTVLVSDCGEPVLGTKVNVTITVEDINDNLPIFNQSSYNFTVKEEDPGVLVGVVKAWD ADQTEANNRISFSLSGSGANYFMIRGLVLGAGWAEGYLRLPPDVSLDYETQPVFNLTVSA ENPDPQGGETIVDVCVNVKDVNDNPPTLDVASLRGIRVAENGSQHGQVAVVVASDVDTSA QLEIQLVNILCTKAGVDVGSLCWGWFSVAANGSVYINQSKAIDYEACDLVTLVVRACDLA TDPGFQAYSNNGSLLITIEDVNDNAPYFLPENKTFVIIPELVLPNREVASVRARDDDSGN NGVILFSILRVDFISKDGATIPFQGVFSIFTSSEADVFAGSIQPVTSLDSTLQGTYQVTV QARDRPSLGPFLEATTTLNLFTVDQSYRSRLQFSTPKEEVGANRQAINAALTQATRTTVY IVDIQDIDSAARARPHSYLDAYFVFPNGSALTLDELSVMIRNDQDSLMQLLQLGLVVLGS QESQESDLSKQLISVIIGLGVALLLVLVIMTMAFVCVRKSYNRKLQAMKAAKEARKTAAG VMPSAPAIPGTNMYNTERANPMLNLPNKDLGLEYLSPSNDLDSVSVNSLDDNSVDVDKNS QEIKEHRPPHTPPEPDPEPLSVVLLGRQAGASGQLEGPSYTNAGLDTTDL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
