Gene:
Gene Symbol:
KIF21B
Gene Name:
kinesin family member 21B
Original Clone ID:
ccsbBroadEn_11664
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC137281.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516503
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCGGCCAGGGGGACTGCTGCGTCAAGGTGGCCGTCAGGATCCGGCCCCAGCTGTCG
AAGGAGAAGATTGAGGGCTGTCACATCTGTACCTCTGTTACCCCGGGAGAGCCCCAGGTC
CTGCTGGGGAAGGACAAGGCCTTCACCTATGACTTTGTCTTCGACCTGGACACCTGGCAA
GAACAGATCTATTCCACCTGTGTGAGCAAGCTCATCGAGGGCTGCTTCGAGGGCTATAAT
GCCACGGTGCTGGCCTATGGGCAGACGGGGGCCGGGAAGACGTACACCATGGGCACTGGC
TTTGACATGGCAACGTCGGAGGAGGAGCAGGGCATCATCCCGAGGGCCATCGCACACCTC
TTTGGGGGCATTGCCGAGCGCAAGCGCCGGGCACAGGAGCAGGGCGTGGCTGGACCTGAG
TTCAAAGTCAGCGCCCAGTTTCTGGAGCTCTACAACGAGGAGATCCTTGACCTGTTTGAC
AGCACCCGTGACCCTGACACCCGCCACCGCAGGTCCAACATCAAGATCCACGAGGACGCA
AACGGTGGCATCTACACCACTGGCGTCACTTCTCGCCTCATCCACTCCCAGGAGGAGCTG
ATCCAGTGCCTGAAGCAGGGGGCCCTGTCCCGCACCACAGCCAGCACCCAGATGAACGTG
CAGAGCTCACGCTCCCACGCCATCTTCACCATCCACCTGTGCCAGATGCGCATGTGCACC
CAGCCCGACCTGGTGAATGAGGCGGTGACTGGGCTTCCTGATGGTACACCTCCCTCGAGT
GAGTATGAGACACTCACTGCTAAGTTTCACTTTGTGGACCTGGCCGGCTCAGAGCGGCTG
AAGCGGACAGGGGCTACTGGCGAGCGGGCCAAGGAGGGCATCTCCATCAACTGTGGCCTG
CTGGCCTTGGGCAATGTGATCAGCGCCTTAGGGGACCAGAGCAAGAAGGTGGTGCACGTT
CCCTACAGGGACTCCAAGCTCACTCGGCTCCTCCAGGATTCGCTGGGGGGCAACAGCCAG
ACCATCATGATCGCCTGTGTGAGCCCCTCAGACCGAGATTTCATGGAGACCCTCAACACA
CTCAAATATGCCAATCGGGCCCGCAACATCAAGAACAAGGTGGTAGTGAACCAGGACAAG
ACCAGCCAGCAAATCAGTGCACTGCGGGCTGAGATTGCTCGGCTGCAGATGGAGCTGATG
GAGTATAAGGCGGGCAAGCGAGTGATAGGAGAGGATGGCGCTGAGGGCTATAGTGATCTG
TTCCGAGAGAATGCCATGCTACAGAAGGAGAATGGGGCCCTGCGGCTGCGGGTGAAAGCC
ATGCAGGAGGCCATCGATGCCATCAACAACCGCGTCACCCAGCTCATGAGCCAGGAGGCC
AACCTGCTGCTAGCCAAGGCCGGCGATGGCAATGAGGCCATTGGTGCGCTGATCCAGAAC
TACATCCGGGAGATCGAGGAGCTACGGACTAAGCTTCTAGAGAGTGAAGCCATGAACGAG
TCCCTGCGCCGCAGCCTCTCACGGGCCTCGGCTAGGAGCCCCTACTCCCTGGGTGCTTCT
CCAGCCGCCCCGGCCTTCGGGGGCAGCCCTGCCAGCTCCATGGAGGATGCCTCGGAGGTG
ATCCGCAGGGCCAAGCAGGACCTGGAGCGGCTAAAGAAGAAGGAGGTCAGGCAGCGGAGG
AAGAGCCCCGAGAAGGAAGCCTTCAAAAAGAGGGCAAAACTCCAACAGGAGAACAGCGAG
GAGACGGATGAGAACGAGGCGGAGGAGGAGGAGGAAGAGCGAGACGAGAGTGGCTGTGAG
GAGGAGGAAGGGCGCGAGGATGAAGATGAGGACTCGGGCAGTGAAGAGAGCCTGGTGGAC
TCAGACTCAGACCCCGAGGAGAAGGAGGTGAACTTCCAGGCGGACCTGGCCGACCTGACT
TGTGAGATCGAAATCAAGCAGAAGCTGATCGACGAGCTGGAGAACAGCCAGCGGCGGTTG
CAGACGCTCAAGCACCAGTATGAGGAAAAGCTGATTCTGCTGCAGAACAAGATCCGAGAC
ACACAGCTGGAGCGCGACCGTGTGCTGCAGAACCTCAGCACCATGGAGTGCTATACTGAG
GAGAAGGCCAACAAGATCAAGGCAGACTATGAGAAGAGGCTGCGGGAGATGAACCGGGAC
CTGCAGAAGCTGCAGGCCGCCCAGAAAGAGCACGCCCGGCTGCTTAAGAACCAGTCGCGC
TACGAGAGGGAGCTGAAGAAGCTACAGGCCGAGGTGGCTGAGATGAAGAAGGCCAAGGTG
GCCCTGATGAAGCAGATGCGTGAGGAGCAACAGCGGCGGCGGCTAGTGGAGACCAAGAGG
AACCGGGAGATCGCACAGCTCAAGAAGGAGCAGCGGCGACAGGAGTTTCAGATCCGAGCT
CTGGAGTCCCAGAAGCGGCAGCAGGAGATGGTCCTGAGGAGGAAGACCCAGGAGGTTTCT
GCACTGAGGCGCCTGGCCAAGCCCATGTCTGAGCGGGTGGCAGGGCGTGCAGGACTAAAG
CCACCCATGCTGGACTCTGGGGCTGAGGTGTCGGCCAGCACTACCTCATCTGAGGCTGAA
TCAGGGGCCCGCTCTGTCTCCAGCATCGTGCGCCAGTGGAACCGCAAAATCAACCACTTC
TTGGGGGACCATCCTGCGCCCACTGTCAATGGCACCCGTCCTGCCCGAAAGAAGTTCCAG
AAGAAGGGGGCCAGCCAGAGCTTCAGTAAGGCGGCAAGGCTCAAGTGGCAGTCCCTGGAG
CGACGGATCATTGACATCGTCATGCAGAGAATGACCATTGTCAACCTGGAGGCTGACATG
GAGCGGCTCATCAAGAAAAGGGAGGAGCTGTTCCTCCTGCAGGAGGCACTGCGGAGGAAG
CGGGAGCGGCTGCAGGCTGAGAGCCCCGAGGAAGAGAAGGGGCTGCAGGAGCTGGCTGAG
GAGATCGAGGTGCTGGCAGCCAACATTGACTACATCAATGACGGCATCACCGACTGCCAG
GCCACCATCGTGCAGCTGGAGGAGACCAAGGAGGAGCTGGACTCCACAGACACATCCGTG
GTCATCAGCTCCTGCTCCCTGGCTGAAGCCCGCCTCCTGCTAGACAACTTCCTCAAGGCA
TCCATTGACAAGGGGCTGCAAGTGGCACAAAAGGAAGCCCAGATCCGGCTGTTGGAGGGC
CGACTGAGGCAGACGGATATGGCAGGCTCCTCCCAGAACCATCTGCTCCTGGACGCCCTG
CGTGAGAAGGCTGAAGCTCACCCCGAGCTGCAGGCCCTCATCTACAATGTGCAGCAGGAG
AATGGCTACGCCAGCACAGATGAGGAGATCTCAGAGTTCTCTGAGGGCAGCTTCTCCCAG
TCATTCACCATGAAAGGCTCCACCAGCCATGACGATTTCAAGTTCAAGAGCGAGCCCAAA
CTGTCTGCCCAAATGAAAGCTGTGTCGGCTGAGTGCCTGGGCCCCCCACTGGATATCTCC
ACCAAGAACATCACCAAGTCCCTGGCCTCCCTCGTTGAGATCAAAGAGGACGGAGTGGGC
TTCTCTGTCCGAGACCCCTATTACCGGGACAGGGTCTCGCGCACCGTCAGTCTGCCTACC
CGGGGCAGCACTTTCCCTAGGCAATCTCGAGCCACAGAGACGTCCCCGCTGACGAGAAGG
AAGTCCTACGACCGAGGGCAGCCCATTAGGTCCACAGATGTGGGATTCACACCCCCATCA
TCCCCTCCCACTCGGCCCCGCAATGACCGCAATGTCTTCTCTCGTCTCACCAGTAATCAG
AGCCAGGGGTCAGCGCTGGACAAGTCTGATGACAGCGACTCCTCTTTGTCGGAGGTCCTG
AGGGGCATCATCTCCCCGGTTGGAGGAGCCAAGGGTGCACGGACGGCCCCACTGCAGTGT
GTCTCCATGGCCGAAGGCCACACCAAGCCCATCCTCTGCCTGGATGCCACAGATGAGTTG
CTATTCACAGGGTCCAAAGACCGAAGCTGCAAGATGTGGAACTTGGTTACGGGACAGGAG
ATCGCAGCTCTAAAGGGCCACCCCAACAACGTGGTCTCCATCAAGTACTGCAGCCACTCG
GGGCTTGTGTTCTCCGTGTCCACCTCCTACATCAAGGTGTGGGACATCCGGGACTCAGCC
AAGTGCATTCGGACTCTCACGTCCTCGGGCCAGGTGATCTCAGGGGATGCCTGTGCCGCC
ACATCCACCCGTGCCATCACCAGTGCTCAGGGCGAGCATCAGATCAACCAGATCGCCCTC
AGCCCTTCGGGCACCATGCTGTACGCCGCCTCGGGCAATGCCGTCCGCATCTGGGAGCTT
AGCAGGTTCCAGCCTGTCGGCAAGCTGACTGGCCACATCGGCCCTGTGATGTGCCTGACG
GTCACCCAGACGGCCAGCCAGCATGACCTCGTGGTGACTGGCTCCAAGGACCACTACGTT
AAGATGTTCGAGCTGGGCGAGTGTGTGACGGGCACCATCGGCCCCACTCACAACTTCGAG
CCCCCGCACTACGATGGCATCGAGTGTCTCGCCATCCAGGGAGACATCCTGTTCAGTGGC
TCCCGAGATAACGGCATCAAGAAGTGGGACCTAGACCAGCAGGAGCTCATCCAGCAAATC
CCCAATGCGCACAAGGACTGGGTGTGCGCCCTGGCCTTCATCCCGGGCCGCCCCATGCTG
CTCAGCGCCTGCCGTGCGGGTGTCATCAAGGTCTGGAACGTGGACAACTTCACACCCATC
GGTGAGATCAAGGGCCACGACAGTCCCATCAATGCCATCTGCACCAATGCCAAGCATATC
TTCACAGCCTCCAGTGACCTGACGGTGAAGTTCTGGAGTGTCCGGCGGTTACCCCACAGC
GGCCCACCC
Protein sequence predicted based on nucleotide sequence
MAGQGDCCVKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLLGKDKAFTYDFVFDLDTWQ EQIYSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDMATSEEEQGIIPRAIAHL FGGIAERKRRAQEQGVAGPEFKVSAQFLELYNEEILDLFDSTRDPDTRHRRSNIKIHEDA NGGIYTTGVTSRLIHSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLCQMRMCT QPDLVNEAVTGLPDGTPPSSEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGL LALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNT LKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDL FRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAIGALIQN YIREIEELRTKLLESEAMNESLRRSLSRASARSPYSLGASPAAPAFGGSPASSMEDASEV IRRAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCE EEEGREDEDEDSGSEESLVDSDSDPEEKEVNFQADLADLTCEIEIKQKLIDELENSQRRL QTLKHQYEEKLILLQNKIRDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRD LQKLQAAQKEHARLLKNQSRYERELKKLQAEVAEMKKAKVALMKQMREEQQRRRLVETKR NREIAQLKKEQRRQEFQIRALESQKRQQEMVLRRKTQEVSALRRLAKPMSERVAGRAGLK PPMLDSGAEVSASTTSSEAESGARSVSSIVRQWNRKINHFLGDHPAPTVNGTRPARKKFQ KKGASQSFSKAARLKWQSLERRIIDIVMQRMTIVNLEADMERLIKKREELFLLQEALRRK RERLQAESPEEEKGLQELAEEIEVLAANIDYINDGITDCQATIVQLEETKEELDSTDTSV VISSCSLAEARLLLDNFLKASIDKGLQVAQKEAQIRLLEGRLRQTDMAGSSQNHLLLDAL REKAEAHPELQALIYNVQQENGYASTDEEISEFSEGSFSQSFTMKGSTSHDDFKFKSEPK LSAQMKAVSAECLGPPLDISTKNITKSLASLVEIKEDGVGFSVRDPYYRDRVSRTVSLPT RGSTFPRQSRATETSPLTRRKSYDRGQPIRSTDVGFTPPSSPPTRPRNDRNVFSRLTSNQ SQGSALDKSDDSDSSLSEVLRGIISPVGGAKGARTAPLQCVSMAEGHTKPILCLDATDEL LFTGSKDRSCKMWNLVTGQEIAALKGHPNNVVSIKYCSHSGLVFSVSTSYIKVWDIRDSA KCIRTLTSSGQVISGDACAATSTRAITSAQGEHQINQIALSPSGTMLYAASGNAVRIWEL SRFQPVGKLTGHIGPVMCLTVTQTASQHDLVVTGSKDHYVKMFELGECVTGTIGPTHNFE PPHYDGIECLAIQGDILFSGSRDNGIKKWDLDQQELIQQIPNAHKDWVCALAFIPGRPML LSACRAGVIKVWNVDNFTPIGEIKGHDSPINAICTNAKHIFTASSDLTVKFWSVRRLPHS GPP
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
